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O65361

- P5CS_MESCR

UniProt

O65361 - P5CS_MESCR

Protein

Delta-1-pyrroline-5-carboxylate synthase

Gene

P5CS

Organism
Mesembryanthemum crystallinum (Common ice plant) (Cryophytum crystallinum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.

    Catalytic activityi

    ATP + L-glutamate = ADP + L-glutamate 5-phosphate.
    L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.

    Enzyme regulationi

    Feedback regulated by proline.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei57 – 571SubstrateBy similarity
    Binding sitei154 – 1541SubstrateBy similarity
    Binding sitei173 – 1731Substrate; via amide nitrogenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi193 – 1942ATPBy similarity
    Nucleotide bindingi233 – 2397ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate 5-kinase activity Source: UniProtKB-EC
    3. glutamate-5-semialdehyde dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. L-proline biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Oxidoreductase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Proline biosynthesis

    Keywords - Ligandi

    ATP-binding, NADP, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00098; UER00359.
    UPA00098; UER00360.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-1-pyrroline-5-carboxylate synthase
    Short name:
    P5CS
    Including the following 2 domains:
    Glutamate 5-kinase (EC:2.7.2.11)
    Short name:
    GK
    Alternative name(s):
    Gamma-glutamyl kinase
    Gamma-glutamyl phosphate reductase (EC:1.2.1.41)
    Short name:
    GPR
    Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
    Gene namesi
    Name:P5CS
    OrganismiMesembryanthemum crystallinum (Common ice plant) (Cryophytum crystallinum)
    Taxonomic identifieri3544 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAizoaceaeMesembryanthemum

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 719719Delta-1-pyrroline-5-carboxylate synthasePRO_0000109776Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed at high levels in leaves and is inducible in roots subjected to salt stress.

    Structurei

    3D structure databases

    ProteinModelPortaliO65361.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 293293Glutamate 5-kinaseAdd
    BLAST
    Regioni294 – 719426Gamma-glutamyl phosphate reductaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the glutamate 5-kinase family.Curated
    In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.Curated

    Family and domain databases

    Gene3Di3.40.1160.10. 1 hit.
    3.40.309.10. 1 hit.
    3.40.605.10. 2 hits.
    HAMAPiMF_00412. ProA.
    MF_00456. ProB.
    InterProiIPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR001048. Asp/Glu/Uridylate_kinase.
    IPR000965. G-glutamylP_reductase.
    IPR020593. G-glutamylP_reductase_CS.
    IPR001057. Glu/AcGlu_kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR005766. P5_carboxy_syn.
    [Graphical view]
    PfamiPF00696. AA_kinase. 1 hit.
    PF00171. Aldedh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036429. P5C_syn. 1 hit.
    PRINTSiPR00474. GLU5KINASE.
    SUPFAMiSSF53633. SSF53633. 1 hit.
    SSF53720. SSF53720. 1 hit.
    TIGRFAMsiTIGR01092. P5CS. 1 hit.
    TIGR00407. proA. 1 hit.
    TIGR01027. proB. 1 hit.
    PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS01223. PROA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O65361-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDATRAFVKD VKRVVVKVGT AVVTRSDGRL ALGRLGSLCE QLKELNSDGY    50
    EVILVTSGAV SAGRQRLRFR KLVNSSFADL QKPQVELDGK ACAAVGQNGL 100
    MALYDTLFSQ LDLTAAQLLV TDNDFRDPSF RTQLTETVYQ LLDLKVVPVL 150
    NENDAVSTRK APYEDSSGIF WDNDSLAALL ALELKADLLI LLSDVDGLYN 200
    GPPSDPRSKL ISTYVKEKHQ GEITFGDKSR LGRGGMTAKV KAAVYAAYAG 250
    IPVIIASGKA TDNIIKVIDG QCVGTLFHKD AHLWVQVKET GVRDMAVAAR 300
    ESSRRLQAVS SEERKKILLD IADALEANEE KILAENEADV AAAQYAGYDR 350
    SLVARLAMNP DKISSLAKSI RVLADMEEPI GRILKRTEIA DGLILEKTSC 400
    PLGVLLIVFE SRPDALVQIA SLAIRSGNGL LLKGGKEAKR SNAILHKVIT 450
    SAIPDKVGEK LIGLVTSRDE IPDLLKLDDV IDLVIPRGSN KLVSQIKEST 500
    RIPVLGHADG ICHVYVDKSA NMDMAKRIVL DAKTDYPAAC NAMETLLVHK 550
    DLAENGGLND LIVDLRTEGV TMFGGPRIDA LQEFNIQATQ TFNREYSSPA 600
    CTVEIVDDVY AAIEHINHHG SAHTDCIIAE DHKVAETFLQ LVDSAAVLHN 650
    ASTRFCDGFR FGLGAEVGIS TSRIHARGPV GVEGLLTTRW VLKGSGQVVH 700
    GDKGVVYTHK DLPLVAQNS 719
    Length:719
    Mass (Da):77,855
    Last modified:August 1, 1998 - v1
    Checksum:i1510D11AF5559961
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF067967 mRNA. Translation: AAC18862.1.
    PIRiT12258.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF067967 mRNA. Translation: AAC18862.1 .
    PIRi T12258.

    3D structure databases

    ProteinModelPortali O65361.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00098 ; UER00359 .
    UPA00098 ; UER00360 .

    Family and domain databases

    Gene3Di 3.40.1160.10. 1 hit.
    3.40.309.10. 1 hit.
    3.40.605.10. 2 hits.
    HAMAPi MF_00412. ProA.
    MF_00456. ProB.
    InterProi IPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    IPR001048. Asp/Glu/Uridylate_kinase.
    IPR000965. G-glutamylP_reductase.
    IPR020593. G-glutamylP_reductase_CS.
    IPR001057. Glu/AcGlu_kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR005766. P5_carboxy_syn.
    [Graphical view ]
    Pfami PF00696. AA_kinase. 1 hit.
    PF00171. Aldedh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036429. P5C_syn. 1 hit.
    PRINTSi PR00474. GLU5KINASE.
    SUPFAMi SSF53633. SSF53633. 1 hit.
    SSF53720. SSF53720. 1 hit.
    TIGRFAMsi TIGR01092. P5CS. 1 hit.
    TIGR00407. proA. 1 hit.
    TIGR01027. proB. 1 hit.
    PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS01223. PROA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mesembryanthemum crystallinum pyrroline-5-carboxylate synthetase mRNA."
      Michalowski C.B., Quigley-Landreau F., Bohnert H.J.
      Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiP5CS_MESCR
    AccessioniPrimary (citable) accession number: O65361
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3