Reviewed, UniProtKB/Swiss-Prot O65361 (P5CS_MESCR)

Names and origin

Protein names

Recommended name:
    Delta-1-pyrroline-5-carboxylate synthetase
      Short name=P5CS
Including the following 2 domains:
    1- Recommended name:
            Glutamate 5-kinase
                Short name=GK
              EC=2.7.2.11
        Alternative name(s):
            Gamma-glutamyl kinase
    2- Recommended name:
            Gamma-glutamyl phosphate reductase
                Short name=GPR
              EC=1.2.1.41
        Alternative name(s):
            Glutamate-5-semialdehyde dehydrogenase
            Glutamyl-gamma-semialdehyde dehydrogenase

Gene names

Name:

P5CS

Organism

Mesembryanthemum crystallinum (Common ice plant)

Taxonomic identifier

3544 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Aizoaceae › Mesembryanthemum

Protein attributes

Sequence length	719 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH.
Enzyme regulation	Feedback regulated by proline.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.
Tissue specificity	Expressed at high levels in leaves and is inducible in roots subjected to salt stress.
Sequence similarities	In the N-terminal section; belongs to the glutamate 5-kinase family. In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Ligand	NADP
Molecular function	Kinase Oxidoreductase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 719	719	Delta-1-pyrroline-5-carboxylate synthetase		PRO_0000109776
Regions
	Region	1 – 293	293	Glutamate 5-kinase
	Region	294 – 719	426	Gamma-glutamyl phosphate reductase

Sequences

	Sequence		Length	Mass (Da)
	O65361-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 1510D11AF5559961	FASTA	719	77,855
	10 20 30 40 50 60 MDATRAFVKD VKRVVVKVGT AVVTRSDGRL ALGRLGSLCE QLKELNSDGY EVILVTSGAV 70 80 90 100 110 120 SAGRQRLRFR KLVNSSFADL QKPQVELDGK ACAAVGQNGL MALYDTLFSQ LDLTAAQLLV 130 140 150 160 170 180 TDNDFRDPSF RTQLTETVYQ LLDLKVVPVL NENDAVSTRK APYEDSSGIF WDNDSLAALL 190 200 210 220 230 240 ALELKADLLI LLSDVDGLYN GPPSDPRSKL ISTYVKEKHQ GEITFGDKSR LGRGGMTAKV 250 260 270 280 290 300 KAAVYAAYAG IPVIIASGKA TDNIIKVIDG QCVGTLFHKD AHLWVQVKET GVRDMAVAAR 310 320 330 340 350 360 ESSRRLQAVS SEERKKILLD IADALEANEE KILAENEADV AAAQYAGYDR SLVARLAMNP 370 380 390 400 410 420 DKISSLAKSI RVLADMEEPI GRILKRTEIA DGLILEKTSC PLGVLLIVFE SRPDALVQIA 430 440 450 460 470 480 SLAIRSGNGL LLKGGKEAKR SNAILHKVIT SAIPDKVGEK LIGLVTSRDE IPDLLKLDDV 490 500 510 520 530 540 IDLVIPRGSN KLVSQIKEST RIPVLGHADG ICHVYVDKSA NMDMAKRIVL DAKTDYPAAC 550 560 570 580 590 600 NAMETLLVHK DLAENGGLND LIVDLRTEGV TMFGGPRIDA LQEFNIQATQ TFNREYSSPA 610 620 630 640 650 660 CTVEIVDDVY AAIEHINHHG SAHTDCIIAE DHKVAETFLQ LVDSAAVLHN ASTRFCDGFR 670 680 690 700 710 FGLGAEVGIS TSRIHARGPV GVEGLLTTRW VLKGSGQVVH GDKGVVYTHK DLPLVAQNS

References

[1]	"Mesembryanthemum crystallinum pyrroline-5-carboxylate synthetase mRNA." Michalowski C.B., Quigley-Landreau F., Bohnert H.J. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF067967 mRNA. Translation: AAC18862.1.
PIR	T12258.
3D structure databases
HSSP	HSSP built from PDB template 1O20 based on UniProtKB Q9WYC9.
ModBase	Search...
Family and domain databases
InterPro	IPR016163. Ald_DHase_C. IPR016162. Ald_DHase_N. IPR001048. Asp/Glu/Uridylate_kinase. IPR000965. Gglut_pp_reduct. IPR001057. Glu_5kinase. IPR005766. P5_carboxy_syn. IPR005715. ProB. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF036429. P5C_syn. 1 hit.
PRINTS	PR00474. GLU5KINASE.
TIGRFAMs	TIGR01092. P5CS. 1 hit. TIGR00407. proA. 1 hit. TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P5CS_MESCR

Accession

Primary (citable) accession number: O65361

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	November 25, 2008
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)