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Protein

Delta-1-pyrroline-5-carboxylate synthase

Gene

P5CS

Organism
Mesembryanthemum crystallinum (Common ice plant) (Cryophytum crystallinum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.
L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH.

Enzyme regulationi

Feedback regulated by proline.

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 and 2 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Delta-1-pyrroline-5-carboxylate synthase (P5CS)
  2. Delta-1-pyrroline-5-carboxylate synthase (P5CS)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei57SubstrateBy similarity1
Binding sitei154SubstrateBy similarity1
Binding sitei173Substrate; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi193 – 194ATPBy similarity2
Nucleotide bindingi233 – 239ATPBy similarity7

GO - Molecular functioni

GO - Biological processi

Keywords - Molecular functioni

Kinase, Oxidoreductase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
UPA00098; UER00360.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-1-pyrroline-5-carboxylate synthase
Short name:
P5CS
Including the following 2 domains:
Glutamate 5-kinase (EC:2.7.2.11)
Short name:
GK
Alternative name(s):
Gamma-glutamyl kinase
Gamma-glutamyl phosphate reductase (EC:1.2.1.41)
Short name:
GPR
Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Gene namesi
Name:P5CS
OrganismiMesembryanthemum crystallinum (Common ice plant) (Cryophytum crystallinum)
Taxonomic identifieri3544 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAizoaceaeMesembryanthemumCryophytum

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001097761 – 719Delta-1-pyrroline-5-carboxylate synthaseAdd BLAST719

Expressioni

Tissue specificityi

Expressed at high levels in leaves and is inducible in roots subjected to salt stress.

Structurei

3D structure databases

ProteinModelPortaliO65361.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 293Glutamate 5-kinaseAdd BLAST293
Regioni294 – 719Gamma-glutamyl phosphate reductaseAdd BLAST426

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate 5-kinase family.Curated
In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.Curated

Family and domain databases

CDDicd07079. ALDH_F18-19_ProA-GPR. 1 hit.
Gene3Di3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPiMF_00412. ProA. 1 hit.
MF_00456. ProB. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR000965. GPR_dom.
IPR005766. P5_carboxy_syn.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF036429. P5C_syn. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O65361-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDATRAFVKD VKRVVVKVGT AVVTRSDGRL ALGRLGSLCE QLKELNSDGY
60 70 80 90 100
EVILVTSGAV SAGRQRLRFR KLVNSSFADL QKPQVELDGK ACAAVGQNGL
110 120 130 140 150
MALYDTLFSQ LDLTAAQLLV TDNDFRDPSF RTQLTETVYQ LLDLKVVPVL
160 170 180 190 200
NENDAVSTRK APYEDSSGIF WDNDSLAALL ALELKADLLI LLSDVDGLYN
210 220 230 240 250
GPPSDPRSKL ISTYVKEKHQ GEITFGDKSR LGRGGMTAKV KAAVYAAYAG
260 270 280 290 300
IPVIIASGKA TDNIIKVIDG QCVGTLFHKD AHLWVQVKET GVRDMAVAAR
310 320 330 340 350
ESSRRLQAVS SEERKKILLD IADALEANEE KILAENEADV AAAQYAGYDR
360 370 380 390 400
SLVARLAMNP DKISSLAKSI RVLADMEEPI GRILKRTEIA DGLILEKTSC
410 420 430 440 450
PLGVLLIVFE SRPDALVQIA SLAIRSGNGL LLKGGKEAKR SNAILHKVIT
460 470 480 490 500
SAIPDKVGEK LIGLVTSRDE IPDLLKLDDV IDLVIPRGSN KLVSQIKEST
510 520 530 540 550
RIPVLGHADG ICHVYVDKSA NMDMAKRIVL DAKTDYPAAC NAMETLLVHK
560 570 580 590 600
DLAENGGLND LIVDLRTEGV TMFGGPRIDA LQEFNIQATQ TFNREYSSPA
610 620 630 640 650
CTVEIVDDVY AAIEHINHHG SAHTDCIIAE DHKVAETFLQ LVDSAAVLHN
660 670 680 690 700
ASTRFCDGFR FGLGAEVGIS TSRIHARGPV GVEGLLTTRW VLKGSGQVVH
710
GDKGVVYTHK DLPLVAQNS
Length:719
Mass (Da):77,855
Last modified:August 1, 1998 - v1
Checksum:i1510D11AF5559961
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067967 mRNA. Translation: AAC18862.1.
PIRiT12258.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067967 mRNA. Translation: AAC18862.1.
PIRiT12258.

3D structure databases

ProteinModelPortaliO65361.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
UPA00098; UER00360.

Family and domain databases

CDDicd07079. ALDH_F18-19_ProA-GPR. 1 hit.
Gene3Di3.40.1160.10. 1 hit.
3.40.309.10. 1 hit.
3.40.605.10. 2 hits.
HAMAPiMF_00412. ProA. 1 hit.
MF_00456. ProB. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR020593. G-glutamylP_reductase_CS.
IPR001057. Glu/AcGlu_kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR000965. GPR_dom.
IPR005766. P5_carboxy_syn.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF00171. Aldedh. 1 hit.
[Graphical view]
PIRSFiPIRSF036429. P5C_syn. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01092. P5CS. 1 hit.
TIGR00407. proA. 1 hit.
TIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS01223. PROA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP5CS_MESCR
AccessioniPrimary (citable) accession number: O65361
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: October 5, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.