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Protein

Subtilisin-like protease SBT1.7

Gene

SBT1.7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine protease. Has a substrate preference for the hydrophobic residues Phe and Ala and the basic residue Asp in the P1 position, and for Asp, Leu or Ala in the P1' position (PubMed:12413398). Essential for mucilage release from seed coats. Triggers the accumulation and/or activation of cell wall modifying enzymes necessary either for the loosening of the outer primary cell wall, or to facilitate swelling of the mucilage (PubMed:18266922).2 Publications

Enzyme regulationi

Activated by calcium. Inhibited by the serine protease inhibitors 4-(2-aminoethyl)benzenesulphonyl fluoride (AEBSF), PMSF, di-isopropyl phosphofluoridate (DFP) and soybean trypsin inhibitor (SBTI). Not inhibited by benzamidine or iodoacetamide. Leupeptin and pepstatin A have a minor inhibitory action.1 Publication

pH dependencei

Optimum pH is 5.0.1 Publication

Temperature dependencei

Optimum temperature is 80 degrees Celsius. Thermostable.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei139Charge relay systemPROSITE-ProRule annotationBy similarity1
Active sitei212Charge relay systemPROSITE-ProRule annotationBy similarity1
Active sitei542Charge relay systemPROSITE-ProRule annotationBy similarity1

GO - Molecular functioni

  • serine-type endopeptidase activity Source: TAIR

GO - Biological processi

  • mucilage extrusion from seed coat Source: TAIR
  • mucilage metabolic process involved in seed coat development Source: TAIR
  • seed coat development Source: TAIR

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
LigandCalcium

Protein family/group databases

MEROPSiS08.112

Names & Taxonomyi

Protein namesi
Recommended name:
Subtilisin-like protease SBT1.71 Publication (EC:3.4.21.-Curated)
Alternative name(s):
Cucumisin-like serine protease
Subtilase subfamily 1 member 71 Publication
Short name:
AtSBT1.71 Publication
Subtilisin-like serine protease 11 Publication
Short name:
At-SLP11 Publication
Gene namesi
Name:SBT1.71 Publication
Synonyms:ARA12, ASP481 Publication, SLP11 Publication
Ordered Locus Names:At5g67360
ORF Names:K8K14.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G67360
TAIRilocus:2158187 AT5G67360

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant seeds are defective in mucilage extrusion.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000004284625 – 106Sequence analysis2 PublicationsAdd BLAST82
ChainiPRO_0000042847107 – 757Subtilisin-like protease SBT1.71 PublicationAdd BLAST651

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi170N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi352N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi376N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi379N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi631N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi644N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Zymogen

Proteomic databases

PaxDbiO65351
PRIDEiO65351

Expressioni

Tissue specificityi

Expressed in immature siliques and at lower levels in stems and flowers (PubMed:11055401, PubMed:7647567, PubMed:12413398). Widely expressed at low levels (PubMed:18266922).4 Publications

Developmental stagei

Highest levels of expression detected during silique development (PubMed:7647567). Hihghly expressed in the seed coat during seed development (PubMed:18266922).2 Publications

Inductioni

By methyl jasmonate.1 Publication

Gene expression databases

ExpressionAtlasiO65351 baseline and differential
GenevisibleiO65351 AT

Interactioni

Protein-protein interaction databases

BioGridi22113, 2 interactors
STRINGi3702.AT5G67360.1

Structurei

3D structure databases

ProteinModelPortaliO65351
SMRiO65351
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini131 – 579Peptidase S8Add BLAST449

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IEMN Eukaryota
COG1404 LUCA
HOGENOMiHOG000238262
InParanoidiO65351
OMAiNTVNGPD
OrthoDBiEOG093603BF
PhylomeDBiO65351

Family and domain databases

CDDicd04852 Peptidases_S8_3, 1 hit
Gene3Di3.30.70.80, 1 hit
3.40.50.200, 2 hits
InterProiView protein in InterPro
IPR003137 PA_domain
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR034197 Peptidases_S8_3
IPR010259 S8pro/Inhibitor_I9
IPR037045 S8pro/Inhibitor_I9_sf
PfamiView protein in Pfam
PF05922 Inhibitor_I9, 1 hit
PF02225 PA, 1 hit
PF00082 Peptidase_S8, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF52743 SSF52743, 2 hits
PROSITEiView protein in PROSITE
PS00138 SUBTILASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O65351-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSFLSSTA FFLLLCLGFC HVSSSSSDQG TYIVHMAKSQ MPSSFDLHSN
60 70 80 90 100
WYDSSLRSIS DSAELLYTYE NAIHGFSTRL TQEEADSLMT QPGVISVLPE
110 120 130 140 150
HRYELHTTRT PLFLGLDEHT ADLFPEAGSY SDVVVGVLDT GVWPESKSYS
160 170 180 190 200
DEGFGPIPSS WKGGCEAGTN FTASLCNRKL IGARFFARGY ESTMGPIDES
210 220 230 240 250
KESRSPRDDD GHGTHTSSTA AGSVVEGASL LGYASGTARG MAPRARVAVY
260 270 280 290 300
KVCWLGGCFS SDILAAIDKA IADNVNVLSM SLGGGMSDYY RDGVAIGAFA
310 320 330 340 350
AMERGILVSC SAGNAGPSSS SLSNVAPWIT TVGAGTLDRD FPALAILGNG
360 370 380 390 400
KNFTGVSLFK GEALPDKLLP FIYAGNASNA TNGNLCMTGT LIPEKVKGKI
410 420 430 440 450
VMCDRGINAR VQKGDVVKAA GGVGMILANT AANGEELVAD AHLLPATTVG
460 470 480 490 500
EKAGDIIRHY VTTDPNPTAS ISILGTVVGV KPSPVVAAFS SRGPNSITPN
510 520 530 540 550
ILKPDLIAPG VNILAAWTGA AGPTGLASDS RRVEFNIISG TSMSCPHVSG
560 570 580 590 600
LAALLKSVHP EWSPAAIRSA LMTTAYKTYK DGKPLLDIAT GKPSTPFDHG
610 620 630 640 650
AGHVSPTTAT NPGLIYDLTT EDYLGFLCAL NYTSPQIRSV SRRNYTCDPS
660 670 680 690 700
KSYSVADLNY PSFAVNVDGV GAYKYTRTVT SVGGAGTYSV KVTSETTGVK
710 720 730 740 750
ISVEPAVLNF KEANEKKSYT VTFTVDSSKP SGSNSFGSIE WSDGKHVVGS

PVAISWT
Length:757
Mass (Da):79,415
Last modified:August 1, 1998 - v1
Checksum:iE7F68FCAD16700AB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41M → T in CAA59963 (PubMed:7647567).Curated1
Sequence conflicti242 – 247APRARV → LHAL in CAA59963 (PubMed:7647567).Curated6
Sequence conflicti562W → C in AAM10321 (PubMed:14593172).Curated1
Sequence conflicti562W → C in AAN46863 (PubMed:14593172).Curated1
Sequence conflicti670V → A in CAA59963 (PubMed:7647567).Curated1

Mass spectrometryi

Molecular mass is 76102.8 Da from positions 107 - 757. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF065639 Genomic DNA Translation: AAC18851.1
AB007645 Genomic DNA Translation: BAB09021.1
CP002688 Genomic DNA Translation: AED98332.1
AF360285 mRNA Translation: AAK25995.1
AY091773 mRNA Translation: AAM10321.1
AY142612 mRNA Translation: AAN13181.1
BT001082 mRNA Translation: AAN46863.1
X85974 mRNA Translation: CAA59963.1
PIRiJC7519
S52770
RefSeqiNP_569048.1, NM_126136.3
UniGeneiAt.23238
At.67722
At.71531

Genome annotation databases

EnsemblPlantsiAT5G67360.1; AT5G67360.1; AT5G67360
GeneIDi836871
GrameneiAT5G67360.1; AT5G67360.1; AT5G67360
KEGGiath:AT5G67360

Similar proteinsi

Entry informationi

Entry nameiSBT17_ARATH
AccessioniPrimary (citable) accession number: O65351
Secondary accession number(s): P80854, Q39007, Q8RWQ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health