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Protein

Subtilisin-like protease SBT1.7

Gene

SBT1.7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease. Has a substrate preference for the hydrophobic residues Phe and Ala and the basic residue Asp in the P1 position, and for Asp, Leu or Ala in the P1' position (PubMed:12413398). Essential for mucilage release from seed coats. Triggers the accumulation and/or activation of cell wall modifying enzymes necessary either for the loosening of the outer primary cell wall, or to facilitate swelling of the mucilage (PubMed:18266922).2 Publications

Enzyme regulationi

Activated by calcium. Inhibited by the serine protease inhibitors 4-(2-aminoethyl)benzenesulphonyl fluoride (AEBSF), PMSF, di-isopropyl phosphofluoridate (DFP) and soybean trypsin inhibitor (SBTI). Not inhibited by benzamidine or iodoacetamide. Leupeptin and pepstatin A have a minor inhibitory action.1 Publication

pH dependencei

Optimum pH is 5.0.1 Publication

Temperature dependencei

Optimum temperature is 80 degrees Celsius. Thermostable.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei139 – 1391Charge relay systemPROSITE-ProRule annotationBy similarity
Active sitei212 – 2121Charge relay systemPROSITE-ProRule annotationBy similarity
Active sitei542 – 5421Charge relay systemPROSITE-ProRule annotationBy similarity

GO - Molecular functioni

  • serine-type endopeptidase activity Source: TAIR

GO - Biological processi

  • mucilage extrusion from seed coat Source: TAIR
  • mucilage metabolic process involved in seed coat development Source: TAIR
  • seed coat development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

Calcium

Enzyme and pathway databases

BioCyciARA:AT5G67360-MONOMER.

Protein family/group databases

MEROPSiS08.112.

Names & Taxonomyi

Protein namesi
Recommended name:
Subtilisin-like protease SBT1.71 Publication (EC:3.4.21.-Curated)
Alternative name(s):
Cucumisin-like serine protease
Subtilase subfamily 1 member 71 Publication
Short name:
AtSBT1.71 Publication
Subtilisin-like serine protease 11 Publication
Short name:
At-SLP11 Publication
Gene namesi
Name:SBT1.71 Publication
Synonyms:ARA12, ASP481 Publication, SLP11 Publication
Ordered Locus Names:At5g67360
ORF Names:K8K14.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G67360.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • cell wall Source: TAIR
  • extracellular region Source: TAIR
  • plant-type cell wall Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant seeds are defective in mucilage extrusion.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Propeptidei25 – 10682Sequence analysis2 PublicationsPRO_0000042846Add
BLAST
Chaini107 – 757651Subtilisin-like protease SBT1.71 PublicationPRO_0000042847Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence analysis
Glycosylationi352 – 3521N-linked (GlcNAc...)Sequence analysis
Glycosylationi376 – 3761N-linked (GlcNAc...)Sequence analysis
Glycosylationi379 – 3791N-linked (GlcNAc...)Sequence analysis
Glycosylationi631 – 6311N-linked (GlcNAc...)Sequence analysis
Glycosylationi644 – 6441N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein, Zymogen

Proteomic databases

PaxDbiO65351.
PRIDEiO65351.

Expressioni

Tissue specificityi

Expressed in immature siliques and at lower levels in stems and flowers (PubMed:11055401, PubMed:7647567, PubMed:12413398). Widely expressed at low levels (PubMed:18266922).4 Publications

Developmental stagei

Highest levels of expression detected during silique development (PubMed:7647567). Hihghly expressed in the seed coat during seed development (PubMed:18266922).2 Publications

Inductioni

By methyl jasmonate.1 Publication

Gene expression databases

GenevisibleiO65351. AT.

Interactioni

Protein-protein interaction databases

BioGridi22113. 2 interactions.
STRINGi3702.AT5G67360.1.

Structurei

3D structure databases

ProteinModelPortaliO65351.
SMRiO65351. Positions 194-339, 477-588.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini131 – 579449Peptidase S8Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IEMN. Eukaryota.
COG1404. LUCA.
HOGENOMiHOG000238262.
InParanoidiO65351.
OMAiAGHIDAT.
OrthoDBiEOG093603BF.
PhylomeDBiO65351.

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 3 hits.
InterProiIPR003137. PA_domain.
IPR000209. Peptidase_S8/S53_dom.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF05922. Inhibitor_I9. 1 hit.
PF02225. PA. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 2 hits.
PROSITEiPS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O65351-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSFLSSTA FFLLLCLGFC HVSSSSSDQG TYIVHMAKSQ MPSSFDLHSN
60 70 80 90 100
WYDSSLRSIS DSAELLYTYE NAIHGFSTRL TQEEADSLMT QPGVISVLPE
110 120 130 140 150
HRYELHTTRT PLFLGLDEHT ADLFPEAGSY SDVVVGVLDT GVWPESKSYS
160 170 180 190 200
DEGFGPIPSS WKGGCEAGTN FTASLCNRKL IGARFFARGY ESTMGPIDES
210 220 230 240 250
KESRSPRDDD GHGTHTSSTA AGSVVEGASL LGYASGTARG MAPRARVAVY
260 270 280 290 300
KVCWLGGCFS SDILAAIDKA IADNVNVLSM SLGGGMSDYY RDGVAIGAFA
310 320 330 340 350
AMERGILVSC SAGNAGPSSS SLSNVAPWIT TVGAGTLDRD FPALAILGNG
360 370 380 390 400
KNFTGVSLFK GEALPDKLLP FIYAGNASNA TNGNLCMTGT LIPEKVKGKI
410 420 430 440 450
VMCDRGINAR VQKGDVVKAA GGVGMILANT AANGEELVAD AHLLPATTVG
460 470 480 490 500
EKAGDIIRHY VTTDPNPTAS ISILGTVVGV KPSPVVAAFS SRGPNSITPN
510 520 530 540 550
ILKPDLIAPG VNILAAWTGA AGPTGLASDS RRVEFNIISG TSMSCPHVSG
560 570 580 590 600
LAALLKSVHP EWSPAAIRSA LMTTAYKTYK DGKPLLDIAT GKPSTPFDHG
610 620 630 640 650
AGHVSPTTAT NPGLIYDLTT EDYLGFLCAL NYTSPQIRSV SRRNYTCDPS
660 670 680 690 700
KSYSVADLNY PSFAVNVDGV GAYKYTRTVT SVGGAGTYSV KVTSETTGVK
710 720 730 740 750
ISVEPAVLNF KEANEKKSYT VTFTVDSSKP SGSNSFGSIE WSDGKHVVGS

PVAISWT
Length:757
Mass (Da):79,415
Last modified:August 1, 1998 - v1
Checksum:iE7F68FCAD16700AB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti41 – 411M → T in CAA59963 (PubMed:7647567).Curated
Sequence conflicti242 – 2476APRARV → LHAL in CAA59963 (PubMed:7647567).Curated
Sequence conflicti562 – 5621W → C in AAM10321 (PubMed:14593172).Curated
Sequence conflicti562 – 5621W → C in AAN46863 (PubMed:14593172).Curated
Sequence conflicti670 – 6701V → A in CAA59963 (PubMed:7647567).Curated

Mass spectrometryi

Molecular mass is 76102.8 Da from positions 107 - 757. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF065639 Genomic DNA. Translation: AAC18851.1.
AB007645 Genomic DNA. Translation: BAB09021.1.
CP002688 Genomic DNA. Translation: AED98332.1.
AF360285 mRNA. Translation: AAK25995.1.
AY091773 mRNA. Translation: AAM10321.1.
AY142612 mRNA. Translation: AAN13181.1.
BT001082 mRNA. Translation: AAN46863.1.
X85974 mRNA. Translation: CAA59963.1.
PIRiJC7519.
S52770.
RefSeqiNP_569048.1. NM_126136.2.
UniGeneiAt.23238.
At.67722.
At.71531.

Genome annotation databases

EnsemblPlantsiAT5G67360.1; AT5G67360.1; AT5G67360.
GeneIDi836871.
GrameneiAT5G67360.1; AT5G67360.1; AT5G67360.
KEGGiath:AT5G67360.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF065639 Genomic DNA. Translation: AAC18851.1.
AB007645 Genomic DNA. Translation: BAB09021.1.
CP002688 Genomic DNA. Translation: AED98332.1.
AF360285 mRNA. Translation: AAK25995.1.
AY091773 mRNA. Translation: AAM10321.1.
AY142612 mRNA. Translation: AAN13181.1.
BT001082 mRNA. Translation: AAN46863.1.
X85974 mRNA. Translation: CAA59963.1.
PIRiJC7519.
S52770.
RefSeqiNP_569048.1. NM_126136.2.
UniGeneiAt.23238.
At.67722.
At.71531.

3D structure databases

ProteinModelPortaliO65351.
SMRiO65351. Positions 194-339, 477-588.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22113. 2 interactions.
STRINGi3702.AT5G67360.1.

Protein family/group databases

MEROPSiS08.112.

Proteomic databases

PaxDbiO65351.
PRIDEiO65351.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G67360.1; AT5G67360.1; AT5G67360.
GeneIDi836871.
GrameneiAT5G67360.1; AT5G67360.1; AT5G67360.
KEGGiath:AT5G67360.

Organism-specific databases

TAIRiAT5G67360.

Phylogenomic databases

eggNOGiENOG410IEMN. Eukaryota.
COG1404. LUCA.
HOGENOMiHOG000238262.
InParanoidiO65351.
OMAiAGHIDAT.
OrthoDBiEOG093603BF.
PhylomeDBiO65351.

Enzyme and pathway databases

BioCyciARA:AT5G67360-MONOMER.

Miscellaneous databases

PROiO65351.

Gene expression databases

GenevisibleiO65351. AT.

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 3 hits.
InterProiIPR003137. PA_domain.
IPR000209. Peptidase_S8/S53_dom.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF05922. Inhibitor_I9. 1 hit.
PF02225. PA. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 2 hits.
PROSITEiPS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSBT17_ARATH
AccessioniPrimary (citable) accession number: O65351
Secondary accession number(s): P80854, Q39007, Q8RWQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.