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O65312 (MEDEA_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histone-lysine N-methyltransferase MEDEA

EC=2.1.1.43
Alternative name(s):
Maternal embryogenesis control protein
Protein EMBRYO DEFECTIVE 173
Protein FERTILIZATION-INDEPENDENT SEED 1
Protein SET DOMAIN GROUP 5
Gene names
Name:MEA
Synonyms:EMB173, FIS1, MEDEA, SDG5, SET5
Ordered Locus Names:At1g02580
ORF Names:T14P4.11
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length689 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Polycomb group (PcG) protein. Catalytic subunit of some PcG multiprotein complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target genes. Required to prevent the proliferation of the central cell of the female gametophyte by repressing target genes before fertilization. After fertilization, it probably also regulates the embryo and endosperm proliferation and anteroposterior organization during seed development. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. Interacts with the promoter and repress the transcription of genes such as PHE1 and PHE2, that are paternally active and maternally silenced genes. Ref.1 Ref.6 Ref.10 Ref.12 Ref.13 Ref.16

Catalytic activity

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

Subunit structure

Interacts directly with FIE via its N-terminal domain. These two proteins are probably inderectly associated with FIS2. In plants, PcG complexes are probably composed of a member of the EZ family (CLF or MEA), FIE, and a member of the VEFS family (FIS2, VRN2 or EMF2). Interacts with TAF13. Ref.7 Ref.8 Ref.9 Ref.17

Subcellular location

Nucleus. Note: Excluded from the nucleolus. Ref.7

Tissue specificity

Expressed in unpollinated siliques that contain maturing gametophytes. Not expressed at early stages of floral development during early megagametogenesis. Ref.1 Ref.7 Ref.8

Developmental stage

Expressed both maternally and zygotically. Expressed in both egg and central cell before fertilization. After fertilization, it is expressed in the embryo and endosperm, then decreases during seed maturation.

Induction

Maternal MEA allele is activated by DME but repressed by MET1 in the central cell of the female gametophyte, the progenitor of the endosperm. Ref.11 Ref.14

Miscellaneous

The MEA locus is imprinted. Maternal inherited gene is expressed in the ovule (the egg and the central cell), while the paternal inherited gene is silenced in the pollen. After fertilization, only the maternal inherited allele is expressed. The paternal repression is dependent on DDM1 protein, which may methylate the paternal locus, while the maternal inherited allele is allowed by the DME protein, which may antagonize or suppress DDM1 dependent methylation, and activates its transcription.

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. EZ subfamily.

Contains 1 CXC domain.

Contains 1 SANT domain.

Contains 1 SET domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   LigandS-adenosyl-L-methionine
   Molecular functionDevelopmental protein
Methyltransferase
Repressor
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processnegative regulation of transcription, DNA-templated

Inferred from mutant phenotype PubMed 16651654. Source: TAIR

regulation of endosperm development

Inferred from mutant phenotype Ref.6. Source: TAIR

regulation of gene expression by genetic imprinting

Inferred from mutant phenotype PubMed 19783734. Source: TAIR

response to absence of light

Inferred from expression pattern PubMed 12376626. Source: TAIR

seed morphogenesis

Inferred from genetic interaction PubMed 19783734. Source: TAIR

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentPcG protein complex

Inferred from electronic annotation. Source: InterPro

nucleus

Inferred from direct assay Ref.8. Source: TAIR

   Molecular_functionchromatin binding

Inferred from electronic annotation. Source: InterPro

histone-lysine N-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

protein binding

Inferred from physical interaction Ref.8Ref.7Ref.9PubMed 12970192PubMed 15456723PubMed 15469499PubMed 18812497. Source: IntAct

sequence-specific DNA binding

Inferred from direct assay PubMed 16651654. Source: TAIR

sequence-specific DNA binding transcription factor activity

Inferred from sequence or structural similarity PubMed 11118137. Source: TAIR

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

FIEQ9LT4712EBI-632832,EBI-307146

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 689689Histone-lysine N-methyltransferase MEDEA
PRO_0000213997

Regions

Domain339 – 38951SANT
Domain428 – 532105CXC
Domain544 – 659116SET
Region1 – 109109Interaction with FIE
Compositional bias450 – 51970Cys-rich

Sequences

Sequence LengthMass (Da)Tools
O65312 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 2103D06ACD589CDF

FASTA68979,310
        10         20         30         40         50         60 
MEKENHEDDG EGLPPELNQI KEQIEKERFL HIKRKFELRY IPSVATHASH HQSFDLNQPA 

        70         80         90        100        110        120 
AEDDNGGDNK SLLSRMQNPL RHFSASSDYN SYEDQGYVLD EDQDYALEED VPLFLDEDVP 

       130        140        150        160        170        180 
LLPSVKLPIV EKLPRSITWV FTKSSQLMAE SDSVIGKRQI YYLNGEALEL SSEEDEEDEE 

       190        200        210        220        230        240 
EDEEEIKKEK CEFSEDVDRF IWTVGQDYGL DDLVVRRALA KYLEVDVSDI LERYNELKLK 

       250        260        270        280        290        300 
NDGTAGEASD LTSKTITTAF QDFADRRHCR RCMIFDCHMH EKYEPESRSS EDKSSLFEDE 

       310        320        330        340        350        360 
DRQPCSEHCY LKVRSVTEAD HVMDNDNSIS NKIVVSDPNN TMWTPVEKDL YLKGIEIFGR 

       370        380        390        400        410        420 
NSCDVALNIL RGLKTCLEIY NYMREQDQCT MSLDLNKTTQ RHNQVTKKVS RKSSRSVRKK 

       430        440        450        460        470        480 
SRLRKYARYP PALKKTTSGE AKFYKHYTPC TCKSKCGQQC PCLTHENCCE KYCGCSKDCN 

       490        500        510        520        530        540 
NRFGGCNCAI GQCTNRQCPC FAANRECDPD LCRSCPLSCG DGTLGETPVQ IQCKNMQFLL 

       550        560        570        580        590        600 
QTNKKILIGK SDVHGWGAFT WDSLKKNEYL GEYTGELITH DEANERGRIE DRIGSSYLFT 

       610        620        630        640        650        660 
LNDQLEIDAR RKGNEFKFLN HSARPNCYAK LMIVRGDQRI GLFAERAIEE GEELFFDYCY 

       670        680 
GPEHADWSRG REPRKTGASK RSKEARPAR 

« Hide

References

« Hide 'large scale' references
[1]"Maternal control of embryogenesis by MEDEA, a polycomb group gene in Arabidopsis."
Grossniklaus U., Vielle-Calzada J.-P., Hoeppner M.A., Gagliano W.B.
Science 280:446-450(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Strain: cv. Landsberg erecta.
Tissue: Flower bud.
[2]"Genes controlling fertilization-independent seed development in Arabidopsis thaliana."
Luo M., Bilodeau P., Koltunow A., Dennis E.S., Peacock W.J., Chaudhury A.
Proc. Natl. Acad. Sci. U.S.A. 96:296-301(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Landsberg erecta.
Tissue: Silique.
[3]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Maintenance of genomic imprinting at the Arabidopsis medea locus requires zygotic DDM1 activity."
Vielle-Calzada J.-P., Thomas J., Spillane C., Coluccio A., Hoeppner M.A., Grossniklaus U.
Genes Dev. 13:2971-2982(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: IMPRINTING.
[6]"Control of fertilization-independent endosperm development by the MEDEA polycomb gene in Arabidopsis."
Kiyosue T., Ohad N., Yadegari R., Hannon M., Dinneny J., Wells D., Katz A., Margossian L., Harada J.J., Goldberg R.B., Fischer R.L.
Proc. Natl. Acad. Sci. U.S.A. 96:4186-4191(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Interaction of the Arabidopsis polycomb group proteins FIE and MEA mediates their common phenotypes."
Spillane C., MacDougall C., Stock C., Koehler C., Vielle-Calzada J.-P., Nunes S.M., Grossniklaus U., Goodrich J.
Curr. Biol. 10:1535-1538(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH FIE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[8]"Expression and parent-of-origin effects for FIS2, MEA, and FIE in the endosperm and embryo of developing Arabidopsis seeds."
Luo M., Bilodeau P., Dennis E.S., Peacock W.J., Chaudhury A.
Proc. Natl. Acad. Sci. U.S.A. 97:10637-10642(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH FIE, TISSUE SPECIFICITY.
[9]"Mutations in the FIE and MEA genes that encode interacting polycomb proteins cause parent-of-origin effects on seed development by distinct mechanisms."
Yadegari R., Kinoshita T., Lotan O., Cohen G., Katz A., Choi Y., Katz A., Nakashima K., Harada J.J., Goldberg R.B., Fischer R.L., Ohad N.
Plant Cell 12:2367-2382(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH FIE.
[10]"Polycomb group genes control pattern formation in plant seed."
Soerensen M.B., Chaudhury A.M., Robert H., Bancharel E., Berger F.
Curr. Biol. 11:277-281(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"Imprinting of the MEA Polycomb gene is controlled by antagonism between MET1 methyltransferase and DME glycosylase."
Xiao W., Gehring M., Choi Y., Margossian L., Pu H., Harada J.J., Goldberg R.B., Pennell R.I., Fischer R.L.
Dev. Cell 5:891-901(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[12]"The Polycomb-group protein MEDEA regulates seed development by controlling expression of the MADS-box gene PHERES1."
Koehler C., Hennig L., Spillane C., Pien S., Gruissem W., Grossniklaus U.
Genes Dev. 17:1540-1553(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"Identification of new members of fertilisation independent seed Polycomb group pathway involved in the control of seed development in Arabidopsis thaliana."
Guitton A.-E., Page D.R., Chambrier P., Lionnet C., Faure J.-E., Grossniklaus U., Berger F.
Development 131:2971-2981(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[14]"An invariant aspartic acid in the DNA glycosylase domain of DEMETER is necessary for transcriptional activation of the imprinted MEDEA gene."
Choi Y., Harada J.J., Goldberg R.B., Fischer R.L.
Proc. Natl. Acad. Sci. U.S.A. 101:7481-7486(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: REGULATION OF IMPRINTING BY DME.
[15]"Genomic imprinting in plants: the epigenetic version of an Oedipus complex."
Autran D., Huanca-Mamani W., Vielle-Calzada J.-P.
Curr. Opin. Plant Biol. 8:19-25(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[16]"The Arabidopsis thaliana MEDEA Polycomb group protein controls expression of PHERES1 by parental imprinting."
Koehler C., Page D.R., Gagliardini V., Grossniklaus U.
Nat. Genet. 37:28-30(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[17]"TAF13 interacts with PRC2 members and is essential for Arabidopsis seed development."
Lindner M., Simonini S., Kooiker M., Gagliardini V., Somssich M., Hohenstatt M., Simon R., Grossniklaus U., Kater M.M.
Dev. Biol. 379:28-37(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TAF13.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF060485 mRNA. Translation: AAC39446.1.
AF096094 Genomic DNA. Translation: AAD09103.1.
AC022521 Genomic DNA. Translation: AAG10636.1.
CP002684 Genomic DNA. Translation: AEE27447.1.
PIRT52060.
RefSeqNP_563658.1. NM_100139.3.
UniGeneAt.10786.

3D structure databases

ProteinModelPortalO65312.
SMRO65312. Positions 449-660.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid24657. 5 interactions.
IntActO65312. 4 interactions.

Proteomic databases

PaxDbO65312.
PRIDEO65312.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G02580.1; AT1G02580.1; AT1G02580.
GeneID839422.
KEGGath:AT1G02580.

Organism-specific databases

GeneFarm2217.
TAIRAT1G02580.

Phylogenomic databases

eggNOGCOG2940.
HOGENOMHOG000083511.
InParanoidO65312.
KOK11430.
OMASARPNCY.
PhylomeDBO65312.

Enzyme and pathway databases

BioCycARA:AT1G02580-MONOMER.

Gene expression databases

GenevestigatorO65312.

Family and domain databases

InterProIPR026489. CXC_dom.
IPR025778. Hist-Lys_N-MeTrfase_EZ.
IPR001005. SANT/Myb.
IPR001214. SET_dom.
[Graphical view]
PfamPF00856. SET. 1 hit.
[Graphical view]
SMARTSM00717. SANT. 1 hit.
SM00317. SET. 1 hit.
[Graphical view]
PROSITEPS51633. CXC. 1 hit.
PS51576. SAM_MT43_EZ. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMEDEA_ARATH
AccessionPrimary (citable) accession number: O65312
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: August 1, 1998
Last modified: June 11, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names