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Protein

Histone-lysine N-methyltransferase MEDEA

Gene

MEA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Polycomb group (PcG) protein. Catalytic subunit of some PcG multiprotein complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target genes. Required to prevent the proliferation of the central cell of the female gametophyte by repressing target genes before fertilization. After fertilization, it probably also regulates the embryo and endosperm proliferation and anteroposterior organization during seed development. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. Interacts with the promoter and repress the transcription of genes such as PHE1 and PHE2, that are paternally active and maternally silenced genes.6 Publications

Miscellaneous

The MEA locus is imprinted. Maternal inherited gene is expressed in the ovule (the egg and the central cell), while the paternal inherited gene is silenced in the pollen. After fertilization, only the maternal inherited allele is expressed. The paternal repression is dependent on DDM1 protein, which may methylate the paternal locus, while the maternal inherited allele is allowed by the DME protein, which may antagonize or suppress DDM1 dependent methylation, and activates its transcription.

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N6-methyl-L-lysine-[histone].PROSITE-ProRule annotation

GO - Molecular functioni

  • DNA binding transcription factor activity Source: TAIR
  • histone-lysine N-methyltransferase activity Source: UniProtKB-EC
  • sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • negative regulation of transcription, DNA-templated Source: TAIR
  • regulation of endosperm development Source: TAIR
  • regulation of gene expression by genetic imprinting Source: TAIR
  • response to absence of light Source: TAIR
  • seed morphogenesis Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, Methyltransferase, Repressor, Transferase
Biological processTranscription, Transcription regulation
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-ATH-212300 PRC2 methylates histones and DNA
R-ATH-2559580 Oxidative Stress Induced Senescence
R-ATH-8953750 Transcriptional Regulation by E2F6

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase MEDEA (EC:2.1.1.43)
Alternative name(s):
Maternal embryogenesis control protein
Protein EMBRYO DEFECTIVE 173
Protein FERTILIZATION-INDEPENDENT SEED 1
Protein SET DOMAIN GROUP 5
Gene namesi
Name:MEA
Synonyms:EMB173, FIS1, MEDEA, SDG5, SET5
Ordered Locus Names:At1g02580
ORF Names:T14P4.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G02580
TAIRilocus:2196110 AT1G02580

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002139971 – 689Histone-lysine N-methyltransferase MEDEAAdd BLAST689

Proteomic databases

PaxDbiO65312

Expressioni

Tissue specificityi

Expressed in unpollinated siliques that contain maturing gametophytes. Not expressed at early stages of floral development during early megagametogenesis.3 Publications

Developmental stagei

Expressed both maternally and zygotically. Expressed in both egg and central cell before fertilization. After fertilization, it is expressed in the embryo and endosperm, then decreases during seed maturation.

Inductioni

Maternal MEA allele is activated by DME but repressed by MET1 in the central cell of the female gametophyte, the progenitor of the endosperm.1 Publication

Gene expression databases

ExpressionAtlasiO65312 baseline and differential
GenevisibleiO65312 AT

Interactioni

Subunit structurei

Interacts directly with FIE via its N-terminal domain. These two proteins are probably inderectly associated with FIS2. In plants, PcG complexes are probably composed of a member of the EZ family (CLF or MEA), FIE, and a member of the VEFS family (FIS2, VRN2 or EMF2). Interacts with TAF13.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
FIEQ9LT4712EBI-632832,EBI-307146

Protein-protein interaction databases

BioGridi24657, 5 interactors
IntActiO65312, 4 interactors
STRINGi3702.AT1G02580.1

Structurei

3D structure databases

ProteinModelPortaliO65312
SMRiO65312
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini339 – 389SANTAdd BLAST51
Domaini428 – 532CXCPROSITE-ProRule annotationAdd BLAST105
Domaini544 – 659SETPROSITE-ProRule annotationAdd BLAST116

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 109Interaction with FIEAdd BLAST109

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi450 – 519Cys-richAdd BLAST70

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. EZ subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1079 Eukaryota
COG2940 LUCA
HOGENOMiHOG000083511
KOiK11430
OMAiHFNENDD
OrthoDBiEOG09360260
PhylomeDBiO65312

Family and domain databases

InterProiView protein in InterPro
IPR026489 CXC_dom
IPR025778 Hist-Lys_N-MeTrfase_EZ
IPR001214 SET_dom
IPR033467 Tesmin/TSO1-like_CXC
PANTHERiPTHR22884:SF237 PTHR22884:SF237, 2 hits
PfamiView protein in Pfam
PF00856 SET, 1 hit
SMARTiView protein in SMART
SM01114 CXC, 1 hit
SM00317 SET, 1 hit
PROSITEiView protein in PROSITE
PS51633 CXC, 1 hit
PS51576 SAM_MT43_EZ, 1 hit
PS50280 SET, 1 hit

Sequencei

Sequence statusi: Complete.

O65312-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKENHEDDG EGLPPELNQI KEQIEKERFL HIKRKFELRY IPSVATHASH
60 70 80 90 100
HQSFDLNQPA AEDDNGGDNK SLLSRMQNPL RHFSASSDYN SYEDQGYVLD
110 120 130 140 150
EDQDYALEED VPLFLDEDVP LLPSVKLPIV EKLPRSITWV FTKSSQLMAE
160 170 180 190 200
SDSVIGKRQI YYLNGEALEL SSEEDEEDEE EDEEEIKKEK CEFSEDVDRF
210 220 230 240 250
IWTVGQDYGL DDLVVRRALA KYLEVDVSDI LERYNELKLK NDGTAGEASD
260 270 280 290 300
LTSKTITTAF QDFADRRHCR RCMIFDCHMH EKYEPESRSS EDKSSLFEDE
310 320 330 340 350
DRQPCSEHCY LKVRSVTEAD HVMDNDNSIS NKIVVSDPNN TMWTPVEKDL
360 370 380 390 400
YLKGIEIFGR NSCDVALNIL RGLKTCLEIY NYMREQDQCT MSLDLNKTTQ
410 420 430 440 450
RHNQVTKKVS RKSSRSVRKK SRLRKYARYP PALKKTTSGE AKFYKHYTPC
460 470 480 490 500
TCKSKCGQQC PCLTHENCCE KYCGCSKDCN NRFGGCNCAI GQCTNRQCPC
510 520 530 540 550
FAANRECDPD LCRSCPLSCG DGTLGETPVQ IQCKNMQFLL QTNKKILIGK
560 570 580 590 600
SDVHGWGAFT WDSLKKNEYL GEYTGELITH DEANERGRIE DRIGSSYLFT
610 620 630 640 650
LNDQLEIDAR RKGNEFKFLN HSARPNCYAK LMIVRGDQRI GLFAERAIEE
660 670 680
GEELFFDYCY GPEHADWSRG REPRKTGASK RSKEARPAR
Length:689
Mass (Da):79,310
Last modified:August 1, 1998 - v1
Checksum:i2103D06ACD589CDF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF060485 mRNA Translation: AAC39446.1
AF096094 Genomic DNA Translation: AAD09103.1
AC022521 Genomic DNA Translation: AAG10636.1
CP002684 Genomic DNA Translation: AEE27447.1
PIRiT52060
RefSeqiNP_563658.1, NM_100139.4
UniGeneiAt.10786

Genome annotation databases

EnsemblPlantsiAT1G02580.1; AT1G02580.1; AT1G02580
GeneIDi839422
GrameneiAT1G02580.1; AT1G02580.1; AT1G02580
KEGGiath:AT1G02580

Similar proteinsi

Entry informationi

Entry nameiMEDEA_ARATH
AccessioniPrimary (citable) accession number: O65312
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health