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O65312

- MEDEA_ARATH

UniProt

O65312 - MEDEA_ARATH

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Protein

Histone-lysine N-methyltransferase MEDEA

Gene

MEA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Polycomb group (PcG) protein. Catalytic subunit of some PcG multiprotein complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target genes. Required to prevent the proliferation of the central cell of the female gametophyte by repressing target genes before fertilization. After fertilization, it probably also regulates the embryo and endosperm proliferation and anteroposterior organization during seed development. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. Interacts with the promoter and repress the transcription of genes such as PHE1 and PHE2, that are paternally active and maternally silenced genes.6 Publications

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].PROSITE-ProRule annotation

GO - Molecular functioni

  1. chromatin binding Source: InterPro
  2. histone-lysine N-methyltransferase activity Source: UniProtKB-EC
  3. sequence-specific DNA binding Source: TAIR
  4. sequence-specific DNA binding transcription factor activity Source: TAIR

GO - Biological processi

  1. negative regulation of transcription, DNA-templated Source: TAIR
  2. regulation of endosperm development Source: TAIR
  3. regulation of gene expression by genetic imprinting Source: TAIR
  4. response to absence of light Source: TAIR
  5. seed morphogenesis Source: TAIR
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Methyltransferase, Repressor, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARA:AT1G02580-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase MEDEA (EC:2.1.1.43)
Alternative name(s):
Maternal embryogenesis control protein
Protein EMBRYO DEFECTIVE 173
Protein FERTILIZATION-INDEPENDENT SEED 1
Protein SET DOMAIN GROUP 5
Gene namesi
Name:MEA
Synonyms:EMB173, FIS1, MEDEA, SDG5, SET5
Ordered Locus Names:At1g02580
ORF Names:T14P4.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G02580.

Subcellular locationi

Nucleus 1 Publication
Note: Excluded from the nucleolus.

GO - Cellular componenti

  1. nucleus Source: TAIR
  2. PcG protein complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 689689Histone-lysine N-methyltransferase MEDEAPRO_0000213997Add
BLAST

Proteomic databases

PaxDbiO65312.
PRIDEiO65312.

Expressioni

Tissue specificityi

Expressed in unpollinated siliques that contain maturing gametophytes. Not expressed at early stages of floral development during early megagametogenesis.3 Publications

Developmental stagei

Expressed both maternally and zygotically. Expressed in both egg and central cell before fertilization. After fertilization, it is expressed in the embryo and endosperm, then decreases during seed maturation.

Inductioni

Maternal MEA allele is activated by DME but repressed by MET1 in the central cell of the female gametophyte, the progenitor of the endosperm.1 Publication

Gene expression databases

ExpressionAtlasiO65312. differential.
GenevestigatoriO65312.

Interactioni

Subunit structurei

Interacts directly with FIE via its N-terminal domain. These two proteins are probably inderectly associated with FIS2. In plants, PcG complexes are probably composed of a member of the EZ family (CLF or MEA), FIE, and a member of the VEFS family (FIS2, VRN2 or EMF2). Interacts with TAF13.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
FIEQ9LT4712EBI-632832,EBI-307146

Protein-protein interaction databases

BioGridi24657. 5 interactions.
IntActiO65312. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliO65312.
SMRiO65312. Positions 449-660.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini339 – 38951SANTAdd
BLAST
Domaini428 – 532105CXCPROSITE-ProRule annotationAdd
BLAST
Domaini544 – 659116SETPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 109109Interaction with FIEAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi450 – 51970Cys-richAdd
BLAST

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. EZ subfamily.PROSITE-ProRule annotation
Contains 1 CXC domain.PROSITE-ProRule annotation
Contains 1 SANT domain.Curated
Contains 1 SET domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG2940.
HOGENOMiHOG000083511.
KOiK11430.
OMAiSARPNCY.
PhylomeDBiO65312.

Family and domain databases

InterProiIPR026489. CXC_dom.
IPR025778. Hist-Lys_N-MeTrfase_EZ.
IPR001005. SANT/Myb.
IPR001214. SET_dom.
[Graphical view]
PfamiPF00856. SET. 1 hit.
[Graphical view]
SMARTiSM00717. SANT. 1 hit.
SM00317. SET. 1 hit.
[Graphical view]
PROSITEiPS51633. CXC. 1 hit.
PS51576. SAM_MT43_EZ. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O65312-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEKENHEDDG EGLPPELNQI KEQIEKERFL HIKRKFELRY IPSVATHASH
60 70 80 90 100
HQSFDLNQPA AEDDNGGDNK SLLSRMQNPL RHFSASSDYN SYEDQGYVLD
110 120 130 140 150
EDQDYALEED VPLFLDEDVP LLPSVKLPIV EKLPRSITWV FTKSSQLMAE
160 170 180 190 200
SDSVIGKRQI YYLNGEALEL SSEEDEEDEE EDEEEIKKEK CEFSEDVDRF
210 220 230 240 250
IWTVGQDYGL DDLVVRRALA KYLEVDVSDI LERYNELKLK NDGTAGEASD
260 270 280 290 300
LTSKTITTAF QDFADRRHCR RCMIFDCHMH EKYEPESRSS EDKSSLFEDE
310 320 330 340 350
DRQPCSEHCY LKVRSVTEAD HVMDNDNSIS NKIVVSDPNN TMWTPVEKDL
360 370 380 390 400
YLKGIEIFGR NSCDVALNIL RGLKTCLEIY NYMREQDQCT MSLDLNKTTQ
410 420 430 440 450
RHNQVTKKVS RKSSRSVRKK SRLRKYARYP PALKKTTSGE AKFYKHYTPC
460 470 480 490 500
TCKSKCGQQC PCLTHENCCE KYCGCSKDCN NRFGGCNCAI GQCTNRQCPC
510 520 530 540 550
FAANRECDPD LCRSCPLSCG DGTLGETPVQ IQCKNMQFLL QTNKKILIGK
560 570 580 590 600
SDVHGWGAFT WDSLKKNEYL GEYTGELITH DEANERGRIE DRIGSSYLFT
610 620 630 640 650
LNDQLEIDAR RKGNEFKFLN HSARPNCYAK LMIVRGDQRI GLFAERAIEE
660 670 680
GEELFFDYCY GPEHADWSRG REPRKTGASK RSKEARPAR
Length:689
Mass (Da):79,310
Last modified:August 1, 1998 - v1
Checksum:i2103D06ACD589CDF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF060485 mRNA. Translation: AAC39446.1.
AF096094 Genomic DNA. Translation: AAD09103.1.
AC022521 Genomic DNA. Translation: AAG10636.1.
CP002684 Genomic DNA. Translation: AEE27447.1.
PIRiT52060.
RefSeqiNP_563658.1. NM_100139.3.
UniGeneiAt.10786.

Genome annotation databases

EnsemblPlantsiAT1G02580.1; AT1G02580.1; AT1G02580.
GeneIDi839422.
KEGGiath:AT1G02580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF060485 mRNA. Translation: AAC39446.1 .
AF096094 Genomic DNA. Translation: AAD09103.1 .
AC022521 Genomic DNA. Translation: AAG10636.1 .
CP002684 Genomic DNA. Translation: AEE27447.1 .
PIRi T52060.
RefSeqi NP_563658.1. NM_100139.3.
UniGenei At.10786.

3D structure databases

ProteinModelPortali O65312.
SMRi O65312. Positions 449-660.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 24657. 5 interactions.
IntActi O65312. 4 interactions.

Proteomic databases

PaxDbi O65312.
PRIDEi O65312.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G02580.1 ; AT1G02580.1 ; AT1G02580 .
GeneIDi 839422.
KEGGi ath:AT1G02580.

Organism-specific databases

GeneFarmi 2217.
TAIRi AT1G02580.

Phylogenomic databases

eggNOGi COG2940.
HOGENOMi HOG000083511.
KOi K11430.
OMAi SARPNCY.
PhylomeDBi O65312.

Enzyme and pathway databases

BioCyci ARA:AT1G02580-MONOMER.

Gene expression databases

ExpressionAtlasi O65312. differential.
Genevestigatori O65312.

Family and domain databases

InterProi IPR026489. CXC_dom.
IPR025778. Hist-Lys_N-MeTrfase_EZ.
IPR001005. SANT/Myb.
IPR001214. SET_dom.
[Graphical view ]
Pfami PF00856. SET. 1 hit.
[Graphical view ]
SMARTi SM00717. SANT. 1 hit.
SM00317. SET. 1 hit.
[Graphical view ]
PROSITEi PS51633. CXC. 1 hit.
PS51576. SAM_MT43_EZ. 1 hit.
PS50280. SET. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Maternal control of embryogenesis by MEDEA, a polycomb group gene in Arabidopsis."
    Grossniklaus U., Vielle-Calzada J.-P., Hoeppner M.A., Gagliano W.B.
    Science 280:446-450(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: cv. Landsberg erecta.
    Tissue: Flower bud.
  2. "Genes controlling fertilization-independent seed development in Arabidopsis thaliana."
    Luo M., Bilodeau P., Koltunow A., Dennis E.S., Peacock W.J., Chaudhury A.
    Proc. Natl. Acad. Sci. U.S.A. 96:296-301(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Landsberg erecta.
    Tissue: Silique.
  3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Maintenance of genomic imprinting at the Arabidopsis medea locus requires zygotic DDM1 activity."
    Vielle-Calzada J.-P., Thomas J., Spillane C., Coluccio A., Hoeppner M.A., Grossniklaus U.
    Genes Dev. 13:2971-2982(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IMPRINTING.
  6. "Control of fertilization-independent endosperm development by the MEDEA polycomb gene in Arabidopsis."
    Kiyosue T., Ohad N., Yadegari R., Hannon M., Dinneny J., Wells D., Katz A., Margossian L., Harada J.J., Goldberg R.B., Fischer R.L.
    Proc. Natl. Acad. Sci. U.S.A. 96:4186-4191(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Interaction of the Arabidopsis polycomb group proteins FIE and MEA mediates their common phenotypes."
    Spillane C., MacDougall C., Stock C., Koehler C., Vielle-Calzada J.-P., Nunes S.M., Grossniklaus U., Goodrich J.
    Curr. Biol. 10:1535-1538(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FIE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  8. "Expression and parent-of-origin effects for FIS2, MEA, and FIE in the endosperm and embryo of developing Arabidopsis seeds."
    Luo M., Bilodeau P., Dennis E.S., Peacock W.J., Chaudhury A.
    Proc. Natl. Acad. Sci. U.S.A. 97:10637-10642(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FIE, TISSUE SPECIFICITY.
  9. "Mutations in the FIE and MEA genes that encode interacting polycomb proteins cause parent-of-origin effects on seed development by distinct mechanisms."
    Yadegari R., Kinoshita T., Lotan O., Cohen G., Katz A., Choi Y., Katz A., Nakashima K., Harada J.J., Goldberg R.B., Fischer R.L., Ohad N.
    Plant Cell 12:2367-2382(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FIE.
  10. "Polycomb group genes control pattern formation in plant seed."
    Soerensen M.B., Chaudhury A.M., Robert H., Bancharel E., Berger F.
    Curr. Biol. 11:277-281(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Imprinting of the MEA Polycomb gene is controlled by antagonism between MET1 methyltransferase and DME glycosylase."
    Xiao W., Gehring M., Choi Y., Margossian L., Pu H., Harada J.J., Goldberg R.B., Pennell R.I., Fischer R.L.
    Dev. Cell 5:891-901(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  12. "The Polycomb-group protein MEDEA regulates seed development by controlling expression of the MADS-box gene PHERES1."
    Koehler C., Hennig L., Spillane C., Pien S., Gruissem W., Grossniklaus U.
    Genes Dev. 17:1540-1553(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Identification of new members of fertilisation independent seed Polycomb group pathway involved in the control of seed development in Arabidopsis thaliana."
    Guitton A.-E., Page D.R., Chambrier P., Lionnet C., Faure J.-E., Grossniklaus U., Berger F.
    Development 131:2971-2981(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "An invariant aspartic acid in the DNA glycosylase domain of DEMETER is necessary for transcriptional activation of the imprinted MEDEA gene."
    Choi Y., Harada J.J., Goldberg R.B., Fischer R.L.
    Proc. Natl. Acad. Sci. U.S.A. 101:7481-7486(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: REGULATION OF IMPRINTING BY DME.
  15. "Genomic imprinting in plants: the epigenetic version of an Oedipus complex."
    Autran D., Huanca-Mamani W., Vielle-Calzada J.-P.
    Curr. Opin. Plant Biol. 8:19-25(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  16. "The Arabidopsis thaliana MEDEA Polycomb group protein controls expression of PHERES1 by parental imprinting."
    Koehler C., Page D.R., Gagliardini V., Grossniklaus U.
    Nat. Genet. 37:28-30(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  17. "TAF13 interacts with PRC2 members and is essential for Arabidopsis seed development."
    Lindner M., Simonini S., Kooiker M., Gagliardini V., Somssich M., Hohenstatt M., Simon R., Grossniklaus U., Kater M.M.
    Dev. Biol. 379:28-37(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TAF13.

Entry informationi

Entry nameiMEDEA_ARATH
AccessioniPrimary (citable) accession number: O65312
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The MEA locus is imprinted. Maternal inherited gene is expressed in the ovule (the egg and the central cell), while the paternal inherited gene is silenced in the pollen. After fertilization, only the maternal inherited allele is expressed. The paternal repression is dependent on DDM1 protein, which may methylate the paternal locus, while the maternal inherited allele is allowed by the DME protein, which may antagonize or suppress DDM1 dependent methylation, and activates its transcription.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3