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O65312

- MEDEA_ARATH

UniProt

O65312 - MEDEA_ARATH

Protein

Histone-lysine N-methyltransferase MEDEA

Gene

MEA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Polycomb group (PcG) protein. Catalytic subunit of some PcG multiprotein complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target genes. Required to prevent the proliferation of the central cell of the female gametophyte by repressing target genes before fertilization. After fertilization, it probably also regulates the embryo and endosperm proliferation and anteroposterior organization during seed development. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. Interacts with the promoter and repress the transcription of genes such as PHE1 and PHE2, that are paternally active and maternally silenced genes.6 Publications

    Catalytic activityi

    S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].PROSITE-ProRule annotation

    GO - Molecular functioni

    1. chromatin binding Source: InterPro
    2. histone-lysine N-methyltransferase activity Source: UniProtKB-EC
    3. protein binding Source: IntAct
    4. sequence-specific DNA binding Source: TAIR
    5. sequence-specific DNA binding transcription factor activity Source: TAIR

    GO - Biological processi

    1. negative regulation of transcription, DNA-templated Source: TAIR
    2. regulation of endosperm development Source: TAIR
    3. regulation of gene expression by genetic imprinting Source: TAIR
    4. response to absence of light Source: TAIR
    5. seed morphogenesis Source: TAIR
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Methyltransferase, Repressor, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciARA:AT1G02580-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histone-lysine N-methyltransferase MEDEA (EC:2.1.1.43)
    Alternative name(s):
    Maternal embryogenesis control protein
    Protein EMBRYO DEFECTIVE 173
    Protein FERTILIZATION-INDEPENDENT SEED 1
    Protein SET DOMAIN GROUP 5
    Gene namesi
    Name:MEA
    Synonyms:EMB173, FIS1, MEDEA, SDG5, SET5
    Ordered Locus Names:At1g02580
    ORF Names:T14P4.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G02580.

    Subcellular locationi

    Nucleus 1 Publication
    Note: Excluded from the nucleolus.

    GO - Cellular componenti

    1. nucleus Source: TAIR
    2. PcG protein complex Source: InterPro

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 689689Histone-lysine N-methyltransferase MEDEAPRO_0000213997Add
    BLAST

    Proteomic databases

    PaxDbiO65312.
    PRIDEiO65312.

    Expressioni

    Tissue specificityi

    Expressed in unpollinated siliques that contain maturing gametophytes. Not expressed at early stages of floral development during early megagametogenesis.3 Publications

    Developmental stagei

    Expressed both maternally and zygotically. Expressed in both egg and central cell before fertilization. After fertilization, it is expressed in the embryo and endosperm, then decreases during seed maturation.

    Inductioni

    Maternal MEA allele is activated by DME but repressed by MET1 in the central cell of the female gametophyte, the progenitor of the endosperm.1 Publication

    Gene expression databases

    GenevestigatoriO65312.

    Interactioni

    Subunit structurei

    Interacts directly with FIE via its N-terminal domain. These two proteins are probably inderectly associated with FIS2. In plants, PcG complexes are probably composed of a member of the EZ family (CLF or MEA), FIE, and a member of the VEFS family (FIS2, VRN2 or EMF2). Interacts with TAF13.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    FIEQ9LT4712EBI-632832,EBI-307146

    Protein-protein interaction databases

    BioGridi24657. 5 interactions.
    IntActiO65312. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliO65312.
    SMRiO65312. Positions 449-660.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini339 – 38951SANTAdd
    BLAST
    Domaini428 – 532105CXCPROSITE-ProRule annotationAdd
    BLAST
    Domaini544 – 659116SETPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 109109Interaction with FIEAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi450 – 51970Cys-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. EZ subfamily.PROSITE-ProRule annotation
    Contains 1 CXC domain.PROSITE-ProRule annotation
    Contains 1 SANT domain.Curated
    Contains 1 SET domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2940.
    HOGENOMiHOG000083511.
    InParanoidiO65312.
    KOiK11430.
    OMAiSARPNCY.
    PhylomeDBiO65312.

    Family and domain databases

    InterProiIPR026489. CXC_dom.
    IPR025778. Hist-Lys_N-MeTrfase_EZ.
    IPR001005. SANT/Myb.
    IPR001214. SET_dom.
    [Graphical view]
    PfamiPF00856. SET. 1 hit.
    [Graphical view]
    SMARTiSM00717. SANT. 1 hit.
    SM00317. SET. 1 hit.
    [Graphical view]
    PROSITEiPS51633. CXC. 1 hit.
    PS51576. SAM_MT43_EZ. 1 hit.
    PS50280. SET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O65312-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKENHEDDG EGLPPELNQI KEQIEKERFL HIKRKFELRY IPSVATHASH    50
    HQSFDLNQPA AEDDNGGDNK SLLSRMQNPL RHFSASSDYN SYEDQGYVLD 100
    EDQDYALEED VPLFLDEDVP LLPSVKLPIV EKLPRSITWV FTKSSQLMAE 150
    SDSVIGKRQI YYLNGEALEL SSEEDEEDEE EDEEEIKKEK CEFSEDVDRF 200
    IWTVGQDYGL DDLVVRRALA KYLEVDVSDI LERYNELKLK NDGTAGEASD 250
    LTSKTITTAF QDFADRRHCR RCMIFDCHMH EKYEPESRSS EDKSSLFEDE 300
    DRQPCSEHCY LKVRSVTEAD HVMDNDNSIS NKIVVSDPNN TMWTPVEKDL 350
    YLKGIEIFGR NSCDVALNIL RGLKTCLEIY NYMREQDQCT MSLDLNKTTQ 400
    RHNQVTKKVS RKSSRSVRKK SRLRKYARYP PALKKTTSGE AKFYKHYTPC 450
    TCKSKCGQQC PCLTHENCCE KYCGCSKDCN NRFGGCNCAI GQCTNRQCPC 500
    FAANRECDPD LCRSCPLSCG DGTLGETPVQ IQCKNMQFLL QTNKKILIGK 550
    SDVHGWGAFT WDSLKKNEYL GEYTGELITH DEANERGRIE DRIGSSYLFT 600
    LNDQLEIDAR RKGNEFKFLN HSARPNCYAK LMIVRGDQRI GLFAERAIEE 650
    GEELFFDYCY GPEHADWSRG REPRKTGASK RSKEARPAR 689
    Length:689
    Mass (Da):79,310
    Last modified:August 1, 1998 - v1
    Checksum:i2103D06ACD589CDF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF060485 mRNA. Translation: AAC39446.1.
    AF096094 Genomic DNA. Translation: AAD09103.1.
    AC022521 Genomic DNA. Translation: AAG10636.1.
    CP002684 Genomic DNA. Translation: AEE27447.1.
    PIRiT52060.
    RefSeqiNP_563658.1. NM_100139.3.
    UniGeneiAt.10786.

    Genome annotation databases

    EnsemblPlantsiAT1G02580.1; AT1G02580.1; AT1G02580.
    GeneIDi839422.
    KEGGiath:AT1G02580.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF060485 mRNA. Translation: AAC39446.1 .
    AF096094 Genomic DNA. Translation: AAD09103.1 .
    AC022521 Genomic DNA. Translation: AAG10636.1 .
    CP002684 Genomic DNA. Translation: AEE27447.1 .
    PIRi T52060.
    RefSeqi NP_563658.1. NM_100139.3.
    UniGenei At.10786.

    3D structure databases

    ProteinModelPortali O65312.
    SMRi O65312. Positions 449-660.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 24657. 5 interactions.
    IntActi O65312. 4 interactions.

    Proteomic databases

    PaxDbi O65312.
    PRIDEi O65312.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G02580.1 ; AT1G02580.1 ; AT1G02580 .
    GeneIDi 839422.
    KEGGi ath:AT1G02580.

    Organism-specific databases

    GeneFarmi 2217.
    TAIRi AT1G02580.

    Phylogenomic databases

    eggNOGi COG2940.
    HOGENOMi HOG000083511.
    InParanoidi O65312.
    KOi K11430.
    OMAi SARPNCY.
    PhylomeDBi O65312.

    Enzyme and pathway databases

    BioCyci ARA:AT1G02580-MONOMER.

    Gene expression databases

    Genevestigatori O65312.

    Family and domain databases

    InterProi IPR026489. CXC_dom.
    IPR025778. Hist-Lys_N-MeTrfase_EZ.
    IPR001005. SANT/Myb.
    IPR001214. SET_dom.
    [Graphical view ]
    Pfami PF00856. SET. 1 hit.
    [Graphical view ]
    SMARTi SM00717. SANT. 1 hit.
    SM00317. SET. 1 hit.
    [Graphical view ]
    PROSITEi PS51633. CXC. 1 hit.
    PS51576. SAM_MT43_EZ. 1 hit.
    PS50280. SET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Maternal control of embryogenesis by MEDEA, a polycomb group gene in Arabidopsis."
      Grossniklaus U., Vielle-Calzada J.-P., Hoeppner M.A., Gagliano W.B.
      Science 280:446-450(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
      Strain: cv. Landsberg erecta.
      Tissue: Flower bud.
    2. "Genes controlling fertilization-independent seed development in Arabidopsis thaliana."
      Luo M., Bilodeau P., Koltunow A., Dennis E.S., Peacock W.J., Chaudhury A.
      Proc. Natl. Acad. Sci. U.S.A. 96:296-301(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Landsberg erecta.
      Tissue: Silique.
    3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Maintenance of genomic imprinting at the Arabidopsis medea locus requires zygotic DDM1 activity."
      Vielle-Calzada J.-P., Thomas J., Spillane C., Coluccio A., Hoeppner M.A., Grossniklaus U.
      Genes Dev. 13:2971-2982(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IMPRINTING.
    6. "Control of fertilization-independent endosperm development by the MEDEA polycomb gene in Arabidopsis."
      Kiyosue T., Ohad N., Yadegari R., Hannon M., Dinneny J., Wells D., Katz A., Margossian L., Harada J.J., Goldberg R.B., Fischer R.L.
      Proc. Natl. Acad. Sci. U.S.A. 96:4186-4191(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Interaction of the Arabidopsis polycomb group proteins FIE and MEA mediates their common phenotypes."
      Spillane C., MacDougall C., Stock C., Koehler C., Vielle-Calzada J.-P., Nunes S.M., Grossniklaus U., Goodrich J.
      Curr. Biol. 10:1535-1538(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FIE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    8. "Expression and parent-of-origin effects for FIS2, MEA, and FIE in the endosperm and embryo of developing Arabidopsis seeds."
      Luo M., Bilodeau P., Dennis E.S., Peacock W.J., Chaudhury A.
      Proc. Natl. Acad. Sci. U.S.A. 97:10637-10642(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FIE, TISSUE SPECIFICITY.
    9. "Mutations in the FIE and MEA genes that encode interacting polycomb proteins cause parent-of-origin effects on seed development by distinct mechanisms."
      Yadegari R., Kinoshita T., Lotan O., Cohen G., Katz A., Choi Y., Katz A., Nakashima K., Harada J.J., Goldberg R.B., Fischer R.L., Ohad N.
      Plant Cell 12:2367-2382(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FIE.
    10. "Polycomb group genes control pattern formation in plant seed."
      Soerensen M.B., Chaudhury A.M., Robert H., Bancharel E., Berger F.
      Curr. Biol. 11:277-281(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Imprinting of the MEA Polycomb gene is controlled by antagonism between MET1 methyltransferase and DME glycosylase."
      Xiao W., Gehring M., Choi Y., Margossian L., Pu H., Harada J.J., Goldberg R.B., Pennell R.I., Fischer R.L.
      Dev. Cell 5:891-901(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    12. "The Polycomb-group protein MEDEA regulates seed development by controlling expression of the MADS-box gene PHERES1."
      Koehler C., Hennig L., Spillane C., Pien S., Gruissem W., Grossniklaus U.
      Genes Dev. 17:1540-1553(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    13. "Identification of new members of fertilisation independent seed Polycomb group pathway involved in the control of seed development in Arabidopsis thaliana."
      Guitton A.-E., Page D.R., Chambrier P., Lionnet C., Faure J.-E., Grossniklaus U., Berger F.
      Development 131:2971-2981(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    14. "An invariant aspartic acid in the DNA glycosylase domain of DEMETER is necessary for transcriptional activation of the imprinted MEDEA gene."
      Choi Y., Harada J.J., Goldberg R.B., Fischer R.L.
      Proc. Natl. Acad. Sci. U.S.A. 101:7481-7486(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: REGULATION OF IMPRINTING BY DME.
    15. "Genomic imprinting in plants: the epigenetic version of an Oedipus complex."
      Autran D., Huanca-Mamani W., Vielle-Calzada J.-P.
      Curr. Opin. Plant Biol. 8:19-25(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    16. "The Arabidopsis thaliana MEDEA Polycomb group protein controls expression of PHERES1 by parental imprinting."
      Koehler C., Page D.R., Gagliardini V., Grossniklaus U.
      Nat. Genet. 37:28-30(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    17. "TAF13 interacts with PRC2 members and is essential for Arabidopsis seed development."
      Lindner M., Simonini S., Kooiker M., Gagliardini V., Somssich M., Hohenstatt M., Simon R., Grossniklaus U., Kater M.M.
      Dev. Biol. 379:28-37(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TAF13.

    Entry informationi

    Entry nameiMEDEA_ARATH
    AccessioniPrimary (citable) accession number: O65312
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2003
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The MEA locus is imprinted. Maternal inherited gene is expressed in the ovule (the egg and the central cell), while the paternal inherited gene is silenced in the pollen. After fertilization, only the maternal inherited allele is expressed. The paternal repression is dependent on DDM1 protein, which may methylate the paternal locus, while the maternal inherited allele is allowed by the DME protein, which may antagonize or suppress DDM1 dependent methylation, and activates its transcription.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3