Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

20 kDa chaperonin, chloroplastic

Gene

CPN20

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seems to function only as a co-chaperone, along with CPN60, and in certain cases is essential for the discharge of biologically active proteins from CPN60 (PubMed:17178727, PubMed:23057508). Required to activate the iron superoxide dismutases (FeSOD) (PubMed:23057508).2 Publications
Involved in abscisic acid (ABA) signaling, independently of its co-chaperone role. Acts as negative regulator of the CHLH-WRKY40 coupled ABA signaling pathway, downstream of CHLH and upstream of WRKY40.1 Publication

GO - Molecular functioni

  • ATP binding Source: InterPro
  • chaperone binding Source: GO_Central
  • copper ion binding Source: TAIR
  • unfolded protein binding Source: GO_Central

GO - Biological processi

  • chaperone mediated protein folding requiring cofactor Source: GO_Central
  • positive regulation of superoxide dismutase activity Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to cold Source: TAIR
  • response to unfolded protein Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Names & Taxonomyi

Protein namesi
Recommended name:
20 kDa chaperonin, chloroplastic
Alternative name(s):
Chaperonin 10
Short name:
Ch-CPN10
Short name:
Cpn10
Chaperonin 20
Protein Cpn21
Gene namesi
Name:CPN20
Synonyms:CHCPN10, CPN21
Ordered Locus Names:At5g20720
ORF Names:T1M15.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G20720.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • chloroplast thylakoid membrane Source: TAIR
  • mitochondrial matrix Source: GO_Central
  • mitochondrion Source: TAIR
  • plastid Source: TAIR
  • thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi83G → A: Reduces co-chaperone activity more than 20-fold. 1 Publication1
Mutagenesisi86L → A: Reduces co-chaperone activity more than 20-fold. 1 Publication1
Mutagenesisi181G → A: Reduces co-chaperone activity more than 3-fold. 1 Publication1
Mutagenesisi184L → A: Reduces co-chaperone activity more than 20-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 50ChloroplastSequence analysisAdd BLAST50
ChainiPRO_000000504551 – 25320 kDa chaperonin, chloroplasticAdd BLAST203

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei212PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO65282.
PRIDEiO65282.

2D gel databases

SWISS-2DPAGEO65282.

PTM databases

iPTMnetiO65282.

Expressioni

Tissue specificityi

Ubiquitous. Most abundant in leaves and inflorescence. Low levels found in roots.1 Publication

Inductioni

Induced by heat treatment.1 Publication

Gene expression databases

GenevisibleiO65282. AT.

Interactioni

Subunit structurei

Homotetramer. Forms stable complexes with CPN60 in the presence of ATP (PubMed:10205903). Interacts with FSD1 (PubMed:23057508). Interacts with CLPT1 and CLPT2 (PubMed:25921872). Interacts with CHLH (PubMed:23783410).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
FSD1P212762EBI-4435709,EBI-4466816

GO - Molecular functioni

Protein-protein interaction databases

BioGridi17470. 3 interactors.
IntActiO65282. 5 interactors.
STRINGi3702.AT5G20720.1.

Structurei

3D structure databases

ProteinModelPortaliO65282.
SMRiO65282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni60 – 153Cpn-10 domain 1CuratedAdd BLAST94
Regioni159 – 252Cpn-10 domain 2CuratedAdd BLAST94

Sequence similaritiesi

Belongs to the GroES chaperonin family.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG1641. Eukaryota.
COG0234. LUCA.
HOGENOMiHOG000133897.
InParanoidiO65282.
KOiK04078.
OMAiQVTEMES.
OrthoDBiEOG09360K0I.
PhylomeDBiO65282.

Family and domain databases

CDDicd00320. cpn10. 2 hits.
Gene3Di2.30.33.40. 2 hits.
HAMAPiMF_00580. CH10. 2 hits.
InterProiIPR020818. Chaperonin_GroES.
IPR018369. Chaprnonin_Cpn10_CS.
IPR017416. Cpn20.
IPR011032. GroES-like.
[Graphical view]
PANTHERiPTHR10772. PTHR10772. 1 hit.
PfamiPF00166. Cpn10. 2 hits.
[Graphical view]
PIRSFiPIRSF038157. Chaperonin_21_chloroplast. 1 hit.
PRINTSiPR00297. CHAPERONIN10.
SMARTiSM00883. Cpn10. 2 hits.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
PROSITEiPS00681. CHAPERONINS_CPN10. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O65282-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATQLTASP VTMSARSLAS LDGLRASSVK FSSLKPGTLR QSQFRRLVVK
60 70 80 90 100
AASVVAPKYT SIKPLGDRVL VKIKEAEEKT LGGILLPSTA QSKPQGGEVV
110 120 130 140 150
AVGEGRTIGK NKIDITVPTG AQIIYSKYAG TEVEFNDVKH LILKEDDIVG
160 170 180 190 200
ILETEDIKDL KPLNDRVFIK VAEAEEKTAG GLLLTETTKE KPSIGTVIAV
210 220 230 240 250
GPGSLDEEGK ITPLPVSTGS TVLYSKYAGN DFKGKDGSNY IALRASDVMA

ILS
Length:253
Mass (Da):26,802
Last modified:December 8, 2000 - v2
Checksum:i43A8CD78E7C95BCC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31F → V in AAC14026 (Ref. 3) Curated1
Sequence conflicti86 – 87LP → TFH in AAC14026 (Ref. 3) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010818 mRNA. Translation: CAA09368.1.
AF268068 mRNA. Translation: AAG13931.1.
AF059037 mRNA. Translation: AAC14026.1.
AB007130 mRNA. Translation: BAB61619.1.
AF296832 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92881.1.
CP002688 Genomic DNA. Translation: AED92882.1.
CP002688 Genomic DNA. Translation: AED92883.1.
AY062971 mRNA. Translation: AAL33817.1.
AY034979 mRNA. Translation: AAK59484.1.
AF428366 mRNA. Translation: AAL16296.1.
AF428339 mRNA. Translation: AAL16269.1.
PIRiT52122.
T52613.
RefSeqiNP_197572.1. NM_122079.4.
NP_851045.1. NM_180714.4.
UniGeneiAt.49045.
At.59645.

Genome annotation databases

EnsemblPlantsiAT5G20720.1; AT5G20720.1; AT5G20720.
AT5G20720.2; AT5G20720.2; AT5G20720.
AT5G20720.3; AT5G20720.3; AT5G20720.
GeneIDi832195.
GrameneiAT5G20720.1; AT5G20720.1; AT5G20720.
AT5G20720.2; AT5G20720.2; AT5G20720.
AT5G20720.3; AT5G20720.3; AT5G20720.
KEGGiath:AT5G20720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010818 mRNA. Translation: CAA09368.1.
AF268068 mRNA. Translation: AAG13931.1.
AF059037 mRNA. Translation: AAC14026.1.
AB007130 mRNA. Translation: BAB61619.1.
AF296832 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92881.1.
CP002688 Genomic DNA. Translation: AED92882.1.
CP002688 Genomic DNA. Translation: AED92883.1.
AY062971 mRNA. Translation: AAL33817.1.
AY034979 mRNA. Translation: AAK59484.1.
AF428366 mRNA. Translation: AAL16296.1.
AF428339 mRNA. Translation: AAL16269.1.
PIRiT52122.
T52613.
RefSeqiNP_197572.1. NM_122079.4.
NP_851045.1. NM_180714.4.
UniGeneiAt.49045.
At.59645.

3D structure databases

ProteinModelPortaliO65282.
SMRiO65282.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17470. 3 interactors.
IntActiO65282. 5 interactors.
STRINGi3702.AT5G20720.1.

PTM databases

iPTMnetiO65282.

2D gel databases

SWISS-2DPAGEO65282.

Proteomic databases

PaxDbiO65282.
PRIDEiO65282.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G20720.1; AT5G20720.1; AT5G20720.
AT5G20720.2; AT5G20720.2; AT5G20720.
AT5G20720.3; AT5G20720.3; AT5G20720.
GeneIDi832195.
GrameneiAT5G20720.1; AT5G20720.1; AT5G20720.
AT5G20720.2; AT5G20720.2; AT5G20720.
AT5G20720.3; AT5G20720.3; AT5G20720.
KEGGiath:AT5G20720.

Organism-specific databases

TAIRiAT5G20720.

Phylogenomic databases

eggNOGiKOG1641. Eukaryota.
COG0234. LUCA.
HOGENOMiHOG000133897.
InParanoidiO65282.
KOiK04078.
OMAiQVTEMES.
OrthoDBiEOG09360K0I.
PhylomeDBiO65282.

Miscellaneous databases

PROiO65282.

Gene expression databases

GenevisibleiO65282. AT.

Family and domain databases

CDDicd00320. cpn10. 2 hits.
Gene3Di2.30.33.40. 2 hits.
HAMAPiMF_00580. CH10. 2 hits.
InterProiIPR020818. Chaperonin_GroES.
IPR018369. Chaprnonin_Cpn10_CS.
IPR017416. Cpn20.
IPR011032. GroES-like.
[Graphical view]
PANTHERiPTHR10772. PTHR10772. 1 hit.
PfamiPF00166. Cpn10. 2 hits.
[Graphical view]
PIRSFiPIRSF038157. Chaperonin_21_chloroplast. 1 hit.
PRINTSiPR00297. CHAPERONIN10.
SMARTiSM00883. Cpn10. 2 hits.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
PROSITEiPS00681. CHAPERONINS_CPN10. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCH20_ARATH
AccessioniPrimary (citable) accession number: O65282
Secondary accession number(s): Q9TNN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: December 8, 2000
Last modified: November 30, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.