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Protein

20 kDa chaperonin, chloroplastic

Gene

CPN20

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Seems to function only as a co-chaperone, along with CPN60, and in certain cases is essential for the discharge of biologically active proteins from CPN60 (PubMed:17178727, PubMed:23057508). Required to activate the iron superoxide dismutases (FeSOD) (PubMed:23057508).2 Publications
Involved in abscisic acid (ABA) signaling, independently of its co-chaperone role. Acts as negative regulator of the CHLH-WRKY40 coupled ABA signaling pathway, downstream of CHLH and upstream of WRKY40.1 Publication

GO - Molecular functioni

  • ATP binding Source: InterPro
  • chaperone binding Source: GO_Central
  • copper ion binding Source: TAIR
  • unfolded protein binding Source: GO_Central

GO - Biological processi

  • chaperone cofactor-dependent protein refolding Source: GO_Central
  • negative regulation of abscisic acid-activated signaling pathway Source: UniProtKB
  • positive regulation of superoxide dismutase activity Source: TAIR
  • protein heterotetramerization Source: UniProtKB
  • response to cadmium ion Source: TAIR
  • response to cold Source: TAIR
  • response to unfolded protein Source: GO_Central

Keywordsi

Molecular functionChaperone
Biological processStress response

Names & Taxonomyi

Protein namesi
Recommended name:
20 kDa chaperonin, chloroplastic
Alternative name(s):
Chaperonin 10
Short name:
Ch-CPN10
Short name:
Cpn10
Chaperonin 20
Protein Cpn21
Gene namesi
Name:CPN20
Synonyms:CHCPN10, CPN21
Ordered Locus Names:At5g20720
ORF Names:T1M15.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G20720
TAIRilocus:2180454 AT5G20720

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi83G → A: Reduces co-chaperone activity more than 20-fold. 1 Publication1
Mutagenesisi86L → A: Reduces co-chaperone activity more than 20-fold. 1 Publication1
Mutagenesisi181G → A: Reduces co-chaperone activity more than 3-fold. 1 Publication1
Mutagenesisi184L → A: Reduces co-chaperone activity more than 20-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 50ChloroplastSequence analysisAdd BLAST50
ChainiPRO_000000504551 – 25320 kDa chaperonin, chloroplasticAdd BLAST203

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei212PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO65282
PRIDEiO65282

2D gel databases

SWISS-2DPAGEiO65282

PTM databases

iPTMnetiO65282

Expressioni

Tissue specificityi

Ubiquitous. Most abundant in leaves and inflorescence. Low levels found in roots.1 Publication

Inductioni

Induced by heat treatment.1 Publication

Gene expression databases

ExpressionAtlasiO65282 baseline and differential
GenevisibleiO65282 AT

Interactioni

Subunit structurei

Homotetramer. Forms stable complexes with CPN60 in the presence of ATP (PubMed:10205903). Interacts with FSD1 (PubMed:23057508). Interacts with CLPT1 and CLPT2 (PubMed:25921872). Interacts with CHLH (PubMed:23783410).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
FSD1P212762EBI-4435709,EBI-4466816

GO - Molecular functioni

Protein-protein interaction databases

BioGridi17470, 5 interactors
IntActiO65282, 5 interactors
STRINGi3702.AT5G20720.1

Structurei

3D structure databases

ProteinModelPortaliO65282
SMRiO65282
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni60 – 153Cpn-10 domain 1CuratedAdd BLAST94
Regioni159 – 252Cpn-10 domain 2CuratedAdd BLAST94

Sequence similaritiesi

Belongs to the GroES chaperonin family.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG1641 Eukaryota
COG0234 LUCA
HOGENOMiHOG000133897
InParanoidiO65282
KOiK04078
OMAiTHSINSY
OrthoDBiEOG09360K0I
PhylomeDBiO65282

Family and domain databases

CDDicd00320 cpn10, 2 hits
Gene3Di2.30.33.40, 2 hits
HAMAPiMF_00580 CH10, 2 hits
InterProiView protein in InterPro
IPR020818 Chaperonin_GroES
IPR037124 Chaperonin_GroES_sf
IPR018369 Chaprnonin_Cpn10_CS
IPR017416 Cpn20
IPR011032 GroES-like_sf
PANTHERiPTHR10772 PTHR10772, 1 hit
PfamiView protein in Pfam
PF00166 Cpn10, 2 hits
PIRSFiPIRSF038157 Chaperonin_21_chloroplast, 1 hit
PRINTSiPR00297 CHAPERONIN10
SMARTiView protein in SMART
SM00883 Cpn10, 2 hits
SUPFAMiSSF50129 SSF50129, 2 hits
PROSITEiView protein in PROSITE
PS00681 CHAPERONINS_CPN10, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O65282-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATQLTASP VTMSARSLAS LDGLRASSVK FSSLKPGTLR QSQFRRLVVK
60 70 80 90 100
AASVVAPKYT SIKPLGDRVL VKIKEAEEKT LGGILLPSTA QSKPQGGEVV
110 120 130 140 150
AVGEGRTIGK NKIDITVPTG AQIIYSKYAG TEVEFNDVKH LILKEDDIVG
160 170 180 190 200
ILETEDIKDL KPLNDRVFIK VAEAEEKTAG GLLLTETTKE KPSIGTVIAV
210 220 230 240 250
GPGSLDEEGK ITPLPVSTGS TVLYSKYAGN DFKGKDGSNY IALRASDVMA

ILS
Length:253
Mass (Da):26,802
Last modified:December 8, 2000 - v2
Checksum:i43A8CD78E7C95BCC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31F → V in AAC14026 (Ref. 3) Curated1
Sequence conflicti86 – 87LP → TFH in AAC14026 (Ref. 3) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010818 mRNA Translation: CAA09368.1
AF268068 mRNA Translation: AAG13931.1
AF059037 mRNA Translation: AAC14026.1
AB007130 mRNA Translation: BAB61619.1
AF296832 Genomic DNA No translation available.
CP002688 Genomic DNA Translation: AED92881.1
CP002688 Genomic DNA Translation: AED92882.1
CP002688 Genomic DNA Translation: AED92883.1
CP002688 Genomic DNA Translation: ANM70274.1
AY062971 mRNA Translation: AAL33817.1
AY034979 mRNA Translation: AAK59484.1
AF428366 mRNA Translation: AAL16296.1
AF428339 mRNA Translation: AAL16269.1
PIRiT52122
T52613
RefSeqiNP_001318614.1, NM_001343684.1
NP_001331898.1, NM_001343685.1
NP_197572.1, NM_122079.4
NP_851045.1, NM_180714.4
UniGeneiAt.49045
At.59645

Genome annotation databases

EnsemblPlantsiAT5G20720.1; AT5G20720.1; AT5G20720
AT5G20720.2; AT5G20720.2; AT5G20720
AT5G20720.3; AT5G20720.3; AT5G20720
AT5G20720.4; AT5G20720.4; AT5G20720
GeneIDi832195
GrameneiAT5G20720.1; AT5G20720.1; AT5G20720
AT5G20720.2; AT5G20720.2; AT5G20720
AT5G20720.3; AT5G20720.3; AT5G20720
AT5G20720.4; AT5G20720.4; AT5G20720
KEGGiath:AT5G20720

Similar proteinsi

Entry informationi

Entry nameiCH20_ARATH
AccessioniPrimary (citable) accession number: O65282
Secondary accession number(s): Q9TNN2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: December 8, 2000
Last modified: April 25, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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