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Protein

Bifunctional protein FolD 4, chloroplastic

Gene

FOLD4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.By similarity

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Oxidoreductase
Biological processOne-carbon metabolism, Photorespiration
LigandNADP

Enzyme and pathway databases

BioCyciARA:AT4G00620-MONOMER.
ReactomeiR-ATH-196757. Metabolism of folate and pterines.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolD 4, chloroplastic
Alternative name(s):
Protein EMBRYO DEFECTIVE 3127
Tetrahydrofolate dehydrogenase/cyclohydrolase 4
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenase (EC:1.5.1.5)
Methenyltetrahydrofolate cyclohydrolase (EC:3.5.4.9)
Gene namesi
Name:FOLD4
Synonyms:DHC4, EMB3127
Ordered Locus Names:At4g00620
ORF Names:F6N23.26
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G00620.
TAIRilocus:2127113. AT4G00620.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 51ChloroplastCombined sourcesAdd BLAST51
ChainiPRO_000042434752 – 360Bifunctional protein FolD 4, chloroplasticAdd BLAST309

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO65271.
PRIDEiO65271.

PTM databases

iPTMnetiO65271.

Expressioni

Gene expression databases

ExpressionAtlasiO65271. baseline and differential.
GenevisibleiO65271. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT4G00620.1.

Structurei

3D structure databases

ProteinModelPortaliO65271.
SMRiO65271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0089. Eukaryota.
COG0190. LUCA.
HOGENOMiHOG000218242.
InParanoidiO65271.
OMAiGIPKLIT.
OrthoDBiEOG09360FBO.
PhylomeDBiO65271.

Family and domain databases

HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiView protein in InterPro
IPR036291. NAD(P)-bd_dom_sf.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
PANTHERiPTHR10025. PTHR10025. 1 hit.
PfamiView protein in Pfam
PF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiView protein in PROSITE
PS00767. THF_DHG_CYH_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O65271-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASMMFTDCS STTTSRLIHL NRSSGTFLLR QCVGQLRLQT TASGRGCCIR
60 70 80 90 100
SSSSPISSIS ADTKSEGGAI VIDGKAVAKK IRDEITIEVS RMKESIGVIP
110 120 130 140 150
GLAVILVGDR KDSATYVRNK KKACDSVGIK SFEVRLAEDS SEEEVLKSVS
160 170 180 190 200
GFNDDPSVHG ILVQLPLPSH MDEQNILNAV SIEKDVDGFH PLNIGRLAMR
210 220 230 240 250
GREPLFVPCT PKGCIELLHR YNIEIKGKRA VVIGRSNIVG MPAALLLQRE
260 270 280 290 300
DATVSIIHSR TKNPEEITRE ADIIISAVGQ PNMVRGSWIK PGAVLIDVGI
310 320 330 340 350
NPVEDPSAAR GYRLVGDICY EEASKVASAI TPVPGGVGPM TIAMLLSNTL
360
TSAKRIHNFQ
Length:360
Mass (Da):38,742
Last modified:August 1, 1998 - v1
Checksum:i15D254E1DDD2F6B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058919 Genomic DNA. Translation: AAC13627.1.
AL161472 Genomic DNA. Translation: CAB80871.1.
CP002687 Genomic DNA. Translation: AEE81910.1.
AY059944 mRNA. Translation: AAL24426.1.
BT000066 mRNA. Translation: AAN15385.1.
PIRiT01226.
RefSeqiNP_191971.1. NM_116287.4.
UniGeneiAt.3767.

Genome annotation databases

EnsemblPlantsiAT4G00620.1; AT4G00620.1; AT4G00620.
GeneIDi825824.
GrameneiAT4G00620.1; AT4G00620.1; AT4G00620.
KEGGiath:AT4G00620.

Similar proteinsi

Entry informationi

Entry nameiFOLD4_ARATH
AccessioniPrimary (citable) accession number: O65271
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: August 1, 1998
Last modified: October 25, 2017
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families