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Protein

Bifunctional protein FolD 3, chloroplastic

Gene

FOLD3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.By similarity

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Oxidoreductase
Biological processOne-carbon metabolism, Photorespiration
LigandNADP

Enzyme and pathway databases

BioCyciARA:AT4G00600-MONOMER
ReactomeiR-ATH-196757 Metabolism of folate and pterines
UniPathwayiUPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolD 3, chloroplastic
Alternative name(s):
Tetrahydrofolate dehydrogenase/cyclohydrolase 3
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenase (EC:1.5.1.5)
Methenyltetrahydrofolate cyclohydrolase (EC:3.5.4.9)
Gene namesi
Name:FOLD3
Synonyms:DHC3
Ordered Locus Names:At4g00600
ORF Names:F6N23.28
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G00600
TAIRilocus:2127083 AT4G00600

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 48ChloroplastSequence analysisAdd BLAST48
ChainiPRO_000042434649 – 310Bifunctional protein FolD 3, chloroplasticAdd BLAST262

Proteomic databases

PaxDbiO65269

Expressioni

Gene expression databases

ExpressionAtlasiO65269 baseline and differential
GenevisibleiO65269 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT4G00600.1

Structurei

3D structure databases

ProteinModelPortaliO65269
SMRiO65269
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi49 – 54Poly-Ser6

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0089 Eukaryota
COG0190 LUCA
HOGENOMiHOG000218242
InParanoidiO65269
OMAiSKTRACE
OrthoDBiEOG09360FBO
PhylomeDBiO65269

Family and domain databases

HAMAPiMF_01576 THF_DHG_CYH, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR000672 THF_DH/CycHdrlase
IPR020630 THF_DH/CycHdrlase_cat_dom
IPR020631 THF_DH/CycHdrlase_NAD-bd_dom
PANTHERiPTHR10025 PTHR10025, 2 hits
PfamiView protein in Pfam
PF00763 THF_DHG_CYH, 1 hit
PF02882 THF_DHG_CYH_C, 1 hit
PRINTSiPR00085 THFDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O65269-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTDCSSSTT SRLIHLYNRN GVFLPRPSVS QFSLRTTAST WRCTLSIRSS
60 70 80 90 100
SSPSAIVIDG KAEAKKIRDD IKIEVSRMKE SIGVVPAEDS SEEEVLKYVS
110 120 130 140 150
GFNDDPSVHG VLVQLPLPSH MDEQNILNAV SIEKDVDGFH PLNIGRLAMR
160 170 180 190 200
GREPLFVPCT PKGCIELLHR YNIEFKGKRA VVIGRSNIVG MPAALLLQKE
210 220 230 240 250
DATVSIIHSR TMNPEELTRQ ADILISAVGK PNMVRGSWIK PGAVLIDVGI
260 270 280 290 300
KPVEDPSAAG GERLVGDICY VEASKIASAI TPVPGDVGPM TIAMLLSNTL
310
TSAKRIHNFQ
Length:310
Mass (Da):33,696
Last modified:August 1, 1998 - v1
Checksum:i9A71117B64896FBC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058919 Genomic DNA Translation: AAC13632.1
AL161472 Genomic DNA Translation: CAB80869.1
CP002687 Genomic DNA Translation: AEE81908.1
PIRiT01224
RefSeqiNP_191969.1, NM_116285.1
UniGeneiAt.54064

Genome annotation databases

EnsemblPlantsiAT4G00600.1; AT4G00600.1; AT4G00600
GeneIDi825813
GrameneiAT4G00600.1; AT4G00600.1; AT4G00600
KEGGiath:AT4G00600

Similar proteinsi

Entry informationi

Entry nameiFOLD3_ARATH
AccessioniPrimary (citable) accession number: O65269
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health