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Protein

Bifunctional protein FolD 3, chloroplastic

Gene

FOLD3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.By similarity

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Oxidoreductase

Keywords - Biological processi

One-carbon metabolism, Photorespiration

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciARA:AT4G00600-MONOMER.
ReactomeiR-ATH-196757. Metabolism of folate and pterines.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolD 3, chloroplastic
Alternative name(s):
Tetrahydrofolate dehydrogenase/cyclohydrolase 3
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenase (EC:1.5.1.5)
Methenyltetrahydrofolate cyclohydrolase (EC:3.5.4.9)
Gene namesi
Name:FOLD3
Synonyms:DHC3
Ordered Locus Names:At4g00600
ORF Names:F6N23.28
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G00600.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 48ChloroplastSequence analysisAdd BLAST48
ChainiPRO_000042434649 – 310Bifunctional protein FolD 3, chloroplasticAdd BLAST262

Proteomic databases

PaxDbiO65269.

Expressioni

Gene expression databases

GenevisibleiO65269. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT4G00600.1.

Structurei

3D structure databases

ProteinModelPortaliO65269.
SMRiO65269.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi49 – 54Poly-Ser6

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0089. Eukaryota.
COG0190. LUCA.
HOGENOMiHOG000218242.
InParanoidiO65269.
OMAiRTTASTW.
OrthoDBiEOG09360FBO.
PhylomeDBiO65269.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O65269-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTDCSSSTT SRLIHLYNRN GVFLPRPSVS QFSLRTTAST WRCTLSIRSS
60 70 80 90 100
SSPSAIVIDG KAEAKKIRDD IKIEVSRMKE SIGVVPAEDS SEEEVLKYVS
110 120 130 140 150
GFNDDPSVHG VLVQLPLPSH MDEQNILNAV SIEKDVDGFH PLNIGRLAMR
160 170 180 190 200
GREPLFVPCT PKGCIELLHR YNIEFKGKRA VVIGRSNIVG MPAALLLQKE
210 220 230 240 250
DATVSIIHSR TMNPEELTRQ ADILISAVGK PNMVRGSWIK PGAVLIDVGI
260 270 280 290 300
KPVEDPSAAG GERLVGDICY VEASKIASAI TPVPGDVGPM TIAMLLSNTL
310
TSAKRIHNFQ
Length:310
Mass (Da):33,696
Last modified:August 1, 1998 - v1
Checksum:i9A71117B64896FBC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058919 Genomic DNA. Translation: AAC13632.1.
AL161472 Genomic DNA. Translation: CAB80869.1.
CP002687 Genomic DNA. Translation: AEE81908.1.
PIRiT01224.
RefSeqiNP_191969.1. NM_116285.1.
UniGeneiAt.54064.

Genome annotation databases

EnsemblPlantsiAT4G00600.1; AT4G00600.1; AT4G00600.
GeneIDi825813.
GrameneiAT4G00600.1; AT4G00600.1; AT4G00600.
KEGGiath:AT4G00600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058919 Genomic DNA. Translation: AAC13632.1.
AL161472 Genomic DNA. Translation: CAB80869.1.
CP002687 Genomic DNA. Translation: AEE81908.1.
PIRiT01224.
RefSeqiNP_191969.1. NM_116285.1.
UniGeneiAt.54064.

3D structure databases

ProteinModelPortaliO65269.
SMRiO65269.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G00600.1.

Proteomic databases

PaxDbiO65269.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G00600.1; AT4G00600.1; AT4G00600.
GeneIDi825813.
GrameneiAT4G00600.1; AT4G00600.1; AT4G00600.
KEGGiath:AT4G00600.

Organism-specific databases

TAIRiAT4G00600.

Phylogenomic databases

eggNOGiKOG0089. Eukaryota.
COG0190. LUCA.
HOGENOMiHOG000218242.
InParanoidiO65269.
OMAiRTTASTW.
OrthoDBiEOG09360FBO.
PhylomeDBiO65269.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciARA:AT4G00600-MONOMER.
ReactomeiR-ATH-196757. Metabolism of folate and pterines.

Miscellaneous databases

PROiO65269.

Gene expression databases

GenevisibleiO65269. AT.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFOLD3_ARATH
AccessioniPrimary (citable) accession number: O65269
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.