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Protein

Beta-amylase 2, chloroplastic

Gene

BAM2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Low beta-amylase activity. Interacts poorly with starch or other alpha-1,4-glucan.2 Publications

Catalytic activityi

Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.

Enzyme regulationi

Redox regulation; active in reducing conditions, inactive in oxidizing conditions.By similarity

pH dependencei

Optimum pH is 6.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei136SubstrateBy similarity1
Binding sitei176SubstrateBy similarity1
Binding sitei184SubstrateBy similarity1
Active sitei269Proton donorBy similarity1
Binding sitei377SubstrateBy similarity1
Binding sitei382SubstrateBy similarity1
Binding sitei424SubstrateBy similarity1
Active sitei465Proton acceptorBy similarity1
Binding sitei501SubstrateBy similarity1

GO - Molecular functioni

  • amylopectin maltohydrolase activity Source: UniProtKB-EC
  • beta-amylase activity Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH14 Glycoside Hydrolase Family 14

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-amylase 2, chloroplastic (EC:3.2.1.2)
Alternative name(s):
1,4-alpha-D-glucan maltohydrolase
Beta-amylase 9
Gene namesi
Name:BAM2
Synonyms:BMY9
Ordered Locus Names:At4g00490
ORF Names:F6N23.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G00490
TAIRilocus:2127033 AT4G00490

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Normal growth rate and starch breakdown in leaves during the night.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 55ChloroplastSequence analysisAdd BLAST55
ChainiPRO_000039341756 – 542Beta-amylase 2, chloroplasticAdd BLAST487

Proteomic databases

PaxDbiO65258
PRIDEiO65258

PTM databases

iPTMnetiO65258

Expressioni

Inductioni

Slightly by cold stress.1 Publication

Gene expression databases

ExpressionAtlasiO65258 baseline and differential
GenevisibleiO65258 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G00490.1

Structurei

3D structure databases

ProteinModelPortaliO65258
SMRiO65258
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni466 – 467Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 14 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IECX Eukaryota
ENOG410XNXS LUCA
HOGENOMiHOG000238755
KOiK01177
OMAiDQHEVFP
OrthoDBiEOG093605C7
PhylomeDBiO65258

Family and domain databases

InterProiView protein in InterPro
IPR001554 Glyco_hydro_14
IPR018238 Glyco_hydro_14_CS
IPR001371 Glyco_hydro_14B_pln
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR31352 PTHR31352, 1 hit
PfamiView protein in Pfam
PF01373 Glyco_hydro_14, 1 hit
PRINTSiPR00750 BETAAMYLASE
PR00842 GLHYDLASE14B
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00506 BETA_AMYLASE_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O65258-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIRLNHSVI PVSVKLGAPT RVSARSSLPF SVGDWRGVST FSGARPLVLA
60 70 80 90 100
KVKLRAESTE EDRVPIDDDD DSTDQLVDEE IVHFEERDFA GTACVPVYVM
110 120 130 140 150
LPLGVIDMNS EVVEPEELLD QLRTLKSVNV DGVMVDCWWG IVESHTPQVY
160 170 180 190 200
NWSGYKKLFQ MIRELGLKIQ VVMSFHECGG NVGDDVHIQI PEWVREIGQS
210 220 230 240 250
NPDIYFTDSA GRRNTECLTW GIDKQRVLRG RTALEVYFDY MRSFRVEFDE
260 270 280 290 300
FFEEKIIPEI EVGLGPCGEL RYPSYPAQFG WKYPGIGEFQ CYDKYLMNSL
310 320 330 340 350
KEAAEVRGHS FWGRGPDNTE TYNSTPHGTG FFRDGGDYDS YYGRFFLNWY
360 370 380 390 400
SRVLIDHGDR VLAMANLAFE GTCIAAKLSG IHWWYKTASH AAELTAGFYN
410 420 430 440 450
SSNRDGYGPI AAMFKKHDAA LNFTCVELRT LDQHEDFPEA LADPEGLVWQ
460 470 480 490 500
VLNAAWDASI PVASENALPC YDREGYNKIL ENAKPLTDPD GRHLSCFTYL
510 520 530 540
RLNPTLMESQ NFKEFERFLK RMHGEAVPDL GLAPGTQETN PE
Length:542
Mass (Da):61,398
Last modified:April 20, 2010 - v2
Checksum:i892C9202CD34E1A9
GO

Sequence cautioni

The sequence AAC13634 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80858 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058919 Genomic DNA Translation: AAC13634.1 Sequence problems.
AL161472 Genomic DNA Translation: CAB80858.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE81889.1
PIRiT01213
RefSeqiNP_191958.3, NM_116273.5
UniGeneiAt.27432
At.68473

Genome annotation databases

EnsemblPlantsiAT4G00490.1; AT4G00490.1; AT4G00490
GeneIDi827959
GrameneiAT4G00490.1; AT4G00490.1; AT4G00490
KEGGiath:AT4G00490

Similar proteinsi

Entry informationi

Entry nameiBAM2_ARATH
AccessioniPrimary (citable) accession number: O65258
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: April 20, 2010
Last modified: April 25, 2018
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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