Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-amylase 2, chloroplastic

Gene

BAM2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Low beta-amylase activity. Interacts poorly with starch or other alpha-1,4-glucan.2 Publications

Catalytic activityi

Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.

Enzyme regulationi

Redox regulation; active in reducing conditions, inactive in oxidizing conditions.By similarity

pH dependencei

Optimum pH is 6.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei136 – 1361SubstrateBy similarity
Binding sitei176 – 1761SubstrateBy similarity
Binding sitei184 – 1841SubstrateBy similarity
Active sitei269 – 2691Proton donorBy similarity
Binding sitei377 – 3771SubstrateBy similarity
Binding sitei382 – 3821SubstrateBy similarity
Binding sitei424 – 4241SubstrateBy similarity
Active sitei465 – 4651Proton acceptorBy similarity
Binding sitei501 – 5011SubstrateBy similarity

GO - Molecular functioni

  • beta-amylase activity Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH14. Glycoside Hydrolase Family 14.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-amylase 2, chloroplastic (EC:3.2.1.2)
Alternative name(s):
1,4-alpha-D-glucan maltohydrolase
Beta-amylase 9
Gene namesi
Name:BAM2
Synonyms:BMY9
Ordered Locus Names:At4g00490
ORF Names:F6N23.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G00490.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Normal growth rate and starch breakdown in leaves during the night.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5555ChloroplastSequence analysisAdd
BLAST
Chaini56 – 542487Beta-amylase 2, chloroplasticPRO_0000393417Add
BLAST

Proteomic databases

PaxDbiO65258.
PRIDEiO65258.

PTM databases

iPTMnetiO65258.

Expressioni

Inductioni

Slightly by cold stress.1 Publication

Gene expression databases

GenevisibleiO65258. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G00490.1.

Structurei

3D structure databases

ProteinModelPortaliO65258.
SMRiO65258. Positions 95-534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni466 – 4672Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 14 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IECX. Eukaryota.
ENOG410XNXS. LUCA.
HOGENOMiHOG000238755.
KOiK01177.
OMAiDQHEVFP.
OrthoDBiEOG093605C7.
PhylomeDBiO65258.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001554. Glyco_hydro_14.
IPR018238. Glyco_hydro_14_CS.
IPR001371. Glyco_hydro_14B_pln.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01373. Glyco_hydro_14. 1 hit.
[Graphical view]
PRINTSiPR00750. BETAAMYLASE.
PR00842. GLHYDLASE14B.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00506. BETA_AMYLASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O65258-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIRLNHSVI PVSVKLGAPT RVSARSSLPF SVGDWRGVST FSGARPLVLA
60 70 80 90 100
KVKLRAESTE EDRVPIDDDD DSTDQLVDEE IVHFEERDFA GTACVPVYVM
110 120 130 140 150
LPLGVIDMNS EVVEPEELLD QLRTLKSVNV DGVMVDCWWG IVESHTPQVY
160 170 180 190 200
NWSGYKKLFQ MIRELGLKIQ VVMSFHECGG NVGDDVHIQI PEWVREIGQS
210 220 230 240 250
NPDIYFTDSA GRRNTECLTW GIDKQRVLRG RTALEVYFDY MRSFRVEFDE
260 270 280 290 300
FFEEKIIPEI EVGLGPCGEL RYPSYPAQFG WKYPGIGEFQ CYDKYLMNSL
310 320 330 340 350
KEAAEVRGHS FWGRGPDNTE TYNSTPHGTG FFRDGGDYDS YYGRFFLNWY
360 370 380 390 400
SRVLIDHGDR VLAMANLAFE GTCIAAKLSG IHWWYKTASH AAELTAGFYN
410 420 430 440 450
SSNRDGYGPI AAMFKKHDAA LNFTCVELRT LDQHEDFPEA LADPEGLVWQ
460 470 480 490 500
VLNAAWDASI PVASENALPC YDREGYNKIL ENAKPLTDPD GRHLSCFTYL
510 520 530 540
RLNPTLMESQ NFKEFERFLK RMHGEAVPDL GLAPGTQETN PE
Length:542
Mass (Da):61,398
Last modified:April 20, 2010 - v2
Checksum:i892C9202CD34E1A9
GO

Sequence cautioni

The sequence AAC13634 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB80858 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058919 Genomic DNA. Translation: AAC13634.1. Sequence problems.
AL161472 Genomic DNA. Translation: CAB80858.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE81889.1.
PIRiT01213.
RefSeqiNP_191958.3. NM_116273.4.
UniGeneiAt.27432.
At.68473.

Genome annotation databases

EnsemblPlantsiAT4G00490.1; AT4G00490.1; AT4G00490.
GeneIDi827959.
GrameneiAT4G00490.1; AT4G00490.1; AT4G00490.
KEGGiath:AT4G00490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058919 Genomic DNA. Translation: AAC13634.1. Sequence problems.
AL161472 Genomic DNA. Translation: CAB80858.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE81889.1.
PIRiT01213.
RefSeqiNP_191958.3. NM_116273.4.
UniGeneiAt.27432.
At.68473.

3D structure databases

ProteinModelPortaliO65258.
SMRiO65258. Positions 95-534.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G00490.1.

Protein family/group databases

CAZyiGH14. Glycoside Hydrolase Family 14.

PTM databases

iPTMnetiO65258.

Proteomic databases

PaxDbiO65258.
PRIDEiO65258.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G00490.1; AT4G00490.1; AT4G00490.
GeneIDi827959.
GrameneiAT4G00490.1; AT4G00490.1; AT4G00490.
KEGGiath:AT4G00490.

Organism-specific databases

TAIRiAT4G00490.

Phylogenomic databases

eggNOGiENOG410IECX. Eukaryota.
ENOG410XNXS. LUCA.
HOGENOMiHOG000238755.
KOiK01177.
OMAiDQHEVFP.
OrthoDBiEOG093605C7.
PhylomeDBiO65258.

Miscellaneous databases

PROiO65258.

Gene expression databases

GenevisibleiO65258. AT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001554. Glyco_hydro_14.
IPR018238. Glyco_hydro_14_CS.
IPR001371. Glyco_hydro_14B_pln.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01373. Glyco_hydro_14. 1 hit.
[Graphical view]
PRINTSiPR00750. BETAAMYLASE.
PR00842. GLHYDLASE14B.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00506. BETA_AMYLASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBAM2_ARATH
AccessioniPrimary (citable) accession number: O65258
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: April 20, 2010
Last modified: September 7, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.