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Protein

Acyl-coenzyme A oxidase 2, peroxisomal

Gene

ACX2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the desaturation of long-chain acyl-CoAs to 2-trans-enoyl-CoAs. Active on substrates longer than C14 and mostly with C18-CoA. Activity on long-chain mono-unsaturated substrates is double than with the corresponding saturated substrates.

Catalytic activityi

Acyl-CoA + O2 = trans-2,3-dehydroacyl-CoA + H2O2.

Cofactori

Kineticsi

  1. KM=4.4 µM for C18:1-CoA

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi450 – 455FAD6

    GO - Molecular functioni

    GO - Biological processi

    • fatty acid beta-oxidation Source: TAIR
    • fatty acid beta-oxidation using acyl-CoA dehydrogenase Source: GO_Central
    • lipid homeostasis Source: GO_Central
    • long-chain fatty acid metabolic process Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciARA:AT5G65110-MONOMER.
    MetaCyc:AT5G65110-MONOMER.
    BRENDAi1.3.3.6. 399.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyl-coenzyme A oxidase 2, peroxisomal (EC:1.3.3.6)
    Short name:
    AOX 2
    Alternative name(s):
    Long-chain acyl-CoA oxidase
    Short name:
    AtCX2
    Gene namesi
    Name:ACX2
    Ordered Locus Names:At5g65110
    ORF Names:MQN23.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    TAIRiAT5G65110.

    Subcellular locationi

    GO - Cellular componenti

    • peroxisome Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 49PeroxisomeBy similarityAdd BLAST49
    ChainiPRO_000000055550 – 692Acyl-coenzyme A oxidase 2, peroxisomalAdd BLAST643

    Proteomic databases

    PaxDbiO65201.
    PRIDEiO65201.

    Expressioni

    Tissue specificityi

    Expressed mainly in flowers and young seedlings. Lower expression in roots, leaves and bracts.1 Publication

    Developmental stagei

    Induced by seed imbibition with a peak at day 2 and then declines steadily until day 8.1 Publication

    Inductioni

    Induced by dehydration and abscisic acid (ABA).1 Publication

    Gene expression databases

    ExpressionAtlasiO65201. baseline and differential.
    GenevisibleiO65201. AT.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi3702.AT5G65110.1.

    Structurei

    3D structure databases

    ProteinModelPortaliO65201.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the acyl-CoA oxidase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiKOG0135. Eukaryota.
    COG1960. LUCA.
    HOGENOMiHOG000245077.
    InParanoidiO65201.
    KOiK00232.
    OMAiMKKTHDE.
    OrthoDBiEOG0936039J.
    PhylomeDBiO65201.

    Family and domain databases

    InterProiIPR006091. Acyl-CoA_Oxase/DH_cen-dom.
    IPR012258. Acyl-CoA_oxidase.
    IPR002655. Acyl-CoA_oxidase_C.
    IPR009075. AcylCo_DH/oxidase_C.
    IPR009100. AcylCoA_DH/oxidase_NM_dom.
    [Graphical view]
    PfamiPF01756. ACOX. 1 hit.
    PF00441. Acyl-CoA_dh_1. 1 hit.
    PF02770. Acyl-CoA_dh_M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000168. Acyl-CoA_oxidase. 1 hit.
    SUPFAMiSSF47203. SSF47203. 2 hits.
    SSF56645. SSF56645. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced. According to EST sequences.
    Isoform 1 (identifier: O65201-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MESRREKNPM TEEESDGLIA ARRIQRLSLH LSPSLTPSPS LPLVQTETCS
    60 70 80 90 100
    ARSKKLDVNG EALSLYMRGK HIDIQEKIFD FFNSRPDLQT PIEISKDDHR
    110 120 130 140 150
    ELCMNQLIGL VREAGVRPFR YVADDPEKYF AIMEAVGSVD MSLGIKMGVQ
    160 170 180 190 200
    YSLWGGSVIN LGTKKHRDKY FDGIDNLDYT GCFAMTELHH GSNVQGLQTT
    210 220 230 240 250
    ATFDPLKDEF VIDTPNDGAI KWWIGNAAVH GKFATVFARL ILPTHDSKGV
    260 270 280 290 300
    SDMGVHAFIV PIRDMKTHQT LPGVEIQDCG HKVGLNGVDN GALRFRSVRI
    310 320 330 340 350
    PRDNLLNRFG DVSRDGTYTS SLPTINKRFG ATLGELVGGR VGLAYASVGV
    360 370 380 390 400
    LKISATIAIR YSLLRQQFGP PKQPEVSILD YQSQQHKLMP MLASTYAYHF
    410 420 430 440 450
    ATVYLVEKYS EMKKTHDEQL VADVHALSAG LKSYVTSYTA KALSVCREAC
    460 470 480 490 500
    GGHGYAAVNR FGSLRNDHDI FQTFEGDNTV LLQQVAADLL KRYKEKFQGG
    510 520 530 540 550
    TLTVTWSYLR ESMNTYLSQP NPVTARWEGE DHLRDPKFQL DAFRYRTSRL
    560 570 580 590 600
    LQNVAARLQK HSKTLGGFGA WNRCLNHLLT LAESHIETVI LAKFIEAVKN
    610 620 630 640 650
    CPDPSAKAAL KLACDLYALD RIWKDIGTYR NVDYVAPNKA KAIHKLTEYL
    660 670 680 690
    SFQVRNVAKE LVDAFELPDH VTRAPIAMQS DAYSQYTQVV GF
    Length:692
    Mass (Da):77,480
    Last modified:March 29, 2005 - v2
    Checksum:i43B29F0E8D3176D7
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti37P → L in AAC13497 (PubMed:10571860).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF057043 mRNA. Translation: AAC13497.1.
    AB013395 Genomic DNA. Translation: BAB11647.1.
    CP002688 Genomic DNA. Translation: AED98003.1.
    PIRiT52120.
    RefSeqiNP_201316.1. NM_125910.6. [O65201-1]
    UniGeneiAt.49226.

    Genome annotation databases

    EnsemblPlantsiAT5G65110.1; AT5G65110.1; AT5G65110. [O65201-1]
    GeneIDi836635.
    GrameneiAT5G65110.1; AT5G65110.1; AT5G65110.
    KEGGiath:AT5G65110.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF057043 mRNA. Translation: AAC13497.1.
    AB013395 Genomic DNA. Translation: BAB11647.1.
    CP002688 Genomic DNA. Translation: AED98003.1.
    PIRiT52120.
    RefSeqiNP_201316.1. NM_125910.6. [O65201-1]
    UniGeneiAt.49226.

    3D structure databases

    ProteinModelPortaliO65201.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT5G65110.1.

    Proteomic databases

    PaxDbiO65201.
    PRIDEiO65201.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT5G65110.1; AT5G65110.1; AT5G65110. [O65201-1]
    GeneIDi836635.
    GrameneiAT5G65110.1; AT5G65110.1; AT5G65110.
    KEGGiath:AT5G65110.

    Organism-specific databases

    TAIRiAT5G65110.

    Phylogenomic databases

    eggNOGiKOG0135. Eukaryota.
    COG1960. LUCA.
    HOGENOMiHOG000245077.
    InParanoidiO65201.
    KOiK00232.
    OMAiMKKTHDE.
    OrthoDBiEOG0936039J.
    PhylomeDBiO65201.

    Enzyme and pathway databases

    BioCyciARA:AT5G65110-MONOMER.
    MetaCyc:AT5G65110-MONOMER.
    BRENDAi1.3.3.6. 399.

    Miscellaneous databases

    PROiO65201.

    Gene expression databases

    ExpressionAtlasiO65201. baseline and differential.
    GenevisibleiO65201. AT.

    Family and domain databases

    InterProiIPR006091. Acyl-CoA_Oxase/DH_cen-dom.
    IPR012258. Acyl-CoA_oxidase.
    IPR002655. Acyl-CoA_oxidase_C.
    IPR009075. AcylCo_DH/oxidase_C.
    IPR009100. AcylCoA_DH/oxidase_NM_dom.
    [Graphical view]
    PfamiPF01756. ACOX. 1 hit.
    PF00441. Acyl-CoA_dh_1. 1 hit.
    PF02770. Acyl-CoA_dh_M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000168. Acyl-CoA_oxidase. 1 hit.
    SUPFAMiSSF47203. SSF47203. 2 hits.
    SSF56645. SSF56645. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiACOX2_ARATH
    AccessioniPrimary (citable) accession number: O65201
    Secondary accession number(s): Q9FJQ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: March 29, 2005
    Last modified: November 30, 2016
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.