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Protein

NEDD8-activating enzyme E1 catalytic subunit

Gene

ECR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of the dimeric ECR1-AXR1 E1 enzyme. E1 activates NEDD8/RUB1 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-ECR1 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of RCE1 (By similarity).By similarity2 Publications

Miscellaneous

The formation of the adenylate intermediate is possible in absence of AXR1 and without the participation of Cys-215.

Pathwayi: protein neddylation

This protein is involved in the pathway protein neddylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein neddylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei215Glycyl thioester intermediate1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi56 – 80ATPBy similarityAdd BLAST25

GO - Molecular functioni

  • acid-amino acid ligase activity Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • NEDD8 activating enzyme activity Source: TAIR
  • protein heterodimerization activity Source: TAIR

GO - Biological processi

  • protein neddylation Source: TAIR

Keywordsi

Molecular functionLigase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-8951664 Neddylation
UniPathwayiUPA00885

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8-activating enzyme E1 catalytic subunit (EC:6.2.1.-)
Alternative name(s):
RUB-activating enzyme
Ubiquitin-activating enzyme E1-like protein
Gene namesi
Name:ECR1
Ordered Locus Names:At5g19180
ORF Names:T24G5_80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G19180
TAIRilocus:2182172 AT5G19180

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi215C → A: Loss of binding to RUB1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001949482 – 454NEDD8-activating enzyme E1 catalytic subunitAdd BLAST453

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO65041
PRIDEiO65041

PTM databases

iPTMnetiO65041

Expressioni

Tissue specificityi

Expressed in shoot, root and floral meristems, in vascular tissues of cotyledons and mature leaves, and in the stele of the root.1 Publication

Developmental stagei

Expressed during ovules and embryo development.1 Publication

Inductioni

No accumulation in response to auxin treatment.

Gene expression databases

ExpressionAtlasiO65041 baseline and differential
GenevisibleiO65041 AT

Interactioni

Subunit structurei

Heterodimer of UBA3/ECR1 and AXR1. Interacts with NEDD8 and RCE1.1 Publication

GO - Molecular functioni

  • protein heterodimerization activity Source: TAIR

Protein-protein interaction databases

STRINGi3702.AT5G19180.1

Structurei

3D structure databases

ProteinModelPortaliO65041
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2015 Eukaryota
COG0476 LUCA
HOGENOMiHOG000166793
InParanoidiO65041
KOiK10686
OMAiIAASCCN
OrthoDBiEOG09360EWD
PhylomeDBiO65041

Family and domain databases

Gene3Di1.10.10.520, 1 hit
InterProiView protein in InterPro
IPR014929 E2_binding
IPR000594 ThiF_NAD_FAD-bd
IPR023318 Ub_act_enz_dom_a_sf
IPR030468 Uba3
IPR035985 Ubiquitin-activating_enz
IPR033127 UBQ-activ_enz_E1_Cys_AS
PANTHERiPTHR10953:SF6 PTHR10953:SF6, 1 hit
PfamiView protein in Pfam
PF08825 E2_bind, 1 hit
PF00899 ThiF, 1 hit
SMARTiView protein in SMART
SM01181 E2_bind, 1 hit
SUPFAMiSSF69572 SSF69572, 1 hit
PROSITEiView protein in PROSITE
PS00865 UBIQUITIN_ACTIVAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O65041-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADLDVPPQV PQSKTRDLDK LLLRHGNLVD PGFVPGPGLR DDIRDYVRIL
60 70 80 90 100
VIGAGGLGCE LLKDLALSGF RNLEVIDMDR IEVTNLNRQF LFRIEDVGKP
110 120 130 140 150
KAEVAAKRVM ERVSGVEIVP HFSRIEDKEI EFYNDFNIIA LGLDSIEARK
160 170 180 190 200
YINGVACGFL EYNEDDTPKR ETIKPMVDGG TEGFKGHARV ILPGVTPCFE
210 220 230 240 250
CTIYLFPPQV KFPLCTLAET PRNAAHCIEY AHLIQWETVH RGKTFDPDEP
260 270 280 290 300
EHMKWVYDEA IRRAELFGIP GVTYSLTQGV VKNIIPAIAS TNAIISAACA
310 320 330 340 350
LETLKIVSAC SKTLVNYLTY NGGEGLYTEV TKFERDTECL VCGPGILIEL
360 370 380 390 400
DTSVTLSKFI EMLEDHPKLL LSKASVKQGE NTLYMQAPPV LEEFHRPKLS
410 420 430 440 450
KPLYDLMGRV QKDTIHVFGQ RALKNNEKES CTTKVRVVFK GADGVADMDT

AIGA
Length:454
Mass (Da):50,541
Last modified:June 12, 2007 - v2
Checksum:i5B725F292DB3576A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti189R → K in AAC27035 (PubMed:9624055).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051135 mRNA Translation: AAC27035.1
AC069326 Genomic DNA No translation available.
CP002688 Genomic DNA Translation: AED92667.1
AY050426 mRNA Translation: AAK91442.1
AY120692 mRNA Translation: AAM52235.1
AK226388 mRNA Translation: BAE98534.1
PIRiT52253
RefSeqiNP_568370.1, NM_121923.4
UniGeneiAt.21297

Genome annotation databases

EnsemblPlantsiAT5G19180.1; AT5G19180.1; AT5G19180
GeneIDi832038
GrameneiAT5G19180.1; AT5G19180.1; AT5G19180
KEGGiath:AT5G19180

Similar proteinsi

Entry informationi

Entry nameiUBA3_ARATH
AccessioniPrimary (citable) accession number: O65041
Secondary accession number(s): Q0WWG4, Q94A29
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: June 12, 2007
Last modified: April 25, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health