Reviewed, UniProtKB/Swiss-Prot O65020 (ETO1_ARATH)

Names and origin

Protein names

Recommended name:
    Ethylene-overproduction protein 1

Gene names

Name:	ETO1
Ordered Locus Names:	At3g51770
ORF Names:	ATEM1.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	951 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential regulator of the ethylene pathway, which acts by regulating the stability of 1-aminocyclopropane-1-carboxylate synthase (ACS) enzymes. May act as a substrate-specific adapter that connects ACS enzymes, such as ACS5, to ubiquitin ligase complexes, leading to proteasomal degradation of ACS enzymes. Ref.1
Subunit structure	Interacts with the C-terminal domain of ACS5. Interacts with CUL3. Putative component of a ubiquitin ligase complex containing CUL3. Ref.1
Domain	The BTB/POZ-like domain may mediate the interaction with some component of ubiquitin ligase complexes.
Sequence similarities	Belongs to the ETO1 family. Contains 1 BTB (POZ) domain. Contains 6 TPR repeats.

Ontologies

Keywords
Biological process	Ethylene signaling pathway Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat TPR repeat
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ethylene mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Ref.1 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ACS5	Q37001	2	EBI-593440,EBI-593450

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O65020-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 951	951	Ethylene-overproduction protein 1		PRO_0000106289
Regions
	Domain	242 – 342	101	BTB
	Repeat	443 – 476	34	TPR 1
	Repeat	572 – 605	34	TPR 2
	Repeat	698 – 731	34	TPR 3
	Repeat	772 – 805	34	TPR 4
	Repeat	868 – 901	34	TPR 5
	Repeat	903 – 934	32	TPR 6
	Coiled coil	815 – 854	40	Potential
	Compositional bias	23 – 29	7	Poly-Pro
	Compositional bias	30 – 50	21	Gly-rich
	Compositional bias	672 – 677	6	Poly-Leu
Experimental info
	Mutagenesis	466	1	F → I in eto1-5; induces overproduction of ethylene due to higher stability of ACS5.
	Sequence conflict	177 – 182	6	RFERRE → SSRGG in AAC14404. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 19, 2004. Version 2. Checksum: E275B8B440163B70	FASTA	951	107,040
	10 20 30 40 50 60 MRSLKLAEGC KGTQVYALNP SAPTPPPPPG NSSTGGGGGG GSGGGTGGVG DKLLQHLSDH 70 80 90 100 110 120 LRVNSVRSKS SRTYPPPTQP NAVVSPEFLL PCGLPVTDLL EPQIDPCLKF VDLVEKMAQV 130 140 150 160 170 180 YRRIENCSQF EKSGAYLEQC AIFRGISDPK LFRRSLRSSR QHAVDVHAKV VLASWLRFER 190 200 210 220 230 240 REDELIGTTS MDCCGRNLEC PKATLVSGYD PESVYDPCVC SGASRSEMMN EDECSTSQEV 250 260 270 280 290 300 DYDMSFCIGD EEVRCVRYKI ASLSRPFKAM LYGGFREMKR ATINFTQNGI SVEGMRAAEI 310 320 330 340 350 360 FSRTNRLDNF PPNVVLELLK LANRFCCDEL KSACDSHLAH LVNSLDEAML LIEYGLEEAA 370 380 390 400 410 420 YLLVAACLQV FLRELPSSMH NPNVIKIFCS AEGRERLASL GHASFTLYFF LSQIAMEDDM 430 440 450 460 470 480 KSNTTVMLLE RLVECAVDSW EKQLAYHQLG VVMLERKEYK DAQRWFNAAV EAGHLYSLVG 490 500 510 520 530 540 VARTKFKRDH RYSAYKIINS LISDHKATGW MHQERSLYCS GKEKLLDLDT ATEFDPTLTF 550 560 570 580 590 600 PYKFRAVALV EENQFGAAIA ELNKILGFKA SPDCLEMRAW ISIGMEDYEG ALKDIRALLT 610 620 630 640 650 660 LEPNFMMFNW KIHGDHMVEL LRPLAQQWSQ ADCWMQLYDR WSSVDDIGSL AVVHHMLAND 670 680 690 700 710 720 PGKSLLRFRQ SLLLLRLNCQ KAAMRSLRLA RNHSKSEHER LVYEGWILYD TGHREEALAK 730 740 750 760 770 780 AEESISIQRS FEAFFLKAYA LADSTLDPDS SNYVIQLLQE ALKCPSDGLR KGQALNNLGS 790 800 810 820 830 840 VYVDCEKLDL AADCYTNALT IKHTRAHQGL ARVYHLKNQR KAAYDEMTKL IEKAQNNASA 850 860 870 880 890 900 YEKRSEYCDR EMAQSDLCLA TQLDPLRTYP YRYRAAVLMD DHKESEAIDE LSRAISFKPD 910 920 930 940 950 LQLLHLRAAF YDSMGEGASA IKDCEAALCI DPGHADTLEL YHKAREPNDQ K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation of ethylene gas biosynthesis by the Arabidopsis ETO1 protein." Wang K.L.-C., Yoshida H., Lurin C., Ecker J.R. Nature 428:945-950(2004) [PubMed: 15118728] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH ACS5 AND CUL3, MUTANT ETO1-5.
[2]	"Fine sequence analysis of 60 kb around the Arabidopsis thaliana AtEm1 locus on chromosome III." Comella P., Wu H.-J., Laudie M., Berger C., Cooke R., Delseny M., Grellet F. Plant Mol. Biol. 41:687-700(1999) [PubMed: 10645728] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY572791 mRNA. Translation: AAT01656.1. AF049236 Genomic DNA. Translation: AAC14404.1. Different initiation.
IPI	IPI00657171.
PIR	T51148.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O65020. 1 interaction.
STRING	O65020.
Genome annotation databases
GenomeReviews	Gene locus AT3G51770 in contig BA000014_GR.
Organism-specific databases
TAIR	At3g51770.
Gene expression databases
ArrayExpress	O65020.
Genevestigator	O65020.
Family and domain databases
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR001440. TPR-1. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
Gene3D	G3DSA:3.30.710.10. BTB/POZ_fold. 1 hit. G3DSA:1.25.40.10. TPR-like_helical. 3 hits.
Pfam	PF00515. TPR_1. 3 hits. [Graphical view]
SMART	SM00225. BTB. 1 hit. SM00028. TPR. 5 hits. [Graphical view]
PROSITE	PS50097. BTB. False negative. PS50005. TPR. 4 hits. PS50293. TPR_REGION. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ETO1_ARATH

Accession

Primary (citable) accession number: O65020
Secondary accession number(s): Q6PWY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 19, 2004
Last modified:	November 24, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)