Reviewed, UniProtKB/Swiss-Prot O65015 (AMYB_TRIRP)

Names and origin

Protein names

Recommended name:
    Beta-amylase
    EC=3.2.1.2
Alternative name(s):
    1,4-alpha-D-glucan maltohydrolase

Gene names

Name:

BMY1

Organism

Trifolium repens (Creeping white clover)

Taxonomic identifier

3899 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Trifolieae › Trifolium

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.
Sequence similarities	Belongs to the glycosyl hydrolase 14 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-amylase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Beta-amylase		PRO_0000153939
Sites
	Active site	187	1	By similarity
	Active site	381	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O65015-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 8FC446AB7C927F19	FASTA	496	56,088
	10 20 30 40 50 60 MATSNKNMLL NYVPVYVMLP LGVINVNNVF EDPDGLKEQL VQLRAAGVDG VMVDVWWGII 70 80 90 100 110 120 EQKGPKEYDW SAYKSLFQLV QECGLKLQAI MSFHQCGGNV GDVVTIPIPQ WVLDIGESDP 130 140 150 160 170 180 DIFYTNRSGT RDKEYLTVGV DNKPIFHGRT AIEIYSDYMK SFRENMSEFL KSELIIDIEV 190 200 210 220 230 240 GLGPAGELRY PSYPQNQGWV FPGIGEFQCY DKYLKADFKA AAAKAGHSEW ELPDDAGTYN 250 260 270 280 290 300 DIPESTEFFK TNGTYLTEKG KFFLTWYSNQ LLNHGDQILD EANKAFLGCK VKLAIKVSGI 310 320 330 340 350 360 HWWYKAQNHA AELTAGYYNL DDRDGYRPIA KMVSRHHGIL NFTCLEMRDS EQSSDAQSAP 370 380 390 400 410 420 QELVQQVLSG GWRENIEVAG ENALSRYDAT AYNQIILNAR PQGVNKDGPP KLRMYGVTYL 430 440 450 460 470 480 RLSDDLLQES NFEIFKKFVV KMHADQSHCD DPQEYNHAIP PLKRSGPNIP VDDLLEATKP 490 ILPFPWDSET DMKVDG

References

[1]	"Nucleotide sequence of a beta-amylase cDNA from white clover stollons." Gallagher J., Gana J.A., Pollock C., Cunningham S.M., Volenec J.J. Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF049098 mRNA. Translation: AAD04259.1.
PIR	T08117.
3D structure databases
SMR	O65015. Positions 7-496.
ModBase	Search...
Protein family/group databases
CAZy	GH14. Glycoside Hydrolase Family 14.
Enzyme and pathway databases
BRENDA	3.2.1.2. 3928.
Family and domain databases
InterPro	IPR001554. Glyco_hydro_14. IPR018238. Glyco_hydro_14_CS. IPR001371. Glyco_hydro_14B_pln. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF01373. Glyco_hydro_14. 1 hit. [Graphical view]
PRINTS	PR00750. BETAAMYLASE. PR00842. GLHYDLASE14B.
PROSITE	PS00506. BETA_AMYLASE_1. 1 hit. PS00679. BETA_AMYLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMYB_TRIRP

Accession

Primary (citable) accession number: O65015

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	February 9, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)