Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytochrome P450 90B1

Gene

CYP90B1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the C22-alpha-hydroxylation step in brassinosteroids biosynthesis. Converts campestanol to 6-deoxocathasterone and 6-oxocampestanol to cathasterone.

Cofactori

hemeBy similarity

Pathwayi: brassinosteroid biosynthesis

This protein is involved in the pathway brassinosteroid biosynthesis, which is part of Plant hormone biosynthesis.
View all proteins of this organism that are known to be involved in the pathway brassinosteroid biosynthesis and in Plant hormone biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi462Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

  • brassinosteroid biosynthetic process Source: TAIR
  • brassinosteroid homeostasis Source: GO_Central
  • jasmonic acid mediated signaling pathway Source: TAIR
  • leaf development Source: TAIR
  • leaf shaping Source: TAIR
  • response to brassinosteroid Source: TAIR
  • response to jasmonic acid Source: TAIR
  • sterol metabolic process Source: GO_Central
  • unidimensional cell growth Source: TAIR

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processBrassinosteroid biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT3G50660-MONOMER
MetaCyc:AT3G50660-MONOMER
BRENDAi1.14.99.B1 399
ReactomeiR-ATH-211916 Vitamins
R-ATH-5365859 RA biosynthesis pathway
SABIO-RKiO64989
UniPathwayiUPA00381

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 90B1 (EC:1.14.-.-)
Alternative name(s):
Protein DWARF 4
Short name:
Dwarf4
Steroid 22-alpha-hydroxylase
Gene namesi
Name:CYP90B1
Synonyms:DWF4
Ordered Locus Names:At3g50660
ORF Names:T3A5.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G50660
TAIRilocus:2101704 AT3G50660

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei8 – 28HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi324 – 326Missing in dwf4-2; dwarf plant. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000521851 – 513Cytochrome P450 90B1Add BLAST513

Proteomic databases

PaxDbiO64989

Expressioni

Tissue specificityi

Expressed in stems, leaves, shoots, and roots, with a higher expression in siliques and apical shoots.1 Publication

Gene expression databases

ExpressionAtlasiO64989 baseline and differential
GenevisibleiO64989 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G50660.1

Structurei

3D structure databases

ProteinModelPortaliO64989
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi243 – 270Glu-richAdd BLAST28

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0157 Eukaryota
COG2124 LUCA
HOGENOMiHOG000237614
InParanoidiO64989
KOiK09587
OMAiGDFMQQH
OrthoDBiEOG093608UD

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

O64989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFETEHHTLL PLLLLPSLLS LLLFLILLKR RNRKTRFNLP PGKSGWPFLG
60 70 80 90 100
ETIGYLKPYT ATTLGDFMQQ HVSKYGKIYR SNLFGEPTIV SADAGLNRFI
110 120 130 140 150
LQNEGRLFEC SYPRSIGGIL GKWSMLVLVG DMHRDMRSIS LNFLSHARLR
160 170 180 190 200
TILLKDVERH TLFVLDSWQQ NSIFSAQDEA KKFTFNLMAK HIMSMDPGEE
210 220 230 240 250
ETEQLKKEYV TFMKGVVSAP LNLPGTAYHK ALQSRATILK FIERKMEERK
260 270 280 290 300
LDIKEEDQEE EEVKTEDEAE MSKSDHVRKQ RTDDDLLGWV LKHSNLSTEQ
310 320 330 340 350
ILDLILSLLF AGHETSSVAI ALAIFFLQAC PKAVEELREE HLEIARAKKE
360 370 380 390 400
LGESELNWDD YKKMDFTQCV INETLRLGNV VRFLHRKALK DVRYKGYDIP
410 420 430 440 450
SGWKVLPVIS AVHLDNSRYD QPNLFNPWRW QQQNNGASSS GSGSFSTWGN
460 470 480 490 500
NYMPFGGGPR LCAGSELAKL EMAVFIHHLV LKFNWELAED DKPFAFPFVD
510
FPNGLPIRVS RIL
Length:513
Mass (Da):58,868
Last modified:May 3, 2011 - v2
Checksum:iB1639BDD9A5D7C93
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti492K → Q in AAC05093 (PubMed:9490746).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044216 Genomic DNA Translation: AAC05093.1
AL132979 Genomic DNA Translation: CAB62435.1
CP002686 Genomic DNA Translation: AEE78691.1
AY090266 mRNA Translation: AAL90927.1
AF412114 mRNA Translation: AAL06567.1
PIRiT46143
RefSeqiNP_190635.1, NM_114926.4
UniGeneiAt.26401

Genome annotation databases

EnsemblPlantsiAT3G50660.1; AT3G50660.1; AT3G50660
GeneIDi824229
GrameneiAT3G50660.1; AT3G50660.1; AT3G50660
KEGGiath:AT3G50660

Similar proteinsi

Entry informationi

Entry nameiC90B1_ARATH
AccessioniPrimary (citable) accession number: O64989
Secondary accession number(s): Q9SCQ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: May 3, 2011
Last modified: April 25, 2018
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health