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Protein

Cytochrome P450 90B1

Gene

CYP90B1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the C22-alpha-hydroxylation step in brassinosteroids biosynthesis. Converts campestanol to 6-deoxocathasterone and 6-oxocampestanol to cathasterone.

Cofactori

hemeBy similarity

Pathwayi: brassinosteroid biosynthesis

This protein is involved in the pathway brassinosteroid biosynthesis, which is part of Plant hormone biosynthesis.
View all proteins of this organism that are known to be involved in the pathway brassinosteroid biosynthesis and in Plant hormone biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi462Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

  • brassinosteroid biosynthetic process Source: TAIR
  • brassinosteroid homeostasis Source: GO_Central
  • jasmonic acid mediated signaling pathway Source: TAIR
  • leaf development Source: TAIR
  • leaf shaping Source: TAIR
  • response to brassinosteroid Source: TAIR
  • response to jasmonic acid Source: TAIR
  • sterol metabolic process Source: GO_Central
  • unidimensional cell growth Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Brassinosteroid biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT3G50660-MONOMER.
MetaCyc:AT3G50660-MONOMER.
BRENDAi1.14.99.B1. 399.
ReactomeiR-ATH-211916. Vitamins.
R-ATH-5365859. RA biosynthesis pathway.
SABIO-RKO64989.
UniPathwayiUPA00381.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 90B1 (EC:1.14.-.-)
Alternative name(s):
Protein DWARF 4
Short name:
Dwarf4
Steroid 22-alpha-hydroxylase
Gene namesi
Name:CYP90B1
Synonyms:DWF4
Ordered Locus Names:At3g50660
ORF Names:T3A5.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G50660.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei8 – 28HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi324 – 326Missing in dwf4-2; dwarf plant. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000521851 – 513Cytochrome P450 90B1Add BLAST513

Proteomic databases

PaxDbiO64989.

Expressioni

Tissue specificityi

Expressed in stems, leaves, shoots, and roots, with a higher expression in siliques and apical shoots.1 Publication

Gene expression databases

GenevisibleiO64989. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G50660.1.

Structurei

3D structure databases

ProteinModelPortaliO64989.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi243 – 270Glu-richAdd BLAST28

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000237614.
InParanoidiO64989.
KOiK09587.
OMAiLEMAIFI.
OrthoDBiEOG093608UD.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O64989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFETEHHTLL PLLLLPSLLS LLLFLILLKR RNRKTRFNLP PGKSGWPFLG
60 70 80 90 100
ETIGYLKPYT ATTLGDFMQQ HVSKYGKIYR SNLFGEPTIV SADAGLNRFI
110 120 130 140 150
LQNEGRLFEC SYPRSIGGIL GKWSMLVLVG DMHRDMRSIS LNFLSHARLR
160 170 180 190 200
TILLKDVERH TLFVLDSWQQ NSIFSAQDEA KKFTFNLMAK HIMSMDPGEE
210 220 230 240 250
ETEQLKKEYV TFMKGVVSAP LNLPGTAYHK ALQSRATILK FIERKMEERK
260 270 280 290 300
LDIKEEDQEE EEVKTEDEAE MSKSDHVRKQ RTDDDLLGWV LKHSNLSTEQ
310 320 330 340 350
ILDLILSLLF AGHETSSVAI ALAIFFLQAC PKAVEELREE HLEIARAKKE
360 370 380 390 400
LGESELNWDD YKKMDFTQCV INETLRLGNV VRFLHRKALK DVRYKGYDIP
410 420 430 440 450
SGWKVLPVIS AVHLDNSRYD QPNLFNPWRW QQQNNGASSS GSGSFSTWGN
460 470 480 490 500
NYMPFGGGPR LCAGSELAKL EMAVFIHHLV LKFNWELAED DKPFAFPFVD
510
FPNGLPIRVS RIL
Length:513
Mass (Da):58,868
Last modified:May 3, 2011 - v2
Checksum:iB1639BDD9A5D7C93
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti492K → Q in AAC05093 (PubMed:9490746).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044216 Genomic DNA. Translation: AAC05093.1.
AL132979 Genomic DNA. Translation: CAB62435.1.
CP002686 Genomic DNA. Translation: AEE78691.1.
AY090266 mRNA. Translation: AAL90927.1.
AF412114 mRNA. Translation: AAL06567.1.
PIRiT46143.
RefSeqiNP_190635.1. NM_114926.4.
UniGeneiAt.26401.

Genome annotation databases

EnsemblPlantsiAT3G50660.1; AT3G50660.1; AT3G50660.
GeneIDi824229.
GrameneiAT3G50660.1; AT3G50660.1; AT3G50660.
KEGGiath:AT3G50660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044216 Genomic DNA. Translation: AAC05093.1.
AL132979 Genomic DNA. Translation: CAB62435.1.
CP002686 Genomic DNA. Translation: AEE78691.1.
AY090266 mRNA. Translation: AAL90927.1.
AF412114 mRNA. Translation: AAL06567.1.
PIRiT46143.
RefSeqiNP_190635.1. NM_114926.4.
UniGeneiAt.26401.

3D structure databases

ProteinModelPortaliO64989.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G50660.1.

Proteomic databases

PaxDbiO64989.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G50660.1; AT3G50660.1; AT3G50660.
GeneIDi824229.
GrameneiAT3G50660.1; AT3G50660.1; AT3G50660.
KEGGiath:AT3G50660.

Organism-specific databases

TAIRiAT3G50660.

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000237614.
InParanoidiO64989.
KOiK09587.
OMAiLEMAIFI.
OrthoDBiEOG093608UD.

Enzyme and pathway databases

UniPathwayiUPA00381.
BioCyciARA:AT3G50660-MONOMER.
MetaCyc:AT3G50660-MONOMER.
BRENDAi1.14.99.B1. 399.
ReactomeiR-ATH-211916. Vitamins.
R-ATH-5365859. RA biosynthesis pathway.
SABIO-RKO64989.

Miscellaneous databases

PROiO64989.

Gene expression databases

GenevisibleiO64989. AT.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC90B1_ARATH
AccessioniPrimary (citable) accession number: O64989
Secondary accession number(s): Q9SCQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: May 3, 2011
Last modified: November 30, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.