Reviewed, UniProtKB/Swiss-Prot O64967 (HMDH2_GOSHI)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase 2
      Short name=HMG-CoA reductase 2
    EC=1.1.1.34

Gene names

Name:

HMG2

Organism

Gossypium hirsutum (Upland cotton) (Gossypium mexicanum)

Taxonomic identifier

3635 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Malvales › Malvaceae › Malvoideae › Gossypium

Protein attributes

Sequence length	628 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP(+) = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein. Plastid membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Plastid
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: InterPro mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plastid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 628	628	3-hydroxy-3-methylglutaryl-coenzyme A reductase 2		PRO_0000114439
Regions
	Transmembrane	38 – 58	21	Potential
	Transmembrane	78 – 98	21	Potential
	Region	99 – 212	114	Linker By similarity
	Region	213 – 628	416	Catalytic By similarity
Sites
	Active site	307	1	Charge relay system By similarity
	Active site	439	1	Charge relay system By similarity
	Active site	515	1	Charge relay system By similarity
	Active site	613	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	153	1	N-linked (GlcNAc...) Potential
	Glycosylation	371	1	N-linked (GlcNAc...) Potential
	Glycosylation	484	1	N-linked (GlcNAc...) Potential
	Glycosylation	617	1	N-linked (GlcNAc...) Potential
	Glycosylation	625	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O64967-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: BF7704C30EA334F5	FASTA	628	67,603
	10 20 30 40 50 60 MEASRRSAVK PVIVLKPSKR FPLVEDTPGK ASDALPLPLY LTNAVFFTLF FTVVYFLLSR 70 80 90 100 110 120 WREKIRASIP LHAVTFPEIV AIFAFVASLI YLLGFFGIDF VQSLIIRPSG DVWSGEDYEE 130 140 150 160 170 180 ENEVLLHEED ARTVPCGQAL DCSVPSLPHM ARNVTAQRLF DEKPVRVATE EDARKVSCGQ 190 200 210 220 230 240 AVDCSLHSLP PRPPIVTSQK LFHEKTVIVT TEEDEEIIKS VVAGTLPSYS LESKLGDCKR 250 260 270 280 290 300 AAAIRREALQ RLTGRSLSGL PLDGFDYESI LGQCCEMPVG YVQIPVGIAG PLLLNGREYS 310 320 330 340 350 360 VPMATTEGCL VASTNRGCKA IHLSGGATSI LLKDGMTRAP VVRFSTAKRA AELKFYLEDP 370 380 390 400 410 420 ENFDTLAVVF NRSSRFGRLQ SIKCAIAGKN LYLRFTCSTG DAMGMNMVSK GVQNVLDFLQ 430 440 450 460 470 480 TDFPDMDVIG ISGNFCSDKK PAAVNWIEGR GKSVVCEAII EGDVVRKVLK TSVESLVELN 490 500 510 520 530 540 MLKNLTGSAM AGALGGFNAH ASNIVTAIYI ATGQDPAQNV ESSHCITMME AVNDGKDLHI 550 560 570 580 590 600 SVTMPSIEVG TVGGGTQLAS QSACLNLLGV KGASKDVAGA NSRMLATIVT GAVLAGELSL 610 620 MSALAAGQLV KSHMKYNRSS KDMSNLSS

References

[1]	"Two genomic clones encoding 3-hydroxy-3-methylglutaryl-coenzyme A reductase from cotton (Gossypium hirsutum L.)." Loguercio L.L., Wilkins T.A. (er) Plant Gene Register PGR98-031 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Acala SJ2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF038046 Genomic DNA. Translation: AAC05089.1.
PIR	T09785.
3D structure databases
HSSP	HSSP built from PDB template 1HWI based on UniProtKB P04035.
ModBase	Search...
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH2_GOSHI

Accession

Primary (citable) accession number: O64967

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 1, 1998
Last modified:	November 25, 2008
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)