Reviewed, UniProtKB/Swiss-Prot O64966 (HMDH1_GOSHI)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
      Short name=HMG-CoA reductase 1
    EC=1.1.1.34

Gene names

Name:

HMG1

Organism

Gossypium hirsutum (Upland cotton) (Gossypium mexicanum)

Taxonomic identifier

3635 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Malvales › Malvaceae › Malvoideae › Gossypium

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein. Plastid membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Plastid
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plastid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	3-hydroxy-3-methylglutaryl-coenzyme A reductase 1		PRO_0000114438
Regions
	Transmembrane	38 – 58	21	Potential
	Transmembrane	77 – 97	21	Potential
	Region	98 – 169	72	Linker By similarity
	Region	170 – 585	416	Catalytic By similarity
Sites
	Active site	264	1	Charge relay system By similarity
	Active site	396	1	Charge relay system By similarity
	Active site	472	1	Charge relay system By similarity
	Active site	570	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	328	1	N-linked (GlcNAc...) Potential
	Glycosylation	441	1	N-linked (GlcNAc...) Potential
	Glycosylation	574	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O64966-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 1350E7A9168EBE42	FASTA	585	62,835
	10 20 30 40 50 60 METHRRSSTN SIRSHKPARP IALEDDSTKA SDALPLPLYL TNAVFFTLFF SAVYFLLCRW 70 80 90 100 110 120 REKIRSSTPL HVVTFSEIVA ILASVASFIY LLGFFGIDFV QSLVLRPSAD VWATEDDEVE 130 140 150 160 170 180 SEVLLRNEDA RHVPCGQALD RSIRSLQPPE PIVTAEKVFD EMPVTVMTEE DEEIIRSVVC 190 200 210 220 230 240 GMTPSYSLES KLDDCKRAAA IRREALQRIT GKSLSGLPLD GFDYESILGQ CCEMPVGYEQ 250 260 270 280 290 300 IPVGIAGPLL LNGREYSVPM ATTEGCLVAS TNRGCKAIHL SGGATSVLLR DGMTRAPVVR 310 320 330 340 350 360 FGTAKRAADL KLYLEDPENF ETLACVFNRS SRFARLQSIK CAIAGKNLYL RFSCFTGDAM 370 380 390 400 410 420 GMNMVSKGVQ NVLDFLQTDF PDMDVIGISG NFCSDKKPAA VNWIEGRGKS VVCEAIINGD 430 440 450 460 470 480 VVTKVLKTSV ESLVELNMLK NLTGSAMAGA LGGFNAHASN IVTAVYIATG QDPAQNVESS 490 500 510 520 530 540 HCITMMEAVN GGKDLHVSVT MPSIEVGTVG GGTQLASQSA CLNLLGVKGA SKESPGANSI 550 560 570 580 LLATIVAGAV LAGELSLMSA LAAGQLVKSH MKYNRSSKDV SKVSS

References

[1]	"Two genomic clones encoding 3-hydroxy-3-methylglutaryl-coenzyme A reductase from cotton (Gossypium hirsutum L.)." Loguercio L.L., Wilkins T.A. Plant Gene Register PGR98-031 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Acala SJ2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF038045 Genomic DNA. Translation: AAC05088.1.
PIR	T09782.
3D structure databases
HSSP	HSSP built from PDB template 1HWI based on UniProtKB P04035.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.34. 1857.
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH1_GOSHI

Accession

Primary (citable) accession number: O64966

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)