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O64948 (LONP2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lon protease homolog 2, peroxisomal

EC=3.4.21.-
Gene names
Name:LON2
Ordered Locus Names:At5g47040
ORF Names:MQD22.18
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length888 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

ATP-dependent serine protease that mediates the selective degradation of misfolded and unassembled polypeptides in the peroxisomal matrix By similarity. Necessary for type 2 peroxisome targeting signal (PTS2)-containing protein processing and facilitates peroxisome matrix protein import. Ref.6

Subcellular location

Peroxisome matrix By similarity Ref.5.

Sequence similarities

Belongs to the peptidase S16 family.

Contains 1 Lon domain.

Sequence caution

The sequence AAO00734.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 888888Lon protease homolog 2, peroxisomal HAMAP-Rule MF_03121
PRO_0000026736

Regions

Domain11 – 253243Lon
Nucleotide binding408 – 4158ATP Potential
Motif886 – 8883Microbody targeting signal Potential

Sites

Active site7831 By similarity
Active site8261 By similarity

Sequences

Sequence LengthMass (Da)Tools
O64948 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: D7BAE741926A6F2F

FASTA88897,862
        10         20         30         40         50         60 
MAETVELPSR LAILPFRNKV LLPGAIIRIR CTSHSSVTLV EQELWQKEEK GLIGILPVRD 

        70         80         90        100        110        120 
DAEGSSIGTM INPGAGSDSG ERSLKFLVGT TDAQKSDAKD QQDLQWHTRG VAARALHLSR 

       130        140        150        160        170        180 
GVEKPSGRVT YVVVLEGLSR FNVQELGKRG PYSVARITSL EMTKAELEQV KQDPDFVALS 

       190        200        210        220        230        240 
RQFKTTAMEL VSVLEQKQKT GGRTKVLLET VPIHKLADIF VASFEMSFEE QLSMLDSVDL 

       250        260        270        280        290        300 
KVRLSKATEL VDRHLQSIRV AEKITQKVEG QLSKSQKEYL LRQQMRAIKE ELGDNDDDED 

       310        320        330        340        350        360 
DVAALERKMQ AAGMPSNIWK HAQRELRRLK KMQPQQPGYN SSRVYLELLA DLPWDKASEE 

       370        380        390        400        410        420 
HELDLKAAKE RLDSDHYGLA KVKQRIIEYL AVRKLKPDAR GPVLCFVGPP GVGKTSLASS 

       430        440        450        460        470        480 
IAAALGRKFV RLSLGGVKDE ADIRGHRRTY IGSMPGRLID GLKRVGVCNP VMLLDEIDKT 

       490        500        510        520        530        540 
GSDVRGDPAS ALLEVLDPEQ NKSFNDHYLN VPYDLSKVVF VATANRVQPI PPPLLDRMEL 

       550        560        570        580        590        600 
IELPGYTQEE KLKIAMRHLI PRVLDQHGLS SEFLKIPEAM VKNIIQRYTR EAGVRSLERN 

       610        620        630        640        650        660 
LAALARAAAV MVAEHEQSLP LSKDVQKLTS PLLNGRMAEG GEVEMEVIPM GVNDHEIGGT 

       670        680        690        700        710        720 
FQSPSALVVD ETMLEKILGP PRFDDSEAAD RVASAGVSVG LVWTTFGGEV QFVEATSMVG 

       730        740        750        760        770        780 
KGEMHLTGQL GDVIKESAQL ALTWVRARAS DFKLALAGDM NVLDGRDIHI HFPAGAVPKD 

       790        800        810        820        830        840 
GPSAGVTLVT ALVSLFSQKR VRADTAMTGE MTLRGLVLPV GGIKDKILAA HRYGIKRVIL 

       850        860        870        880 
PQRNSKDLVE VPAAVLSSLE VILAKRMEDV LENAFEGGCP WRNNYSKL 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and sequence analysis of a genomic clone of Arabidopsis thaliana encoding a LON protein."
Murray C., Christeller J.T., Gatehouse L.N., Laing W.A.
Plant Gene Register PGR98-023
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Landsberg erecta.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 317-888.
Strain: cv. Columbia.
[5]"In-depth proteome analysis of Arabidopsis leaf peroxisomes combined with in vivo subcellular targeting verification indicates novel metabolic and regulatory functions of peroxisomes."
Reumann S., Quan S., Aung K., Yang P., Manandhar-Shrestha K., Holbrook D., Linka N., Switzenberg R., Wilkerson C.G., Weber A.P., Olsen L.J., Hu J.
Plant Physiol. 150:125-143(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[6]"Arabidopsis LON2 is necessary for peroxisomal function and sustained matrix protein import."
Lingard M.J., Bartel B.
Plant Physiol. 151:1354-1365(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF033862 Genomic DNA. Translation: AAC05085.1.
AB013394 Genomic DNA. Translation: BAB10243.1.
CP002688 Genomic DNA. Translation: AED95460.1.
BT002374 mRNA. Translation: AAO00734.1. Different initiation.
BT010392 mRNA. Translation: AAQ56835.1.
RefSeqNP_568675.1. NM_124075.3.
UniGeneAt.8585.

3D structure databases

ProteinModelPortalO64948.
SMRO64948. Positions 93-296, 301-876.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid19998. 1 interaction.
STRING3702.AT5G47040.1-P.

Protein family/group databases

MEROPSS16.003.

Proteomic databases

PaxDbO64948.
PRIDEO64948.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G47040.1; AT5G47040.1; AT5G47040.
GeneID834750.
KEGGath:AT5G47040.

Organism-specific databases

GeneFarm1773. 137.
TAIRAT5G47040.

Phylogenomic databases

eggNOGCOG0466.
HOGENOMHOG000261408.
InParanoidO64948.
KOK01338.
OMASHIWEAI.
PhylomeDBO64948.

Enzyme and pathway databases

BioCycARA:AT5G47040-MONOMER.

Gene expression databases

GenevestigatorO64948.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_03121. lonp2_euk.
InterProIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR027501. Lonp2_euk.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR003111. Pept_S16_N.
IPR008268. Peptidase_S16_AS.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERPTHR10046. PTHR10046. 1 hit.
PfamPF00004. AAA. 1 hit.
PF02190. LON. 1 hit.
PF05362. Lon_C. 1 hit.
[Graphical view]
PIRSFPIRSF001174. Lon_proteas. 1 hit.
SMARTSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 2 hits.
TIGRFAMsTIGR00763. lon. 1 hit.
PROSITEPS01046. LON_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROO64948.

Entry information

Entry nameLONP2_ARATH
AccessionPrimary (citable) accession number: O64948
Secondary accession number(s): Q8GT60
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: May 14, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names