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Protein

Lon protease homolog 2, peroxisomal

Gene

LON2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

ATP-dependent serine protease that mediates the selective degradation of misfolded and unassembled polypeptides in the peroxisomal matrix (By similarity). Necessary for type 2 peroxisome targeting signal (PTS2)-containing protein processing and facilitates peroxisome matrix protein import.UniRule annotation1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei783 – 7831UniRule annotation
Active sitei826 – 8261UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi408 – 4158ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. ATP-dependent peptidase activity Source: UniProtKB-HAMAP
  3. serine-type endopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. growth Source: TAIR
  2. lateral root development Source: TAIR
  3. misfolded or incompletely synthesized protein catabolic process Source: UniProtKB-HAMAP
  4. protein import into peroxisome matrix, docking Source: TAIR
  5. protein processing Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G47040-MONOMER.
BRENDAi3.4.21.53. 399.

Protein family/group databases

MEROPSiS16.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Lon protease homolog 2, peroxisomalUniRule annotation (EC:3.4.21.-UniRule annotation)
Gene namesi
Name:LON2
Ordered Locus Names:At5g47040
ORF Names:MQD22.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G47040.

Subcellular locationi

  1. Peroxisome matrix UniRule annotation

GO - Cellular componenti

  1. organelle lumen Source: TAIR
  2. peroxisomal matrix Source: UniProtKB-SubCell
  3. peroxisome Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 888888Lon protease homolog 2, peroxisomalPRO_0000026736Add
BLAST

Proteomic databases

PaxDbiO64948.
PRIDEiO64948.

Expressioni

Gene expression databases

GenevestigatoriO64948.

Interactioni

Protein-protein interaction databases

BioGridi19998. 1 interaction.
STRINGi3702.AT5G47040.1-P.

Structurei

3D structure databases

ProteinModelPortaliO64948.
SMRiO64948. Positions 93-296, 301-876.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 253243LonUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi886 – 8883Microbody targeting signalUniRule annotation

Sequence similaritiesi

Belongs to the peptidase S16 family.UniRule annotation
Contains 1 Lon domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0466.
HOGENOMiHOG000261408.
InParanoidiO64948.
KOiK01338.
OMAiKVVKEHT.
PhylomeDBiO64948.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_03121. lonp2_euk.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR027501. Lonp2_euk.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR003111. Pept_S16_N.
IPR008268. Peptidase_S16_AS.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF02190. LON. 1 hit.
PF05362. Lon_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 2 hits.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS01046. LON_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O64948-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAETVELPSR LAILPFRNKV LLPGAIIRIR CTSHSSVTLV EQELWQKEEK
60 70 80 90 100
GLIGILPVRD DAEGSSIGTM INPGAGSDSG ERSLKFLVGT TDAQKSDAKD
110 120 130 140 150
QQDLQWHTRG VAARALHLSR GVEKPSGRVT YVVVLEGLSR FNVQELGKRG
160 170 180 190 200
PYSVARITSL EMTKAELEQV KQDPDFVALS RQFKTTAMEL VSVLEQKQKT
210 220 230 240 250
GGRTKVLLET VPIHKLADIF VASFEMSFEE QLSMLDSVDL KVRLSKATEL
260 270 280 290 300
VDRHLQSIRV AEKITQKVEG QLSKSQKEYL LRQQMRAIKE ELGDNDDDED
310 320 330 340 350
DVAALERKMQ AAGMPSNIWK HAQRELRRLK KMQPQQPGYN SSRVYLELLA
360 370 380 390 400
DLPWDKASEE HELDLKAAKE RLDSDHYGLA KVKQRIIEYL AVRKLKPDAR
410 420 430 440 450
GPVLCFVGPP GVGKTSLASS IAAALGRKFV RLSLGGVKDE ADIRGHRRTY
460 470 480 490 500
IGSMPGRLID GLKRVGVCNP VMLLDEIDKT GSDVRGDPAS ALLEVLDPEQ
510 520 530 540 550
NKSFNDHYLN VPYDLSKVVF VATANRVQPI PPPLLDRMEL IELPGYTQEE
560 570 580 590 600
KLKIAMRHLI PRVLDQHGLS SEFLKIPEAM VKNIIQRYTR EAGVRSLERN
610 620 630 640 650
LAALARAAAV MVAEHEQSLP LSKDVQKLTS PLLNGRMAEG GEVEMEVIPM
660 670 680 690 700
GVNDHEIGGT FQSPSALVVD ETMLEKILGP PRFDDSEAAD RVASAGVSVG
710 720 730 740 750
LVWTTFGGEV QFVEATSMVG KGEMHLTGQL GDVIKESAQL ALTWVRARAS
760 770 780 790 800
DFKLALAGDM NVLDGRDIHI HFPAGAVPKD GPSAGVTLVT ALVSLFSQKR
810 820 830 840 850
VRADTAMTGE MTLRGLVLPV GGIKDKILAA HRYGIKRVIL PQRNSKDLVE
860 870 880
VPAAVLSSLE VILAKRMEDV LENAFEGGCP WRNNYSKL
Length:888
Mass (Da):97,862
Last modified:August 1, 1998 - v1
Checksum:iD7BAE741926A6F2F
GO

Sequence cautioni

The sequence AAO00734.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033862 Genomic DNA. Translation: AAC05085.1.
AB013394 Genomic DNA. Translation: BAB10243.1.
CP002688 Genomic DNA. Translation: AED95460.1.
BT002374 mRNA. Translation: AAO00734.1. Different initiation.
BT010392 mRNA. Translation: AAQ56835.1.
RefSeqiNP_568675.1. NM_124075.3.
UniGeneiAt.8585.

Genome annotation databases

EnsemblPlantsiAT5G47040.1; AT5G47040.1; AT5G47040.
GeneIDi834750.
KEGGiath:AT5G47040.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033862 Genomic DNA. Translation: AAC05085.1.
AB013394 Genomic DNA. Translation: BAB10243.1.
CP002688 Genomic DNA. Translation: AED95460.1.
BT002374 mRNA. Translation: AAO00734.1. Different initiation.
BT010392 mRNA. Translation: AAQ56835.1.
RefSeqiNP_568675.1. NM_124075.3.
UniGeneiAt.8585.

3D structure databases

ProteinModelPortaliO64948.
SMRiO64948. Positions 93-296, 301-876.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19998. 1 interaction.
STRINGi3702.AT5G47040.1-P.

Protein family/group databases

MEROPSiS16.003.

Proteomic databases

PaxDbiO64948.
PRIDEiO64948.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G47040.1; AT5G47040.1; AT5G47040.
GeneIDi834750.
KEGGiath:AT5G47040.

Organism-specific databases

GeneFarmi1773. 137.
TAIRiAT5G47040.

Phylogenomic databases

eggNOGiCOG0466.
HOGENOMiHOG000261408.
InParanoidiO64948.
KOiK01338.
OMAiKVVKEHT.
PhylomeDBiO64948.

Enzyme and pathway databases

BioCyciARA:AT5G47040-MONOMER.
BRENDAi3.4.21.53. 399.

Miscellaneous databases

PROiO64948.

Gene expression databases

GenevestigatoriO64948.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_03121. lonp2_euk.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR027501. Lonp2_euk.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR003111. Pept_S16_N.
IPR008268. Peptidase_S16_AS.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF02190. LON. 1 hit.
PF05362. Lon_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 2 hits.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS01046. LON_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and sequence analysis of a genomic clone of Arabidopsis thaliana encoding a LON protein."
    Murray C., Christeller J.T., Gatehouse L.N., Laing W.A.
    Plant Gene Register PGR98-023
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Landsberg erecta.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
    Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
    DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 317-888.
    Strain: cv. Columbia.
  5. "In-depth proteome analysis of Arabidopsis leaf peroxisomes combined with in vivo subcellular targeting verification indicates novel metabolic and regulatory functions of peroxisomes."
    Reumann S., Quan S., Aung K., Yang P., Manandhar-Shrestha K., Holbrook D., Linka N., Switzenberg R., Wilkerson C.G., Weber A.P., Olsen L.J., Hu J.
    Plant Physiol. 150:125-143(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. "Arabidopsis LON2 is necessary for peroxisomal function and sustained matrix protein import."
    Lingard M.J., Bartel B.
    Plant Physiol. 151:1354-1365(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiLONP2_ARATH
AccessioniPrimary (citable) accession number: O64948
Secondary accession number(s): Q8GT60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: April 1, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.