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O64948

- LONP2_ARATH

UniProt

O64948 - LONP2_ARATH

Protein

Lon protease homolog 2, peroxisomal

Gene

LON2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    ATP-dependent serine protease that mediates the selective degradation of misfolded and unassembled polypeptides in the peroxisomal matrix By similarity. Necessary for type 2 peroxisome targeting signal (PTS2)-containing protein processing and facilitates peroxisome matrix protein import.1 PublicationUniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei783 – 7831UniRule annotation
    Active sitei826 – 8261UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi408 – 4158ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: RefGenome
    2. ATP-dependent peptidase activity Source: RefGenome
    3. sequence-specific DNA binding Source: RefGenome
    4. serine-type endopeptidase activity Source: RefGenome

    GO - Biological processi

    1. ATP catabolic process Source: RefGenome
    2. growth Source: TAIR
    3. lateral root development Source: TAIR
    4. misfolded or incompletely synthesized protein catabolic process Source: RefGenome
    5. protein import into peroxisome matrix, docking Source: TAIR
    6. protein processing Source: RefGenome

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G47040-MONOMER.

    Protein family/group databases

    MEROPSiS16.003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lon protease homolog 2, peroxisomalUniRule annotation (EC:3.4.21.-UniRule annotation)
    Gene namesi
    Name:LON2
    Ordered Locus Names:At5g47040
    ORF Names:MQD22.18
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G47040.

    Subcellular locationi

    Peroxisome matrix UniRule annotation

    GO - Cellular componenti

    1. nucleoid Source: RefGenome
    2. organelle lumen Source: TAIR
    3. peroxisomal matrix Source: RefGenome
    4. peroxisome Source: TAIR

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 888888Lon protease homolog 2, peroxisomalPRO_0000026736Add
    BLAST

    Proteomic databases

    PaxDbiO64948.
    PRIDEiO64948.

    Expressioni

    Gene expression databases

    GenevestigatoriO64948.

    Interactioni

    Protein-protein interaction databases

    BioGridi19998. 1 interaction.
    STRINGi3702.AT5G47040.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO64948.
    SMRiO64948. Positions 93-296, 301-876.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini11 – 253243LonUniRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi886 – 8883Microbody targeting signalUniRule annotation

    Sequence similaritiesi

    Belongs to the peptidase S16 family.UniRule annotation
    Contains 1 Lon domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0466.
    HOGENOMiHOG000261408.
    InParanoidiO64948.
    KOiK01338.
    OMAiSHIWEAI.
    PhylomeDBiO64948.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPiMF_03121. lonp2_euk.
    InterProiIPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR004815. Lon_bac/euk-typ.
    IPR027065. Lon_Prtase.
    IPR027501. Lonp2_euk.
    IPR027417. P-loop_NTPase.
    IPR008269. Pept_S16_C.
    IPR003111. Pept_S16_N.
    IPR008268. Peptidase_S16_AS.
    IPR015947. PUA-like_domain.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view]
    PANTHERiPTHR10046. PTHR10046. 1 hit.
    PfamiPF00004. AAA. 1 hit.
    PF02190. LON. 1 hit.
    PF05362. Lon_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001174. Lon_proteas. 1 hit.
    SMARTiSM00382. AAA. 1 hit.
    SM00464. LON. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    SSF54211. SSF54211. 1 hit.
    SSF88697. SSF88697. 2 hits.
    TIGRFAMsiTIGR00763. lon. 1 hit.
    PROSITEiPS01046. LON_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O64948-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAETVELPSR LAILPFRNKV LLPGAIIRIR CTSHSSVTLV EQELWQKEEK    50
    GLIGILPVRD DAEGSSIGTM INPGAGSDSG ERSLKFLVGT TDAQKSDAKD 100
    QQDLQWHTRG VAARALHLSR GVEKPSGRVT YVVVLEGLSR FNVQELGKRG 150
    PYSVARITSL EMTKAELEQV KQDPDFVALS RQFKTTAMEL VSVLEQKQKT 200
    GGRTKVLLET VPIHKLADIF VASFEMSFEE QLSMLDSVDL KVRLSKATEL 250
    VDRHLQSIRV AEKITQKVEG QLSKSQKEYL LRQQMRAIKE ELGDNDDDED 300
    DVAALERKMQ AAGMPSNIWK HAQRELRRLK KMQPQQPGYN SSRVYLELLA 350
    DLPWDKASEE HELDLKAAKE RLDSDHYGLA KVKQRIIEYL AVRKLKPDAR 400
    GPVLCFVGPP GVGKTSLASS IAAALGRKFV RLSLGGVKDE ADIRGHRRTY 450
    IGSMPGRLID GLKRVGVCNP VMLLDEIDKT GSDVRGDPAS ALLEVLDPEQ 500
    NKSFNDHYLN VPYDLSKVVF VATANRVQPI PPPLLDRMEL IELPGYTQEE 550
    KLKIAMRHLI PRVLDQHGLS SEFLKIPEAM VKNIIQRYTR EAGVRSLERN 600
    LAALARAAAV MVAEHEQSLP LSKDVQKLTS PLLNGRMAEG GEVEMEVIPM 650
    GVNDHEIGGT FQSPSALVVD ETMLEKILGP PRFDDSEAAD RVASAGVSVG 700
    LVWTTFGGEV QFVEATSMVG KGEMHLTGQL GDVIKESAQL ALTWVRARAS 750
    DFKLALAGDM NVLDGRDIHI HFPAGAVPKD GPSAGVTLVT ALVSLFSQKR 800
    VRADTAMTGE MTLRGLVLPV GGIKDKILAA HRYGIKRVIL PQRNSKDLVE 850
    VPAAVLSSLE VILAKRMEDV LENAFEGGCP WRNNYSKL 888
    Length:888
    Mass (Da):97,862
    Last modified:August 1, 1998 - v1
    Checksum:iD7BAE741926A6F2F
    GO

    Sequence cautioni

    The sequence AAO00734.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF033862 Genomic DNA. Translation: AAC05085.1.
    AB013394 Genomic DNA. Translation: BAB10243.1.
    CP002688 Genomic DNA. Translation: AED95460.1.
    BT002374 mRNA. Translation: AAO00734.1. Different initiation.
    BT010392 mRNA. Translation: AAQ56835.1.
    RefSeqiNP_568675.1. NM_124075.3.
    UniGeneiAt.8585.

    Genome annotation databases

    EnsemblPlantsiAT5G47040.1; AT5G47040.1; AT5G47040.
    GeneIDi834750.
    KEGGiath:AT5G47040.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF033862 Genomic DNA. Translation: AAC05085.1 .
    AB013394 Genomic DNA. Translation: BAB10243.1 .
    CP002688 Genomic DNA. Translation: AED95460.1 .
    BT002374 mRNA. Translation: AAO00734.1 . Different initiation.
    BT010392 mRNA. Translation: AAQ56835.1 .
    RefSeqi NP_568675.1. NM_124075.3.
    UniGenei At.8585.

    3D structure databases

    ProteinModelPortali O64948.
    SMRi O64948. Positions 93-296, 301-876.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 19998. 1 interaction.
    STRINGi 3702.AT5G47040.1-P.

    Protein family/group databases

    MEROPSi S16.003.

    Proteomic databases

    PaxDbi O64948.
    PRIDEi O64948.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G47040.1 ; AT5G47040.1 ; AT5G47040 .
    GeneIDi 834750.
    KEGGi ath:AT5G47040.

    Organism-specific databases

    GeneFarmi 1773. 137.
    TAIRi AT5G47040.

    Phylogenomic databases

    eggNOGi COG0466.
    HOGENOMi HOG000261408.
    InParanoidi O64948.
    KOi K01338.
    OMAi SHIWEAI.
    PhylomeDBi O64948.

    Enzyme and pathway databases

    BioCyci ARA:AT5G47040-MONOMER.

    Miscellaneous databases

    PROi O64948.

    Gene expression databases

    Genevestigatori O64948.

    Family and domain databases

    Gene3Di 3.30.230.10. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPi MF_03121. lonp2_euk.
    InterProi IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR004815. Lon_bac/euk-typ.
    IPR027065. Lon_Prtase.
    IPR027501. Lonp2_euk.
    IPR027417. P-loop_NTPase.
    IPR008269. Pept_S16_C.
    IPR003111. Pept_S16_N.
    IPR008268. Peptidase_S16_AS.
    IPR015947. PUA-like_domain.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view ]
    PANTHERi PTHR10046. PTHR10046. 1 hit.
    Pfami PF00004. AAA. 1 hit.
    PF02190. LON. 1 hit.
    PF05362. Lon_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001174. Lon_proteas. 1 hit.
    SMARTi SM00382. AAA. 1 hit.
    SM00464. LON. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    SSF54211. SSF54211. 1 hit.
    SSF88697. SSF88697. 2 hits.
    TIGRFAMsi TIGR00763. lon. 1 hit.
    PROSITEi PS01046. LON_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and sequence analysis of a genomic clone of Arabidopsis thaliana encoding a LON protein."
      Murray C., Christeller J.T., Gatehouse L.N., Laing W.A.
      Plant Gene Register PGR98-023
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Landsberg erecta.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
      Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
      DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 317-888.
      Strain: cv. Columbia.
    5. "In-depth proteome analysis of Arabidopsis leaf peroxisomes combined with in vivo subcellular targeting verification indicates novel metabolic and regulatory functions of peroxisomes."
      Reumann S., Quan S., Aung K., Yang P., Manandhar-Shrestha K., Holbrook D., Linka N., Switzenberg R., Wilkerson C.G., Weber A.P., Olsen L.J., Hu J.
      Plant Physiol. 150:125-143(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    6. "Arabidopsis LON2 is necessary for peroxisomal function and sustained matrix protein import."
      Lingard M.J., Bartel B.
      Plant Physiol. 151:1354-1365(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiLONP2_ARATH
    AccessioniPrimary (citable) accession number: O64948
    Secondary accession number(s): Q8GT60
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3