O64900 (C80B2_ESCCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
EC=1.14.13.71

Alternative name(s):
Cytochrome P450 80B2

Gene names

Name:

CYP80B2

Organism

Eschscholzia californica (California poppy)

Taxonomic identifier

3467 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › Ranunculales › Papaveraceae › Eschscholzioideae › Eschscholzia

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	3'-hydroxylation of (S)-N-methylcoclaurine.
Catalytic activity	(S)-N-methylcoclaurine + NADPH + O2 = (S)-3'-hydroxy-N-methylcoclaurine + NADP+ + H2O.
Cofactor	Heme group.
Pathway	Alkaloid biosynthesis; (S)-reticuline biosynthesis; (S)-reticuline from (S)-norcoclaurine: step 3/4.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein Potential. Microsome membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-methylcoclaurine 3'-monooxygenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2		PRO_0000052158
Regions
	Transmembrane	3 – 23	21	Helical; Potential
Sites
	Metal binding	430	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O64900 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 5DBFFECBAAC1A3F2	FASTA	488	54,896
	10 20 30 40 50 60 MEVVTVALIA VIISSILYLL FGSSGHKNLP PGPKPWPIVG NLLQLGEKPH AQFAELAQTY 70 80 90 100 110 120 GDIFTLKMGT ETVVVASTSS AASEILKTHD RILSARYVFQ SFRVKGHVEN SIVWSDCTET 130 140 150 160 170 180 WKNLRKVCRT ELFTQKMIES QAHVREKKCE EMVEYLMKKQ GEEVKIVEVI FGTLVNIFGN 190 200 210 220 230 240 LIFSQNIFEL GXPNSGSSEF KEYLWRMLEL GNSTNPADYF PMLGKFDLFG QRKEVAECLK 250 260 270 280 290 300 GIYAIWGAML QERKLAKKVD GYQSKNDFVD VCLDSGLNDY QINALLMELF GAGTETSAST 310 320 330 340 350 360 IEWAMTELTK NPKITAKLRS ELQTVVGERS VKESDFPNLP YLEATVKETL RLHPPTPLLL 370 380 390 400 410 420 PRRALETCTI LNYTIPKDCQ IMVNAWGIGR DPKTWIDPLT FSPERFLNSS VDFRGNDFSL 430 440 450 460 470 480 IPFGAGRRIC PGLPIANQFI ALLVATFVQN LDWCLPNGMS VDHLIVEEKF GLTLQKEPPL FIVPKSRV

References

[1]

"Molecular cloning and functional heterologous expression of two alleles encoding (S)-N-methylcoclaurine 3'-hydroxylase (CYP80B1), a new methyl jasmonate-inducible cytochrome P-450-dependent mono-oxygenase of benzylisoquinoline alkaloid biosynthesis." Pauli H.H., Kutchan T.M. Plant J. 13:793-801(1998) [PubMed: 9681018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF014801 mRNA. Translation: AAC39453.1.
PIR	T07963.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C80B2_ESCCA

Accession

Primary (citable) accession number: O64900

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	June 28, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program