Reviewed, UniProtKB/Swiss-Prot O64899 (C80B1_ESCCA)

Names and origin

Protein names

Recommended name:
    (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1
    EC=1.14.13.71
Alternative name(s):
    Cytochrome P450 80B1

Gene names

Name:

CYP80B1

Organism

Eschscholzia californica (California poppy)

Taxonomic identifier

3467 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › Ranunculales › Papaveraceae › Eschscholzioideae › Eschscholzia

Protein attributes

Sequence length	487 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	3'-hydroxylation of (S)-N-methylcoclaurine.
Catalytic activity	(S)-N-methylcoclaurine + NADPH + O2 = (S)-3'-hydroxy-N-methylcoclaurine + NADP+ + H2O.
Cofactor	Heme group.
Pathway	Alkaloid biosynthesis; (S)-reticuline biosynthesis; (S)-reticuline from (S)-norcoclaurine: step 3/4.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein Potential. Microsome membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-methylcoclaurine 3'-monooxygenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 487	›487	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1		PRO_0000052157
Regions
	Transmembrane	4 – 24	21	Potential
Sites
	Metal binding	429	1	Iron (heme axial ligand) By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	O64899-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 2EF90A9B67500AE2	FASTA	487	54,644
	10 20 30 40 50 60 GTSTVALIAV IISSILYLLF GGSGHKNLPP GPKPWPIVGN LLQLGEKPHA QFAELAQTYG 70 80 90 100 110 120 DIFTLKMGTE TVVVASTSSA ASEILKTHDR ILSARYVFQS FRVKGHVENS IVWSDCTETW 130 140 150 160 170 180 KNLRKVCRTE LFTQKMIESQ AHVREKKCEE MVEYLMKKQG EEVKIVEVIF GTLVNIFGNL 190 200 210 220 230 240 IFSQNIFELG DPNSGSSEFK EYLWRMLELG NSTNPADYFP MLGKFDLFGQ RKEVAECLKG 250 260 270 280 290 300 IYAIWGAMLQ ERKLAKKVDG YKSKNDFVDV CLDSGLNDYQ INALLMELFG AGTETSASTI 310 320 330 340 350 360 EWAMTELTKN PKITAKIRSE IQTVVGERSV KESDFPNLPY LEATVKETLR LHPPTPLLLP 370 380 390 400 410 420 RRALETCTIL NYTIPKDCQI MVNAWGIGRD PKTWTDPLTF SPERFLNSSV DFRGNDFSLI 430 440 450 460 470 480 PFGAGRRICP GLPIANQFIA LLVATFVQNL DWCLPNGMSV DHLIVEEKFG LTLQKEPPLF IVPKSRV

References

[1]

"Molecular cloning and functional heterologous expression of two alleles encoding (S)-N-methylcoclaurine 3'-hydroxylase (CYP80B1), a new methyl jasmonate-inducible cytochrome P-450-dependent mono-oxygenase of benzylisoquinoline alkaloid biosynthesis." Pauli H.H., Kutchan T.M. Plant J. 13:793-801(1998) [PubMed: 9681018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF014800 mRNA. Translation: AAC39452.1.
PIR	T07960.
3D structure databases
HSSP	HSSP built from PDB template 1DT6 based on UniProtKB P00179.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.13.71. 2644.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C80B1_ESCCA

Accession

Primary (citable) accession number: O64899

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)