ID   GUN13_ARATH             Reviewed;         490 AA.
AC   O64890;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   24-JAN-2024, entry version 137.
DE   RecName: Full=Endoglucanase 13;
DE            EC=3.2.1.4;
DE   AltName: Full=Endo-1,4-beta glucanase 13;
DE   Flags: Precursor;
GN   OrderedLocusNames=At2g44550; ORFNames=F4I1.55;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY.
RX   PubMed=15170254; DOI=10.1007/s00239-003-2571-x;
RA   Libertini E., Li Y., McQueen-Mason S.J.;
RT   "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family.";
RL   J. Mol. Evol. 58:506-515(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR   EMBL; AC003672; AAC27457.1; -; Genomic_DNA.
DR   EMBL; AC004521; AAM14964.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC10437.1; -; Genomic_DNA.
DR   PIR; T02411; T02411.
DR   RefSeq; NP_181983.1; NM_130019.1.
DR   AlphaFoldDB; O64890; -.
DR   SMR; O64890; -.
DR   STRING; 3702.O64890; -.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   PaxDb; 3702-AT2G44550-1; -.
DR   ProteomicsDB; 247265; -.
DR   EnsemblPlants; AT2G44550.1; AT2G44550.1; AT2G44550.
DR   GeneID; 819063; -.
DR   Gramene; AT2G44550.1; AT2G44550.1; AT2G44550.
DR   KEGG; ath:AT2G44550; -.
DR   Araport; AT2G44550; -.
DR   TAIR; AT2G44550; GH9B10.
DR   eggNOG; ENOG502QRF6; Eukaryota.
DR   HOGENOM; CLU_008926_1_4_1; -.
DR   InParanoid; O64890; -.
DR   OMA; WTSTICI; -.
DR   OrthoDB; 1347382at2759; -.
DR   PhylomeDB; O64890; -.
DR   BioCyc; ARA:AT2G44550-MONOMER; -.
DR   PRO; PR:O64890; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O64890; baseline.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR   PANTHER; PTHR22298:SF125; ENDOGLUCANASE 12-RELATED; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS60032; GH9_1; 1.
DR   PROSITE; PS00592; GH9_2; 1.
DR   Genevisible; O64890; AT.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW   Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..490
FT                   /note="Endoglucanase 13"
FT                   /id="PRO_0000249265"
FT   ACT_SITE        86
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT   ACT_SITE        412
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        464
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        473
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   CARBOHYD        6
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   490 AA;  53448 MW;  70A6A9155FAFFC08 CRC64;
     MSQLKNGSSQ CLWTSICIVL IVMSMAREAV STNYAEALKN SLLYFEAQRS GKLPPNQRVT
     WRGDSALRDG SDAHIDLTGG YYDAGDNMKF GFPLAFTTTM LAWSNIEMAS QLRAHHEKGN
     ALRALKWATD YLIKAHPQPN VLYGQVGEGN SDHKCWMRPE DMTTPRTSYR IDAQHPGSDL
     AGETAAAMAA ASIAFAPSDK AYANILIGHA KDLFAFAKAH RGLYQNSIPN AGGFYASSGY
     EDELLWAAAW LHRATNDQIY LDYLTEAETG GPRTVFAWDD KFVGAQVLVA KLALEGKVES
     SEQIVEYKSM AEQFICNCAQ KGDNNVKKTP GGLLYFLPWN NLQYTTAATF VLSAYSKYLE
     AAKASIDCPD GALQASDLLQ VARSQVDYIL GSNPQKMSYM VGVGTNYPKK PHHRAASIVS
     IRQDKTPVTC SGGYDKWYNN PAPNPNVLAG AVVGGPDDND VYGDERSNFQ QAEPATVTTA
     PLVGVLALVF
//