Reviewed, UniProtKB/Swiss-Prot O64890 (GUN13_ARATH)

Names and origin

Protein names

Recommended name:
    Endoglucanase 13
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta glucanase 13

Gene names

Ordered Locus Names:	At2g44550
ORF Names:	F4I1.55

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 490	464	Endoglucanase 13		PRO_0000249265
Sites
	Active site	412	1	By similarity
	Active site	464	1	By similarity
	Active site	473	1	By similarity
Amino acid modifications
	Glycosylation	6	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O64890-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 70A6A9155FAFFC08	FASTA	490	53,448
	10 20 30 40 50 60 MSQLKNGSSQ CLWTSICIVL IVMSMAREAV STNYAEALKN SLLYFEAQRS GKLPPNQRVT 70 80 90 100 110 120 WRGDSALRDG SDAHIDLTGG YYDAGDNMKF GFPLAFTTTM LAWSNIEMAS QLRAHHEKGN 130 140 150 160 170 180 ALRALKWATD YLIKAHPQPN VLYGQVGEGN SDHKCWMRPE DMTTPRTSYR IDAQHPGSDL 190 200 210 220 230 240 AGETAAAMAA ASIAFAPSDK AYANILIGHA KDLFAFAKAH RGLYQNSIPN AGGFYASSGY 250 260 270 280 290 300 EDELLWAAAW LHRATNDQIY LDYLTEAETG GPRTVFAWDD KFVGAQVLVA KLALEGKVES 310 320 330 340 350 360 SEQIVEYKSM AEQFICNCAQ KGDNNVKKTP GGLLYFLPWN NLQYTTAATF VLSAYSKYLE 370 380 390 400 410 420 AAKASIDCPD GALQASDLLQ VARSQVDYIL GSNPQKMSYM VGVGTNYPKK PHHRAASIVS 430 440 450 460 470 480 IRQDKTPVTC SGGYDKWYNN PAPNPNVLAG AVVGGPDDND VYGDERSNFQ QAEPATVTTA 490 PLVGVLALVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family." Libertini E., Li Y., McQueen-Mason S.J. J. Mol. Evol. 58:506-515(2004) [PubMed: 15170254] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC003672 Genomic DNA. Translation: AAC27457.1. AC004521 Genomic DNA. Translation: AAM14964.1.
IPI	IPI00534177.
PIR	T02411.
RefSeq	NP_181983.1.
UniGene	At.53122
3D structure databases
HSSP	HSSP built from PDB template 1KS8 based on UniProtKB O77044.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Proteomic databases
PRIDE	O64890.
Genome annotation databases
GeneID	819063.
GenomeReviews	Gene locus AT2G44550 in contig CT485783_GR.
KEGG	ath:AT2G44550.
NMPDR	fig|3702.1.peg.11631.
Organism-specific databases
TAIR	At2g44550.
Phylogenomic databases
OMA	LYQNSIP.
Enzyme and pathway databases
BRENDA	3.2.1.4. 302.
Gene expression databases
Genevestigator	O64890.
GermOnline	AT2G44550. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN13_ARATH

Accession

Primary (citable) accession number: O64890

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)