Reviewed, UniProtKB/Swiss-Prot O64889 (GUN12_ARATH)

Names and origin

Protein names

Recommended name:
    Endoglucanase 12
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta glucanase 12

Gene names

Ordered Locus Names:	At2g44540
ORF Names:	F4I1.52

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 491	460	Endoglucanase 12		PRO_0000249264
Sites
	Active site	413	1	By similarity
	Active site	465	1	By similarity
	Active site	474	1	By similarity
Amino acid modifications
	Glycosylation	6	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O64889-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: E8C02220EA89635B	FASTA	491	53,421
	10 20 30 40 50 60 MSQSKNGSSQ CLWTSICIVV LVMSMARGAV STNYAEALQK SLLYFEAQRS GKLPPNQKVT 70 80 90 100 110 120 WRGDSALKDG SDAHIDLTGG YYDAGDNMKF GFPLAFTTTM LAWSNIEMGS ELRAHHEQGN 130 140 150 160 170 180 SLRALKWATD YLIKAHPQPN VLYGQVGEGN SDHKCWMRPE DMTTPRTSYR IDAQHPGSDL 190 200 210 220 230 240 AGETAAAMAA ASIAFAPFDK AYANILIGHA KDLFAFAKAH PGLYQNSITN AGGFYASSGY 250 260 270 280 290 300 EDELLWAAAW LHRATNDQIY LNYLTQASGT GGPRSVFAWD DKFVGAQVLM AKLALERKVG 310 320 330 340 350 360 SNGKIAEYKS MAEQFICNCA QKGSNNVKKT PGGLLYFLPW NNLQYTTTAS FVLSAYSKYL 370 380 390 400 410 420 TKAKASIQCP KGALQASDLL QIARSQVDYI LGSNPQKMSY MVGVGTNYPK KPHHRAASIV 430 440 450 460 470 480 SIRKDKTPVT CSGGYDKWYN NPAPNPNVLM GALVGGPNEN DVYGDERSNF QQAEPATVTV 490 APFVGVLAAV F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family." Libertini E., Li Y., McQueen-Mason S.J. J. Mol. Evol. 58:506-515(2004) [PubMed: 15170254] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC003672 Genomic DNA. Translation: AAC27456.1. AC004521 Genomic DNA. Translation: AAM14965.1.
IPI	IPI00528397.
PIR	T02410.
RefSeq	NP_181982.1.
UniGene	At.12528
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Proteomic databases
PRIDE	O64889.
Genome annotation databases
GeneID	819062.
GenomeReviews	Gene locus AT2G44540 in contig CT485783_GR.
KEGG	ath:AT2G44540.
NMPDR	fig|3702.1.peg.11630.
Organism-specific databases
TAIR	At2g44540.
Phylogenomic databases
eggNOG	euNOG10137.
HOGENOM	HBG746347.
InParanoid	O64889.
OMA	NEVANDY.
Enzyme and pathway databases
BRENDA	3.2.1.4. 302.
Gene expression databases
ArrayExpress	O64889.
Genevestigator	O64889.
GermOnline	AT2G44540. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN12_ARATH

Accession

Primary (citable) accession number: O64889

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	August 1, 1998
Last modified:	February 9, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)