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Protein

Endoglucanase 12

Gene

At2g44540

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei413 – 4131PROSITE-ProRule annotation
Active sitei465 – 4651PROSITE-ProRule annotation
Active sitei474 – 4741PROSITE-ProRule annotation

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
  2. cell wall organization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciARA:AT2G44540-MONOMER.

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 12 (EC:3.2.1.4)
Alternative name(s):
Endo-1,4-beta glucanase 12
Gene namesi
Ordered Locus Names:At2g44540
ORF Names:F4I1.52
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G44540.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence AnalysisAdd
BLAST
Chaini32 – 491460Endoglucanase 12PRO_0000249264Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi6 – 61N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO64889.
PRIDEiO64889.

Expressioni

Gene expression databases

GenevestigatoriO64889.

Interactioni

Protein-protein interaction databases

IntActiO64889. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO64889.
SMRiO64889. Positions 33-488.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG05134.
HOGENOMiHOG000021033.
InParanoidiO64889.
OMAiPDENDYY.
PhylomeDBiO64889.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O64889-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQSKNGSSQ CLWTSICIVV LVMSMARGAV STNYAEALQK SLLYFEAQRS
60 70 80 90 100
GKLPPNQKVT WRGDSALKDG SDAHIDLTGG YYDAGDNMKF GFPLAFTTTM
110 120 130 140 150
LAWSNIEMGS ELRAHHEQGN SLRALKWATD YLIKAHPQPN VLYGQVGEGN
160 170 180 190 200
SDHKCWMRPE DMTTPRTSYR IDAQHPGSDL AGETAAAMAA ASIAFAPFDK
210 220 230 240 250
AYANILIGHA KDLFAFAKAH PGLYQNSITN AGGFYASSGY EDELLWAAAW
260 270 280 290 300
LHRATNDQIY LNYLTQASGT GGPRSVFAWD DKFVGAQVLM AKLALERKVG
310 320 330 340 350
SNGKIAEYKS MAEQFICNCA QKGSNNVKKT PGGLLYFLPW NNLQYTTTAS
360 370 380 390 400
FVLSAYSKYL TKAKASIQCP KGALQASDLL QIARSQVDYI LGSNPQKMSY
410 420 430 440 450
MVGVGTNYPK KPHHRAASIV SIRKDKTPVT CSGGYDKWYN NPAPNPNVLM
460 470 480 490
GALVGGPNEN DVYGDERSNF QQAEPATVTV APFVGVLAAV F
Length:491
Mass (Da):53,421
Last modified:August 1, 1998 - v1
Checksum:iE8C02220EA89635B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003672 Genomic DNA. Translation: AAC27456.1.
AC004521 Genomic DNA. Translation: AAM14965.1.
CP002685 Genomic DNA. Translation: AEC10436.1.
PIRiT02410.
RefSeqiNP_181982.1. NM_130018.2.
UniGeneiAt.12528.

Genome annotation databases

EnsemblPlantsiAT2G44540.1; AT2G44540.1; AT2G44540.
GeneIDi819062.
KEGGiath:AT2G44540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003672 Genomic DNA. Translation: AAC27456.1.
AC004521 Genomic DNA. Translation: AAM14965.1.
CP002685 Genomic DNA. Translation: AEC10436.1.
PIRiT02410.
RefSeqiNP_181982.1. NM_130018.2.
UniGeneiAt.12528.

3D structure databases

ProteinModelPortaliO64889.
SMRiO64889. Positions 33-488.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO64889. 1 interaction.

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Proteomic databases

PaxDbiO64889.
PRIDEiO64889.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G44540.1; AT2G44540.1; AT2G44540.
GeneIDi819062.
KEGGiath:AT2G44540.

Organism-specific databases

TAIRiAT2G44540.

Phylogenomic databases

eggNOGiNOG05134.
HOGENOMiHOG000021033.
InParanoidiO64889.
OMAiPDENDYY.
PhylomeDBiO64889.

Enzyme and pathway databases

BioCyciARA:AT2G44540-MONOMER.

Gene expression databases

GenevestigatoriO64889.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family."
    Libertini E., Li Y., McQueen-Mason S.J.
    J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.

Entry informationi

Entry nameiGUN12_ARATH
AccessioniPrimary (citable) accession number: O64889
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: August 1, 1998
Last modified: January 7, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.