O64888 (KPRS5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase 5, chloroplastic
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase 5

Gene names

Name:	PRS5
Ordered Locus Names:	At2g44530
ORF Names:	F16B22.2

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.
Sequence caution	The sequence AAC27455.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM14963.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O64888-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 33	33	Chloroplast Potential
	Chain	34 – 394	361	Ribose-phosphate pyrophosphokinase 5, chloroplastic		PRO_0000141096
Regions
	Region	288 – 303	16	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	202	1	Magnesium Potential
	Metal binding	204	1	Magnesium Potential
	Metal binding	213	1	Magnesium Potential
	Metal binding	217	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 29, 2005. Version 2. Checksum: 022EFEF64AF12D60	FASTA	394	42,625
	10 20 30 40 50 60 MASIVQPSPT FPALNLRRSS LIRPPSSVRF PLKCNAADPY KFDGGNSAGF HLLTGDTVPA 70 80 90 100 110 120 SFSRTRLEDS IYQNTTRLRI FSGTANPILA QEISCYLGLD LGKIKIKRFA DGEIYVQLQE 130 140 150 160 170 180 SVRGCDVFLV QPTCPPANEN LMELLVMIDA CRRASAKTIT AVIPYFGYAR ADRKTQGRES 190 200 210 220 230 240 IAAKLVANLI TQSGADRVLA CDLHSGQSMG YFDIPVDHVY GQPVILDYLA SKAISSEDLV 250 260 270 280 290 300 VVSPDVGGVA RARAFAKKLS DAPLAIVDKR RHGHNVAEVM NLIGDVKGKV AIMVDDMIDT 310 320 330 340 350 360 AGTISKGAAL LHQEGAREVY ACTTHAVFSP PAISRLSSGL FQEVIITNTI PLSEKNYFPQ 370 380 390 LTVLSVANLL GETIWRVHDD CSGAIEPFST LGID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC003672 Genomic DNA. Translation: AAC27455.1. Sequence problems. AC004521 Genomic DNA. Translation: AAM14963.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC10434.1. AY127024 mRNA. Translation: AAM83248.1. BT001041 mRNA. Translation: AAN46795.1.
IPI	IPI00547336.
PIR	T02409.
RefSeq	NP_181981.2. NM_130017.4.
UniGene	At.27497. At.43497. At.73198.
3D structure databases
ProteinModelPortal	O64888.
SMR	O64888. Positions 73-388.
ModBase	Search...
Protein-protein interaction databases
IntAct	O64888. 1 interaction.
STRING	O64888.
Proteomic databases
PRIDE	O64888.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G44530.1; AT2G44530.1; AT2G44530.
GeneID	819061.
GenomeReviews	Gene locus AT2G44530 in contig CT485783_GR.
KEGG	ath:AT2G44530.
NMPDR	fig|3702.1.peg.11629.
Organism-specific databases
GeneFarm	4839. 468.
TAIR	At2g44530.
Phylogenomic databases
GeneTree	EPGT00070000029078.
HOGENOM	HBG519284.
InParanoid	O64888.
OMA	TRISPTE.
PhylomeDB	O64888.
ProtClustDB	PLN02369.
Gene expression databases
ArrayExpress	O64888.
Genevestigator	O64888.
GermOnline	AT2G44530. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000842. PRib_PP_synth_CS. IPR000836. PRibTrfase. IPR005946. Rib-P_diPkinase. [Graphical view]
KO	K00948.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. RibP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS5_ARATH

Accession

Primary (citable) accession number: O64888
Secondary accession number(s): Q8L7T8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	March 29, 2005
Last modified:	November 16, 2011
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program