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Protein

Beta-glucosidase 15

Gene

BGLU15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Beta-glucosidase involved in the rapid degradation of flavonol 3-O-beta-glucoside-7-O-alpha-rhamnosides during abiotic stress recovery. No activity with quercetin 3-O-alpha-rhamnoside, quercetin 3-O-beta-galactoside and rutin.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Kineticsi

kcat is 1.30 sec(-1) with kaempferol 3-O-beta-glucoside-7-O-alpha-rhamnoside as substrate. kcat is 1.10 sec(-1) with quercetin 3-O-beta-glucoside-7-O-alpha-rhamnoside as substrate. kcat is 0.92 sec(-1) with kaempferol 3-O-beta-glucoside as substrate. kcat is 0.57 sec(-1) with quercetin 3-O-beta-glucoside as substrate. kcat is 4.08 sec(-1) with p-nitrophenyl beta-D-glucoside as substrate.1 Publication
  1. KM=51 µM for kaempferol 3-O-beta-glucoside-7-O-alpha-rhamnoside1 Publication
  2. KM=36 µM for quercetin 3-O-beta-glucoside-7-O-alpha-rhamnoside1 Publication
  3. KM=60 µM for kaempferol 3-O-beta-glucoside1 Publication
  4. KM=52 µM for quercetin 3-O-beta-glucoside1 Publication
  5. KM=2592 µM for p-nitrophenyl beta-D-glucoside1 Publication
  1. Vmax=1.08 µmol/min/mg enzyme with kaempferol 3-O-beta-glucoside-7-O-alpha-rhamnoside as substrate1 Publication
  2. Vmax=0.89 µmol/min/mg enzyme with quercetin 3-O-beta-glucoside-7-O-alpha-rhamnoside as substrate1 Publication
  3. Vmax=0.76 µmol/min/mg enzyme with kaempferol 3-O-beta-glucoside as substrate1 Publication
  4. Vmax=0.47 µmol/min/mg enzyme with quercetin 3-O-beta-glucoside as substrate1 Publication
  5. Vmax=3.37 µmol/min/mg enzyme with p-nitrophenyl beta-D-glucoside as substrate1 Publication

pH dependencei

Optimum pH is 5.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50SubstrateBy similarity1
Binding sitei154SubstrateBy similarity1
Binding sitei199SubstrateBy similarity1
Active sitei200Proton donorBy similarity1
Binding sitei343SubstrateBy similarity1
Active sitei413NucleophileBy similarity1
Binding sitei458SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT2G44450-MONOMER
ReactomeiR-ATH-189085 Digestion of dietary carbohydrate

Protein family/group databases

CAZyiGH1 Glycoside Hydrolase Family 1

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 15 (EC:3.2.1.21)
Short name:
AtBGLU15
Gene namesi
Name:BGLU15
Ordered Locus Names:At2g44450
ORF Names:F4I1.26
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G44450
TAIRilocus:2050605 AT2G44450

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000038957823 – 506Beta-glucosidase 15Add BLAST484

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi24N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi25N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi81N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi219 ↔ 227By similarity
Glycosylationi226N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO64879
PRIDEiO64879

Expressioni

Inductioni

Up-regulated during abiotic stress recovery.1 Publication

Gene expression databases

ExpressionAtlasiO64879 baseline and differential
GenevisibleiO64879 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G44450.1

Structurei

3D structure databases

ProteinModelPortaliO64879
SMRiO64879
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni465 – 466Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0626 Eukaryota
COG2723 LUCA
HOGENOMiHOG000088630
InParanoidiO64879
KOiK01188
OMAiIKPFATM
OrthoDBiEOG093606W7
PhylomeDBiO64879

Family and domain databases

InterProiView protein in InterPro
IPR001360 Glyco_hydro_1
IPR033132 Glyco_hydro_1_N_CS
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR10353 PTHR10353, 1 hit
PfamiView protein in Pfam
PF00232 Glyco_hydro_1, 1 hit
PRINTSiPR00131 GLHYDRLASE1
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00653 GLYCOSYL_HYDROL_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O64879-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGNYLSLLV VLIVLASNDV LANNNSSTPK LRRSDFPEDF IFGSATSAYQ
60 70 80 90 100
VEGGAHEDGR GPSIWDTFSE KYPEKIKDGS NGSVADNSYH LYKEDVALLH
110 120 130 140 150
QIGFNAYRFS ISWSRILPRG NLKGGINQAG IDYYNNLINE LLSKGIKPFA
160 170 180 190 200
TMFHWDTPQA LEDAYGGFRG AEIVNDFRDY ADICFKNFGD RVKHWMTLNE
210 220 230 240 250
PLTVVQQGYV AGVMAPGRCS KFTNPNCTDG NGATEPYIVG HNLILSHGAA
260 270 280 290 300
VQVYREKYKA SQQGQVGIAL NAGWNLPYTE SPKDRLAAAR AMAFTFDYFM
310 320 330 340 350
EPLVTGKYPV DMVNNVKGRL PIFTAQQSKM LKGSYDFIGI NYYSSTYAKD
360 370 380 390 400
VPCSTKDVTM FSDPCASVTG ERDGVPIGPK AASDWLLIYP KGIRDLVLYA
410 420 430 440 450
KYKFKDPVMY ITENGRDEFS TNKIFLKDGD RIDYYARHLE MVQDAISVGA
460 470 480 490 500
NVKGFFAWSL LDNFEWAMGY TVRFGLVYVD FKDGCKRYPK KSAEWFRKLL

NEKKND
Length:506
Mass (Da):56,905
Last modified:August 1, 1998 - v1
Checksum:i82FDFB3B52946DF9
GO

Sequence cautioni

The sequence BX818939 differs from that shown. Sequencing errors.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004521 Genomic DNA Translation: AAC16091.1
CP002685 Genomic DNA Translation: AEC10421.1
BX818939 mRNA No translation available.
PIRiT02400
RefSeqiNP_181973.1, NM_130008.3
UniGeneiAt.43824

Genome annotation databases

EnsemblPlantsiAT2G44450.1; AT2G44450.1; AT2G44450
GeneIDi819052
GrameneiAT2G44450.1; AT2G44450.1; AT2G44450
KEGGiath:AT2G44450

Similar proteinsi

Entry informationi

Entry nameiBGL15_ARATH
AccessioniPrimary (citable) accession number: O64879
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health