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Protein

Casein kinase II subunit alpha-3

Gene

CKA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha chain contains the catalytic site. The tetrameric holoenzyme CK2 is composed of two alpha and two beta subunits (By similarity). Acts as circadian clock component that maintains the correct period length through phosphorylation of CCA1 (PubMed:21900482).By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei63 – 631ATPPROSITE-ProRule annotation
Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi40 – 489ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • chromatin organization Source: TAIR
  • circadian rhythm Source: TAIR
  • DNA repair Source: TAIR
  • regulation of cell cycle Source: TAIR
  • regulation of response to DNA damage stimulus Source: TAIR
  • response to gamma radiation Source: TAIR
  • response to UV-C Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G23080-MONOMER.
ARA:GQT-273-MONOMER.
ReactomeiR-ATH-2514853. Condensation of Prometaphase Chromosomes.
R-ATH-6804756. Regulation of TP53 Activity through Phosphorylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase II subunit alpha-3Curated (EC:2.7.11.1)
Short name:
CK II
Gene namesi
Name:CKA3Curated
Ordered Locus Names:At2g23080
ORF Names:F21P24.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G23080.

Subcellular locationi

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but the triple mutant cka1, cka2 and cka3 show altered circadian rythms and delayed flowering under long day conditions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 333333Casein kinase II subunit alpha-3PRO_0000085897Add
BLAST

Proteomic databases

PaxDbiO64817.
PRIDEiO64817.

PTM databases

iPTMnetiO64817.

Expressioni

Gene expression databases

GenevisibleiO64817. AT.

Interactioni

Subunit structurei

Heterotetramer of two catalytic alpha subunits and two regulatory beta subunits.Curated

Binary interactionsi

WithEntry#Exp.IntActNotes
CAM4P258542EBI-1235683,EBI-1235664

Protein-protein interaction databases

BioGridi2191. 1 interaction.
IntActiO64817. 8 interactions.
STRINGi3702.AT2G23080.1.

Structurei

3D structure databases

ProteinModelPortaliO64817.
SMRiO64817. Positions 2-326.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 319286Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0668. Eukaryota.
ENOG410XNPP. LUCA.
HOGENOMiHOG000233021.
InParanoidiO64817.
KOiK03097.
OMAiQCRMMST.
OrthoDBiEOG09360EPL.
PhylomeDBiO64817.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O64817-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSKARVYTDV NVVRPKEYWD YESLVVQWGH QDDYEVVRKV GRGKYSEVFE
60 70 80 90 100
GKNVNTNERC VIKILKPVKK KKIKREIKIL QNLCGGPNIV KLYDIVRDEH
110 120 130 140 150
SKTPSLVFEF VNSVDFKVLY PTLTDYDIRY YIYELLKALD FCHSQGIMHR
160 170 180 190 200
DVKPHNVMID HQLRKLRLID WGLAEFYHPG KEYNVRVASR YFKGPELLVD
210 220 230 240 250
LQDYDYSLDM WSLGCMFAGM IFRKEPFFYG HDNHDQLVKI AKVLGTNELD
260 270 280 290 300
HYLNKYQLDL DPQLEALVGR HVPKPWSKFI NADNQHLVSP EAIDFLDKLL
310 320 330
QYDHQDRLTA REAMDHPYFA QVKAAESSRL RTQ
Note: No experimental confirmation available.
Length:333
Mass (Da):39,309
Last modified:August 1, 1998 - v1
Checksum:iB84D4168F3AC127A
GO
Isoform 2 (identifier: O64817-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     292-307: AIDFLDKLLQYDHQDR → VLLFFEKHSFASFWVY
     308-333: Missing.

Note: No experimental confirmation available.
Show »
Length:307
Mass (Da):36,380
Checksum:i67B40FA91C5E7358
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei292 – 30716AIDFL…DHQDR → VLLFFEKHSFASFWVY in isoform 2. 1 PublicationVSP_043761Add
BLAST
Alternative sequencei308 – 33326Missing in isoform 2. 1 PublicationVSP_043762Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004401 Genomic DNA. Translation: AAC17824.1.
CP002685 Genomic DNA. Translation: AEC07405.1.
CP002685 Genomic DNA. Translation: AEC07406.1.
AY035088 mRNA. Translation: AAK59593.1.
AY062954 mRNA. Translation: AAL33786.1.
BX819262 mRNA. No translation available.
AY087736 mRNA. Translation: AAM65273.1.
PIRiC84620.
RefSeqiNP_179890.1. NM_127872.3. [O64817-1]
NP_973518.1. NM_201789.2. [O64817-2]
UniGeneiAt.46799.
At.66416.

Genome annotation databases

EnsemblPlantsiAT2G23080.1; AT2G23080.1; AT2G23080. [O64817-1]
GeneIDi816838.
KEGGiath:AT2G23080.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004401 Genomic DNA. Translation: AAC17824.1.
CP002685 Genomic DNA. Translation: AEC07405.1.
CP002685 Genomic DNA. Translation: AEC07406.1.
AY035088 mRNA. Translation: AAK59593.1.
AY062954 mRNA. Translation: AAL33786.1.
BX819262 mRNA. No translation available.
AY087736 mRNA. Translation: AAM65273.1.
PIRiC84620.
RefSeqiNP_179890.1. NM_127872.3. [O64817-1]
NP_973518.1. NM_201789.2. [O64817-2]
UniGeneiAt.46799.
At.66416.

3D structure databases

ProteinModelPortaliO64817.
SMRiO64817. Positions 2-326.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2191. 1 interaction.
IntActiO64817. 8 interactions.
STRINGi3702.AT2G23080.1.

PTM databases

iPTMnetiO64817.

Proteomic databases

PaxDbiO64817.
PRIDEiO64817.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G23080.1; AT2G23080.1; AT2G23080. [O64817-1]
GeneIDi816838.
KEGGiath:AT2G23080.

Organism-specific databases

TAIRiAT2G23080.

Phylogenomic databases

eggNOGiKOG0668. Eukaryota.
ENOG410XNPP. LUCA.
HOGENOMiHOG000233021.
InParanoidiO64817.
KOiK03097.
OMAiQCRMMST.
OrthoDBiEOG09360EPL.
PhylomeDBiO64817.

Enzyme and pathway databases

BioCyciARA:AT2G23080-MONOMER.
ARA:GQT-273-MONOMER.
ReactomeiR-ATH-2514853. Condensation of Prometaphase Chromosomes.
R-ATH-6804756. Regulation of TP53 Activity through Phosphorylation.

Miscellaneous databases

PROiO64817.

Gene expression databases

GenevisibleiO64817. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCSK23_ARATH
AccessioniPrimary (citable) accession number: O64817
Secondary accession number(s): F4ILF5, Q8L5T7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.