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Protein

Casein kinase II subunit alpha-4, chloroplastic

Gene

CKA4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha chain contains the catalytic site (By similarity). Involved in the regulation of various developmental processes (PubMed:26025542). Involved in the regulation of plant growth and flowering time (PubMed:26883224). Involved in retrograde signaling in plant responses to abscisic acid (ABA) and heat stress. May act as an enhancing factor in abiotic stress signaling through modulation of the expression of some molecular players in retrograde signaling (PubMed:24803505). Phosphorylates RuBisCo activase (RCA) at Thr-78 (PubMed:27064346).By similarity4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei161 – 1611ATPPROSITE-ProRule annotation
Active sitei249 – 2491Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi138 – 1469ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G23070-MONOMER.
ReactomeiR-ATH-2514853. Condensation of Prometaphase Chromosomes.
R-ATH-6804756. Regulation of TP53 Activity through Phosphorylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase II subunit alpha-4, chloroplasticCurated (EC:2.7.11.1)
Short name:
CK2-alpha41 Publication
Alternative name(s):
Plastid-targeted casein kinase 2 alpha1 Publication
Short name:
cpCK2alpha1 Publication
Gene namesi
Name:CKA41 Publication
Ordered Locus Names:At2g23070
ORF Names:F21P24.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G23070.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: UniProtKB-SubCell
  • cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Defects in root, hypocotyl, cotyledon and leaf development. Delayed flowering. Reduced growth, delayed flowering and hyperaccumulation of anthocyanins.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5555ChloroplastSequence analysisAdd
BLAST
Chaini56 – 432377Casein kinase II subunit alpha-4, chloroplasticPRO_0000417493Add
BLAST

Proteomic databases

PaxDbiO64816.
PRIDEiO64816.

PTM databases

iPTMnetiO64816.

Expressioni

Tissue specificityi

Expressed in root tips, lateral root primordia, cotyledons, leaf primordia, sepals, filaments, stigma, and anthers.1 Publication

Gene expression databases

GenevisibleiO64816. AT.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.Curated

Protein-protein interaction databases

BioGridi2190. 12 interactions.
IntActiO64816. 8 interactions.
MINTiMINT-8294383.
STRINGi3702.AT2G23070.1.

Structurei

3D structure databases

ProteinModelPortaliO64816.
SMRiO64816. Positions 67-424.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini132 – 417286Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi61 – 7515Gln-richAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0668. Eukaryota.
ENOG410XNPP. LUCA.
HOGENOMiHOG000233021.
InParanoidiO64816.
KOiK03097.
OMAiVQDDYEV.
OrthoDBiEOG09360EPL.
PhylomeDBiO64816.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O64816-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALRPCTGFT ISSLRNASAA NNNLFSLLSF SSSSPAKRNL LLSSLQDNLR
60 70 80 90 100
RFASSASLYR QHLRNQQQQH QQQQQSRVKE KSETLAQKIG KSIRRAGAPS
110 120 130 140 150
KARVYADVNV VRPKDYWDYE SLAVQWGVQD DYEVVRKVGR GKYSEVFEGI
160 170 180 190 200
HATDNEKCVI KILKPVKKKK IKREIKILQN LCGGPNIVKL LDIVRDQQSK
210 220 230 240 250
TPSLIFEHVN NKDFKVLYPT LSDYDVRYYI FELLKALDFC HSRGIMHRDV
260 270 280 290 300
KPHNVMIDHE QRKLRLIDWG LAEFYHPGKE YNVRVASRYF KGPELLVDLQ
310 320 330 340 350
DYDYSLDLWS LGCMFAGMIF RKEPFFYGHD NYDQLVKIAK VLGTDELNAY
360 370 380 390 400
LNKYRIELDP NLTSLVGRHS RKPWTKFINS ENQHLAVPEA VDFVDKLLRY
410 420 430
DHQERPTAKE AMAHPYFYPI RNAESSRTPR SQ
Length:432
Mass (Da):50,236
Last modified:August 1, 1998 - v1
Checksum:i6DFCF7453B225E4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004401 Genomic DNA. Translation: AAC17823.1.
CP002685 Genomic DNA. Translation: AEC07404.1.
AY062631 mRNA. Translation: AAL32709.1.
AY081478 mRNA. Translation: AAM10040.1.
AY536852 mRNA. Translation: AAS65790.1.
PIRiB84620.
RefSeqiNP_179889.1. NM_127871.4.
UniGeneiAt.26849.

Genome annotation databases

EnsemblPlantsiAT2G23070.1; AT2G23070.1; AT2G23070.
GeneIDi816837.
GrameneiAT2G23070.1; AT2G23070.1; AT2G23070.
KEGGiath:AT2G23070.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004401 Genomic DNA. Translation: AAC17823.1.
CP002685 Genomic DNA. Translation: AEC07404.1.
AY062631 mRNA. Translation: AAL32709.1.
AY081478 mRNA. Translation: AAM10040.1.
AY536852 mRNA. Translation: AAS65790.1.
PIRiB84620.
RefSeqiNP_179889.1. NM_127871.4.
UniGeneiAt.26849.

3D structure databases

ProteinModelPortaliO64816.
SMRiO64816. Positions 67-424.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2190. 12 interactions.
IntActiO64816. 8 interactions.
MINTiMINT-8294383.
STRINGi3702.AT2G23070.1.

PTM databases

iPTMnetiO64816.

Proteomic databases

PaxDbiO64816.
PRIDEiO64816.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G23070.1; AT2G23070.1; AT2G23070.
GeneIDi816837.
GrameneiAT2G23070.1; AT2G23070.1; AT2G23070.
KEGGiath:AT2G23070.

Organism-specific databases

TAIRiAT2G23070.

Phylogenomic databases

eggNOGiKOG0668. Eukaryota.
ENOG410XNPP. LUCA.
HOGENOMiHOG000233021.
InParanoidiO64816.
KOiK03097.
OMAiVQDDYEV.
OrthoDBiEOG09360EPL.
PhylomeDBiO64816.

Enzyme and pathway databases

BioCyciARA:AT2G23070-MONOMER.
ReactomeiR-ATH-2514853. Condensation of Prometaphase Chromosomes.
R-ATH-6804756. Regulation of TP53 Activity through Phosphorylation.

Miscellaneous databases

PROiO64816.

Gene expression databases

GenevisibleiO64816. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCSK2P_ARATH
AccessioniPrimary (citable) accession number: O64816
Secondary accession number(s): Q6QJ31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.