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Protein

Casein kinase II subunit alpha-4, chloroplastic

Gene

CKA4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha chain contains the catalytic site (By similarity). Involved in the regulation of various developmental processes (PubMed:26025542). Involved in the regulation of plant growth and flowering time (PubMed:26883224). Involved in retrograde signaling in plant responses to abscisic acid (ABA) and heat stress. May act as an enhancing factor in abiotic stress signaling through modulation of the expression of some molecular players in retrograde signaling (PubMed:24803505). Phosphorylates RuBisCo activase (RCA) at Thr-78 (PubMed:27064346).By similarity4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei161ATPPROSITE-ProRule annotation1
Active sitei249Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi138 – 146ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: UniProtKB
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  • chloroplast-nucleus signaling pathway Source: UniProtKB
  • regulation of growth Source: UniProtKB
  • regulation of photoperiodism, flowering Source: UniProtKB

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-2514853 Condensation of Prometaphase Chromosomes
R-ATH-6804756 Regulation of TP53 Activity through Phosphorylation

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase II subunit alpha-4, chloroplasticCurated (EC:2.7.11.1)
Short name:
CK2-alpha41 Publication
Alternative name(s):
Plastid-targeted casein kinase 2 alpha1 Publication
Short name:
cpCK2alpha1 Publication
Gene namesi
Name:CKA41 Publication
Ordered Locus Names:At2g23070
ORF Names:F21P24.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G23070
TAIRilocus:2045339 AT2G23070

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Defects in root, hypocotyl, cotyledon and leaf development. Delayed flowering. Reduced growth, delayed flowering and hyperaccumulation of anthocyanins.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 55ChloroplastSequence analysisAdd BLAST55
ChainiPRO_000041749356 – 432Casein kinase II subunit alpha-4, chloroplasticAdd BLAST377

Proteomic databases

PaxDbiO64816
PRIDEiO64816

PTM databases

iPTMnetiO64816

Expressioni

Tissue specificityi

Expressed in root tips, lateral root primordia, cotyledons, leaf primordia, sepals, filaments, stigma, and anthers.1 Publication

Gene expression databases

ExpressionAtlasiO64816 baseline and differential
GenevisibleiO64816 AT

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.Curated

Protein-protein interaction databases

BioGridi2190, 12 interactors
IntActiO64816, 8 interactors
MINTiO64816
STRINGi3702.AT2G23070.1

Structurei

3D structure databases

ProteinModelPortaliO64816
SMRiO64816
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini132 – 417Protein kinasePROSITE-ProRule annotationAdd BLAST286

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi61 – 75Gln-richAdd BLAST15

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0668 Eukaryota
ENOG410XNPP LUCA
HOGENOMiHOG000233021
InParanoidiO64816
KOiK03097
OMAiCFKGPEL
OrthoDBiEOG09360EPL
PhylomeDBiO64816

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O64816-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALRPCTGFT ISSLRNASAA NNNLFSLLSF SSSSPAKRNL LLSSLQDNLR
60 70 80 90 100
RFASSASLYR QHLRNQQQQH QQQQQSRVKE KSETLAQKIG KSIRRAGAPS
110 120 130 140 150
KARVYADVNV VRPKDYWDYE SLAVQWGVQD DYEVVRKVGR GKYSEVFEGI
160 170 180 190 200
HATDNEKCVI KILKPVKKKK IKREIKILQN LCGGPNIVKL LDIVRDQQSK
210 220 230 240 250
TPSLIFEHVN NKDFKVLYPT LSDYDVRYYI FELLKALDFC HSRGIMHRDV
260 270 280 290 300
KPHNVMIDHE QRKLRLIDWG LAEFYHPGKE YNVRVASRYF KGPELLVDLQ
310 320 330 340 350
DYDYSLDLWS LGCMFAGMIF RKEPFFYGHD NYDQLVKIAK VLGTDELNAY
360 370 380 390 400
LNKYRIELDP NLTSLVGRHS RKPWTKFINS ENQHLAVPEA VDFVDKLLRY
410 420 430
DHQERPTAKE AMAHPYFYPI RNAESSRTPR SQ
Length:432
Mass (Da):50,236
Last modified:August 1, 1998 - v1
Checksum:i6DFCF7453B225E4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004401 Genomic DNA Translation: AAC17823.1
CP002685 Genomic DNA Translation: AEC07404.1
AY062631 mRNA Translation: AAL32709.1
AY081478 mRNA Translation: AAM10040.1
AY536852 mRNA Translation: AAS65790.1
PIRiB84620
RefSeqiNP_179889.1, NM_127871.5
UniGeneiAt.26849

Genome annotation databases

EnsemblPlantsiAT2G23070.1; AT2G23070.1; AT2G23070
GeneIDi816837
GrameneiAT2G23070.1; AT2G23070.1; AT2G23070
KEGGiath:AT2G23070

Similar proteinsi

Entry informationi

Entry nameiCSK2P_ARATH
AccessioniPrimary (citable) accession number: O64816
Secondary accession number(s): Q6QJ31
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health