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Protein

Serine carboxypeptidase-like 9

Gene

SCPL9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of 1,2-bis-O-sinapoyl beta-D-glucoside and an unidentified compound 1.1 Publication

Catalytic activityi

2 1-O-sinapoyl beta-D-glucoside = D-glucose + 1,2-bis-O-sinapoyl beta-D-glucoside.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei175 – 1751By similarity
Active sitei362 – 3621By similarity
Active sitei415 – 4151By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT2G23010-MONOMER.
ARA:GQT-492-MONOMER.
MetaCyc:AT2G23010-MONOMER.

Protein family/group databases

ESTHERiarath-SCP9. Carboxypeptidase_S10.
MEROPSiS10.A12.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine carboxypeptidase-like 9
Alternative name(s):
Sinapoylglucose--sinapoylglucose O-sinapoyltransferase (EC:2.3.1.103)
Short name:
SST
Sinapoylglucose--sinapoylglucose acyltransferase
Gene namesi
Name:SCPL9
Synonyms:SST
Ordered Locus Names:At2g23010
ORF Names:F21P24.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G23010.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Decreased levels of 1,2-disinapoyl-glucose due to a partial redundancy with SCPL8 and SCPL13.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 437416Serine carboxypeptidase-like 9PRO_0000274623Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi101 – 1011N-linked (GlcNAc...)Sequence analysis
Disulfide bondi243 ↔ 257By similarity
Disulfide bondi281 ↔ 293By similarity
Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence analysis
Glycosylationi346 – 3461N-linked (GlcNAc...)Sequence analysis
Glycosylationi378 – 3781N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO64811.
PRIDEiO64811.

Expressioni

Tissue specificityi

Expressed in seedlings, leaves, flowers and siliques.1 Publication

Gene expression databases

ExpressionAtlasiO64811. baseline and differential.
GenevisibleiO64811. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G23010.1.

Structurei

3D structure databases

ProteinModelPortaliO64811.
SMRiO64811. Positions 25-437.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1282. Eukaryota.
COG2939. LUCA.
HOGENOMiHOG000198297.
InParanoidiO64811.
KOiK16296.
PhylomeDBiO64811.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O64811-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLILKFMLL ILLVSSHHVR SGSIVKFLPG FKGPLPFELE TGYIGIGEEE
60 70 80 90 100
NVQFFYYFIK SDKNPQEDPL IIWLNGGPGC SCLSGLFFEN GPLALKNKVY
110 120 130 140 150
NGSVPSLVST TYSWTKTANI IFLDQPVGSG FSYSKTPIER TSDTSEVKKI
160 170 180 190 200
HEFLQKWLIK HPQFLSNPFY VVGDSYSGMI VPALVHEISK GNYICCNPPI
210 220 230 240 250
NLQGYVLGNP ITHIEFEQNF RIPYAHGMSL ISDELYESLK RICKGNYFSV
260 270 280 290 300
DPSNKKCLKL VEEYHKCTDN INSHHTLIAN CDDSNTQHIS PDCYYYPYHL
310 320 330 340 350
VECWANNESV REALHVDKGS IGEWIRDHRG IPYKSDIRSS IPYHMNNSIN
360 370 380 390 400
GYRSLIFSGD HDITMPFQAT QAWIKSLNYS IIDDWRPWMI KGQIAGYTRT
410 420 430
YSNKMTFATV KGGGHTAEYL PEESSIMFQR WISGQPL
Length:437
Mass (Da):49,756
Last modified:August 1, 1998 - v1
Checksum:i9E976FDB66E204B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004401 Genomic DNA. Translation: AAC17818.1.
CP002685 Genomic DNA. Translation: AEC07397.1.
PIRiE84619.
RefSeqiNP_179884.1. NM_127866.2. [O64811-1]
UniGeneiAt.39356.

Genome annotation databases

EnsemblPlantsiAT2G23010.1; AT2G23010.1; AT2G23010. [O64811-1]
GeneIDi816832.
KEGGiath:AT2G23010.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004401 Genomic DNA. Translation: AAC17818.1.
CP002685 Genomic DNA. Translation: AEC07397.1.
PIRiE84619.
RefSeqiNP_179884.1. NM_127866.2. [O64811-1]
UniGeneiAt.39356.

3D structure databases

ProteinModelPortaliO64811.
SMRiO64811. Positions 25-437.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G23010.1.

Protein family/group databases

ESTHERiarath-SCP9. Carboxypeptidase_S10.
MEROPSiS10.A12.

Proteomic databases

PaxDbiO64811.
PRIDEiO64811.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G23010.1; AT2G23010.1; AT2G23010. [O64811-1]
GeneIDi816832.
KEGGiath:AT2G23010.

Organism-specific databases

TAIRiAT2G23010.

Phylogenomic databases

eggNOGiKOG1282. Eukaryota.
COG2939. LUCA.
HOGENOMiHOG000198297.
InParanoidiO64811.
KOiK16296.
PhylomeDBiO64811.

Enzyme and pathway databases

BioCyciARA:AT2G23010-MONOMER.
ARA:GQT-492-MONOMER.
MetaCyc:AT2G23010-MONOMER.

Miscellaneous databases

PROiO64811.

Gene expression databases

ExpressionAtlasiO64811. baseline and differential.
GenevisibleiO64811. AT.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "An expression and bioinformatics analysis of the Arabidopsis serine carboxypeptidase-like gene family."
    Fraser C.M., Rider L.W., Chapple C.
    Plant Physiol. 138:1136-1148(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, TISSUE SPECIFICITY, NOMENCLATURE.
  4. "Related Arabidopsis serine carboxypeptidase-like sinapoylglucose acyltransferases display distinct but overlapping substrate specificities."
    Fraser C.M., Thompson M.G., Shirley A.M., Ralph J., Schoenherr J.A., Sinlapadech T., Hall M.C., Chapple C.
    Plant Physiol. 144:1986-1999(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiSCP9_ARATH
AccessioniPrimary (citable) accession number: O64811
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: August 1, 1998
Last modified: November 11, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was classified as a serine carboxypeptidase-like (SCPL) protein solely on the basis of the overall sequence similarity (PubMed:15908604) but it has been shown that it belongs to a class of enzymes that catalyze acyltransferase reactions (PubMed:17600138).2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.