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Protein

Putative calcium-transporting ATPase 7, plasma membrane-type

Gene

ACA7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell or into organelles.By similarity

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Enzyme regulationi

Activated by calmodulin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4554-aspartylphosphate intermediateBy similarity1
Metal bindingi756MagnesiumBy similarity1
Metal bindingi760MagnesiumBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium-transporting ATPase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • pollen development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G22950-MONOMER.
ReactomeiR-ATH-418359. Reduction of cytosolic Ca++ levels.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative calcium-transporting ATPase 7, plasma membrane-type (EC:3.6.3.8)
Alternative name(s):
Ca(2+)-ATPase isoform 7
Gene namesi
Name:ACA7
Ordered Locus Names:At2g22950
ORF Names:T20K9.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G22950.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 161CytoplasmicSequence analysisAdd BLAST161
Transmembranei162 – 182HelicalSequence analysisAdd BLAST21
Topological domaini183 – 200LumenalSequence analysisAdd BLAST18
Transmembranei201 – 221HelicalSequence analysisAdd BLAST21
Topological domaini222 – 349CytoplasmicSequence analysisAdd BLAST128
Transmembranei350 – 369HelicalSequence analysisAdd BLAST20
Topological domaini370 – 399LumenalSequence analysisAdd BLAST30
Transmembranei400 – 417HelicalSequence analysisAdd BLAST18
Topological domaini418 – 811CytoplasmicSequence analysisAdd BLAST394
Transmembranei812 – 830HelicalSequence analysisAdd BLAST19
Topological domaini831 – 841LumenalSequence analysisAdd BLAST11
Transmembranei842 – 862HelicalSequence analysisAdd BLAST21
Topological domaini863 – 882CytoplasmicSequence analysisAdd BLAST20
Transmembranei883 – 905HelicalSequence analysisAdd BLAST23
Topological domaini906 – 917LumenalSequence analysisAdd BLAST12
Transmembranei918 – 939HelicalSequence analysisAdd BLAST22
Topological domaini940 – 957CytoplasmicSequence analysisAdd BLAST18
Transmembranei958 – 979HelicalSequence analysisAdd BLAST22
Topological domaini980 – 989LumenalSequence analysis10
Transmembranei990 – 1011HelicalSequence analysisAdd BLAST22
Topological domaini1012 – 1015CytoplasmicSequence analysis4

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000464131 – 1015Putative calcium-transporting ATPase 7, plasma membrane-typeAdd BLAST1015

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei45Phosphoserine; by CPKBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO64806.
PRIDEiO64806.

PTM databases

iPTMnetiO64806.

Expressioni

Gene expression databases

GenevisibleiO64806. AT.

Interactioni

Protein-protein interaction databases

BioGridi2179. 1 interactor.
MINTiMINT-8066298.
STRINGi3702.AT2G22950.1.

Structurei

3D structure databases

ProteinModelPortaliO64806.
SMRiO64806.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 31Interaction with calmodulinBy similarityAdd BLAST12

Domaini

The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
HOGENOMiHOG000265623.
InParanoidiO64806.
KOiK01537.
OMAiCAPLTID.
OrthoDBiEOG093600UZ.
PhylomeDBiO64806.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR024750. Ca_ATPase_N_dom.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF12515. CaATP_NAI. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF08282. Hydrolase_3. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O64806-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESYLNSNFD VKAKHSSEEV LEKWRNLCSV VKNPKRRFRF TANLSKRYEA
60 70 80 90 100
AAMRRTNQEK LRIAVLVSKA AFQFISGVSP SDYKVPEEVK AAGFDICADE
110 120 130 140 150
LGSIVEGHDV KKLKFHGGVD GLSGKLKACP NAGLSTGEPE QLSKRQELFG
160 170 180 190 200
INKFAESELR SFWVFVWEAL QDMTLMILGV CAFVSLIVGI ATEGWPQGSH
210 220 230 240 250
DGLGIVASIL LVVFVTATSD YRQSLQFRDL DKEKKKITVQ VTRNGFRQKM
260 270 280 290 300
SIYDLLPGDV VHLAIGDQVP ADGLFLSGFS VVIDESSLTG ESEPVMVTAQ
310 320 330 340 350
NPFLLSGTKV QDGSCKMLVT TVGMRTQWGK LMATLSEGGD DETPLQVKLN
360 370 380 390 400
GVATIIGKIG LSFAIVTFAV LVQGMFMRKL SLGPHWWWSG DDALELLEYF
410 420 430 440 450
AIAVTIVVVA VPEGLPLAVT LSLAFAMKKM MNDKALVRHL AACETMGSAT
460 470 480 490 500
TICSDKTGTL TTNHMTVVKS CICMNVQDVA SKSSSLQSDI PEAALKLLLQ
510 520 530 540 550
LIFNNTGGEV VVNERGKTEI LGTPTETAIL ELGLSLGGKF QEERQSNKVI
560 570 580 590 600
KVEPFNSTKK RMGVVIELPE GGRIRAHTKG ASEIVLAACD KVINSSGEVV
610 620 630 640 650
PLDDESIKFL NVTIDEFANE ALRTLCLAYM DIESGFSADE GIPEKGFTCI
660 670 680 690 700
GIVGIKDPVR PGVRESVELC RRAGIMVRMV TGDNINTAKA IARECGILTD
710 720 730 740 750
DGIAIEGPVF REKNQEEMLE LIPKIQVMAR SSPMDKHTLV KQLRTTFDEV
760 770 780 790 800
VAVTGDGTND APALHEADIG LAMGIAGTEV AKEIADVIIL DDNFSTIVTV
810 820 830 840 850
AKWGRSVYIN IQKFVQFQLT VNVVALIVNF SSACLTGSAP LTAVQLLWVN
860 870 880 890 900
MIMDTLGALA LATEPPNNEL MKRMPVGRRG NFITNAMWRN ILGQAVYQFI
910 920 930 940 950
IIWILQAKGK SMFGLVGSDS TLVLNTLIFN CFVFCQVFNE VSSREMEEID
960 970 980 990 1000
VFKGILDNYV FVVVIGATVF FQIIIIEFLG TFASTTPLTI VQWFFSIFVG
1010
FLGMPIAAGL KKIPV
Length:1,015
Mass (Da):110,773
Last modified:May 1, 1999 - v2
Checksum:i57DEE2028316CFC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004401 Genomic DNA. Translation: AAF18608.2.
AC004786 Genomic DNA. Translation: AAM15005.1.
CP002685 Genomic DNA. Translation: AEC07380.1.
PIRiH84618.
RefSeqiNP_179879.1. NM_127860.2.

Genome annotation databases

EnsemblPlantsiAT2G22950.1; AT2G22950.1; AT2G22950.
GeneIDi816826.
GrameneiAT2G22950.1; AT2G22950.1; AT2G22950.
KEGGiath:AT2G22950.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004401 Genomic DNA. Translation: AAF18608.2.
AC004786 Genomic DNA. Translation: AAM15005.1.
CP002685 Genomic DNA. Translation: AEC07380.1.
PIRiH84618.
RefSeqiNP_179879.1. NM_127860.2.

3D structure databases

ProteinModelPortaliO64806.
SMRiO64806.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2179. 1 interactor.
MINTiMINT-8066298.
STRINGi3702.AT2G22950.1.

PTM databases

iPTMnetiO64806.

Proteomic databases

PaxDbiO64806.
PRIDEiO64806.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G22950.1; AT2G22950.1; AT2G22950.
GeneIDi816826.
GrameneiAT2G22950.1; AT2G22950.1; AT2G22950.
KEGGiath:AT2G22950.

Organism-specific databases

TAIRiAT2G22950.

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
HOGENOMiHOG000265623.
InParanoidiO64806.
KOiK01537.
OMAiCAPLTID.
OrthoDBiEOG093600UZ.
PhylomeDBiO64806.

Enzyme and pathway databases

BioCyciARA:AT2G22950-MONOMER.
ReactomeiR-ATH-418359. Reduction of cytosolic Ca++ levels.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiO64806.

Gene expression databases

GenevisibleiO64806. AT.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR024750. Ca_ATPase_N_dom.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF12515. CaATP_NAI. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF08282. Hydrolase_3. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACA7_ARATH
AccessioniPrimary (citable) accession number: O64806
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.