Putative calcium-transporting ATPase 7, plasma membrane-type - Arabidopsis thaliana (Mouse-ear cress)

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O64806 (ACA7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 122. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Putative calcium-transporting ATPase 7, plasma membrane-type
EC=3.6.3.8

Alternative name(s):
Ca(2+)-ATPase isoform 7

Gene names

Name:	ACA7
Ordered Locus Names:	At2g22950
ORF Names:	T20K9.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1015 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell or into organelles By similarity.
Catalytic activity	ATP + H₂O + Ca²⁺(Side 1) = ADP + phosphate + Ca²⁺(Side 2).
Enzyme regulation	Activated by calmodulin By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Domain	The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion By similarity.
Sequence similarities	Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. [View classification]

Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Calcium Calmodulin-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pollen development Inferred from mutant phenotype PubMed 22044965. Source: TAIR
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 22044965. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium-transporting ATPase activity Inferred from sequence or structural similarity PubMed 12805592. Source: TAIR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1015

1015

Putative calcium-transporting ATPase 7, plasma membrane-type

PRO_0000046413

Regions

Topological domain

1 – 161

161

Cytoplasmic Potential

Transmembrane

162 – 182

Helical; Potential

Topological domain

183 – 200

Lumenal Potential

Transmembrane

201 – 221

Helical; Potential

Topological domain

222 – 349

128

Cytoplasmic Potential

Transmembrane

350 – 369

Helical; Potential

Topological domain

370 – 399

Lumenal Potential

Transmembrane

400 – 417

Helical; Potential

Topological domain

418 – 811

394

Cytoplasmic Potential

Transmembrane

812 – 830

Helical; Potential

Topological domain

831 – 841

Lumenal Potential

Transmembrane

842 – 862

Helical; Potential

Topological domain

863 – 882

Cytoplasmic Potential

Transmembrane

883 – 905

Helical; Potential

Topological domain

906 – 917

Lumenal Potential

Transmembrane

918 – 939

Helical; Potential

Topological domain

940 – 957

Cytoplasmic Potential

Transmembrane

958 – 979

Helical; Potential

Topological domain

980 – 989

Lumenal Potential

Transmembrane

990 – 1011

Helical; Potential

Topological domain

1012 – 1015

Cytoplasmic Potential

Region

20 – 31

Interaction with calmodulin By similarity

Sites

Active site

455

4-aspartylphosphate intermediate By similarity

Metal binding

756

Magnesium By similarity

Metal binding

760

Magnesium By similarity

Amino acid modifications

Modified residue

Phosphoserine; by CPK By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

O64806 [UniParc].

Last modified May 1, 1999. Version 2.
Checksum: 57DEE2028316CFC0
Show »

FASTA

1,015

110,773

        10         20         30         40         50         60 
MESYLNSNFD VKAKHSSEEV LEKWRNLCSV VKNPKRRFRF TANLSKRYEA AAMRRTNQEK 

        70         80         90        100        110        120 
LRIAVLVSKA AFQFISGVSP SDYKVPEEVK AAGFDICADE LGSIVEGHDV KKLKFHGGVD 

       130        140        150        160        170        180 
GLSGKLKACP NAGLSTGEPE QLSKRQELFG INKFAESELR SFWVFVWEAL QDMTLMILGV 

       190        200        210        220        230        240 
CAFVSLIVGI ATEGWPQGSH DGLGIVASIL LVVFVTATSD YRQSLQFRDL DKEKKKITVQ 

       250        260        270        280        290        300 
VTRNGFRQKM SIYDLLPGDV VHLAIGDQVP ADGLFLSGFS VVIDESSLTG ESEPVMVTAQ 

       310        320        330        340        350        360 
NPFLLSGTKV QDGSCKMLVT TVGMRTQWGK LMATLSEGGD DETPLQVKLN GVATIIGKIG 

       370        380        390        400        410        420 
LSFAIVTFAV LVQGMFMRKL SLGPHWWWSG DDALELLEYF AIAVTIVVVA VPEGLPLAVT 

       430        440        450        460        470        480 
LSLAFAMKKM MNDKALVRHL AACETMGSAT TICSDKTGTL TTNHMTVVKS CICMNVQDVA 

       490        500        510        520        530        540 
SKSSSLQSDI PEAALKLLLQ LIFNNTGGEV VVNERGKTEI LGTPTETAIL ELGLSLGGKF 

       550        560        570        580        590        600 
QEERQSNKVI KVEPFNSTKK RMGVVIELPE GGRIRAHTKG ASEIVLAACD KVINSSGEVV 

       610        620        630        640        650        660 
PLDDESIKFL NVTIDEFANE ALRTLCLAYM DIESGFSADE GIPEKGFTCI GIVGIKDPVR 

       670        680        690        700        710        720 
PGVRESVELC RRAGIMVRMV TGDNINTAKA IARECGILTD DGIAIEGPVF REKNQEEMLE 

       730        740        750        760        770        780 
LIPKIQVMAR SSPMDKHTLV KQLRTTFDEV VAVTGDGTND APALHEADIG LAMGIAGTEV 

       790        800        810        820        830        840 
AKEIADVIIL DDNFSTIVTV AKWGRSVYIN IQKFVQFQLT VNVVALIVNF SSACLTGSAP 

       850        860        870        880        890        900 
LTAVQLLWVN MIMDTLGALA LATEPPNNEL MKRMPVGRRG NFITNAMWRN ILGQAVYQFI 

       910        920        930        940        950        960 
IIWILQAKGK SMFGLVGSDS TLVLNTLIFN CFVFCQVFNE VSSREMEEID VFKGILDNYV 

       970        980        990       1000       1010 
FVVVIGATVF FQIIIIEFLG TFASTTPLTI VQWFFSIFVG FLGMPIAAGL KKIPV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC004401 Genomic DNA. Translation: AAF18608.2. AC004786 Genomic DNA. Translation: AAM15005.1. CP002685 Genomic DNA. Translation: AEC07380.1.
IPI	IPI00527536.
PIR	H84618.
RefSeq	NP_179879.1. NM_127860.1.
UniGene	At.52872.
3D structure databases
ProteinModelPortal	O64806.
SMR	O64806. Positions 16-76, 117-1013.
ModBase	Search...
Proteomic databases
PaxDb	O64806.
PRIDE	O64806.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G22950.1; AT2G22950.1; AT2G22950.
GeneID	816826.
KEGG	ath:AT2G22950.
Organism-specific databases
TAIR	At2g22950.
Phylogenomic databases
eggNOG	COG0474.
HOGENOM	HOG000265623.
InParanoid	O64806.
KO	K01537.
OMA	HDGSYRM.
PhylomeDB	O64806.
ProtClustDB	CLSN2683872.
Gene expression databases
Genevestigator	O64806.
GermOnline	AT2G22950. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.20.1110.10. 2 hits. 3.40.1110.10. 1 hit.
InterPro	IPR006408. ATPase_P-typ_Ca-transp_plasma. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR023299. ATPase_P-typ_cyto_domN. IPR018303. ATPase_P-typ_P_site. IPR023298. ATPase_P-typ_TM_dom. IPR008250. ATPase_P-typ_transduc_dom_A. IPR024750. Ca_ATPase_N_dom. IPR001757. Cation_transp_P_typ_ATPase. IPR023214. HAD-like_dom. [Graphical view]
PANTHER	PTHR24093. PTHR24093. 1 hit.
Pfam	PF12515. CaATP_NAI. 1 hit. PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00120. HATPASE.
SMART	SM00831. Cation_ATPase_N. 1 hit. [Graphical view]
SUPFAM	SSF81660. ATPase_cation_domN. 1 hit. SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01517. ATPase-IIB_Ca. 1 hit. TIGR01494. ATPase_P-type. 2 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACA7_ARATH

Accession

Primary (citable) accession number: O64806

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 122 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents