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Protein

UDP-N-acetylglucosamine diphosphorylase 2

Gene

GLCNAC1PUT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Uridylyltransferase involved in the biosynthesis of UDP-glucosamine, an essential precursor for glycoprotein and glycolipid synthesis. Can use UDP-glucosamine, the 4-epimer UDP-galactosamine and UDP-glucose as substrates.1 Publication

Catalytic activityi

UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.1 Publication
UTP + N-acetyl-alpha-D-galactosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-galactosamine.1 Publication
UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.1 Publication

Cofactori

Mg2+1 Publication, Mn2+1 Publication

Kineticsi

  1. KM=180 µM for GlcNAc-1-P1 Publication
  2. KM=203 µM for UTP1 Publication
  3. KM=65 µM for UDP-GlcNAc1 Publication
  4. KM=808 µM for UDP-GalNAc1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei250 – 2501SubstrateBy similarity
    Binding sitei429 – 4291SubstrateBy similarity

    GO - Molecular functioni

    • UDP-N-acetylgalactosamine diphosphorylase activity Source: TAIR
    • UDP-N-acetylglucosamine diphosphorylase activity Source: TAIR
    • UTP:glucose-1-phosphate uridylyltransferase activity Source: TAIR

    GO - Biological processi

    • UDP-glucose metabolic process Source: TAIR
    • UDP-N-acetylgalactosamine metabolic process Source: TAIR
    • UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
    • UDP-N-acetylglucosamine metabolic process Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciARA:AT2G35020-MONOMER.
    MetaCyc:AT2G35020-MONOMER.
    BRENDAi2.7.7.23. 399.
    ReactomeiREACT_322779. Synthesis of UDP-N-acetyl-glucosamine.
    UniPathwayiUPA00113; UER00533.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-N-acetylglucosamine diphosphorylase 2 (EC:2.7.7.23)
    Alternative name(s):
    N-acetylglucosamine-1-phosphate uridylyltransferase 2
    UDP-N-acetylgalactosamine diphosphorylase 2 (EC:2.7.7.83)
    UTP--glucose-1-phosphate uridylyltransferase 2 (EC:2.7.7.9)
    Gene namesi
    Name:GLCNAC1PUT2
    Synonyms:ATUAP1
    Ordered Locus Names:At2g35020
    ORF Names:F19I3.25
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548 Componenti: Chromosome 2

    Organism-specific databases

    TAIRiAT2G35020.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 502502UDP-N-acetylglucosamine diphosphorylase 2PRO_0000185770Add
    BLAST

    Proteomic databases

    PaxDbiO64765.
    PRIDEiO64765.

    Expressioni

    Gene expression databases

    ExpressionAtlasiO64765. baseline and differential.
    GenevestigatoriO64765.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi3702.AT2G35020.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO64765.
    SMRiO64765. Positions 25-500.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi130 – 1334Substrate bindingBy similarity
    Motifi332 – 3332Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the UDPGP type 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG4284.
    HOGENOMiHOG000186273.
    InParanoidiO64765.
    KOiK00972.
    OMAiGQRWIEK.
    PhylomeDBiO64765.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR029044. Nucleotide-diphossugar_trans.
    IPR002618. UDPGP_trans_fam.
    [Graphical view]
    PANTHERiPTHR11952. PTHR11952. 1 hit.
    PfamiPF01704. UDPGP. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O64765-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKEPTTEIEI ETSAVATILP PPLPPTASPH QALVERLKDY GQEDVFSLWD
    60 70 80 90 100
    ELSPEERDLL LRDIENLDLP RIDRIIRCSL HSQGLPVAAI EPVPENCVST
    110 120 130 140 150
    VEERTKEDRE KWWKMGLKAI YEGKLGVVLL SGGQGTRLGS SDPKGCYNIG
    160 170 180 190 200
    LPSGKSLFQI QAERILCVQR LASQAMSEAS PTRPVTIQWY IMTSPFTHEP
    210 220 230 240 250
    TQKFFKSHKY FGLEPDQVTF FQQGTLPCIS KDGKFIMETP FSLSKAPDGN
    260 270 280 290 300
    GGVYTALKSS RLLEDMASRG IKYVDCYGVD NVLVRVADPT FLGYFIDKSA
    310 320 330 340 350
    ASAAKVVRKA YPQEKVGVFV RRGKGGPLTV VEYTELDQSM ASATNQQTGR
    360 370 380 390 400
    LQYCWSNVCL HMFTLDFLNQ VANGLEKDSV YHLAEKKIPS INGDIVGLKL
    410 420 430 440 450
    EQFIFDCFPY APSTALFEVL REEEFAPVKN ANGSNYDTPE SARLLVLRLH
    460 470 480 490 500
    TRWVIAAGGF LTHSVPLYAT GVEVSPLCSY AGENLEAICR GRTFHAPCEI

    SL
    Length:502
    Mass (Da):55,760
    Last modified:August 1, 1998 - v1
    Checksum:i72C0F2DCF15ACAD7
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC004238 Genomic DNA. Translation: AAC12841.1.
    CP002685 Genomic DNA. Translation: AEC09050.1.
    AF462794 mRNA. Translation: AAL58890.1.
    PIRiT00483.
    RefSeqiNP_181047.1. NM_129054.3.
    UniGeneiAt.28665.

    Genome annotation databases

    EnsemblPlantsiAT2G35020.1; AT2G35020.1; AT2G35020.
    GeneIDi818066.
    KEGGiath:AT2G35020.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC004238 Genomic DNA. Translation: AAC12841.1.
    CP002685 Genomic DNA. Translation: AEC09050.1.
    AF462794 mRNA. Translation: AAL58890.1.
    PIRiT00483.
    RefSeqiNP_181047.1. NM_129054.3.
    UniGeneiAt.28665.

    3D structure databases

    ProteinModelPortaliO64765.
    SMRiO64765. Positions 25-500.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT2G35020.1-P.

    Proteomic databases

    PaxDbiO64765.
    PRIDEiO64765.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT2G35020.1; AT2G35020.1; AT2G35020.
    GeneIDi818066.
    KEGGiath:AT2G35020.

    Organism-specific databases

    TAIRiAT2G35020.

    Phylogenomic databases

    eggNOGiCOG4284.
    HOGENOMiHOG000186273.
    InParanoidiO64765.
    KOiK00972.
    OMAiGQRWIEK.
    PhylomeDBiO64765.

    Enzyme and pathway databases

    UniPathwayiUPA00113; UER00533.
    BioCyciARA:AT2G35020-MONOMER.
    MetaCyc:AT2G35020-MONOMER.
    BRENDAi2.7.7.23. 399.
    ReactomeiREACT_322779. Synthesis of UDP-N-acetyl-glucosamine.

    Miscellaneous databases

    PROiO64765.

    Gene expression databases

    ExpressionAtlasiO64765. baseline and differential.
    GenevestigatoriO64765.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR029044. Nucleotide-diphossugar_trans.
    IPR002618. UDPGP_trans_fam.
    [Graphical view]
    PANTHERiPTHR11952. PTHR11952. 1 hit.
    PfamiPF01704. UDPGP. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Properties and physiological functions of UDP-sugar pyrophosphorylase in Arabidopsis."
      Kotake T., Hojo S., Yamaguchi D., Aohara T., Konishi T., Tsumuraya Y.
      Biosci. Biotechnol. Biochem. 71:761-771(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    5. "Identification and characterization of a strict and a promiscuous N-acetylglucosamine-1-P uridylyltransferase in Arabidopsis."
      Yang T., Echols M., Martin A., Bar-Peled M.
      Biochem. J. 430:275-284(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, 3D-STRUCTURE MODELING.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiUAP2_ARATH
    AccessioniPrimary (citable) accession number: O64765
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: August 1, 1998
    Last modified: May 27, 2015
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.