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O64765

- UAP2_ARATH

UniProt

O64765 - UAP2_ARATH

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Protein
UDP-N-acetylglucosamine diphosphorylase 2
Gene
GLCNAC1PUT2, ATUAP1, At2g35020, F19I3.25
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Uridylyltransferase involved in the biosynthesis of UDP-glucosamine, an essential precursor for glycoprotein and glycolipid synthesis. Can use UDP-glucosamine, the 4-epimer UDP-galactosamine and UDP-glucose as substrates.1 Publication

Catalytic activityi

UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.1 Publication
UTP + N-acetyl-alpha-D-galactosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-galactosamine.1 Publication
UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.1 Publication

Cofactori

Magnesium or manganese.1 Publication

Kineticsi

  1. KM=180 µM for GlcNAc-1-P1 Publication
  2. KM=203 µM for UTP
  3. KM=65 µM for UDP-GlcNAc
  4. KM=808 µM for UDP-GalNAc

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei250 – 2501Substrate By similarity
Binding sitei429 – 4291Substrate By similarity

GO - Molecular functioni

  1. UDP-N-acetylgalactosamine diphosphorylase activity Source: TAIR
  2. UDP-N-acetylglucosamine diphosphorylase activity Source: TAIR
  3. UTP:glucose-1-phosphate uridylyltransferase activity Source: TAIR

GO - Biological processi

  1. UDP-N-acetylgalactosamine metabolic process Source: TAIR
  2. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
  3. UDP-N-acetylglucosamine metabolic process Source: TAIR
  4. UDP-glucose metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT2G35020-MONOMER.
MetaCyc:AT2G35020-MONOMER.
UniPathwayiUPA00113; UER00533.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine diphosphorylase 2 (EC:2.7.7.23)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridylyltransferase 2
UDP-N-acetylgalactosamine diphosphorylase 2 (EC:2.7.7.83)
UTP--glucose-1-phosphate uridylyltransferase 2 (EC:2.7.7.9)
Gene namesi
Name:GLCNAC1PUT2
Synonyms:ATUAP1
Ordered Locus Names:At2g35020
ORF Names:F19I3.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G35020.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502UDP-N-acetylglucosamine diphosphorylase 2
PRO_0000185770Add
BLAST

Proteomic databases

PaxDbiO64765.
PRIDEiO64765.

Expressioni

Gene expression databases

GenevestigatoriO64765.

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

STRINGi3702.AT2G35020.1-P.

Structurei

3D structure databases

ProteinModelPortaliO64765.
SMRiO64765. Positions 25-500.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi130 – 1334Substrate binding By similarity
Motifi332 – 3332Substrate binding By similarity

Sequence similaritiesi

Belongs to the UDPGP type 1 family.

Phylogenomic databases

eggNOGiCOG4284.
HOGENOMiHOG000186273.
InParanoidiO64765.
KOiK00972.
OMAiVFFQQGM.
PhylomeDBiO64765.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_trans_fam.
[Graphical view]
PANTHERiPTHR11952. PTHR11952. 1 hit.
PfamiPF01704. UDPGP. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

O64765-1 [UniParc]FASTAAdd to Basket

« Hide

MKEPTTEIEI ETSAVATILP PPLPPTASPH QALVERLKDY GQEDVFSLWD    50
ELSPEERDLL LRDIENLDLP RIDRIIRCSL HSQGLPVAAI EPVPENCVST 100
VEERTKEDRE KWWKMGLKAI YEGKLGVVLL SGGQGTRLGS SDPKGCYNIG 150
LPSGKSLFQI QAERILCVQR LASQAMSEAS PTRPVTIQWY IMTSPFTHEP 200
TQKFFKSHKY FGLEPDQVTF FQQGTLPCIS KDGKFIMETP FSLSKAPDGN 250
GGVYTALKSS RLLEDMASRG IKYVDCYGVD NVLVRVADPT FLGYFIDKSA 300
ASAAKVVRKA YPQEKVGVFV RRGKGGPLTV VEYTELDQSM ASATNQQTGR 350
LQYCWSNVCL HMFTLDFLNQ VANGLEKDSV YHLAEKKIPS INGDIVGLKL 400
EQFIFDCFPY APSTALFEVL REEEFAPVKN ANGSNYDTPE SARLLVLRLH 450
TRWVIAAGGF LTHSVPLYAT GVEVSPLCSY AGENLEAICR GRTFHAPCEI 500
SL 502
Length:502
Mass (Da):55,760
Last modified:August 1, 1998 - v1
Checksum:i72C0F2DCF15ACAD7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC004238 Genomic DNA. Translation: AAC12841.1.
CP002685 Genomic DNA. Translation: AEC09050.1.
AF462794 mRNA. Translation: AAL58890.1.
PIRiT00483.
RefSeqiNP_181047.1. NM_129054.3.
UniGeneiAt.28665.

Genome annotation databases

EnsemblPlantsiAT2G35020.1; AT2G35020.1; AT2G35020.
GeneIDi818066.
KEGGiath:AT2G35020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC004238 Genomic DNA. Translation: AAC12841.1 .
CP002685 Genomic DNA. Translation: AEC09050.1 .
AF462794 mRNA. Translation: AAL58890.1 .
PIRi T00483.
RefSeqi NP_181047.1. NM_129054.3.
UniGenei At.28665.

3D structure databases

ProteinModelPortali O64765.
SMRi O64765. Positions 25-500.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G35020.1-P.

Proteomic databases

PaxDbi O64765.
PRIDEi O64765.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G35020.1 ; AT2G35020.1 ; AT2G35020 .
GeneIDi 818066.
KEGGi ath:AT2G35020.

Organism-specific databases

TAIRi AT2G35020.

Phylogenomic databases

eggNOGi COG4284.
HOGENOMi HOG000186273.
InParanoidi O64765.
KOi K00972.
OMAi VFFQQGM.
PhylomeDBi O64765.

Enzyme and pathway databases

UniPathwayi UPA00113 ; UER00533 .
BioCyci ARA:AT2G35020-MONOMER.
MetaCyc:AT2G35020-MONOMER.

Miscellaneous databases

PROi O64765.

Gene expression databases

Genevestigatori O64765.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_trans_fam.
[Graphical view ]
PANTHERi PTHR11952. PTHR11952. 1 hit.
Pfami PF01704. UDPGP. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Properties and physiological functions of UDP-sugar pyrophosphorylase in Arabidopsis."
    Kotake T., Hojo S., Yamaguchi D., Aohara T., Konishi T., Tsumuraya Y.
    Biosci. Biotechnol. Biochem. 71:761-771(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  5. "Identification and characterization of a strict and a promiscuous N-acetylglucosamine-1-P uridylyltransferase in Arabidopsis."
    Yang T., Echols M., Martin A., Bar-Peled M.
    Biochem. J. 430:275-284(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, 3D-STRUCTURE MODELING.
    Strain: cv. Columbia.

Entry informationi

Entry nameiUAP2_ARATH
AccessioniPrimary (citable) accession number: O64765
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: September 3, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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