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O64765 (UAP2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-N-acetylglucosamine diphosphorylase 2

EC=2.7.7.23
Alternative name(s):
N-acetylglucosamine-1-phosphate uridylyltransferase 2
UDP-N-acetylgalactosamine diphosphorylase 2
EC=2.7.7.83
UTP--glucose-1-phosphate uridylyltransferase 2
EC=2.7.7.9
Gene names
Name:GLCNAC1PUT2
Synonyms:ATUAP1
Ordered Locus Names:At2g35020
ORF Names:F19I3.25
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length502 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Uridylyltransferase involved in the biosynthesis of UDP-glucosamine, an essential precursor for glycoprotein and glycolipid synthesis. Can use UDP-glucosamine, the 4-epimer UDP-galactosamine and UDP-glucose as substrates. Ref.5

Catalytic activity

UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine. Ref.5

UTP + N-acetyl-alpha-D-galactosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-galactosamine. Ref.5

UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose. Ref.5

Cofactor

Magnesium or manganese. Ref.5

Pathway

Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the UDPGP type 1 family.

Biophysicochemical properties

Kinetic parameters:

KM=180 µM for GlcNAc-1-P Ref.5

KM=203 µM for UTP

KM=65 µM for UDP-GlcNAc

KM=808 µM for UDP-GalNAc

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 502502UDP-N-acetylglucosamine diphosphorylase 2
PRO_0000185770

Regions

Motif130 – 1334Substrate binding By similarity
Motif332 – 3332Substrate binding By similarity

Sites

Binding site2501Substrate By similarity
Binding site4291Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
O64765 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 72C0F2DCF15ACAD7

FASTA50255,760
        10         20         30         40         50         60 
MKEPTTEIEI ETSAVATILP PPLPPTASPH QALVERLKDY GQEDVFSLWD ELSPEERDLL 

        70         80         90        100        110        120 
LRDIENLDLP RIDRIIRCSL HSQGLPVAAI EPVPENCVST VEERTKEDRE KWWKMGLKAI 

       130        140        150        160        170        180 
YEGKLGVVLL SGGQGTRLGS SDPKGCYNIG LPSGKSLFQI QAERILCVQR LASQAMSEAS 

       190        200        210        220        230        240 
PTRPVTIQWY IMTSPFTHEP TQKFFKSHKY FGLEPDQVTF FQQGTLPCIS KDGKFIMETP 

       250        260        270        280        290        300 
FSLSKAPDGN GGVYTALKSS RLLEDMASRG IKYVDCYGVD NVLVRVADPT FLGYFIDKSA 

       310        320        330        340        350        360 
ASAAKVVRKA YPQEKVGVFV RRGKGGPLTV VEYTELDQSM ASATNQQTGR LQYCWSNVCL 

       370        380        390        400        410        420 
HMFTLDFLNQ VANGLEKDSV YHLAEKKIPS INGDIVGLKL EQFIFDCFPY APSTALFEVL 

       430        440        450        460        470        480 
REEEFAPVKN ANGSNYDTPE SARLLVLRLH TRWVIAAGGF LTHSVPLYAT GVEVSPLCSY 

       490        500 
AGENLEAICR GRTFHAPCEI SL 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Properties and physiological functions of UDP-sugar pyrophosphorylase in Arabidopsis."
Kotake T., Hojo S., Yamaguchi D., Aohara T., Konishi T., Tsumuraya Y.
Biosci. Biotechnol. Biochem. 71:761-771(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[5]"Identification and characterization of a strict and a promiscuous N-acetylglucosamine-1-P uridylyltransferase in Arabidopsis."
Yang T., Echols M., Martin A., Bar-Peled M.
Biochem. J. 430:275-284(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, 3D-STRUCTURE MODELING.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC004238 Genomic DNA. Translation: AAC12841.1.
CP002685 Genomic DNA. Translation: AEC09050.1.
AF462794 mRNA. Translation: AAL58890.1.
PIRT00483.
RefSeqNP_181047.1. NM_129054.3.
UniGeneAt.28665.

3D structure databases

ProteinModelPortalO64765.
SMRO64765. Positions 25-500.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G35020.1-P.

Proteomic databases

PaxDbO64765.
PRIDEO64765.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G35020.1; AT2G35020.1; AT2G35020.
GeneID818066.
KEGGath:AT2G35020.

Organism-specific databases

TAIRAT2G35020.

Phylogenomic databases

eggNOGCOG4284.
HOGENOMHOG000186273.
InParanoidO64765.
KOK00972.
OMAVFFQQGM.
PhylomeDBO64765.

Enzyme and pathway databases

BioCycARA:AT2G35020-MONOMER.
MetaCyc:AT2G35020-MONOMER.
UniPathwayUPA00113; UER00533.

Gene expression databases

GenevestigatorO64765.

Family and domain databases

Gene3D3.90.550.10. 1 hit.
InterProIPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_trans.
[Graphical view]
PANTHERPTHR11952. PTHR11952. 1 hit.
PfamPF01704. UDPGP. 1 hit.
[Graphical view]
SUPFAMSSF53448. SSF53448. 1 hit.
ProtoNetSearch...

Other

PROO64765.

Entry information

Entry nameUAP2_ARATH
AccessionPrimary (citable) accession number: O64765
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: June 11, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names