Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O64765

- UAP2_ARATH

UniProt

O64765 - UAP2_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

UDP-N-acetylglucosamine diphosphorylase 2

Gene

GLCNAC1PUT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Uridylyltransferase involved in the biosynthesis of UDP-glucosamine, an essential precursor for glycoprotein and glycolipid synthesis. Can use UDP-glucosamine, the 4-epimer UDP-galactosamine and UDP-glucose as substrates.1 Publication

Catalytic activityi

UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.1 Publication
UTP + N-acetyl-alpha-D-galactosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-galactosamine.1 Publication
UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.1 Publication

Cofactori

Mg2+1 Publication, Mn2+1 Publication

Kineticsi

  1. KM=180 µM for GlcNAc-1-P1 Publication
  2. KM=203 µM for UTP1 Publication
  3. KM=65 µM for UDP-GlcNAc1 Publication
  4. KM=808 µM for UDP-GalNAc1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei250 – 2501SubstrateBy similarity
Binding sitei429 – 4291SubstrateBy similarity

GO - Molecular functioni

  1. UDP-N-acetylgalactosamine diphosphorylase activity Source: TAIR
  2. UDP-N-acetylglucosamine diphosphorylase activity Source: TAIR
  3. UTP:glucose-1-phosphate uridylyltransferase activity Source: TAIR

GO - Biological processi

  1. UDP-glucose metabolic process Source: TAIR
  2. UDP-N-acetylgalactosamine metabolic process Source: TAIR
  3. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
  4. UDP-N-acetylglucosamine metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT2G35020-MONOMER.
MetaCyc:AT2G35020-MONOMER.
ReactomeiREACT_190863. Synthesis of UDP-N-acetyl-glucosamine.
UniPathwayiUPA00113; UER00533.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine diphosphorylase 2 (EC:2.7.7.23)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridylyltransferase 2
UDP-N-acetylgalactosamine diphosphorylase 2 (EC:2.7.7.83)
UTP--glucose-1-phosphate uridylyltransferase 2 (EC:2.7.7.9)
Gene namesi
Name:GLCNAC1PUT2
Synonyms:ATUAP1
Ordered Locus Names:At2g35020
ORF Names:F19I3.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G35020.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502UDP-N-acetylglucosamine diphosphorylase 2PRO_0000185770Add
BLAST

Proteomic databases

PaxDbiO64765.
PRIDEiO64765.

Expressioni

Gene expression databases

ExpressionAtlasiO64765. baseline and differential.
GenevestigatoriO64765.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi3702.AT2G35020.1-P.

Structurei

3D structure databases

ProteinModelPortaliO64765.
SMRiO64765. Positions 25-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi130 – 1334Substrate bindingBy similarity
Motifi332 – 3332Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the UDPGP type 1 family.Curated

Phylogenomic databases

eggNOGiCOG4284.
HOGENOMiHOG000186273.
InParanoidiO64765.
KOiK00972.
OMAiVFFQQGM.
PhylomeDBiO64765.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_trans_fam.
[Graphical view]
PANTHERiPTHR11952. PTHR11952. 1 hit.
PfamiPF01704. UDPGP. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

O64765-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKEPTTEIEI ETSAVATILP PPLPPTASPH QALVERLKDY GQEDVFSLWD
60 70 80 90 100
ELSPEERDLL LRDIENLDLP RIDRIIRCSL HSQGLPVAAI EPVPENCVST
110 120 130 140 150
VEERTKEDRE KWWKMGLKAI YEGKLGVVLL SGGQGTRLGS SDPKGCYNIG
160 170 180 190 200
LPSGKSLFQI QAERILCVQR LASQAMSEAS PTRPVTIQWY IMTSPFTHEP
210 220 230 240 250
TQKFFKSHKY FGLEPDQVTF FQQGTLPCIS KDGKFIMETP FSLSKAPDGN
260 270 280 290 300
GGVYTALKSS RLLEDMASRG IKYVDCYGVD NVLVRVADPT FLGYFIDKSA
310 320 330 340 350
ASAAKVVRKA YPQEKVGVFV RRGKGGPLTV VEYTELDQSM ASATNQQTGR
360 370 380 390 400
LQYCWSNVCL HMFTLDFLNQ VANGLEKDSV YHLAEKKIPS INGDIVGLKL
410 420 430 440 450
EQFIFDCFPY APSTALFEVL REEEFAPVKN ANGSNYDTPE SARLLVLRLH
460 470 480 490 500
TRWVIAAGGF LTHSVPLYAT GVEVSPLCSY AGENLEAICR GRTFHAPCEI

SL
Length:502
Mass (Da):55,760
Last modified:August 1, 1998 - v1
Checksum:i72C0F2DCF15ACAD7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004238 Genomic DNA. Translation: AAC12841.1.
CP002685 Genomic DNA. Translation: AEC09050.1.
AF462794 mRNA. Translation: AAL58890.1.
PIRiT00483.
RefSeqiNP_181047.1. NM_129054.3.
UniGeneiAt.28665.

Genome annotation databases

EnsemblPlantsiAT2G35020.1; AT2G35020.1; AT2G35020.
GeneIDi818066.
KEGGiath:AT2G35020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004238 Genomic DNA. Translation: AAC12841.1 .
CP002685 Genomic DNA. Translation: AEC09050.1 .
AF462794 mRNA. Translation: AAL58890.1 .
PIRi T00483.
RefSeqi NP_181047.1. NM_129054.3.
UniGenei At.28665.

3D structure databases

ProteinModelPortali O64765.
SMRi O64765. Positions 25-500.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G35020.1-P.

Proteomic databases

PaxDbi O64765.
PRIDEi O64765.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G35020.1 ; AT2G35020.1 ; AT2G35020 .
GeneIDi 818066.
KEGGi ath:AT2G35020.

Organism-specific databases

TAIRi AT2G35020.

Phylogenomic databases

eggNOGi COG4284.
HOGENOMi HOG000186273.
InParanoidi O64765.
KOi K00972.
OMAi VFFQQGM.
PhylomeDBi O64765.

Enzyme and pathway databases

UniPathwayi UPA00113 ; UER00533 .
BioCyci ARA:AT2G35020-MONOMER.
MetaCyc:AT2G35020-MONOMER.
Reactomei REACT_190863. Synthesis of UDP-N-acetyl-glucosamine.

Miscellaneous databases

PROi O64765.

Gene expression databases

ExpressionAtlasi O64765. baseline and differential.
Genevestigatori O64765.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_trans_fam.
[Graphical view ]
PANTHERi PTHR11952. PTHR11952. 1 hit.
Pfami PF01704. UDPGP. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Properties and physiological functions of UDP-sugar pyrophosphorylase in Arabidopsis."
    Kotake T., Hojo S., Yamaguchi D., Aohara T., Konishi T., Tsumuraya Y.
    Biosci. Biotechnol. Biochem. 71:761-771(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  5. "Identification and characterization of a strict and a promiscuous N-acetylglucosamine-1-P uridylyltransferase in Arabidopsis."
    Yang T., Echols M., Martin A., Bar-Peled M.
    Biochem. J. 430:275-284(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, 3D-STRUCTURE MODELING.
    Strain: cv. Columbia.

Entry informationi

Entry nameiUAP2_ARATH
AccessioniPrimary (citable) accession number: O64765
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: November 26, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3