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O64765

- UAP2_ARATH

UniProt

O64765 - UAP2_ARATH

Protein

UDP-N-acetylglucosamine diphosphorylase 2

Gene

GLCNAC1PUT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Uridylyltransferase involved in the biosynthesis of UDP-glucosamine, an essential precursor for glycoprotein and glycolipid synthesis. Can use UDP-glucosamine, the 4-epimer UDP-galactosamine and UDP-glucose as substrates.1 Publication

    Catalytic activityi

    UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.1 Publication
    UTP + N-acetyl-alpha-D-galactosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-galactosamine.1 Publication
    UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.1 Publication

    Cofactori

    Magnesium or manganese.1 Publication

    Kineticsi

    1. KM=180 µM for GlcNAc-1-P1 Publication
    2. KM=203 µM for UTP1 Publication
    3. KM=65 µM for UDP-GlcNAc1 Publication
    4. KM=808 µM for UDP-GalNAc1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei250 – 2501SubstrateBy similarity
    Binding sitei429 – 4291SubstrateBy similarity

    GO - Molecular functioni

    1. UDP-N-acetylgalactosamine diphosphorylase activity Source: TAIR
    2. UDP-N-acetylglucosamine diphosphorylase activity Source: TAIR
    3. UTP:glucose-1-phosphate uridylyltransferase activity Source: TAIR

    GO - Biological processi

    1. UDP-glucose metabolic process Source: TAIR
    2. UDP-N-acetylgalactosamine metabolic process Source: TAIR
    3. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
    4. UDP-N-acetylglucosamine metabolic process Source: TAIR

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciARA:AT2G35020-MONOMER.
    MetaCyc:AT2G35020-MONOMER.
    UniPathwayiUPA00113; UER00533.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-N-acetylglucosamine diphosphorylase 2 (EC:2.7.7.23)
    Alternative name(s):
    N-acetylglucosamine-1-phosphate uridylyltransferase 2
    UDP-N-acetylgalactosamine diphosphorylase 2 (EC:2.7.7.83)
    UTP--glucose-1-phosphate uridylyltransferase 2 (EC:2.7.7.9)
    Gene namesi
    Name:GLCNAC1PUT2
    Synonyms:ATUAP1
    Ordered Locus Names:At2g35020
    ORF Names:F19I3.25
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G35020.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 502502UDP-N-acetylglucosamine diphosphorylase 2PRO_0000185770Add
    BLAST

    Proteomic databases

    PaxDbiO64765.
    PRIDEiO64765.

    Expressioni

    Gene expression databases

    GenevestigatoriO64765.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi3702.AT2G35020.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO64765.
    SMRiO64765. Positions 25-500.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi130 – 1334Substrate bindingBy similarity
    Motifi332 – 3332Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the UDPGP type 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG4284.
    HOGENOMiHOG000186273.
    InParanoidiO64765.
    KOiK00972.
    OMAiVFFQQGM.
    PhylomeDBiO64765.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR029044. Nucleotide-diphossugar_trans.
    IPR002618. UDPGP_trans_fam.
    [Graphical view]
    PANTHERiPTHR11952. PTHR11952. 1 hit.
    PfamiPF01704. UDPGP. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O64765-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKEPTTEIEI ETSAVATILP PPLPPTASPH QALVERLKDY GQEDVFSLWD    50
    ELSPEERDLL LRDIENLDLP RIDRIIRCSL HSQGLPVAAI EPVPENCVST 100
    VEERTKEDRE KWWKMGLKAI YEGKLGVVLL SGGQGTRLGS SDPKGCYNIG 150
    LPSGKSLFQI QAERILCVQR LASQAMSEAS PTRPVTIQWY IMTSPFTHEP 200
    TQKFFKSHKY FGLEPDQVTF FQQGTLPCIS KDGKFIMETP FSLSKAPDGN 250
    GGVYTALKSS RLLEDMASRG IKYVDCYGVD NVLVRVADPT FLGYFIDKSA 300
    ASAAKVVRKA YPQEKVGVFV RRGKGGPLTV VEYTELDQSM ASATNQQTGR 350
    LQYCWSNVCL HMFTLDFLNQ VANGLEKDSV YHLAEKKIPS INGDIVGLKL 400
    EQFIFDCFPY APSTALFEVL REEEFAPVKN ANGSNYDTPE SARLLVLRLH 450
    TRWVIAAGGF LTHSVPLYAT GVEVSPLCSY AGENLEAICR GRTFHAPCEI 500
    SL 502
    Length:502
    Mass (Da):55,760
    Last modified:August 1, 1998 - v1
    Checksum:i72C0F2DCF15ACAD7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004238 Genomic DNA. Translation: AAC12841.1.
    CP002685 Genomic DNA. Translation: AEC09050.1.
    AF462794 mRNA. Translation: AAL58890.1.
    PIRiT00483.
    RefSeqiNP_181047.1. NM_129054.3.
    UniGeneiAt.28665.

    Genome annotation databases

    EnsemblPlantsiAT2G35020.1; AT2G35020.1; AT2G35020.
    GeneIDi818066.
    KEGGiath:AT2G35020.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004238 Genomic DNA. Translation: AAC12841.1 .
    CP002685 Genomic DNA. Translation: AEC09050.1 .
    AF462794 mRNA. Translation: AAL58890.1 .
    PIRi T00483.
    RefSeqi NP_181047.1. NM_129054.3.
    UniGenei At.28665.

    3D structure databases

    ProteinModelPortali O64765.
    SMRi O64765. Positions 25-500.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G35020.1-P.

    Proteomic databases

    PaxDbi O64765.
    PRIDEi O64765.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G35020.1 ; AT2G35020.1 ; AT2G35020 .
    GeneIDi 818066.
    KEGGi ath:AT2G35020.

    Organism-specific databases

    TAIRi AT2G35020.

    Phylogenomic databases

    eggNOGi COG4284.
    HOGENOMi HOG000186273.
    InParanoidi O64765.
    KOi K00972.
    OMAi VFFQQGM.
    PhylomeDBi O64765.

    Enzyme and pathway databases

    UniPathwayi UPA00113 ; UER00533 .
    BioCyci ARA:AT2G35020-MONOMER.
    MetaCyc:AT2G35020-MONOMER.

    Miscellaneous databases

    PROi O64765.

    Gene expression databases

    Genevestigatori O64765.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR029044. Nucleotide-diphossugar_trans.
    IPR002618. UDPGP_trans_fam.
    [Graphical view ]
    PANTHERi PTHR11952. PTHR11952. 1 hit.
    Pfami PF01704. UDPGP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Properties and physiological functions of UDP-sugar pyrophosphorylase in Arabidopsis."
      Kotake T., Hojo S., Yamaguchi D., Aohara T., Konishi T., Tsumuraya Y.
      Biosci. Biotechnol. Biochem. 71:761-771(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    5. "Identification and characterization of a strict and a promiscuous N-acetylglucosamine-1-P uridylyltransferase in Arabidopsis."
      Yang T., Echols M., Martin A., Bar-Peled M.
      Biochem. J. 430:275-284(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, 3D-STRUCTURE MODELING.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiUAP2_ARATH
    AccessioniPrimary (citable) accession number: O64765
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3