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O64730

- P2C26_ARATH

UniProt

O64730 - P2C26_ARATH

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Protein

Probable protein phosphatase 2C 26

Gene

At2g30170

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi82 – 821Manganese 1By similarity
Metal bindingi82 – 821Manganese 2By similarity
Metal bindingi83 – 831Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi213 – 2131Manganese 2By similarity
Metal bindingi286 – 2861Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW

GO - Biological processi

  1. cellular response to light stimulus Source: TAIR
  2. peptidyl-threonine dephosphorylation Source: TAIR
  3. thylakoid membrane organization Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G30170-MONOMER.
ARA:GQT-463-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 26 (EC:3.1.3.16)
Short name:
AtPP2C26
Gene namesi
Ordered Locus Names:At2g30170
ORF Names:T27E13.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G30170.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Probable protein phosphatase 2C 26PRO_0000367955Add
BLAST

Proteomic databases

PaxDbiO64730.
PRIDEiO64730.

Expressioni

Gene expression databases

GenevestigatoriO64730.

Interactioni

Protein-protein interaction databases

BioGridi2918. 1 interaction.
IntActiO64730. 1 interaction.
MINTiMINT-8390765.
STRINGi3702.AT2G30170.1-P.

Structurei

3D structure databases

ProteinModelPortaliO64730.
SMRiO64730. Positions 45-297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 295250PP2C-likeAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PP2C-like domain.Curated

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000237527.
InParanoidiO64730.
OMAiDNVFDHE.
PhylomeDBiO64730.

Family and domain databases

Gene3Di3.60.40.10. 2 hits.
InterProiIPR001932. PP2C-like_dom.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O64730-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAIPVTRMMV PHAIPSLRLS HPNPSRVDFL CRCAPSEIQP LRPELSLSVG
60 70 80 90 100
IHAIPHPDKV EKGGEDAFFV SSYRGGVMAV ADGVSGWAEQ DVDPSLFSKE
110 120 130 140 150
LMANASRLVD DQEVRYDPGF LIDKAHTATT SRGSATIILA MLEEVGILKI
160 170 180 190 200
GNVGDCGLKL LREGQIIFAT APQEHYFDCP YQLSSEGSAQ TYLDASFSIV
210 220 230 240 250
EVQKGDVIVM GSDGLFDNVF DHEIVSIVTK HTDVAESSRL LAEVASSHSR
260 270 280 290
DTEFESPYAL EARAKGFDVP LWKKVLGKKL TGGKLDDVTV IVAKVVSS
Length:298
Mass (Da):32,285
Last modified:June 1, 2002 - v2
Checksum:i52327F83126D89F5
GO
Isoform 2 (identifier: O64730-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-77: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:221
Mass (Da):23,886
Checksum:i65E5747759CB349B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7777Missing in isoform 2. CuratedVSP_036765Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC004165 Genomic DNA. Translation: AAC16955.2.
CP002685 Genomic DNA. Translation: AEC08354.1.
CP002685 Genomic DNA. Translation: AEC08355.1.
AF370298 mRNA. Translation: AAK44113.1.
AY063046 mRNA. Translation: AAL34220.1.
PIRiT00581.
RefSeqiNP_001077980.1. NM_001084511.2. [O64730-2]
NP_565696.1. NM_128572.4. [O64730-1]
UniGeneiAt.19566.

Genome annotation databases

EnsemblPlantsiAT2G30170.1; AT2G30170.1; AT2G30170. [O64730-1]
GeneIDi817569.
KEGGiath:AT2G30170.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC004165 Genomic DNA. Translation: AAC16955.2 .
CP002685 Genomic DNA. Translation: AEC08354.1 .
CP002685 Genomic DNA. Translation: AEC08355.1 .
AF370298 mRNA. Translation: AAK44113.1 .
AY063046 mRNA. Translation: AAL34220.1 .
PIRi T00581.
RefSeqi NP_001077980.1. NM_001084511.2. [O64730-2 ]
NP_565696.1. NM_128572.4. [O64730-1 ]
UniGenei At.19566.

3D structure databases

ProteinModelPortali O64730.
SMRi O64730. Positions 45-297.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 2918. 1 interaction.
IntActi O64730. 1 interaction.
MINTi MINT-8390765.
STRINGi 3702.AT2G30170.1-P.

Proteomic databases

PaxDbi O64730.
PRIDEi O64730.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G30170.1 ; AT2G30170.1 ; AT2G30170 . [O64730-1 ]
GeneIDi 817569.
KEGGi ath:AT2G30170.

Organism-specific databases

TAIRi AT2G30170.

Phylogenomic databases

eggNOGi COG0631.
HOGENOMi HOG000237527.
InParanoidi O64730.
OMAi DNVFDHE.
PhylomeDBi O64730.

Enzyme and pathway databases

BioCyci ARA:AT2G30170-MONOMER.
ARA:GQT-463-MONOMER.

Gene expression databases

Genevestigatori O64730.

Family and domain databases

Gene3Di 3.60.40.10. 2 hits.
InterProi IPR001932. PP2C-like_dom.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiP2C26_ARATH
AccessioniPrimary (citable) accession number: O64730
Secondary accession number(s): A8MQM8, Q94K51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3