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O64730

- P2C26_ARATH

UniProt

O64730 - P2C26_ARATH

Protein

Probable protein phosphatase 2C 26

Gene

At2g30170

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 2 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi82 – 821Manganese 1By similarity
    Metal bindingi82 – 821Manganese 2By similarity
    Metal bindingi83 – 831Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi213 – 2131Manganese 2By similarity
    Metal bindingi286 – 2861Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phosphoprotein phosphatase activity Source: UniProtKB-KW

    GO - Biological processi

    1. cellular response to light stimulus Source: TAIR
    2. peptidyl-threonine dephosphorylation Source: TAIR
    3. thylakoid membrane organization Source: TAIR

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G30170-MONOMER.
    ARA:GQT-463-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable protein phosphatase 2C 26 (EC:3.1.3.16)
    Short name:
    AtPP2C26
    Gene namesi
    Ordered Locus Names:At2g30170
    ORF Names:T27E13.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G30170.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 298298Probable protein phosphatase 2C 26PRO_0000367955Add
    BLAST

    Proteomic databases

    PaxDbiO64730.
    PRIDEiO64730.

    Expressioni

    Gene expression databases

    GenevestigatoriO64730.

    Interactioni

    Protein-protein interaction databases

    BioGridi2918. 1 interaction.
    IntActiO64730. 1 interaction.
    MINTiMINT-8390765.
    STRINGi3702.AT2G30170.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO64730.
    SMRiO64730. Positions 45-297.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini46 – 295250PP2C-likeAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PP2C family.Curated
    Contains 1 PP2C-like domain.Curated

    Phylogenomic databases

    eggNOGiCOG0631.
    HOGENOMiHOG000237527.
    InParanoidiQ94K51.
    OMAiDNVFDHE.
    PhylomeDBiO64730.

    Family and domain databases

    Gene3Di3.60.40.10. 2 hits.
    InterProiIPR001932. PP2C-like_dom.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 2 hits.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O64730-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAIPVTRMMV PHAIPSLRLS HPNPSRVDFL CRCAPSEIQP LRPELSLSVG    50
    IHAIPHPDKV EKGGEDAFFV SSYRGGVMAV ADGVSGWAEQ DVDPSLFSKE 100
    LMANASRLVD DQEVRYDPGF LIDKAHTATT SRGSATIILA MLEEVGILKI 150
    GNVGDCGLKL LREGQIIFAT APQEHYFDCP YQLSSEGSAQ TYLDASFSIV 200
    EVQKGDVIVM GSDGLFDNVF DHEIVSIVTK HTDVAESSRL LAEVASSHSR 250
    DTEFESPYAL EARAKGFDVP LWKKVLGKKL TGGKLDDVTV IVAKVVSS 298
    Length:298
    Mass (Da):32,285
    Last modified:June 1, 2002 - v2
    Checksum:i52327F83126D89F5
    GO
    Isoform 2 (identifier: O64730-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-77: Missing.

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:221
    Mass (Da):23,886
    Checksum:i65E5747759CB349B
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 7777Missing in isoform 2. CuratedVSP_036765Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004165 Genomic DNA. Translation: AAC16955.2.
    CP002685 Genomic DNA. Translation: AEC08354.1.
    CP002685 Genomic DNA. Translation: AEC08355.1.
    AF370298 mRNA. Translation: AAK44113.1.
    AY063046 mRNA. Translation: AAL34220.1.
    PIRiT00581.
    RefSeqiNP_001077980.1. NM_001084511.2. [O64730-2]
    NP_565696.1. NM_128572.4. [O64730-1]
    UniGeneiAt.19566.

    Genome annotation databases

    EnsemblPlantsiAT2G30170.1; AT2G30170.1; AT2G30170. [O64730-1]
    GeneIDi817569.
    KEGGiath:AT2G30170.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004165 Genomic DNA. Translation: AAC16955.2 .
    CP002685 Genomic DNA. Translation: AEC08354.1 .
    CP002685 Genomic DNA. Translation: AEC08355.1 .
    AF370298 mRNA. Translation: AAK44113.1 .
    AY063046 mRNA. Translation: AAL34220.1 .
    PIRi T00581.
    RefSeqi NP_001077980.1. NM_001084511.2. [O64730-2 ]
    NP_565696.1. NM_128572.4. [O64730-1 ]
    UniGenei At.19566.

    3D structure databases

    ProteinModelPortali O64730.
    SMRi O64730. Positions 45-297.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 2918. 1 interaction.
    IntActi O64730. 1 interaction.
    MINTi MINT-8390765.
    STRINGi 3702.AT2G30170.1-P.

    Proteomic databases

    PaxDbi O64730.
    PRIDEi O64730.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G30170.1 ; AT2G30170.1 ; AT2G30170 . [O64730-1 ]
    GeneIDi 817569.
    KEGGi ath:AT2G30170.

    Organism-specific databases

    TAIRi AT2G30170.

    Phylogenomic databases

    eggNOGi COG0631.
    HOGENOMi HOG000237527.
    InParanoidi Q94K51.
    OMAi DNVFDHE.
    PhylomeDBi O64730.

    Enzyme and pathway databases

    BioCyci ARA:AT2G30170-MONOMER.
    ARA:GQT-463-MONOMER.

    Gene expression databases

    Genevestigatori O64730.

    Family and domain databases

    Gene3Di 3.60.40.10. 2 hits.
    InterProi IPR001932. PP2C-like_dom.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    4. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
      Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
      BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiP2C26_ARATH
    AccessioniPrimary (citable) accession number: O64730
    Secondary accession number(s): A8MQM8, Q94K51
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 88 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3