O64730 (P2C26_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 26
Short name=AtPP2C26
EC=3.1.3.16

Gene names

Ordered Locus Names:	At2g30170
ORF Names:	T27E13.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to light stimulus Inferred from mutant phenotype PubMed 22706287. Source: TAIR peptidyl-threonine dephosphorylation Inferred from direct assay PubMed 22706287. Source: TAIR thylakoid membrane organization Inferred from mutant phenotype PubMed 22706287. Source: TAIR
Cellular_component	chloroplast stroma Inferred from direct assay PubMed 20061580. Source: TAIR
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O64730-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O64730-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-77: Missing.

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	Probable protein phosphatase 2C 26		PRO_0000367955
Regions
	Domain	46 – 295	250	PP2C-like
Sites
	Metal binding	82	1	Manganese 1 By similarity
	Metal binding	82	1	Manganese 2 By similarity
	Metal binding	83	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	213	1	Manganese 2 By similarity
	Metal binding	286	1	Manganese 2 By similarity
Natural variations
	Alternative sequence	1 – 77	77	Missing in isoform 2.		VSP_036765

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2002. Version 2. Checksum: 52327F83126D89F5	FASTA	298	32,285
	10 20 30 40 50 60 MAIPVTRMMV PHAIPSLRLS HPNPSRVDFL CRCAPSEIQP LRPELSLSVG IHAIPHPDKV 70 80 90 100 110 120 EKGGEDAFFV SSYRGGVMAV ADGVSGWAEQ DVDPSLFSKE LMANASRLVD DQEVRYDPGF 130 140 150 160 170 180 LIDKAHTATT SRGSATIILA MLEEVGILKI GNVGDCGLKL LREGQIIFAT APQEHYFDCP 190 200 210 220 230 240 YQLSSEGSAQ TYLDASFSIV EVQKGDVIVM GSDGLFDNVF DHEIVSIVTK HTDVAESSRL 250 260 270 280 290 LAEVASSHSR DTEFESPYAL EARAKGFDVP LWKKVLGKKL TGGKLDDVTV IVAKVVSS
	Isoform 2 [UniParc]. Checksum: 65E5747759CB349B	FASTA	221	23,886
	10 20 30 40 50 60 MAVADGVSGW AEQDVDPSLF SKELMANASR LVDDQEVRYD PGFLIDKAHT ATTSRGSATI 70 80 90 100 110 120 ILAMLEEVGI LKIGNVGDCG LKLLREGQII FATAPQEHYF DCPYQLSSEG SAQTYLDASF 130 140 150 160 170 180 SIVEVQKGDV IVMGSDGLFD NVFDHEIVSI VTKHTDVAES SRLLAEVASS HSRDTEFESP 190 200 210 220 YALEARAKGF DVPLWKKVLG KKLTGGKLDD VTVIVAKVVS S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC004165 Genomic DNA. Translation: AAC16955.2. CP002685 Genomic DNA. Translation: AEC08354.1. CP002685 Genomic DNA. Translation: AEC08355.1. AF370298 mRNA. Translation: AAK44113.1. AY063046 mRNA. Translation: AAL34220.1.
IPI	IPI00529715. IPI00846129.
PIR	T00581.
RefSeq	NP_001077980.1. NM_001084511.2. NP_565696.1. NM_128572.4.
UniGene	At.19566.
3D structure databases
ProteinModelPortal	O64730.
SMR	O64730. Positions 51-296.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-8390765.
STRING	3702.AT2G30170.1-P.
Proteomic databases
PaxDb	O64730.
PRIDE	O64730.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G30170.1; AT2G30170.1; AT2G30170.
GeneID	817569.
KEGG	ath:AT2G30170.
Organism-specific databases
TAIR	At2g30170.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000237527.
InParanoid	Q94K51.
OMA	QEHYFDC.
PhylomeDB	O64730.
ProtClustDB	CLSN2688568.
Gene expression databases
Genevestigator	O64730.
Family and domain databases
Gene3D	3.60.40.10. 2 hits.
InterPro	IPR001932. PP2C-like. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C26_ARATH

Accession

Primary (citable) accession number: O64730
Secondary accession number(s): A8MQM8, Q94K51

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 1, 2002
Last modified:	May 29, 2013
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program