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Protein

Cytochrome P450 710A2

Gene

CYP710A2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required to form the C-22 double bond in the sterol side chain. Possesses in vitro C-22 desaturase activity toward 24-epi-campesterol and beta-sitosterol and produces brassicasterol and stigmasterol, respectively. No activity with campesterol.1 Publication

Miscellaneous

Plants overexpressing CYP710A2 show higher levels of brassicasterol/crinosterol and stigmasterol, and lower levels of 24-epi-campesterol/campesterol and beta-sitosterol than the wild-type.1 Publication

Catalytic activityi

Ergosta-5,7,24(28)-trien-3-beta-ol + NADPH + O2 = ergosta-5,7,22,24(28)-tetraen-3-beta-ol + NADP+ + 2 H2O.1 Publication

Cofactori

hemeBy similarity

Pathwayi: sterol biosynthesis

This protein is involved in the pathway sterol biosynthesis, which is part of Steroid biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway sterol biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi439Iron (heme axial ligand)By similarity1

GO - Molecular functioni

  • C-22 sterol desaturase activity Source: TAIR
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro
  • monooxygenase activity Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT2G34490-MONOMER
MetaCyc:AT2G34490-MONOMER
UniPathwayiUPA00766

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 710A21 Publication (EC:1.14.19.411 Publication)
Alternative name(s):
C-22 sterol desaturase1 Publication
Gene namesi
Name:CYP710A21 Publication
Ordered Locus Names:At2g34490Imported
ORF Names:F13P17.22Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G34490
TAIRilocus:2040929 AT2G34490

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei5 – 25HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but production of brassicasterol is abolished.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004112061 – 499Cytochrome P450 710A2Add BLAST499

Proteomic databases

PaxDbiO64698

Expressioni

Tissue specificityi

Expressed in the vascular tissues of roots, shoots, stems and leaves. Expressed in root tips, carpes, siliques and seeds.1 Publication

Inductioni

Not induced by pathogen infection.1 Publication

Gene expression databases

ExpressionAtlasiO64698 baseline and differential
GenevisibleiO64698 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G34490.1

Structurei

3D structure databases

ProteinModelPortaliO64698
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0157 Eukaryota
COG2124 LUCA
HOGENOMiHOG000237640
InParanoidiO64698
KOiK09832
OMAiIVIASNC
OrthoDBiEOG093607IL
PhylomeDBiO64698

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

O64698-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVFSVSIFAS LAPYLVSALL LFFLIEQLSY LVKKRNLPGP LFVPPIIGNA
60 70 80 90 100
ISLVRDPTSF WFKQSDTAGT SPGLAANYLI GKFIIYIRDT ELSHQIFSNV
110 120 130 140 150
RLEAFHPLGH PFGKQLFGDH SLIYLFGEDH KTVRRHLAPN FTPKALSTYS
160 170 180 190 200
DLQQIVMLRH LRQWEESFSG GTKPVSMRDL VRELNLETSQ TVFVGPYLDK
210 220 230 240 250
EARNTFCTDY NLFNLGSMAL PINLPGFAFN KARRAVMNLE KTLSVCAGKS
260 270 280 290 300
KKRMATGEEP TCLIDFWMHA FVTEIESGNP PPLHSEDEAI GGLLFDFLFA
310 320 330 340 350
AQDASTSSLL WAVTFLESHP KVLSKVREEV AKIWSPQSGH LITADQLAEM
360 370 380 390 400
KYTRAVAREV VRYRPPATMV PHIATNDFPL TESYTIPKGT IVFPSVFDAS
410 420 430 440 450
FQGFTEPNRF DPDRFSETRQ EDQVFKRNYL AFGWGAHQCV GQRYALNHLV
460 470 480 490
LFIAMFSSLF DFKRLQSDGC DDIIYCPTIS PKDGCTVFLS KRIVTYPNL
Length:499
Mass (Da):56,346
Last modified:August 1, 1998 - v1
Checksum:i6CC7D1CBC0474348
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18A → S in AAM62799 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB233425 Genomic DNA Translation: BAE80316.1
AC004077 Genomic DNA Translation: AAC26691.1
AC004481 Genomic DNA Translation: AAM14945.1
CP002685 Genomic DNA Translation: AEC08981.1
AY074867 mRNA Translation: AAL75888.1
AY101543 mRNA Translation: AAM26664.1
AY085577 mRNA Translation: AAM62799.1
PIRiT02336
RefSeqiNP_180996.1, NM_129001.3
UniGeneiAt.27927

Genome annotation databases

EnsemblPlantsiAT2G34490.1; AT2G34490.1; AT2G34490
GeneIDi818012
GrameneiAT2G34490.1; AT2G34490.1; AT2G34490
KEGGiath:AT2G34490

Similar proteinsi

Entry informationi

Entry nameiC7102_ARATH
AccessioniPrimary (citable) accession number: O64698
Secondary accession number(s): Q8LE78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health