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O64698

- C7102_ARATH

UniProt

O64698 - C7102_ARATH

Protein

Cytochrome P450 710A2

Gene

CYP710A2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Required to form the C-22 double bond in the sterol side chain. Possesses in vitro C-22 desaturase activity toward 24-epi-campesterol and beta-sitosterol and produces brassicasterol and stigmasterol, respectively.1 Publication

    Cofactori

    Heme group.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi439 – 4391Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. C-22 sterol desaturase activity Source: TAIR
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. monooxygenase activity Source: UniProtKB-KW

    GO - Biological processi

    1. sterol biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G34490-MONOMER.
    MetaCyc:AT2G34490-MONOMER.
    UniPathwayiUPA00766.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 710A2 (EC:1.14.-.-)
    Alternative name(s):
    C-22 sterol desaturase
    Gene namesi
    Name:CYP710A2
    Ordered Locus Names:At2g34490
    ORF Names:F13P17.22
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G34490.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth conditions, but production of brassicasterol is abolished.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 499499Cytochrome P450 710A2PRO_0000411206Add
    BLAST

    Proteomic databases

    PaxDbiO64698.
    PRIDEiO64698.

    Expressioni

    Tissue specificityi

    Expressed in the vascular tissues of roots, shoots, stems and leaves. Expressed in root tips, carpes, siliques and seeds.1 Publication

    Gene expression databases

    GenevestigatoriO64698.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G34490.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO64698.
    SMRiO64698. Positions 31-493.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei5 – 2521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000237640.
    InParanoidiO64698.
    KOiK09832.
    OMAiAFPITPD.
    PhylomeDBiO64698.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O64698-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVFSVSIFAS LAPYLVSALL LFFLIEQLSY LVKKRNLPGP LFVPPIIGNA    50
    ISLVRDPTSF WFKQSDTAGT SPGLAANYLI GKFIIYIRDT ELSHQIFSNV 100
    RLEAFHPLGH PFGKQLFGDH SLIYLFGEDH KTVRRHLAPN FTPKALSTYS 150
    DLQQIVMLRH LRQWEESFSG GTKPVSMRDL VRELNLETSQ TVFVGPYLDK 200
    EARNTFCTDY NLFNLGSMAL PINLPGFAFN KARRAVMNLE KTLSVCAGKS 250
    KKRMATGEEP TCLIDFWMHA FVTEIESGNP PPLHSEDEAI GGLLFDFLFA 300
    AQDASTSSLL WAVTFLESHP KVLSKVREEV AKIWSPQSGH LITADQLAEM 350
    KYTRAVAREV VRYRPPATMV PHIATNDFPL TESYTIPKGT IVFPSVFDAS 400
    FQGFTEPNRF DPDRFSETRQ EDQVFKRNYL AFGWGAHQCV GQRYALNHLV 450
    LFIAMFSSLF DFKRLQSDGC DDIIYCPTIS PKDGCTVFLS KRIVTYPNL 499
    Length:499
    Mass (Da):56,346
    Last modified:August 1, 1998 - v1
    Checksum:i6CC7D1CBC0474348
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti18 – 181A → S in AAM62799. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB233425 Genomic DNA. Translation: BAE80316.1.
    AC004077 Genomic DNA. Translation: AAC26691.1.
    AC004481 Genomic DNA. Translation: AAM14945.1.
    CP002685 Genomic DNA. Translation: AEC08981.1.
    AY074867 mRNA. Translation: AAL75888.1.
    AY101543 mRNA. Translation: AAM26664.1.
    AY085577 mRNA. Translation: AAM62799.1.
    PIRiT02336.
    RefSeqiNP_180996.1. NM_129001.2.
    UniGeneiAt.27927.

    Genome annotation databases

    EnsemblPlantsiAT2G34490.1; AT2G34490.1; AT2G34490.
    GeneIDi818012.
    KEGGiath:AT2G34490.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB233425 Genomic DNA. Translation: BAE80316.1 .
    AC004077 Genomic DNA. Translation: AAC26691.1 .
    AC004481 Genomic DNA. Translation: AAM14945.1 .
    CP002685 Genomic DNA. Translation: AEC08981.1 .
    AY074867 mRNA. Translation: AAL75888.1 .
    AY101543 mRNA. Translation: AAM26664.1 .
    AY085577 mRNA. Translation: AAM62799.1 .
    PIRi T02336.
    RefSeqi NP_180996.1. NM_129001.2.
    UniGenei At.27927.

    3D structure databases

    ProteinModelPortali O64698.
    SMRi O64698. Positions 31-493.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G34490.1-P.

    Proteomic databases

    PaxDbi O64698.
    PRIDEi O64698.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G34490.1 ; AT2G34490.1 ; AT2G34490 .
    GeneIDi 818012.
    KEGGi ath:AT2G34490.

    Organism-specific databases

    GeneFarmi 1169. 94.
    TAIRi AT2G34490.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000237640.
    InParanoidi O64698.
    KOi K09832.
    OMAi AFPITPD.
    PhylomeDBi O64698.

    Enzyme and pathway databases

    UniPathwayi UPA00766 .
    BioCyci ARA:AT2G34490-MONOMER.
    MetaCyc:AT2G34490-MONOMER.

    Gene expression databases

    Genevestigatori O64698.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato."
      Morikawa T., Mizutani M., Aoki N., Watanabe B., Saga H., Saito S., Oikawa A., Suzuki H., Sakurai N., Shibata D., Wadano A., Sakata K., Ohta D.
      Plant Cell 18:1008-1022(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiC7102_ARATH
    AccessioniPrimary (citable) accession number: O64698
    Secondary accession number(s): Q8LE78
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 27, 2011
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Plants overexpressing CYP710A2 show higher levels of brassicasterol/crinosterol and stigmasterol, and lower levels of 24-epi-campesterol/campesterol and beta-sitosterol than the wild-type.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3