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O64698 (C7102_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 710A2

EC=1.14.-.-
Alternative name(s):
C-22 sterol desaturase
Gene names
Name:CYP710A2
Ordered Locus Names:At2g34490
ORF Names:F13P17.22
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Required to form the C-22 double bond in the sterol side chain. Possesses in vitro C-22 desaturase activity toward 24-epi-campesterol and beta-sitosterol and produces brassicasterol and stigmasterol, respectively. Ref.1

Cofactor

Heme group By similarity.

Pathway

Steroid biosynthesis; sterol biosynthesis.

Subcellular location

Membrane; Single-pass membrane protein Potential.

Tissue specificity

Expressed in the vascular tissues of roots, shoots, stems and leaves. Expressed in root tips, carpes, siliques and seeds. Ref.1

Disruption phenotype

No visible phenotype under normal growth conditions, but production of brassicasterol is abolished. Ref.1

Miscellaneous

Plants overexpressing CYP710A2 show higher levels of brassicasterol/crinosterol and stigmasterol, and lower levels of 24-epi-campesterol/campesterol and beta-sitosterol than the wild-type.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 499499Cytochrome P450 710A2
PRO_0000411206

Regions

Transmembrane5 – 2521Helical; Potential

Sites

Metal binding4391Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict181A → S in AAM62799. Ref.4

Sequences

Sequence LengthMass (Da)Tools
O64698 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 6CC7D1CBC0474348

FASTA49956,346
        10         20         30         40         50         60 
MVFSVSIFAS LAPYLVSALL LFFLIEQLSY LVKKRNLPGP LFVPPIIGNA ISLVRDPTSF 

        70         80         90        100        110        120 
WFKQSDTAGT SPGLAANYLI GKFIIYIRDT ELSHQIFSNV RLEAFHPLGH PFGKQLFGDH 

       130        140        150        160        170        180 
SLIYLFGEDH KTVRRHLAPN FTPKALSTYS DLQQIVMLRH LRQWEESFSG GTKPVSMRDL 

       190        200        210        220        230        240 
VRELNLETSQ TVFVGPYLDK EARNTFCTDY NLFNLGSMAL PINLPGFAFN KARRAVMNLE 

       250        260        270        280        290        300 
KTLSVCAGKS KKRMATGEEP TCLIDFWMHA FVTEIESGNP PPLHSEDEAI GGLLFDFLFA 

       310        320        330        340        350        360 
AQDASTSSLL WAVTFLESHP KVLSKVREEV AKIWSPQSGH LITADQLAEM KYTRAVAREV 

       370        380        390        400        410        420 
VRYRPPATMV PHIATNDFPL TESYTIPKGT IVFPSVFDAS FQGFTEPNRF DPDRFSETRQ 

       430        440        450        460        470        480 
EDQVFKRNYL AFGWGAHQCV GQRYALNHLV LFIAMFSSLF DFKRLQSDGC DDIIYCPTIS 

       490 
PKDGCTVFLS KRIVTYPNL 

« Hide

References

« Hide 'large scale' references
[1]"Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato."
Morikawa T., Mizutani M., Aoki N., Watanabe B., Saga H., Saito S., Oikawa A., Suzuki H., Sakurai N., Shibata D., Wadano A., Sakata K., Ohta D.
Plant Cell 18:1008-1022(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB233425 Genomic DNA. Translation: BAE80316.1.
AC004077 Genomic DNA. Translation: AAC26691.1.
AC004481 Genomic DNA. Translation: AAM14945.1.
CP002685 Genomic DNA. Translation: AEC08981.1.
AY074867 mRNA. Translation: AAL75888.1.
AY101543 mRNA. Translation: AAM26664.1.
AY085577 mRNA. Translation: AAM62799.1.
PIRT02336.
RefSeqNP_180996.1. NM_129001.2.
UniGeneAt.27927.

3D structure databases

ProteinModelPortalO64698.
SMRO64698. Positions 206-493.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G34490.1-P.

Proteomic databases

PaxDbO64698.
PRIDEO64698.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G34490.1; AT2G34490.1; AT2G34490.
GeneID818012.
KEGGath:AT2G34490.

Organism-specific databases

GeneFarm1169. 94.
TAIRAT2G34490.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000237640.
InParanoidO64698.
KOK09832.
OMAAFPITPD.
PhylomeDBO64698.
ProtClustDBCLSN2683644.

Enzyme and pathway databases

BioCycARA:AT2G34490-MONOMER.
MetaCyc:AT2G34490-MONOMER.
UniPathwayUPA00766.

Gene expression databases

GenevestigatorO64698.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC7102_ARATH
AccessionPrimary (citable) accession number: O64698
Secondary accession number(s): Q8LE78
Entry history
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: August 1, 1998
Last modified: March 19, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names