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Protein

Cytochrome P450 710A2

Gene

CYP710A2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required to form the C-22 double bond in the sterol side chain. Possesses in vitro C-22 desaturase activity toward 24-epi-campesterol and beta-sitosterol and produces brassicasterol and stigmasterol, respectively.1 Publication

Cofactori

hemeBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi439 – 4391Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. C-22 sterol desaturase activity Source: TAIR
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
  4. monooxygenase activity Source: UniProtKB-KW

GO - Biological processi

  1. sterol biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G34490-MONOMER.
MetaCyc:AT2G34490-MONOMER.
UniPathwayiUPA00766.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 710A2 (EC:1.14.-.-)
Alternative name(s):
C-22 sterol desaturase
Gene namesi
Name:CYP710A2
Ordered Locus Names:At2g34490
ORF Names:F13P17.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G34490.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei5 – 2521HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but production of brassicasterol is abolished.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 499499Cytochrome P450 710A2PRO_0000411206Add
BLAST

Proteomic databases

PaxDbiO64698.
PRIDEiO64698.

Expressioni

Tissue specificityi

Expressed in the vascular tissues of roots, shoots, stems and leaves. Expressed in root tips, carpes, siliques and seeds.1 Publication

Gene expression databases

GenevestigatoriO64698.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G34490.1-P.

Structurei

3D structure databases

ProteinModelPortaliO64698.
SMRiO64698. Positions 31-497.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000237640.
InParanoidiO64698.
KOiK09832.
OMAiFGAGPHD.
PhylomeDBiO64698.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O64698-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVFSVSIFAS LAPYLVSALL LFFLIEQLSY LVKKRNLPGP LFVPPIIGNA
60 70 80 90 100
ISLVRDPTSF WFKQSDTAGT SPGLAANYLI GKFIIYIRDT ELSHQIFSNV
110 120 130 140 150
RLEAFHPLGH PFGKQLFGDH SLIYLFGEDH KTVRRHLAPN FTPKALSTYS
160 170 180 190 200
DLQQIVMLRH LRQWEESFSG GTKPVSMRDL VRELNLETSQ TVFVGPYLDK
210 220 230 240 250
EARNTFCTDY NLFNLGSMAL PINLPGFAFN KARRAVMNLE KTLSVCAGKS
260 270 280 290 300
KKRMATGEEP TCLIDFWMHA FVTEIESGNP PPLHSEDEAI GGLLFDFLFA
310 320 330 340 350
AQDASTSSLL WAVTFLESHP KVLSKVREEV AKIWSPQSGH LITADQLAEM
360 370 380 390 400
KYTRAVAREV VRYRPPATMV PHIATNDFPL TESYTIPKGT IVFPSVFDAS
410 420 430 440 450
FQGFTEPNRF DPDRFSETRQ EDQVFKRNYL AFGWGAHQCV GQRYALNHLV
460 470 480 490
LFIAMFSSLF DFKRLQSDGC DDIIYCPTIS PKDGCTVFLS KRIVTYPNL
Length:499
Mass (Da):56,346
Last modified:August 1, 1998 - v1
Checksum:i6CC7D1CBC0474348
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181A → S in AAM62799 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB233425 Genomic DNA. Translation: BAE80316.1.
AC004077 Genomic DNA. Translation: AAC26691.1.
AC004481 Genomic DNA. Translation: AAM14945.1.
CP002685 Genomic DNA. Translation: AEC08981.1.
AY074867 mRNA. Translation: AAL75888.1.
AY101543 mRNA. Translation: AAM26664.1.
AY085577 mRNA. Translation: AAM62799.1.
PIRiT02336.
RefSeqiNP_180996.1. NM_129001.2.
UniGeneiAt.27927.

Genome annotation databases

EnsemblPlantsiAT2G34490.1; AT2G34490.1; AT2G34490.
GeneIDi818012.
KEGGiath:AT2G34490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB233425 Genomic DNA. Translation: BAE80316.1.
AC004077 Genomic DNA. Translation: AAC26691.1.
AC004481 Genomic DNA. Translation: AAM14945.1.
CP002685 Genomic DNA. Translation: AEC08981.1.
AY074867 mRNA. Translation: AAL75888.1.
AY101543 mRNA. Translation: AAM26664.1.
AY085577 mRNA. Translation: AAM62799.1.
PIRiT02336.
RefSeqiNP_180996.1. NM_129001.2.
UniGeneiAt.27927.

3D structure databases

ProteinModelPortaliO64698.
SMRiO64698. Positions 31-497.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G34490.1-P.

Proteomic databases

PaxDbiO64698.
PRIDEiO64698.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G34490.1; AT2G34490.1; AT2G34490.
GeneIDi818012.
KEGGiath:AT2G34490.

Organism-specific databases

GeneFarmi1169. 94.
TAIRiAT2G34490.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000237640.
InParanoidiO64698.
KOiK09832.
OMAiFGAGPHD.
PhylomeDBiO64698.

Enzyme and pathway databases

UniPathwayiUPA00766.
BioCyciARA:AT2G34490-MONOMER.
MetaCyc:AT2G34490-MONOMER.

Gene expression databases

GenevestigatoriO64698.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato."
    Morikawa T., Mizutani M., Aoki N., Watanabe B., Saga H., Saito S., Oikawa A., Suzuki H., Sakurai N., Shibata D., Wadano A., Sakata K., Ohta D.
    Plant Cell 18:1008-1022(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiC7102_ARATH
AccessioniPrimary (citable) accession number: O64698
Secondary accession number(s): Q8LE78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: August 1, 1998
Last modified: February 4, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants overexpressing CYP710A2 show higher levels of brassicasterol/crinosterol and stigmasterol, and lower levels of 24-epi-campesterol/campesterol and beta-sitosterol than the wild-type.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.