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Protein

Cytochrome P450 710A1

Gene

CYP710A1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required to form the C-22 double bond in the sterol side chain. Possesses in vitro C-22 desaturase activity toward beta-sitosterol and produces stigmasterol. No activity with campesterol.3 Publications

Catalytic activityi

Ergosta-5,7,24(28)-trien-3-beta-ol + NADPH + O2 = ergosta-5,7,22,24(28)-tetraen-3-beta-ol + NADP+ + 2 H2O.1 Publication

Cofactori

hemeBy similarity

Kineticsi

kcat is 0.53 min(-1) for beta-sisterol.1 Publication

  1. KM=1 µM for beta-sisterol1 Publication

    Pathwayi: sterol biosynthesis

    This protein is involved in the pathway sterol biosynthesis, which is part of Steroid biosynthesis.1 Publication
    View all proteins of this organism that are known to be involved in the pathway sterol biosynthesis and in Steroid biosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi434 – 4341Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    • C-22 sterol desaturase activity Source: TAIR
    • heme binding Source: InterPro
    • iron ion binding Source: InterPro
    • monooxygenase activity Source: UniProtKB-KW

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciARA:AT2G34500-MONOMER.
    MetaCyc:AT2G34500-MONOMER.
    UniPathwayiUPA00766.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 710A11 Publication (EC:1.14.19.411 Publication)
    Alternative name(s):
    C-22 sterol desaturase1 Publication
    Gene namesi
    Name:CYP710A11 Publication
    Ordered Locus Names:At2g34500Imported
    ORF Names:F13P17.35Imported
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 2

    Organism-specific databases

    TAIRiAT2G34500.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei5 – 2521HelicalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth condition, but upon infection with P.syringae, no accumulation of stigmasterol and increased resistance to the pathogen.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 495495Cytochrome P450 710A1PRO_0000411205Add
    BLAST

    Proteomic databases

    PaxDbiO64697.

    Expressioni

    Tissue specificityi

    Expressed in the vascular tissues of roots, shoots and leaves. Expressed in root tips and sepals. Very low expression in stems and siliques.1 Publication

    Inductioni

    By infection with Pseudomonas syringae.1 Publication

    Gene expression databases

    GenevisibleiO64697. AT.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G34500.1.

    Structurei

    3D structure databases

    ProteinModelPortaliO64697.
    SMRiO64697. Positions 31-487.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG0157. Eukaryota.
    COG2124. LUCA.
    HOGENOMiHOG000237640.
    InParanoidiO64697.
    KOiK09832.
    OMAiFQVTAQR.
    OrthoDBiEOG093607IL.
    PhylomeDBiO64697.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O64697-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVFSVSIFAS LAPYLISAFL LFLLVEQLSY LFKKRNIPGP FFVPPIIGNA
    60 70 80 90 100
    VALVRDPTSF WDKQSSTANI SGLSANYLIG KFIVYIRDTE LSHQIFSNVR
    110 120 130 140 150
    PDAFHLIGHP FGKKLFGDHN LIYMFGEDHK SVRRQLAPNF TPKALSTYSA
    160 170 180 190 200
    LQQLVILRHL RQWEGSTSGG SRPVSLRQLV RELNLETSQT VFVGPYLDKE
    210 220 230 240 250
    AKNRFRTDYN LFNLGSMALP IDLPGFAFGE ARRAVKRLGE TLGICAGKSK
    260 270 280 290 300
    ARMAAGEEPA CLIDFWMQAI VAENPQPPHS GDEEIGGLLF DFLFAAQDAS
    310 320 330 340 350
    TSSLLWAVTL LDSEPEVLNR VREEVAKIWS PESNALITVD QLAEMKYTRS
    360 370 380 390 400
    VAREVIRYRP PATMVPHVAA IDFPLTETYT IPKGTIVFPS VFDSSFQGFT
    410 420 430 440 450
    EPDRFDPDRF SETRQEDQVF KRNFLAFGWG PHQCVGQRYA LNHLVLFIAM
    460 470 480 490
    FSSLLDFKRL RSDGCDEIVY CPTISPKDGC TVFLSRRVAK YPNFS
    Length:495
    Mass (Da):55,724
    Last modified:August 1, 1998 - v1
    Checksum:i61F428F868D290B1
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB219423 Genomic DNA. Translation: BAE71351.1.
    AC004077 Genomic DNA. Translation: AAC26690.1.
    AC004481 Genomic DNA. Translation: AAM14944.1.
    CP002685 Genomic DNA. Translation: AEC08982.1.
    BT002069 mRNA. Translation: AAN72080.1.
    BT010554 mRNA. Translation: AAQ65177.1.
    PIRiT02337.
    RefSeqiNP_180997.1. NM_129002.2.
    UniGeneiAt.27806.
    At.67747.

    Genome annotation databases

    EnsemblPlantsiAT2G34500.1; AT2G34500.1; AT2G34500.
    GeneIDi818013.
    GrameneiAT2G34500.1; AT2G34500.1; AT2G34500.
    KEGGiath:AT2G34500.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB219423 Genomic DNA. Translation: BAE71351.1.
    AC004077 Genomic DNA. Translation: AAC26690.1.
    AC004481 Genomic DNA. Translation: AAM14944.1.
    CP002685 Genomic DNA. Translation: AEC08982.1.
    BT002069 mRNA. Translation: AAN72080.1.
    BT010554 mRNA. Translation: AAQ65177.1.
    PIRiT02337.
    RefSeqiNP_180997.1. NM_129002.2.
    UniGeneiAt.27806.
    At.67747.

    3D structure databases

    ProteinModelPortaliO64697.
    SMRiO64697. Positions 31-487.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT2G34500.1.

    Proteomic databases

    PaxDbiO64697.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT2G34500.1; AT2G34500.1; AT2G34500.
    GeneIDi818013.
    GrameneiAT2G34500.1; AT2G34500.1; AT2G34500.
    KEGGiath:AT2G34500.

    Organism-specific databases

    TAIRiAT2G34500.

    Phylogenomic databases

    eggNOGiKOG0157. Eukaryota.
    COG2124. LUCA.
    HOGENOMiHOG000237640.
    InParanoidiO64697.
    KOiK09832.
    OMAiFQVTAQR.
    OrthoDBiEOG093607IL.
    PhylomeDBiO64697.

    Enzyme and pathway databases

    UniPathwayiUPA00766.
    BioCyciARA:AT2G34500-MONOMER.
    MetaCyc:AT2G34500-MONOMER.

    Miscellaneous databases

    PROiO64697.

    Gene expression databases

    GenevisibleiO64697. AT.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiC7101_ARATH
    AccessioniPrimary (citable) accession number: O64697
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 27, 2011
    Last sequence update: August 1, 1998
    Last modified: September 7, 2016
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Plants overexpressing CYP710A1 show higher levels of stigmasterol and lower levels of beta-sitosterol than the wild-type.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.