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Protein

Cytochrome P450 710A1

Gene

CYP710A1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required to form the C-22 double bond in the sterol side chain. Possesses in vitro C-22 desaturase activity toward beta-sitosterol and produces stigmasterol. No activity with campesterol.3 Publications

Miscellaneous

Plants overexpressing CYP710A1 show higher levels of stigmasterol and lower levels of beta-sitosterol than the wild-type.1 Publication

Catalytic activityi

Ergosta-5,7,24(28)-trien-3-beta-ol + NADPH + O2 = ergosta-5,7,22,24(28)-tetraen-3-beta-ol + NADP+ + 2 H2O.1 Publication

Cofactori

hemeBy similarity

Kineticsi

kcat is 0.53 min(-1) for beta-sisterol.1 Publication
  1. KM=1 µM for beta-sisterol1 Publication

    Pathwayi: sterol biosynthesis

    This protein is involved in the pathway sterol biosynthesis, which is part of Steroid biosynthesis.1 Publication
    View all proteins of this organism that are known to be involved in the pathway sterol biosynthesis and in Steroid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi434Iron (heme axial ligand)By similarity1

    GO - Molecular functioni

    • C-22 sterol desaturase activity Source: TAIR
    • heme binding Source: InterPro
    • iron ion binding Source: InterPro
    • monooxygenase activity Source: UniProtKB-KW

    GO - Biological processi

    Keywordsi

    Molecular functionMonooxygenase, Oxidoreductase
    Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
    LigandHeme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciARA:AT2G34500-MONOMER
    MetaCyc:AT2G34500-MONOMER
    UniPathwayiUPA00766

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 710A11 Publication (EC:1.14.19.411 Publication)
    Alternative name(s):
    C-22 sterol desaturase1 Publication
    Gene namesi
    Name:CYP710A11 Publication
    Ordered Locus Names:At2g34500Imported
    ORF Names:F13P17.35Imported
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 2

    Organism-specific databases

    AraportiAT2G34500
    TAIRilocus:2040939 AT2G34500

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei5 – 25HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth condition, but upon infection with P.syringae, no accumulation of stigmasterol and increased resistance to the pathogen.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004112051 – 495Cytochrome P450 710A1Add BLAST495

    Proteomic databases

    PaxDbiO64697
    PRIDEiO64697

    Expressioni

    Tissue specificityi

    Expressed in the vascular tissues of roots, shoots and leaves. Expressed in root tips and sepals. Very low expression in stems and siliques.1 Publication

    Inductioni

    By infection with Pseudomonas syringae.1 Publication

    Gene expression databases

    ExpressionAtlasiO64697 baseline and differential
    GenevisibleiO64697 AT

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G34500.1

    Structurei

    3D structure databases

    ProteinModelPortaliO64697
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG0157 Eukaryota
    COG2124 LUCA
    HOGENOMiHOG000237640
    InParanoidiO64697
    KOiK09832
    OMAiATIFPMD
    OrthoDBiEOG093607IL
    PhylomeDBiO64697

    Family and domain databases

    Gene3Di1.10.630.10, 1 hit
    InterProiView protein in InterPro
    IPR001128 Cyt_P450
    IPR017972 Cyt_P450_CS
    IPR002401 Cyt_P450_E_grp-I
    IPR036396 Cyt_P450_sf
    PfamiView protein in Pfam
    PF00067 p450, 1 hit
    PRINTSiPR00463 EP450I
    PR00385 P450
    SUPFAMiSSF48264 SSF48264, 1 hit
    PROSITEiView protein in PROSITE
    PS00086 CYTOCHROME_P450, 1 hit

    Sequencei

    Sequence statusi: Complete.

    O64697-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVFSVSIFAS LAPYLISAFL LFLLVEQLSY LFKKRNIPGP FFVPPIIGNA
    60 70 80 90 100
    VALVRDPTSF WDKQSSTANI SGLSANYLIG KFIVYIRDTE LSHQIFSNVR
    110 120 130 140 150
    PDAFHLIGHP FGKKLFGDHN LIYMFGEDHK SVRRQLAPNF TPKALSTYSA
    160 170 180 190 200
    LQQLVILRHL RQWEGSTSGG SRPVSLRQLV RELNLETSQT VFVGPYLDKE
    210 220 230 240 250
    AKNRFRTDYN LFNLGSMALP IDLPGFAFGE ARRAVKRLGE TLGICAGKSK
    260 270 280 290 300
    ARMAAGEEPA CLIDFWMQAI VAENPQPPHS GDEEIGGLLF DFLFAAQDAS
    310 320 330 340 350
    TSSLLWAVTL LDSEPEVLNR VREEVAKIWS PESNALITVD QLAEMKYTRS
    360 370 380 390 400
    VAREVIRYRP PATMVPHVAA IDFPLTETYT IPKGTIVFPS VFDSSFQGFT
    410 420 430 440 450
    EPDRFDPDRF SETRQEDQVF KRNFLAFGWG PHQCVGQRYA LNHLVLFIAM
    460 470 480 490
    FSSLLDFKRL RSDGCDEIVY CPTISPKDGC TVFLSRRVAK YPNFS
    Length:495
    Mass (Da):55,724
    Last modified:August 1, 1998 - v1
    Checksum:i61F428F868D290B1
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB219423 Genomic DNA Translation: BAE71351.1
    AC004077 Genomic DNA Translation: AAC26690.1
    AC004481 Genomic DNA Translation: AAM14944.1
    CP002685 Genomic DNA Translation: AEC08982.1
    BT002069 mRNA Translation: AAN72080.1
    BT010554 mRNA Translation: AAQ65177.1
    PIRiT02337
    RefSeqiNP_180997.1, NM_129002.3
    UniGeneiAt.27806
    At.67747

    Genome annotation databases

    EnsemblPlantsiAT2G34500.1; AT2G34500.1; AT2G34500
    GeneIDi818013
    GrameneiAT2G34500.1; AT2G34500.1; AT2G34500
    KEGGiath:AT2G34500

    Similar proteinsi

    Entry informationi

    Entry nameiC7101_ARATH
    AccessioniPrimary (citable) accession number: O64697
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 27, 2011
    Last sequence update: August 1, 1998
    Last modified: May 23, 2018
    This is version 127 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health