O64692 (G2OX3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gibberellin 2-beta-dioxygenase 3
EC=1.14.11.13

Alternative name(s):
GA 2-oxidase 3
Gibberellin 2-beta-hydroxylase 3
Gibberellin 2-oxidase 3

Gene names

Name:	GA2OX3
Ordered Locus Names:	At2g34555
ORF Names:	T31E10.11

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the 2-beta-hydroxylation of several biologically active gibberellins, leading to the homeostatic regulation of their endogenous level. Catabolism of gibberellins (GAs) plays a central role in plant development. Converts GA9/GA20 to GA51/GA29 and GA4/GA1 to GA34/GA8. Ref.1
Catalytic activity	Gibberellin 1 + 2-oxoglutarate + O2 = 2-beta-hydroxygibberellin 1 + succinate + CO2.
Cofactor	Iron By similarity.
Pathway	Plant hormone biosynthesis; gibberellin biosynthesis.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. GA2OX subfamily. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	gibberellin 2-beta-dioxygenase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Gibberellin 2-beta-dioxygenase 3		PRO_0000067307
Regions
	Domain	175 – 278	104	Fe2OG dioxygenase
Sites
	Active site	269	1	Potential
	Metal binding	202	1	Iron By similarity
	Metal binding	204	1	Iron By similarity
	Metal binding	259	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	O64692 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 181F6EAA1EE1C331	FASTA	335	38,216
	10 20 30 40 50 60 MVIVLQPASF DSNLYVNPKC KPRPVLIPVI DLTDSDAKTQ IVKACEEFGF FKVINHGVRP 70 80 90 100 110 120 DLLTQLEQEA INFFALHHSL KDKAGPPDPF GYGTKRIGPN GDLGWLEYIL LNANLCLESH 130 140 150 160 170 180 KTTAIFRHTP AIFREAVEEY IKEMKRMSSK FLEMVEEELK IEPKEKLSRL VKVKESDSCL 190 200 210 220 230 240 RMNHYPEKEE TPVKEEIGFG EHTDPQLISL LRSNDTEGLQ ICVKDGTWVD VTPDHSSFFV 250 260 270 280 290 300 LVGDTLQVMT NGRFKSVKHR VVTNTKRSRI SMIYFAGPPL SEKIAPLSCL VPKQDDCLYN 310 320 330 EFTWSQYKLS AYKTKLGDYR LGLFEKRPPF SLSNV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and functional expression of gibberellin 2-oxidases, multifunctional enzymes involved in gibberellin deactivation." Thomas S.G., Phillips A.L., Hedden P. Proc. Natl. Acad. Sci. U.S.A. 96:4698-4703(1999) [PubMed: 10200325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ132437 mRNA. Translation: CAB41009.1. AC004077 Genomic DNA. Translation: AAM14908.1. CP002685 Genomic DNA. Translation: AEC08989.1.
IPI	IPI00546397.
PIR	T52577.
RefSeq	NP_181002.1. NM_129007.1.
UniGene	At.413.
3D structure databases
ProteinModelPortal	O64692.
SMR	O64692. Positions 23-315.
ModBase	Search...
Protein-protein interaction databases
STRING	O64692.
Proteomic databases
PRIDE	O64692.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G34555.1; AT2G34555.1; AT2G34555.
GeneID	818019.
GenomeReviews	Gene locus AT2G34555 in contig CT485783_GR.
KEGG	ath:AT2G34555.
NMPDR	fig|3702.1.peg.10550.
Organism-specific databases
GeneFarm	3784. 382.
TAIR	At2g34555.
Phylogenomic databases
eggNOG	KOG0143.
GeneTree	EPGT00050000009703.
HOGENOM	HBG590288.
InParanoid	O64692.
OMA	AIFREAV.
PhylomeDB	O64692.
ProtClustDB	PLN02156.
Enzyme and pathway databases
BioCyc	ARA:AT2G34555-MONOMER. MetaCyc:AT2G34555-MONOMER.
Gene expression databases
ArrayExpress	O64692.
Genevestigator	O64692.
GermOnline	AT2G34555. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view]
KO	K04125.
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G2OX3_ARATH

Accession

Primary (citable) accession number: O64692

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2004
Last sequence update:	August 1, 1998
Last modified:	December 14, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program