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Reviewed, UniProtKB/Swiss-Prot O64692 (G2OX3_ARATH)

Last modified November 25, 2008. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Gibberellin 2-beta-dioxygenase 3
    EC=1.14.11.13
Alternative name(s):
    Gibberellin 2-beta-hydroxylase 3
    Gibberellin 2-oxidase 3
    GA 2-oxidase 3
Gene names
Name: GA2OX3
Ordered Locus Names: At2g34555
ORF Names: T31E10.11
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids IIBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length335 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Catalyzes the 2-beta-hydroxylation of several biologically active gibberellins, leading to the homeostatic regulation of their endogenous level. Catabolism of gibberellins (GAs) plays a central role in plant development. Converts GA9/GA20 to GA51/GA29 and GA4/GA1 to GA34/GA8.

Catalytic activity

Gibberellin 1 + 2-oxoglutarate + O(2) = 2-beta-hydroxygibberellin 1 + succinate + CO(2).

Cofactor

Iron By similarity.

Pathway

Plant hormone biosynthesis; gibberellin biosynthesis.

Sequence similarities

Belongs to the iron/ascorbate-dependent oxidoreductase family. GA2OX subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 335335Gibberellin 2-beta-dioxygenase 3
PRO_0000067307

Sites

Active site2691 Potential
Metal binding2021Iron By similarity
Metal binding2041Iron By similarity
Metal binding2591Iron By similarity

Sequences

Sequence LengthMass (Da)Tools
O64692-1 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 181F6EAA1EE1C331

FASTA33538,216
        10         20         30         40         50         60 
MVIVLQPASF DSNLYVNPKC KPRPVLIPVI DLTDSDAKTQ IVKACEEFGF FKVINHGVRP 

        70         80         90        100        110        120 
DLLTQLEQEA INFFALHHSL KDKAGPPDPF GYGTKRIGPN GDLGWLEYIL LNANLCLESH 

       130        140        150        160        170        180 
KTTAIFRHTP AIFREAVEEY IKEMKRMSSK FLEMVEEELK IEPKEKLSRL VKVKESDSCL 

       190        200        210        220        230        240 
RMNHYPEKEE TPVKEEIGFG EHTDPQLISL LRSNDTEGLQ ICVKDGTWVD VTPDHSSFFV 

       250        260        270        280        290        300 
LVGDTLQVMT NGRFKSVKHR VVTNTKRSRI SMIYFAGPPL SEKIAPLSCL VPKQDDCLYN 

       310        320        330 
EFTWSQYKLS AYKTKLGDYR LGLFEKRPPF SLSNV 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and functional expression of gibberellin 2-oxidases, multifunctional enzymes involved in gibberellin deactivation."
Thomas S.G., Phillips A.L., Hedden P.
Proc. Natl. Acad. Sci. U.S.A. 96:4698-4703(1999) [PubMed: 10200325] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed: 10617197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.

Cross-references

Sequence databases

AJ132437 mRNA. Translation: CAB41009.1.
AC004077 Genomic DNA. Translation: AAM14908.1.
PIRT52577.
RefSeqNP_181002.1.
UniGeneAt.413

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID818019.
GenomeReviewsGene locus AT2G34555 in contig CT485783_GR.
KEGGath:AT2G34555.
NMPDRfig|3702.1.peg.10550.

Organism-specific databases

GeneFarm3784. 382.
TAIRAt2g34555.

Enzyme and pathway databases

BioCycMetaCyc:AT2G34555-MON.

Gene expression databases

ArrayExpressO64692.
GermOnlineAT2G34555. Arabidopsis thaliana.

Family and domain databases

InterProIPR005123. 2OG-FeII_Oase.
IPR002283. Isopenicillin-N_synthase.
[Graphical view]
PfamPF03171. 2OG-FeII_Oxy. 1 hit.
[Graphical view]
PRINTSPR00682. IPNSYNTHASE.
ProtoNetSearch...

Entry information

Entry nameG2OX3_ARATH
AccessionPrimary (citable) accession number: O64692
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: August 1, 1998
Last modified: November 25, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents