Reviewed,
UniProtKB/Swiss-Prot O64692 (G2OX3_ARATH)
Last modified
February 9, 2010.
Version 64.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Gibberellin 2-beta-dioxygenase 3 EC=1.14.11.13 Alternative name(s): Gibberellin 2-beta-hydroxylase 3 Gibberellin 2-oxidase 3 GA 2-oxidase 3 | ||||||
| Gene names |
| ||||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 335 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Catalyzes the 2-beta-hydroxylation of several biologically active gibberellins, leading to the homeostatic regulation of their endogenous level. Catabolism of gibberellins (GAs) plays a central role in plant development. Converts GA9/GA20 to GA51/GA29 and GA4/GA1 to GA34/GA8. Ref.1 |
| Catalytic activity | Gibberellin 1 + 2-oxoglutarate + O2 = 2-beta-hydroxygibberellin 1 + succinate + CO2. |
| Cofactor | Iron By similarity. |
| Pathway | |
| Sequence similarities | Belongs to the iron/ascorbate-dependent oxidoreductase family. GA2OX subfamily. Contains 1 Fe2OG dioxygenase domain. |
Ontologies
| Keywords | |
|---|---|
| Ligand | Iron Metal-binding |
| Molecular function | Dioxygenase Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | gibberellin 2-beta-dioxygenase activity Inferred from electronic annotation. Source: EC iron ion bindingInferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygenInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 335 | 335 | Gibberellin 2-beta-dioxygenase 3 | PRO_0000067307 | |||||
Regions | |||||||||
| Domain | 175 – 278 | 104 | Fe2OG dioxygenase | ||||||
Sites | |||||||||
| Active site | 269 | 1 | Potential | ||||||
| Metal binding | 202 | 1 | Iron By similarity | ||||||
| Metal binding | 204 | 1 | Iron By similarity | ||||||
| Metal binding | 259 | 1 | Iron By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular cloning and functional expression of gibberellin 2-oxidases, multifunctional enzymes involved in gibberellin deactivation." Thomas S.G., Phillips A.L., Hedden P. Proc. Natl. Acad. Sci. U.S.A. 96:4698-4703(1999) [PubMed: 10200325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: cv. Columbia. |
| [2] | "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C.Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AJ132437 mRNA. Translation: CAB41009.1. AC004077 Genomic DNA. Translation: AAM14908.1. |
| IPI | IPI00546397. |
| PIR | T52577. |
| RefSeq | NP_181002.1. |
| UniGene | At.413 |
3D structure databases | |
| SMR | O64692. Positions 26-308. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | O64692. |
Proteomic databases | |
| PRIDE | O64692. |
Genome annotation databases | |
| GeneID | 818019. |
| GenomeReviews | Gene locus AT2G34555 in contig CT485783_GR. |
| KEGG | ath:AT2G34555. |
| NMPDR | fig|3702.1.peg.10550. |
Organism-specific databases | |
| GeneFarm | 3784. 382. |
| TAIR | At2g34555. |
Phylogenomic databases | |
| eggNOG | KOG0143. |
| HOGENOM | HBG590288. |
| InParanoid | O64692. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:AT2G34555-MONOMER. |
| BRENDA | 1.14.11.13. 302. |
Gene expression databases | |
| ArrayExpress | O64692. |
| Genevestigator | O64692. |
| GermOnline | AT2G34555. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view] |
| Pfam | PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view] |
| PROSITE | PS51471. FE2OG_OXY. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | G2OX3_ARATH | ||||||||
| Accession | Primary (citable) accession number: O64692 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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