Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Presenilin-like protein At1g08700

Gene

At1g08700

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei190 – 1901By similarity
Active sitei374 – 3741By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Notch signaling pathway

Protein family/group databases

MEROPSiA22.A01.

Names & Taxonomyi

Protein namesi
Recommended name:
Presenilin-like protein At1g08700
Gene namesi
Ordered Locus Names:At1g08700
ORF Names:F22O13.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G08700.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence analysis
Transmembranei9 – 2921HelicalSequence analysisAdd
BLAST
Topological domaini30 – 6839LumenalSequence analysisAdd
BLAST
Transmembranei69 – 8921HelicalSequence analysisAdd
BLAST
Topological domaini90 – 10314CytoplasmicSequence analysisAdd
BLAST
Transmembranei104 – 12421HelicalSequence analysisAdd
BLAST
Topological domaini125 – 1328LumenalSequence analysis
Transmembranei133 – 15321HelicalSequence analysisAdd
BLAST
Topological domaini154 – 1596CytoplasmicSequence analysis
Transmembranei160 – 18021HelicalSequence analysisAdd
BLAST
Transmembranei181 – 20121HelicalSequence analysisAdd
BLAST
Topological domaini202 – 369168CytoplasmicSequence analysisAdd
BLAST
Transmembranei370 – 39021HelicalSequence analysisAdd
BLAST
Topological domaini391 – 3922LumenalSequence analysis
Transmembranei393 – 41321HelicalSequence analysisAdd
BLAST
Topological domaini414 – 4174CytoplasmicSequence analysis
Intramembranei418 – 43821HelicalSequence analysisAdd
BLAST
Topological domaini439 – 45315CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • Golgi-associated vesicle Source: TAIR
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of plasma membrane Source: GO_Central
  • intracellular membrane-bounded organelle Source: TAIR
  • nucleus Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 453453Presenilin-like protein At1g08700PRO_0000073905Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei296 – 2961PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO64668.
PRIDEiO64668.

PTM databases

iPTMnetiO64668.

Expressioni

Gene expression databases

ExpressionAtlasiO64668. baseline and differential.
GenevisibleiO64668. AT.

Interactioni

Subunit structurei

Homodimer. Probable component of the gamma-secretase complex, a complex composed of a presenilin homodimer, nicastrin, APH1 and PEN2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi22632. 3 interactions.
STRINGi3702.AT1G08700.1.

Structurei

3D structure databases

ProteinModelPortaliO64668.
SMRiO64668. Positions 71-452.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi419 – 4213PAL

Domaini

The PAL motif is required for normal active site conformation.By similarity

Sequence similaritiesi

Belongs to the peptidase A22A family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2736. Eukaryota.
ENOG410XPZD. LUCA.
HOGENOMiHOG000240228.
InParanoidiO64668.
KOiK04505.
OMAiDEELPAM.
OrthoDBiEOG09360DOM.
PhylomeDBiO64668.

Family and domain databases

InterProiIPR001108. Peptidase_A22A.
IPR006639. Preselin/SPP.
[Graphical view]
PANTHERiPTHR10202. PTHR10202. 1 hit.
PfamiPF01080. Presenilin. 1 hit.
[Graphical view]
PRINTSiPR01072. PRESENILIN.
SMARTiSM00730. PSN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O64668-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESSILDSLG VEIIGVMAPV SICMFLVVLL TYSLSVTSDP QIRSAANLIY
60 70 80 90 100
IENPSDSTTV KLEGSLANAI VFVVLIAAVT FILVLLFYYN FTNFLKHYMR
110 120 130 140 150
FSAFFVLGTM GGAIFLSIIQ HFSIPVDSIT CFILLFNFTI LGTLSVFAGG
160 170 180 190 200
IPIVLRQCYM VVMGIVVAAW FTKLPEWTTW FILVALALYD LVAVLAPGGP
210 220 230 240 250
LKLLVELASS RDEELPAMVY EARPTVSSGN QRRNRGSSLR ALVGGGGVSD
260 270 280 290 300
SGSVELQAVR NHDVNQLGRE NSHNMDYNAI AVRDIDNVDD GIGNGSRGGL
310 320 330 340 350
ERSPLVGSPS ASEHSTSVGT RGNMEDRESV MDEEMSPLVE LMGWGDNREE
360 370 380 390 400
ARGLEESDNV VDISNRGIKL GLGDFIFYSV LVGRAAMYDL MTVYACYLAI
410 420 430 440 450
ISGLGCTLIL LSVYNRALPA LPISIMLGVV FYFLTRLLME PFVVGVTTNL

MMF
Length:453
Mass (Da):49,308
Last modified:August 1, 1998 - v1
Checksum:i7FBDD9B86D97955E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti24 – 241M → I in AAM65750 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003981 Genomic DNA. Translation: AAF99776.1.
CP002684 Genomic DNA. Translation: AEE28336.1.
AY058880 mRNA. Translation: AAL24266.1.
AY088208 mRNA. Translation: AAM65750.1.
PIRiT00724.
RefSeqiNP_172346.1. NM_100743.3.
UniGeneiAt.26647.

Genome annotation databases

EnsemblPlantsiAT1G08700.1; AT1G08700.1; AT1G08700.
GeneIDi837391.
GrameneiAT1G08700.1; AT1G08700.1; AT1G08700.
KEGGiath:AT1G08700.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003981 Genomic DNA. Translation: AAF99776.1.
CP002684 Genomic DNA. Translation: AEE28336.1.
AY058880 mRNA. Translation: AAL24266.1.
AY088208 mRNA. Translation: AAM65750.1.
PIRiT00724.
RefSeqiNP_172346.1. NM_100743.3.
UniGeneiAt.26647.

3D structure databases

ProteinModelPortaliO64668.
SMRiO64668. Positions 71-452.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22632. 3 interactions.
STRINGi3702.AT1G08700.1.

Protein family/group databases

MEROPSiA22.A01.

PTM databases

iPTMnetiO64668.

Proteomic databases

PaxDbiO64668.
PRIDEiO64668.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G08700.1; AT1G08700.1; AT1G08700.
GeneIDi837391.
GrameneiAT1G08700.1; AT1G08700.1; AT1G08700.
KEGGiath:AT1G08700.

Organism-specific databases

TAIRiAT1G08700.

Phylogenomic databases

eggNOGiKOG2736. Eukaryota.
ENOG410XPZD. LUCA.
HOGENOMiHOG000240228.
InParanoidiO64668.
KOiK04505.
OMAiDEELPAM.
OrthoDBiEOG09360DOM.
PhylomeDBiO64668.

Miscellaneous databases

PROiO64668.

Gene expression databases

ExpressionAtlasiO64668. baseline and differential.
GenevisibleiO64668. AT.

Family and domain databases

InterProiIPR001108. Peptidase_A22A.
IPR006639. Preselin/SPP.
[Graphical view]
PANTHERiPTHR10202. PTHR10202. 1 hit.
PfamiPF01080. Presenilin. 1 hit.
[Graphical view]
PRINTSiPR01072. PRESENILIN.
SMARTiSM00730. PSN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSNA_ARATH
AccessioniPrimary (citable) accession number: O64668
Secondary accession number(s): Q8L9V0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.