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Protein

Presenilin-like protein At1g08700

Gene

At1g08700

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei190By similarity1
Active sitei374By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Notch signaling pathway

Enzyme and pathway databases

ReactomeiR-ATH-6798695. Neutrophil degranulation.

Protein family/group databases

MEROPSiA22.A01.

Names & Taxonomyi

Protein namesi
Recommended name:
Presenilin-like protein At1g08700
Gene namesi
Ordered Locus Names:At1g08700
ORF Names:F22O13.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G08700.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Topological domaini30 – 68LumenalSequence analysisAdd BLAST39
Transmembranei69 – 89HelicalSequence analysisAdd BLAST21
Topological domaini90 – 103CytoplasmicSequence analysisAdd BLAST14
Transmembranei104 – 124HelicalSequence analysisAdd BLAST21
Topological domaini125 – 132LumenalSequence analysis8
Transmembranei133 – 153HelicalSequence analysisAdd BLAST21
Topological domaini154 – 159CytoplasmicSequence analysis6
Transmembranei160 – 180HelicalSequence analysisAdd BLAST21
Transmembranei181 – 201HelicalSequence analysisAdd BLAST21
Topological domaini202 – 369CytoplasmicSequence analysisAdd BLAST168
Transmembranei370 – 390HelicalSequence analysisAdd BLAST21
Topological domaini391 – 392LumenalSequence analysis2
Transmembranei393 – 413HelicalSequence analysisAdd BLAST21
Topological domaini414 – 417CytoplasmicSequence analysis4
Intramembranei418 – 438HelicalSequence analysisAdd BLAST21
Topological domaini439 – 453CytoplasmicSequence analysisAdd BLAST15

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • Golgi-associated vesicle Source: TAIR
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of plasma membrane Source: GO_Central
  • intracellular membrane-bounded organelle Source: TAIR
  • nucleus Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000739051 – 453Presenilin-like protein At1g08700Add BLAST453

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei296PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO64668.
PRIDEiO64668.

PTM databases

iPTMnetiO64668.

Expressioni

Gene expression databases

ExpressionAtlasiO64668. baseline and differential.
GenevisibleiO64668. AT.

Interactioni

Subunit structurei

Homodimer. Probable component of the gamma-secretase complex, a complex composed of a presenilin homodimer, nicastrin, APH1 and PEN2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi22632. 3 interactors.
STRINGi3702.AT1G08700.1.

Structurei

3D structure databases

ProteinModelPortaliO64668.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi419 – 421PAL3

Domaini

The PAL motif is required for normal active site conformation.By similarity

Sequence similaritiesi

Belongs to the peptidase A22A family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2736. Eukaryota.
ENOG410XPZD. LUCA.
HOGENOMiHOG000240228.
InParanoidiO64668.
KOiK04505.
OMAiDEELPAM.
OrthoDBiEOG09360DOM.
PhylomeDBiO64668.

Family and domain databases

InterProiIPR001108. Peptidase_A22A.
IPR006639. Preselin/SPP.
[Graphical view]
PANTHERiPTHR10202. PTHR10202. 1 hit.
PfamiPF01080. Presenilin. 1 hit.
[Graphical view]
PRINTSiPR01072. PRESENILIN.
SMARTiSM00730. PSN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O64668-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESSILDSLG VEIIGVMAPV SICMFLVVLL TYSLSVTSDP QIRSAANLIY
60 70 80 90 100
IENPSDSTTV KLEGSLANAI VFVVLIAAVT FILVLLFYYN FTNFLKHYMR
110 120 130 140 150
FSAFFVLGTM GGAIFLSIIQ HFSIPVDSIT CFILLFNFTI LGTLSVFAGG
160 170 180 190 200
IPIVLRQCYM VVMGIVVAAW FTKLPEWTTW FILVALALYD LVAVLAPGGP
210 220 230 240 250
LKLLVELASS RDEELPAMVY EARPTVSSGN QRRNRGSSLR ALVGGGGVSD
260 270 280 290 300
SGSVELQAVR NHDVNQLGRE NSHNMDYNAI AVRDIDNVDD GIGNGSRGGL
310 320 330 340 350
ERSPLVGSPS ASEHSTSVGT RGNMEDRESV MDEEMSPLVE LMGWGDNREE
360 370 380 390 400
ARGLEESDNV VDISNRGIKL GLGDFIFYSV LVGRAAMYDL MTVYACYLAI
410 420 430 440 450
ISGLGCTLIL LSVYNRALPA LPISIMLGVV FYFLTRLLME PFVVGVTTNL

MMF
Length:453
Mass (Da):49,308
Last modified:August 1, 1998 - v1
Checksum:i7FBDD9B86D97955E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24M → I in AAM65750 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003981 Genomic DNA. Translation: AAF99776.1.
CP002684 Genomic DNA. Translation: AEE28336.1.
AY058880 mRNA. Translation: AAL24266.1.
AY088208 mRNA. Translation: AAM65750.1.
PIRiT00724.
RefSeqiNP_172346.1. NM_100743.4.
UniGeneiAt.26647.

Genome annotation databases

EnsemblPlantsiAT1G08700.1; AT1G08700.1; AT1G08700.
GeneIDi837391.
GrameneiAT1G08700.1; AT1G08700.1; AT1G08700.
KEGGiath:AT1G08700.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003981 Genomic DNA. Translation: AAF99776.1.
CP002684 Genomic DNA. Translation: AEE28336.1.
AY058880 mRNA. Translation: AAL24266.1.
AY088208 mRNA. Translation: AAM65750.1.
PIRiT00724.
RefSeqiNP_172346.1. NM_100743.4.
UniGeneiAt.26647.

3D structure databases

ProteinModelPortaliO64668.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22632. 3 interactors.
STRINGi3702.AT1G08700.1.

Protein family/group databases

MEROPSiA22.A01.

PTM databases

iPTMnetiO64668.

Proteomic databases

PaxDbiO64668.
PRIDEiO64668.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G08700.1; AT1G08700.1; AT1G08700.
GeneIDi837391.
GrameneiAT1G08700.1; AT1G08700.1; AT1G08700.
KEGGiath:AT1G08700.

Organism-specific databases

TAIRiAT1G08700.

Phylogenomic databases

eggNOGiKOG2736. Eukaryota.
ENOG410XPZD. LUCA.
HOGENOMiHOG000240228.
InParanoidiO64668.
KOiK04505.
OMAiDEELPAM.
OrthoDBiEOG09360DOM.
PhylomeDBiO64668.

Enzyme and pathway databases

ReactomeiR-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiO64668.

Gene expression databases

ExpressionAtlasiO64668. baseline and differential.
GenevisibleiO64668. AT.

Family and domain databases

InterProiIPR001108. Peptidase_A22A.
IPR006639. Preselin/SPP.
[Graphical view]
PANTHERiPTHR10202. PTHR10202. 1 hit.
PfamiPF01080. Presenilin. 1 hit.
[Graphical view]
PRINTSiPR01072. PRESENILIN.
SMARTiSM00730. PSN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSNA_ARATH
AccessioniPrimary (citable) accession number: O64668
Secondary accession number(s): Q8L9V0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.