O64583 (P2C28_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 28
Short name=AtPP2C28
EC=3.1.3.16

Gene names

Ordered Locus Names:	At2g34740
ORF Names:	T29F13.5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.
Sequence caution	The sequence AAC16260.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AEC09017.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O64583-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O64583-2)

The sequence of this isoform differs from the canonical sequence as follows:
     292-339: MSNDEVWDQIKKRGNAEEAAKMLIDKALARGSKDDISCVVVSFLQWID → STYECHGTLLIGAPKDRTKS

Note: May be due to an intron retention.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Probable protein phosphatase 2C 28		PRO_0000367957
Regions
	Domain	100 – 327	228	PP2C-like
	Compositional bias	52 – 74	23	Asp-rich
Sites
	Metal binding	124	1	Manganese 1 By similarity
	Metal binding	124	1	Manganese 2 By similarity
	Metal binding	125	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	286	1	Manganese 2 By similarity
	Metal binding	325	1	Manganese 2 By similarity
Natural variations
	Alternative sequence	292 – 339	48	MSNDE…LQWID → STYECHGTLLIGAPKDRTKS in isoform 2.		VSP_041311

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 13, 2012. Version 2. Checksum: 3A7310A6CBF3AC47	FASTA	339	37,881
	10 20 30 40 50 60 MKKTIKNSTH RPEDILVHAI KKRRSASGDV KNAVKLKEGE DYLRVEEEIP HDDNVVVIDD 70 80 90 100 110 120 GCDHDHDVDD NDDDEEENGR YCRREFDHGY HLVKGQMGHG MEDFIVADTK TVKGHNLGLY 130 140 150 160 170 180 AIFDGHSGSD VADYLQNHLF DNILSQPDFW RNPKKAIKRA YKSTDDYILQ NVVGPRGGST 190 200 210 220 230 240 AVTAIVIDGK KIVVANVGDS RAILCRESDV VKQITVDHEP DKERDLVKSK GGFVSQKPGN 250 260 270 280 290 300 VPRVDGQLAM TRAFGDGGLK EHISVIPNIE IAEIHDDTKF LILASDGLWK VMSNDEVWDQ 310 320 330 IKKRGNAEEA AKMLIDKALA RGSKDDISCV VVSFLQWID
	Isoform 2 [UniParc]. Checksum: E5C507B4A6E74366	FASTA	311	34,633
	10 20 30 40 50 60 MKKTIKNSTH RPEDILVHAI KKRRSASGDV KNAVKLKEGE DYLRVEEEIP HDDNVVVIDD 70 80 90 100 110 120 GCDHDHDVDD NDDDEEENGR YCRREFDHGY HLVKGQMGHG MEDFIVADTK TVKGHNLGLY 130 140 150 160 170 180 AIFDGHSGSD VADYLQNHLF DNILSQPDFW RNPKKAIKRA YKSTDDYILQ NVVGPRGGST 190 200 210 220 230 240 AVTAIVIDGK KIVVANVGDS RAILCRESDV VKQITVDHEP DKERDLVKSK GGFVSQKPGN 250 260 270 280 290 300 VPRVDGQLAM TRAFGDGGLK EHISVIPNIE IAEIHDDTKF LILASDGLWK VSTYECHGTL 310 LIGAPKDRTK S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 170-339 (ISOFORM 2). Strain: cv. Columbia.
[4]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC003096 Genomic DNA. Translation: AAC16260.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC09016.1. CP002685 Genomic DNA. Translation: AEC09017.1. Sequence problems. AK175603 mRNA. Translation: BAD43366.1.
IPI	IPI00532562. IPI00991656.
PIR	T01361.
RefSeq	NP_001189678.1. NM_001202749.1. NP_181021.4. NM_129028.4.
UniGene	At.12517. At.66356.
3D structure databases
HSSP	HSSP built from PDB template 1A6Q based on UniProtKB P35813.
ProteinModelPortal	O64583.
SMR	O64583. Positions 119-334.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	818039.
KEGG	ath:AT2G34740.
Organism-specific databases
TAIR	At2g34740.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233896.
InParanoid	O64583.
KO	K14803.
OMA	MSNDEVW.
Gene expression databases
ArrayExpress	O64583.
Genevestigator	O64583.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C28_ARATH

Accession

Primary (citable) accession number: O64583
Secondary accession number(s): F4IIV1, F4IIV2, Q681L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 13, 2012
Last modified:	May 1, 2013
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program