O64510 (PLY6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable pectate lyase 6
EC=4.2.2.2

Gene names

Ordered Locus Names:	At2g02720
ORF Names:	T20F6.14

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion. Required for its activity By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 455	430	Probable pectate lyase 6		PRO_0000024870
Sites
	Active site	327	1	Potential
	Metal binding	247	1	Calcium By similarity
	Metal binding	271	1	Calcium By similarity
	Metal binding	275	1	Calcium By similarity
Amino acid modifications
	Glycosylation	55	1	N-linked (GlcNAc...) Potential
	Glycosylation	75	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O64510 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 7C6A35A767A30CA4	FASTA	455	51,258
	10 20 30 40 50 60 MVNLGSYVFV FVALSLTVVV PSVQAHIAEY DEYWTQRQTN ALRETLESYD PNPENVTDHF 70 80 90 100 110 120 NYHAALAMET TGIVNETRRD LRQVGRGKKT TRRGGRFESL NAIDKCWRGD KNWDKNRKKL 130 140 150 160 170 180 ADCVLGFGRK TTGGKNGPIY VVTDPSDNDL LKPKPGTIRH AVTRDRPLWI IFARSMIIKL 190 200 210 220 230 240 QQELIITNDK TIDGRGAKIY ITGGAGLTLQ FVRNVIIHNI HIKQIKRGAG GLIIDSEQHF 250 260 270 280 290 300 GLRTVSDGDG INIFGATNVW IDHVSMTDCS DGMIDAIMGS TAITISNSHF TDHDEVMLFG 310 320 330 340 350 360 GTNKDVIDKK MQITVAFNHF GKRLKQRMPR VRFGLVHVVN NDYTHWEMYA IGGNMNPTII 370 380 390 400 410 420 SQGNRFIAPP IEDSKQVTKR EYTPYPEWKS WNWQSEKDYF LNGAYFVQSG KANAWSATPK 430 440 450 NPIPRKFAIR PQPGTKVRRL TKDAGTLGCK PGKSC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC002521 Genomic DNA. Translation: AAC05350.1. CP002685 Genomic DNA. Translation: AEC05615.1. BT005757 mRNA. Translation: AAO64162.1.
IPI	IPI00523896.
PIR	T00856.
RefSeq	NP_178375.1. NM_126327.3.
UniGene	At.41462. At.48411.
3D structure databases
ProteinModelPortal	O64510.
SMR	O64510. Positions 100-448.
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Proteomic databases
PRIDE	O64510.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G02720.1; AT2G02720.1; AT2G02720.
GeneID	814801.
GenomeReviews	Gene locus AT2G02720 in contig CT485783_GR.
KEGG	ath:AT2G02720.
Organism-specific databases
TAIR	At2g02720.
Phylogenomic databases
eggNOG	euNOG10883.
HOGENOM	HBG317381.
InParanoid	O64510.
OMA	DGINIFG.
PhylomeDB	O64510.
ProtClustDB	CLSN2682943.
Gene expression databases
ArrayExpress	O64510.
Genevestigator	O64510.
GermOnline	AT2G02720. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR018082. AmbAllergen. IPR007524. Pec_lyase_N. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
KO	K01728.
Pfam	PF00544. Pec_lyase_C. 1 hit. PF04431. Pec_lyase_N. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLY6_ARATH

Accession

Primary (citable) accession number: O64510

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	August 1, 1998
Last modified:	December 14, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program