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Protein

Subtilisin-like protease SBT1.2

Gene

SBT1.2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Serine protease involved in the negative regulation of stomatal density and distribution. Not active on EPFL6 (AC Q1PEY6) (PubMed:20056678). Positive regulator of water use efficiency (WUE).4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei146 – 1461Charge relay systemPROSITE-ProRule annotation
Active sitei222 – 2221Charge relay systemPROSITE-ProRule annotation
Active sitei552 – 5521Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • regulation of cell proliferation Source: TAIR
  • stomatal complex morphogenesis Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciARA:AT1G04110-MONOMER.

Protein family/group databases

MEROPSiS08.084.

Names & Taxonomyi

Protein namesi
Recommended name:
Subtilisin-like protease SBT1.21 Publication (EC:3.4.21.-Curated)
Alternative name(s):
Cucumisin-like serine protease SDD1
Protein STOMATAL DENSITY AND DISTRIBUTION 1
Subtilase subfamily 1 member 21 Publication
Short name:
AtSBT1.21 Publication
Subtilisin-like protease SDD11 Publication
Gene namesi
Name:SBT1.21 Publication
Synonyms:SDD11 Publication
Ordered Locus Names:At1g04110Imported
ORF Names:F20D22.121 Publication
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G04110.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: UniProtKB-SubCell
  • cell wall Source: GO_Central
  • external side of plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell membrane, Membrane, Secreted

Pathology & Biotechi

Disruption phenotypei

Increased stomatal density and formation of clustered stomata by enhanced stomatal development initiation and extension of stomatal cell lineages. No major impact on photosynthesis, except 30% higher CO2 assimilation rates in low-light-adapted plants exposed to high light intensities.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 775755Subtilisin-like protease SBT1.2PRO_0000405797Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi472 – 4721N-linked (GlcNAc...)Sequence analysis
Glycosylationi544 – 5441N-linked (GlcNAc...)Sequence analysis
Glycosylationi652 – 6521N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO64495.
PRIDEiO64495.

Expressioni

Tissue specificityi

Mostly expressed in leaves and cotyledons (especially in epidermal cells), and, to a lower extent, in floral buds, stems, and siliques. Strongly expressed in stomatal precursor cells (meristemoids and guard mother cells).2 Publications

Inductioni

Negatively feedback controlled by components of an SDD1-dependent signaling pathway. Repressed by GTL1.2 Publications

Gene expression databases

GenevisibleiO64495. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G04110.1.

Structurei

3D structure databases

ProteinModelPortaliO64495.
SMRiO64495. Positions 113-771.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini127 – 587461Peptidase S8Add
BLAST
Domaini388 – 47083PAAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IEU2. Eukaryota.
COG1404. LUCA.
HOGENOMiHOG000238262.
InParanoidiO64495.
OMAiPRDSHGH.
PhylomeDBiO64495.

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 3 hits.
InterProiIPR003137. PA_domain.
IPR000209. Peptidase_S8/S53_dom.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF05922. Inhibitor_I9. 1 hit.
PF02225. PA. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 2 hits.
PROSITEiPS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O64495-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPKPFFLCI IFLLFCSSSS EILQKQTYIV QLHPNSETAK TFASKFDWHL
60 70 80 90 100
SFLQEAVLGV EEEEEEPSSR LLYSYGSAIE GFAAQLTESE AEILRYSPEV
110 120 130 140 150
VAVRPDHVLQ VQTTYSYKFL GLDGFGNSGV WSKSRFGQGT IIGVLDTGVW
160 170 180 190 200
PESPSFDDTG MPSIPRKWKG ICQEGESFSS SSCNRKLIGA RFFIRGHRVA
210 220 230 240 250
NSPEESPNMP REYISARDST GHGTHTASTV GGSSVSMANV LGNGAGVARG
260 270 280 290 300
MAPGAHIAVY KVCWFNGCYS SDILAAIDVA IQDKVDVLSL SLGGFPIPLY
310 320 330 340 350
DDTIAIGTFR AMERGISVIC AAGNNGPIES SVANTAPWVS TIGAGTLDRR
360 370 380 390 400
FPAVVRLANG KLLYGESLYP GKGIKNAGRE VEVIYVTGGD KGSEFCLRGS
410 420 430 440 450
LPREEIRGKM VICDRGVNGR SEKGEAVKEA GGVAMILANT EINQEEDSID
460 470 480 490 500
VHLLPATLIG YTESVLLKAY VNATVKPKAR IIFGGTVIGR SRAPEVAQFS
510 520 530 540 550
ARGPSLANPS ILKPDMIAPG VNIIAAWPQN LGPTGLPYDS RRVNFTVMSG
560 570 580 590 600
TSMSCPHVSG ITALIRSAYP NWSPAAIKSA LMTTADLYDR QGKAIKDGNK
610 620 630 640 650
PAGVFAIGAG HVNPQKAINP GLVYNIQPVD YITYLCTLGF TRSDILAITH
660 670 680 690 700
KNVSCNGILR KNPGFSLNYP SIAVIFKRGK TTEMITRRVT NVGSPNSIYS
710 720 730 740 750
VNVKAPEGIK VIVNPKRLVF KHVDQTLSYR VWFVLKKKNR GGKVASFAQG
760 770
QLTWVNSHNL MQRVRSPISV TLKTN
Length:775
Mass (Da):83,776
Last modified:August 1, 1998 - v1
Checksum:i7740B20397C7C211
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002411 Genomic DNA. Translation: AAC16749.1.
CP002684 Genomic DNA. Translation: AEE27657.1.
PIRiT00962.
RefSeqiNP_563701.1. NM_100292.1.
UniGeneiAt.65883.

Genome annotation databases

EnsemblPlantsiAT1G04110.1; AT1G04110.1; AT1G04110.
GeneIDi839287.
GrameneiAT1G04110.1; AT1G04110.1; AT1G04110.
KEGGiath:AT1G04110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002411 Genomic DNA. Translation: AAC16749.1.
CP002684 Genomic DNA. Translation: AEE27657.1.
PIRiT00962.
RefSeqiNP_563701.1. NM_100292.1.
UniGeneiAt.65883.

3D structure databases

ProteinModelPortaliO64495.
SMRiO64495. Positions 113-771.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G04110.1.

Protein family/group databases

MEROPSiS08.084.

Proteomic databases

PaxDbiO64495.
PRIDEiO64495.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G04110.1; AT1G04110.1; AT1G04110.
GeneIDi839287.
GrameneiAT1G04110.1; AT1G04110.1; AT1G04110.
KEGGiath:AT1G04110.

Organism-specific databases

TAIRiAT1G04110.

Phylogenomic databases

eggNOGiENOG410IEU2. Eukaryota.
COG1404. LUCA.
HOGENOMiHOG000238262.
InParanoidiO64495.
OMAiPRDSHGH.
PhylomeDBiO64495.

Enzyme and pathway databases

BioCyciARA:AT1G04110-MONOMER.

Miscellaneous databases

PROiO64495.

Gene expression databases

GenevisibleiO64495. AT.

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 3 hits.
InterProiIPR003137. PA_domain.
IPR000209. Peptidase_S8/S53_dom.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF05922. Inhibitor_I9. 1 hit.
PF02225. PA. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 2 hits.
PROSITEiPS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "A subtilisin-like serine protease involved in the regulation of stomatal density and distribution in Arabidopsis thaliana."
    Berger D., Altmann T.
    Genes Dev. 14:1119-1131(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  4. "The subtilisin-like serine protease SDD1 mediates cell-to-cell signaling during Arabidopsis stomatal development."
    Von Groll U., Berger D., Altmann T.
    Plant Cell 14:1527-1539(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION, PROBABLE SUBCELLULAR LOCATION.
    Strain: cv. C24.
  5. "Photosynthetic performance of an Arabidopsis mutant with elevated stomatal density (sdd1-1) under different light regimes."
    Schlueter U., Muschak M., Berger D., Altmann T.
    J. Exp. Bot. 54:867-874(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  6. "Inferring hypotheses on functional relationships of genes: Analysis of the Arabidopsis thaliana subtilase gene family."
    Rautengarten C., Steinhauser D., Bussis D., Stintzi A., Schaller A., Kopka J., Altmann T.
    PLoS Comput. Biol. 1:E40-E40(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. "Influence of environmental factors on stomatal development."
    Casson S., Gray J.E.
    New Phytol. 178:9-23(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  8. "Cell fate transitions during stomatal development."
    Serna L.
    Bioessays 31:865-873(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  9. "Regional specification of stomatal production by the putative ligand CHALLAH."
    Abrash E.B., Bergmann D.C.
    Development 137:447-455(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: cv. Columbia.
  10. "The Arabidopsis GTL1 transcription factor regulates water use efficiency and drought tolerance by modulating stomatal density via transrepression of SDD1."
    Yoo C.Y., Pence H.E., Jin J.B., Miura K., Gosney M.J., Hasegawa P.M., Mickelbart M.V.
    Plant Cell 22:4128-4141(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY GTL1.
    Strain: cv. Columbia.

Entry informationi

Entry nameiSBT12_ARATH
AccessioniPrimary (citable) accession number: O64495
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: August 1, 1998
Last modified: February 17, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.