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Protein

Subtilisin-like protease SBT1.2

Gene

SBT1.2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Serine protease involved in the negative regulation of stomatal density and distribution. Not active on EPFL6 (AC Q1PEY6) (PubMed:20056678). Positive regulator of water use efficiency (WUE).4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei146Charge relay systemPROSITE-ProRule annotation1
Active sitei222Charge relay systemPROSITE-ProRule annotation1
Active sitei552Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

  • serine-type endopeptidase activity Source: TAIR

GO - Biological processi

  • regulation of cell proliferation Source: TAIR
  • stomatal complex morphogenesis Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciARA:AT1G04110-MONOMER.

Protein family/group databases

MEROPSiS08.084.

Names & Taxonomyi

Protein namesi
Recommended name:
Subtilisin-like protease SBT1.21 Publication (EC:3.4.21.-Curated)
Alternative name(s):
Cucumisin-like serine protease SDD1
Protein STOMATAL DENSITY AND DISTRIBUTION 1
Subtilase subfamily 1 member 21 Publication
Short name:
AtSBT1.21 Publication
Subtilisin-like protease SDD11 Publication
Gene namesi
Name:SBT1.21 Publication
Synonyms:SDD11 Publication
Ordered Locus Names:At1g04110Imported
ORF Names:F20D22.121 Publication
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G04110.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: UniProtKB-SubCell
  • cell wall Source: GO_Central
  • external side of plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell membrane, Membrane, Secreted

Pathology & Biotechi

Disruption phenotypei

Increased stomatal density and formation of clustered stomata by enhanced stomatal development initiation and extension of stomatal cell lineages. No major impact on photosynthesis, except 30% higher CO2 assimilation rates in low-light-adapted plants exposed to high light intensities.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000040579721 – 775Subtilisin-like protease SBT1.2Add BLAST755

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi472N-linked (GlcNAc...)Sequence analysis1
Glycosylationi544N-linked (GlcNAc...)Sequence analysis1
Glycosylationi652N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO64495.

Expressioni

Tissue specificityi

Mostly expressed in leaves and cotyledons (especially in epidermal cells), and, to a lower extent, in floral buds, stems, and siliques. Strongly expressed in stomatal precursor cells (meristemoids and guard mother cells).2 Publications

Inductioni

Negatively feedback controlled by components of an SDD1-dependent signaling pathway. Repressed by GTL1.2 Publications

Gene expression databases

GenevisibleiO64495. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G04110.1.

Structurei

3D structure databases

ProteinModelPortaliO64495.
SMRiO64495.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini127 – 587Peptidase S8Add BLAST461
Domaini388 – 470PAAdd BLAST83

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IEU2. Eukaryota.
COG1404. LUCA.
HOGENOMiHOG000238262.
InParanoidiO64495.
OMAiPRDSHGH.
OrthoDBiEOG09360306.
PhylomeDBiO64495.

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 3 hits.
InterProiIPR003137. PA_domain.
IPR000209. Peptidase_S8/S53_dom.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF05922. Inhibitor_I9. 1 hit.
PF02225. PA. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 2 hits.
PROSITEiPS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O64495-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPKPFFLCI IFLLFCSSSS EILQKQTYIV QLHPNSETAK TFASKFDWHL
60 70 80 90 100
SFLQEAVLGV EEEEEEPSSR LLYSYGSAIE GFAAQLTESE AEILRYSPEV
110 120 130 140 150
VAVRPDHVLQ VQTTYSYKFL GLDGFGNSGV WSKSRFGQGT IIGVLDTGVW
160 170 180 190 200
PESPSFDDTG MPSIPRKWKG ICQEGESFSS SSCNRKLIGA RFFIRGHRVA
210 220 230 240 250
NSPEESPNMP REYISARDST GHGTHTASTV GGSSVSMANV LGNGAGVARG
260 270 280 290 300
MAPGAHIAVY KVCWFNGCYS SDILAAIDVA IQDKVDVLSL SLGGFPIPLY
310 320 330 340 350
DDTIAIGTFR AMERGISVIC AAGNNGPIES SVANTAPWVS TIGAGTLDRR
360 370 380 390 400
FPAVVRLANG KLLYGESLYP GKGIKNAGRE VEVIYVTGGD KGSEFCLRGS
410 420 430 440 450
LPREEIRGKM VICDRGVNGR SEKGEAVKEA GGVAMILANT EINQEEDSID
460 470 480 490 500
VHLLPATLIG YTESVLLKAY VNATVKPKAR IIFGGTVIGR SRAPEVAQFS
510 520 530 540 550
ARGPSLANPS ILKPDMIAPG VNIIAAWPQN LGPTGLPYDS RRVNFTVMSG
560 570 580 590 600
TSMSCPHVSG ITALIRSAYP NWSPAAIKSA LMTTADLYDR QGKAIKDGNK
610 620 630 640 650
PAGVFAIGAG HVNPQKAINP GLVYNIQPVD YITYLCTLGF TRSDILAITH
660 670 680 690 700
KNVSCNGILR KNPGFSLNYP SIAVIFKRGK TTEMITRRVT NVGSPNSIYS
710 720 730 740 750
VNVKAPEGIK VIVNPKRLVF KHVDQTLSYR VWFVLKKKNR GGKVASFAQG
760 770
QLTWVNSHNL MQRVRSPISV TLKTN
Length:775
Mass (Da):83,776
Last modified:August 1, 1998 - v1
Checksum:i7740B20397C7C211
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002411 Genomic DNA. Translation: AAC16749.1.
CP002684 Genomic DNA. Translation: AEE27657.1.
PIRiT00962.
RefSeqiNP_563701.1. NM_100292.2.
UniGeneiAt.65883.

Genome annotation databases

EnsemblPlantsiAT1G04110.1; AT1G04110.1; AT1G04110.
GeneIDi839287.
GrameneiAT1G04110.1; AT1G04110.1; AT1G04110.
KEGGiath:AT1G04110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002411 Genomic DNA. Translation: AAC16749.1.
CP002684 Genomic DNA. Translation: AEE27657.1.
PIRiT00962.
RefSeqiNP_563701.1. NM_100292.2.
UniGeneiAt.65883.

3D structure databases

ProteinModelPortaliO64495.
SMRiO64495.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G04110.1.

Protein family/group databases

MEROPSiS08.084.

Proteomic databases

PaxDbiO64495.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G04110.1; AT1G04110.1; AT1G04110.
GeneIDi839287.
GrameneiAT1G04110.1; AT1G04110.1; AT1G04110.
KEGGiath:AT1G04110.

Organism-specific databases

TAIRiAT1G04110.

Phylogenomic databases

eggNOGiENOG410IEU2. Eukaryota.
COG1404. LUCA.
HOGENOMiHOG000238262.
InParanoidiO64495.
OMAiPRDSHGH.
OrthoDBiEOG09360306.
PhylomeDBiO64495.

Enzyme and pathway databases

BioCyciARA:AT1G04110-MONOMER.

Miscellaneous databases

PROiO64495.

Gene expression databases

GenevisibleiO64495. AT.

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 3 hits.
InterProiIPR003137. PA_domain.
IPR000209. Peptidase_S8/S53_dom.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF05922. Inhibitor_I9. 1 hit.
PF02225. PA. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 2 hits.
PROSITEiPS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSBT12_ARATH
AccessioniPrimary (citable) accession number: O64495
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.