O64479 (PME10_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative pectinesterase 10
Short name=PE 10
EC=3.1.1.11

Alternative name(s):
Pectin methylesterase 10
Short name=AtPME10

Gene names

Name:	PME10
Synonyms:	ARATH10
Ordered Locus Names:	At2g19150
ORF Names:	T20K24.17

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Subcellular location	Secreted › cell wall By similarity.
Tissue specificity	Expressed in siliques. Ref.4
Developmental stage	Expressed during late developmental phases of siliques. Ref.4
Sequence similarities	Belongs to the pectinesterase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Aspartyl esterase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell wall modification Inferred from electronic annotation. Source: InterPro pectin catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 339	311	Putative pectinesterase 10		PRO_0000371667
Regions
	Compositional bias	139 – 142	4	Poly-Ser
Sites
	Active site	169	1	Proton donor By similarity
	Active site	190	1	Nucleophile By similarity
	Binding site	116	1	Substrate By similarity
	Binding site	252	1	Substrate By similarity
	Binding site	254	1	Substrate By similarity
	Site	168	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	112	1	N-linked (GlcNAc...) Potential
	Glycosylation	322	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O64479 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 5A72930E4D38D912	FASTA	339	37,633
	10 20 30 40 50 60 MKGVTIHNFC YSYFKVCLLV MSLAYGSAEW DGSSSQIAKT IIVNPNDARY FKTVQSAIDS 70 80 90 100 110 120 IPLQNQDWIR ILISNGIYSE KVTIPRGKGY IYMQGGGIEK TIIAYGDHQL TNTSATFTSY 130 140 150 160 170 180 PSNIIITGIT FKNKYNIASS SSPTKPAVAA MMLGDKYAII DSSFDGFQDT LYDDYGRHYY 190 200 210 220 230 240 KRCVISGGID FIFGGAQSIF EGCTLKLRVG IYPPNEVYGT ITAQGRDSPT DKGGFVFKDC 250 260 270 280 290 300 TVMGSGKALL GRAWKSYSRV IFYRSMFSDN ILPIGWDAWK AKGQEGHITF VEFGCTGVGA 310 320 330 DTSKRVPWLT KASEKDVLQF TNLTFIDEEG WLSRLPIKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Pectin methylesterases: sequence-structural features and phylogenetic relationships." Markovic O., Janecek S. Carbohydr. Res. 339:2281-2295(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[4]	"Comprehensive expression profiling of the pectin methylesterase gene family during silique development in Arabidopsis thaliana." Louvet R., Cavel E., Gutierrez L., Guenin S., Roger D., Gillet F., Guerineau F., Pelloux J. Planta 224:782-791(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC002392 Genomic DNA. Translation: AAD12032.1. CP002685 Genomic DNA. Translation: AEC06852.1.
IPI	IPI00519186.
PIR	T00536.
RefSeq	NP_179505.1. NM_127472.1.
UniGene	At.52825.
3D structure databases
ProteinModelPortal	O64479.
SMR	O64479. Positions 46-319.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G19150.1-P.
Proteomic databases
PaxDb	O64479.
PRIDE	O64479.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G19150.1; AT2G19150.1; AT2G19150.
GeneID	816432.
KEGG	ath:AT2G19150.
Organism-specific databases
GeneFarm	459. 8.
TAIR	At2g19150.
Phylogenomic databases
eggNOG	COG4677.
HOGENOM	HOG000217409.
InParanoid	O64479.
OMA	NPNDARY.
PhylomeDB	O64479.
ProtClustDB	PLN02176.
Enzyme and pathway databases
UniPathway	UPA00545; UER00823.
Gene expression databases
Genevestigator	O64479.
Family and domain databases
Gene3D	2.160.20.10. 1 hit.
InterPro	IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. IPR000070. Pectinesterase_cat. [Graphical view]
Pfam	PF01095. Pectinesterase. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00800. PECTINESTERASE_1. False negative. PS00503. PECTINESTERASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME10_ARATH

Accession

Primary (citable) accession number: O64479

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	August 1, 1998
Last modified:	May 29, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program