Reviewed, UniProtKB/Swiss-Prot O64479 (PME10_ARATH)

Names and origin

Protein names

Recommended name:
    Putative pectinesterase 10
      Short name=PE 10
    EC=3.1.1.11
Alternative name(s):
    Pectin methylesterase 10
      Short name=AtPME10

Gene names

Name:	PME10
Synonyms:	ARATH10
Ordered Locus Names:	At2g19150
ORF Names:	T20K24.17

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Subcellular location	Secreted › cell wall By similarity.
Tissue specificity	Expressed in siliques. Ref.3
Developmental stage	Expressed during late developmental phases of siliques. Ref.3
Sequence similarities	Belongs to the pectinesterase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Aspartyl esterase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall modification Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 339	311	Putative pectinesterase 10		PRO_0000371667
Regions
	Compositional bias	139 – 142	4	Poly-Ser
Sites
	Active site	169	1	Proton donor By similarity
	Active site	190	1	Nucleophile By similarity
	Binding site	116	1	Substrate By similarity
	Binding site	252	1	Substrate By similarity
	Binding site	254	1	Substrate By similarity
	Site	168	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	112	1	N-linked (GlcNAc...) Potential
	Glycosylation	322	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O64479-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 5A72930E4D38D912	FASTA	339	37,633
	10 20 30 40 50 60 MKGVTIHNFC YSYFKVCLLV MSLAYGSAEW DGSSSQIAKT IIVNPNDARY FKTVQSAIDS 70 80 90 100 110 120 IPLQNQDWIR ILISNGIYSE KVTIPRGKGY IYMQGGGIEK TIIAYGDHQL TNTSATFTSY 130 140 150 160 170 180 PSNIIITGIT FKNKYNIASS SSPTKPAVAA MMLGDKYAII DSSFDGFQDT LYDDYGRHYY 190 200 210 220 230 240 KRCVISGGID FIFGGAQSIF EGCTLKLRVG IYPPNEVYGT ITAQGRDSPT DKGGFVFKDC 250 260 270 280 290 300 TVMGSGKALL GRAWKSYSRV IFYRSMFSDN ILPIGWDAWK AKGQEGHITF VEFGCTGVGA 310 320 330 DTSKRVPWLT KASEKDVLQF TNLTFIDEEG WLSRLPIKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Pectin methylesterases: sequence-structural features and phylogenetic relationships." Markovic O., Janecek S. Carbohydr. Res. 339:2281-2295(2004) [PubMed: 15337457] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[3]	"Comprehensive expression profiling of the pectin methylesterase gene family during silique development in Arabidopsis thaliana." Louvet R., Cavel E., Gutierrez L., Guenin S., Roger D., Gillet F., Guerineau F., Pelloux J. Planta 224:782-791(2006) [PubMed: 16622707] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC002392 Genomic DNA. Translation: AAD12032.1.
IPI	IPI00519186.
PIR	T00536.
RefSeq	NP_179505.1.
UniGene	At.52825
3D structure databases
HSSP	HSSP built from PDB template 1GQ8 based on UniProtKB P83218.
ModBase	Search...
Genome annotation databases
GeneID	816432.
GenomeReviews	Gene locus AT2G19150 in contig CT485783_GR.
KEGG	ath:AT2G19150.
NMPDR	fig|3702.1.peg.8910.
Organism-specific databases
GeneFarm	459. 8.
TAIR	At2g19150.
Phylogenomic databases
OMA	WIRILIS.
Gene expression databases
ArrayExpress	O64479.
Genevestigator	O64479.
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR018040. Pectinesterase_AS. IPR000070. Pectinesterase_cat. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF01095. Pectinesterase. 2 hits. [Graphical view]
PROSITE	PS00800. PECTINESTERASE_1. False negative. PS00503. PECTINESTERASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME10_ARATH

Accession

Primary (citable) accession number: O64479

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)