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Protein

Putative cadmium/zinc-transporting ATPase HMA4

Gene

HMA4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in cadmium/zinc transport.Curated

Catalytic activityi

ATP + H2O + Cd2+(In) = ADP + phosphate + Cd2+(Out).
ATP + H2O + Zn2+(In) = ADP + phosphate + Zn2+(Out).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4014-aspartylphosphate intermediateBy similarity1
Metal bindingi601MagnesiumPROSITE-ProRule annotation1
Metal bindingi605MagnesiumPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • cadmium ion transport Source: TAIR
  • metal ion transport Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to cobalt ion Source: TAIR
  • response to metal ion Source: TAIR
  • response to zinc ion Source: TAIR
  • zinc II ion transport Source: TAIR
  • zinc ion homeostasis Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Cadmium, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT2G19110-MONOMER.

Protein family/group databases

TCDBi3.A.3.6.18. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative cadmium/zinc-transporting ATPase HMA4 (EC:3.6.3.3, EC:3.6.3.5)
Alternative name(s):
Protein HEAVY METAL ATPASE 4
Putative cadmium/zinc-transporting ATPase 2
Gene namesi
Name:HMA4
Ordered Locus Names:At2g19110
ORF Names:T20K24.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G19110.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 93CytoplasmicSequence analysisAdd BLAST93
Transmembranei94 – 115HelicalSequence analysisAdd BLAST22
Topological domaini116 – 118ExtracellularSequence analysis3
Transmembranei119 – 138HelicalSequence analysisAdd BLAST20
Topological domaini139 – 145CytoplasmicSequence analysis7
Transmembranei146 – 166HelicalSequence analysisAdd BLAST21
Topological domaini167ExtracellularSequence analysis1
Transmembranei168 – 188HelicalSequence analysisAdd BLAST21
Topological domaini189 – 314CytoplasmicSequence analysisAdd BLAST126
Transmembranei315 – 337HelicalSequence analysisAdd BLAST23
Topological domaini338 – 345ExtracellularSequence analysis8
Transmembranei346 – 363HelicalSequence analysisAdd BLAST18
Topological domaini364 – 656CytoplasmicSequence analysisAdd BLAST293
Transmembranei657 – 676HelicalSequence analysisAdd BLAST20
Topological domaini677 – 680ExtracellularSequence analysis4
Transmembranei681 – 700HelicalSequence analysisAdd BLAST20
Topological domaini701 – 1172CytoplasmicSequence analysisAdd BLAST472

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • intracellular membrane-bounded organelle Source: GO_Central
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000463991 – 1172Putative cadmium/zinc-transporting ATPase HMA4Add BLAST1172

Proteomic databases

PaxDbiO64474.

Expressioni

Gene expression databases

ExpressionAtlasiO64474. baseline and differential.
GenevisibleiO64474. AT.

Interactioni

Protein-protein interaction databases

BioGridi1785. 1 interactor.
IntActiO64474. 1 interactor.
STRINGi3702.AT2G19110.1.

Structurei

Secondary structure

11172
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 8Combined sources3
Beta strandi16 – 23Combined sources8
Turni28 – 31Combined sources4
Helixi32 – 41Combined sources10
Beta strandi42 – 51Combined sources10
Turni52 – 55Combined sources4
Beta strandi56 – 61Combined sources6
Turni63 – 65Combined sources3
Helixi68 – 78Combined sources11
Beta strandi82 – 85Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KKHNMR-A2-96[»]
ProteinModelPortaliO64474.
SMRiO64474.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO64474.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 84HMAPROSITE-ProRule annotationAdd BLAST67

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1157 – 1169Poly-HisAdd BLAST13

Sequence similaritiesi

Contains 1 HMA domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0207. Eukaryota.
COG2217. LUCA.
HOGENOMiHOG000250399.
InParanoidiO64474.
KOiK01534.
OMAiKDEHCES.
OrthoDBiEOG09360170.
PhylomeDBiO64474.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O64474-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALQNKEEEK KKVKKLQKSY FDVLGICCTS EVPIIENILK SLDGVKEYSV
60 70 80 90 100
IVPSRTVIVV HDSLLISPFQ IAKALNEARL EANVRVNGET SFKNKWPSPF
110 120 130 140 150
AVVSGLLLLL SFLKFVYSPL RWLAVAAVAA GIYPILAKAF ASIKRPRIDI
160 170 180 190 200
NILVIITVIA TLAMQDFMEA AAVVFLFTIS DWLETRASYK ATSVMQSLMS
210 220 230 240 250
LAPQKAIIAE TGEEVEVDEV KVDTVVAVKA GETIPIDGIV VDGNCEVDEK
260 270 280 290 300
TLTGEAFPVP KQRDSTVWAG TINLNGYICV KTTSLAGDCV VAKMAKLVEE
310 320 330 340 350
AQSSKTKSQR LIDKCSQYYT PAIILVSACV AIVPVIMKVH NLKHWFHLAL
360 370 380 390 400
VVLVSGCPCG LILSTPVATF CALTKAATSG LLIKSADYLD TLSKIKIVAF
410 420 430 440 450
DKTGTITRGE FIVIDFKSLS RDINLRSLLY WVSSVESKSS HPMAATIVDY
460 470 480 490 500
AKSVSVEPRP EEVEDYQNFP GEGIYGKIDG NDIFIGNKKI ASRAGCSTVP
510 520 530 540 550
EIEVDTKGGK TVGYVYVGER LAGFFNLSDA CRSGVSQAMA ELKSLGIKTA
560 570 580 590 600
MLTGDNQAAA MHAQEQLGNV LDVVHGDLLP EDKSRIIQEF KKEGPTAMVG
610 620 630 640 650
DGVNDAPALA TADIGISMGI SGSALATQTG NIILMSNDIR RIPQAVKLAR
660 670 680 690 700
RARRKVVENV CLSIILKAGI LALAFAGHPL IWAAVLVDVG TCLLVIFNSM
710 720 730 740 750
LLLREKKKIG NKKCYRASTS KLNGRKLEGD DDYVVDLEAG LLTKSGNGQC
760 770 780 790 800
KSSCCGDKKN QENVVMMKPS SKTSSDHSHP GCCGDKKEEK VKPLVKDGCC
810 820 830 840 850
SEKTRKSEGD MVSLSSCKKS SHVKHDLKMK GGSGCCASKN EKGKEVVAKS
860 870 880 890 900
CCEKPKQQVE SVGDCKSGHC EKKKQAEDIV VPVQIIGHAL THVEIELQTK
910 920 930 940 950
ETCKTSCCDS KEKVKETGLL LSSENTPYLE KGVLIKDEGN CKSGSENMGT
960 970 980 990 1000
VKQSCHEKGC SDEKQTGEIT LASEEETDDQ DCSSGCCVNE GTVKQSFDEK
1010 1020 1030 1040 1050
KHSVLVEKEG LDMETGFCCD AKLVCCGNTE GEVKEQCRLE IKKEEHCKSG
1060 1070 1080 1090 1100
CCGEEIQTGE ITLVSEEETE STNCSTGCCV DKEEVTQTCH EKPASLVVSG
1110 1120 1130 1140 1150
LEVKKDEHCE SSHRAVKVET CCKVKIPEAC ASKCRDRAKR HSGKSCCRSY
1160 1170
AKELCSHRHH HHHHHHHHHV SA
Length:1,172
Mass (Da):127,209
Last modified:May 1, 1999 - v2
Checksum:i1E913B9450443440
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ297264 mRNA. Translation: CAC19544.1.
AC002392 Genomic DNA. Translation: AAD12041.1.
CP002685 Genomic DNA. Translation: AEC06848.1.
AY096796 mRNA. Translation: AAM19954.1.
PIRiF84572.
RefSeqiNP_179501.1. NM_127468.5.
UniGeneiAt.25023.

Genome annotation databases

EnsemblPlantsiAT2G19110.1; AT2G19110.1; AT2G19110.
GeneIDi816428.
GrameneiAT2G19110.1; AT2G19110.1; AT2G19110.
KEGGiath:AT2G19110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ297264 mRNA. Translation: CAC19544.1.
AC002392 Genomic DNA. Translation: AAD12041.1.
CP002685 Genomic DNA. Translation: AEC06848.1.
AY096796 mRNA. Translation: AAM19954.1.
PIRiF84572.
RefSeqiNP_179501.1. NM_127468.5.
UniGeneiAt.25023.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KKHNMR-A2-96[»]
ProteinModelPortaliO64474.
SMRiO64474.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi1785. 1 interactor.
IntActiO64474. 1 interactor.
STRINGi3702.AT2G19110.1.

Protein family/group databases

TCDBi3.A.3.6.18. the p-type atpase (p-atpase) superfamily.

Proteomic databases

PaxDbiO64474.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G19110.1; AT2G19110.1; AT2G19110.
GeneIDi816428.
GrameneiAT2G19110.1; AT2G19110.1; AT2G19110.
KEGGiath:AT2G19110.

Organism-specific databases

TAIRiAT2G19110.

Phylogenomic databases

eggNOGiKOG0207. Eukaryota.
COG2217. LUCA.
HOGENOMiHOG000250399.
InParanoidiO64474.
KOiK01534.
OMAiKDEHCES.
OrthoDBiEOG09360170.
PhylomeDBiO64474.

Enzyme and pathway databases

BioCyciARA:AT2G19110-MONOMER.

Miscellaneous databases

EvolutionaryTraceiO64474.
PROiO64474.

Gene expression databases

ExpressionAtlasiO64474. baseline and differential.
GenevisibleiO64474. AT.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHMA4_ARATH
AccessioniPrimary (citable) accession number: O64474
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.