Putative cadmium/zinc-transporting ATPase 2 - Arabidopsis thaliana (Mouse-ear cress)

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O64474 (AHM2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Putative cadmium/zinc-transporting ATPase 2
EC=3.6.3.3
EC=3.6.3.5

Gene names

Name:	HMA4
Ordered Locus Names:	At2g19110
ORF Names:	T20K24.12

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1172 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in cadmium/zinc transport Potential.
Catalytic activity	ATP + H₂O + Cd²⁺(In) = ADP + phosphate + Cd²⁺(Out). ATP + H₂O + Zn²⁺(In) = ADP + phosphate + Zn²⁺(Out).
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. [View classification] Contains 1 HMA domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Cadmium Magnesium Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro response to cadmium ion Inferred from mutant phenotype. Source: TAIR response to cobalt ion Inferred from mutant phenotype. Source: TAIR response to zinc ion Inferred from mutant phenotype. Source: TAIR zinc ion homeostasis Inferred from genetic interaction. Source: TAIR
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: TAIR plasmodesma Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cadmium-exporting ATPase activity Inferred from electronic annotation. Source: EC copper-exporting ATPase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: TAIR zinc-exporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1172

1172

Putative cadmium/zinc-transporting ATPase 2

PRO_0000046399

Regions

Topological domain

1 – 93

Cytoplasmic Potential

Transmembrane

94 – 115

Helical; Potential

Topological domain

116 – 118

Extracellular Potential

Transmembrane

119 – 138

Helical; Potential

Topological domain

139 – 145

Cytoplasmic Potential

Transmembrane

146 – 166

Helical; Potential

Topological domain

167

Extracellular Potential

Transmembrane

168 – 188

Helical; Potential

Topological domain

189 – 314

126

Cytoplasmic Potential

Transmembrane

315 – 337

Helical; Potential

Topological domain

338 – 345

Extracellular Potential

Transmembrane

346 – 363

Helical; Potential

Topological domain

364 – 656

293

Cytoplasmic Potential

Transmembrane

657 – 676

Helical; Potential

Topological domain

677 – 680

Extracellular Potential

Transmembrane

681 – 700

Helical; Potential

Topological domain

701 – 1172

472

Cytoplasmic Potential

Domain

18 – 84

HMA

Compositional bias

1157 – 1169

Poly-His

Sites

Active site

401

4-aspartylphosphate intermediate By similarity

Metal binding

601

Magnesium By similarity

Metal binding

605

Magnesium By similarity

Secondary structure

1172

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

O64474 [UniParc].

Last modified May 1, 1999. Version 2.
Checksum: 1E913B9450443440
Show »

FASTA

1,172

127,209

        10         20         30         40         50         60 
MALQNKEEEK KKVKKLQKSY FDVLGICCTS EVPIIENILK SLDGVKEYSV IVPSRTVIVV 

        70         80         90        100        110        120 
HDSLLISPFQ IAKALNEARL EANVRVNGET SFKNKWPSPF AVVSGLLLLL SFLKFVYSPL 

       130        140        150        160        170        180 
RWLAVAAVAA GIYPILAKAF ASIKRPRIDI NILVIITVIA TLAMQDFMEA AAVVFLFTIS 

       190        200        210        220        230        240 
DWLETRASYK ATSVMQSLMS LAPQKAIIAE TGEEVEVDEV KVDTVVAVKA GETIPIDGIV 

       250        260        270        280        290        300 
VDGNCEVDEK TLTGEAFPVP KQRDSTVWAG TINLNGYICV KTTSLAGDCV VAKMAKLVEE 

       310        320        330        340        350        360 
AQSSKTKSQR LIDKCSQYYT PAIILVSACV AIVPVIMKVH NLKHWFHLAL VVLVSGCPCG 

       370        380        390        400        410        420 
LILSTPVATF CALTKAATSG LLIKSADYLD TLSKIKIVAF DKTGTITRGE FIVIDFKSLS 

       430        440        450        460        470        480 
RDINLRSLLY WVSSVESKSS HPMAATIVDY AKSVSVEPRP EEVEDYQNFP GEGIYGKIDG 

       490        500        510        520        530        540 
NDIFIGNKKI ASRAGCSTVP EIEVDTKGGK TVGYVYVGER LAGFFNLSDA CRSGVSQAMA 

       550        560        570        580        590        600 
ELKSLGIKTA MLTGDNQAAA MHAQEQLGNV LDVVHGDLLP EDKSRIIQEF KKEGPTAMVG 

       610        620        630        640        650        660 
DGVNDAPALA TADIGISMGI SGSALATQTG NIILMSNDIR RIPQAVKLAR RARRKVVENV 

       670        680        690        700        710        720 
CLSIILKAGI LALAFAGHPL IWAAVLVDVG TCLLVIFNSM LLLREKKKIG NKKCYRASTS 

       730        740        750        760        770        780 
KLNGRKLEGD DDYVVDLEAG LLTKSGNGQC KSSCCGDKKN QENVVMMKPS SKTSSDHSHP 

       790        800        810        820        830        840 
GCCGDKKEEK VKPLVKDGCC SEKTRKSEGD MVSLSSCKKS SHVKHDLKMK GGSGCCASKN 

       850        860        870        880        890        900 
EKGKEVVAKS CCEKPKQQVE SVGDCKSGHC EKKKQAEDIV VPVQIIGHAL THVEIELQTK 

       910        920        930        940        950        960 
ETCKTSCCDS KEKVKETGLL LSSENTPYLE KGVLIKDEGN CKSGSENMGT VKQSCHEKGC 

       970        980        990       1000       1010       1020 
SDEKQTGEIT LASEEETDDQ DCSSGCCVNE GTVKQSFDEK KHSVLVEKEG LDMETGFCCD 

      1030       1040       1050       1060       1070       1080 
AKLVCCGNTE GEVKEQCRLE IKKEEHCKSG CCGEEIQTGE ITLVSEEETE STNCSTGCCV 

      1090       1100       1110       1120       1130       1140 
DKEEVTQTCH EKPASLVVSG LEVKKDEHCE SSHRAVKVET CCKVKIPEAC ASKCRDRAKR 

      1150       1160       1170 
HSGKSCCRSY AKELCSHRHH HHHHHHHHHV SA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Functional expression of AtHMA4, a P1B-type ATPase of the Zn/Co/Cd/Pb subclass." Mills R.F., Krijger G.C., Baccarini P.J., Hall J.L., Williams L.E. Plant J. 35:164-176(2003) [PubMed: 12848823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Root.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ297264 mRNA. Translation: CAC19544.1.
AC002392 Genomic DNA. Translation: AAD12041.1.
CP002685 Genomic DNA. Translation: AEC06848.1.
AY096796 mRNA. Translation: AAM19954.1.

IPI

IPI00531678.

PIR

F84572.

RefSeq

NP_179501.1. NM_127468.4.

UniGene

At.25023.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KKH	NMR	-	A	2-96	[»]

ProteinModelPortal

O64474.

SMR

O64474. Positions 2-96, 193-302, 377-656.

ModBase

Search...

Protein-protein interaction databases

IntAct

O64474. 1 interaction.

STRING

O64474.

Proteomic databases

PRIDE

O64474.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT2G19110.1; AT2G19110.1; AT2G19110.

GeneID

816428.

GenomeReviews

Gene locus AT2G19110 in contig CT485783_GR.

KEGG

ath:AT2G19110.

Organism-specific databases

TAIR

At2g19110.

Phylogenomic databases

eggNOG

KOG0207.

HOGENOM

HBG507745.

InParanoid

O64474.

OMA

DCSSGCC.

PhylomeDB

O64474.

ProtClustDB

CLSN2683157.

Gene expression databases

ArrayExpress

O64474.

Genevestigator

O64474.

GermOnline

AT2G19110. Arabidopsis thaliana.

Family and domain databases

InterPro

IPR008250. ATPase_P-typ_ATPase-assoc-dom.
IPR006403. ATPase_P-typ_cat/Cu-transptr.
IPR006404. ATPase_P-typ_Cd/Co/Hg/Pb/Zn.
IPR023300. ATPase_P-typ_cyto_domA.
IPR023299. ATPase_P-typ_cyto_domN.
IPR006416. ATPase_P-typ_heavy-metal.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR005834. Dehalogen-like_hydro.
IPR023214. HAD-like_dom.
IPR006121. HeavyMe-assoc_HMA.
[Graphical view]

Gene3D

G3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 1 hit.
G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit.
G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.

K01534.

Pfam

PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]

PRINTS

PR00119. CATATPASE.
PR00943. CUATPASE.

SUPFAM

SSF56784. HAD-like_dom. 1 hit.
SSF55008. HeavyMe_transpt. 1 hit.

TIGRFAMs

TIGR01512. ATPase-IB2_Cd. 1 hit.
TIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.

PROSITE

PS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. False negative.
PS50846. HMA_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

AHM2_ARATH

Accession

Primary (citable) accession number: O64474

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 1, 1999
Last modified:	November 16, 2011
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents