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O64464

- PSB1_ORYSJ

UniProt

O64464 - PSB1_ORYSJ

Protein

Proteasome subunit beta type-1

Gene

PBF1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. proteolysis involved in cellular protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Enzyme and pathway databases

    ReactomeiREACT_205610. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_205939. Autodegradation of the E3 ubiquitin ligase COP1.
    REACT_207219. APC/C:Cdc20 mediated degradation of Securin.
    REACT_212204. Separation of Sister Chromatids.
    REACT_214064. ER-Phagosome pathway.
    REACT_219834. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_223172. Antigen processing: Ubiquitination & Proteasome degradation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit beta type-1 (EC:3.4.25.1)
    Alternative name(s):
    20S proteasome alpha subunit F
    20S proteasome subunit beta-6
    Gene namesi
    Name:PBF1
    Ordered Locus Names:Os09g0505600, LOC_Os09g32800
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 9

    Organism-specific databases

    GrameneiO64464.

    Subcellular locationi

    Cytoplasm PROSITE-ProRule annotation. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. proteasome core complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 221221Proteasome subunit beta type-1PRO_0000148039Add
    BLAST

    Proteomic databases

    PaxDbiO64464.
    PRIDEiO64464.

    Interactioni

    Subunit structurei

    The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.By similarity

    Protein-protein interaction databases

    STRINGi39947.LOC_Os09g32800.1.

    Structurei

    3D structure databases

    ProteinModelPortaliO64464.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1B family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    HOGENOMiHOG000091081.
    KOiK02732.
    OMAiQCRAGGA.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O64464-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRRGDWVYE NNGGTCVAIA GADYCVVAAD TRLSVGYNIL TRDHSKICEL    50
    ADKCALASSG FQGDIKALHK NLAARELLYQ HQHNKRMSCP AMAQLLSNTL 100
    YYKRFFPYYA FNVLGGLDSE GKGCVFTYDA VGSYERTGYS AQGTGSALIM 150
    PVLDNQLKSP SPLLLPARDA VTPLSETEAV DLVKDVFASA TERDIYTGDK 200
    LEIVVINKAG TKREYIDLRK D 221
    Length:221
    Mass (Da):24,282
    Last modified:August 1, 1998 - v1
    Checksum:iF040EE0C2CEA5C64
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB014058 mRNA. Translation: BAA28276.1.
    AC108753 Genomic DNA. No translation available.
    RefSeqiNP_001063603.1. NM_001070138.1.
    UniGeneiOs.3736.

    Genome annotation databases

    EnsemblPlantsiOS09T0505600-01; OS09T0505600-01; OS09G0505600.
    GeneIDi4347507.
    KEGGiosa:4347507.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB014058 mRNA. Translation: BAA28276.1 .
    AC108753 Genomic DNA. No translation available.
    RefSeqi NP_001063603.1. NM_001070138.1.
    UniGenei Os.3736.

    3D structure databases

    ProteinModelPortali O64464.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 39947.LOC_Os09g32800.1.

    Proteomic databases

    PaxDbi O64464.
    PRIDEi O64464.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS09T0505600-01 ; OS09T0505600-01 ; OS09G0505600 .
    GeneIDi 4347507.
    KEGGi osa:4347507.

    Organism-specific databases

    Gramenei O64464.

    Phylogenomic databases

    eggNOGi COG0638.
    HOGENOMi HOG000091081.
    KOi K02732.
    OMAi QCRAGGA.

    Enzyme and pathway databases

    Reactomei REACT_205610. Cross-presentation of soluble exogenous antigens (endosomes).
    REACT_205939. Autodegradation of the E3 ubiquitin ligase COP1.
    REACT_207219. APC/C:Cdc20 mediated degradation of Securin.
    REACT_212204. Separation of Sister Chromatids.
    REACT_214064. ER-Phagosome pathway.
    REACT_219834. AUF1 (hnRNP D0) destabilizes mRNA.
    REACT_223172. Antigen processing: Ubiquitination & Proteasome degradation.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR016050. Proteasome_bsu_CS.
    IPR001353. Proteasome_sua/b.
    IPR023333. Proteasome_suB-type.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS00854. PROTEASOME_BETA_1. 1 hit.
    PS51476. PROTEASOME_BETA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structural features of the 20S proteasome subunits of rice (Oryza sativa)."
      Sassa H., Oguchi S., Inoue T., Hirano H.
      Gene 250:61-66(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Nipponbare.
      Tissue: Root.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.

    Entry informationi

    Entry nameiPSB1_ORYSJ
    AccessioniPrimary (citable) accession number: O64464
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 18, 2001
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3