Reviewed, UniProtKB/Swiss-Prot O64422 (F16P1_ORYSJ)

Names and origin

Protein names

Recommended name:
    Fructose-1,6-bisphosphatase, chloroplastic
      Short name=FBPase
    EC=3.1.3.11
Alternative name(s):
    D-fructose-1,6-bisphosphate 1-phosphohydrolase

Gene names

Ordered Locus Names:	Os03g0267300, LOC_Os03g16050
ORF Names:	OJ1364E02.13, OJA1364E02.2

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Homotetramer By similarity.
Subcellular location	Plastid › chloroplast stroma.
Miscellaneous	In plants there are two FBPase isozymes: one in the cytosol and the other in the chloroplast.
Sequence similarities	Belongs to the FBPase class 1 family.

Ontologies

Keywords
Biological process	Calvin cycle Carbohydrate metabolism
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 47	47	Chloroplast Potential
	Chain	48 – 406	359	Fructose-1,6-bisphosphatase, chloroplastic		PRO_0000008816
Regions
	Region	181 – 184	4	Substrate binding By similarity
Sites
	Metal binding	128	1	Magnesium 1 By similarity
	Metal binding	157	1	Magnesium 1 By similarity
	Metal binding	157	1	Magnesium 2 By similarity
	Metal binding	178	1	Magnesium 2 By similarity
	Metal binding	178	1	Magnesium 3 By similarity
	Metal binding	180	1	Magnesium 2; via carbonyl oxygen By similarity
	Metal binding	181	1	Magnesium 3 By similarity
	Metal binding	354	1	Magnesium 3 By similarity
	Binding site	286	1	Substrate By similarity
	Binding site	318	1	Substrate By similarity
	Binding site	336	1	Substrate By similarity
	Binding site	338	1	Substrate By similarity
	Binding site	348	1	Substrate By similarity
Amino acid modifications
	Disulfide bond	222 ↔ 227		Redox-active (light-modulated) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O64422-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 1665BB680EC06069	FASTA	406	43,604
	10 20 30 40 50 60 MAAAATTSSH LLLLSRQQAA ASLQCGLSFR RQPGRLAGGS SAPSVRCMAA VDTASAPAAT 70 80 90 100 110 120 EASKKSSYEI TTLTTWLLKQ EQAGTIDGEM TIVLASISTA CKQIASLVQR APISNLTGVQ 130 140 150 160 170 180 GAVNVQGEDQ KKLDVVSNEV FSNCLKSSGR TGVIASEEED VPVAVEESYS GNYIVVFDPL 190 200 210 220 230 240 DGSSNIDAAV STGSIFGIYS PNDECLADIA DDQNLDQVEQ RCIVSVCQPG SNLLAAGYCM 250 260 270 280 290 300 YSSSVIFVLT IGTGVYVFTL DPMYGEFVLT QEKVQIPKAG KIYAFNEGNY ALWDDKLKSY 310 320 330 340 350 360 MDSLKEPGPS GKPYSARYIG SLVGDFHRTL LYGGIYGYPR DQKSKNGKLR LLYECAPMSF 370 380 390 400 IVEQAGGKGS DGHQRILDIM PTEIHQRVPL YIGSVEEVEK VEKFLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a cDNA encoding plastidic fructose-1,6-Bisphosphatase from rice (Oryza sativa L.)." Takahashi S., Hirose T., Imaizumi N., Ohsugi R. Plant Gene Register PGR98-083 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare. Tissue: Leaf.
[2]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB007194 mRNA. Translation: BAA25423.1. AC135208 Genomic DNA. Translation: AAP06885.1. AC139168 Genomic DNA. Translation: AAP06892.1.
RefSeq	NP_001049664.1.
UniGene	Os.11756
3D structure databases
HSSP	HSSP built from PDB template 1DBZ based on UniProtKB P46275.
SMR	O64422. Positions 68-406.
ModBase	Search...
Genome annotation databases
GeneID	4332364.
KEGG	osa:4332364.
Organism-specific databases
Gramene	O64422.
Family and domain databases
InterPro	IPR000146. Fructose_bisphosphatase. IPR020548. Fructose_bisphosphatase_AS. [Graphical view]
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PRINTS	PR00115. F16BPHPHTASE.
ProDom	PD001491. In_FB_phphtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00124. FBPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F16P1_ORYSJ

Accession

Primary (citable) accession number: O64422
Secondary accession number(s): Q7G5R1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	September 22, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)