Reviewed, UniProtKB/Swiss-Prot O64407 (AMYB_VIGUN)

Names and origin

Protein names

Recommended name:
    Beta-amylase
    EC=3.2.1.2
Alternative name(s):
    1,4-alpha-D-glucan maltohydrolase

Gene names

Name:

BMY1

Organism

Vigna unguiculata (Cowpea)

Taxonomic identifier

3917 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Vigna

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.
Sequence similarities	Belongs to the glycosyl hydrolase 14 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-amylase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Beta-amylase		PRO_0000153940
Sites
	Active site	187	1	By similarity
	Active site	381	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O64407-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: F2EDDBE1E307C1A5	FASTA	496	56,237
	10 20 30 40 50 60 MASLDKNMLL NYVPVYVMLP LGVVSVNNVF EDPEGLKEQL VQLREAGVDG VMVDVWWGII 70 80 90 100 110 120 EQKGPKQYDW SAYKSLFQLV QECGLKLQAI MSFHQCGGNV GDVVNIPIPQ WVLDIGESDP 130 140 150 160 170 180 DIFYTNRSGT RDKEYLTIGV DNKPIFHGRT AIEVYSDYMK SFRENMSDFL KSEVIIDIEV 190 200 210 220 230 240 GLGPAGELRY PSYPQNQGWV FPGIGEFQCY DKYLKAEFKA AAARAGHSEW ELPDDAGTYN 250 260 270 280 290 300 DVPESTEFFK TNGTYLTEKG KFFLTWYSNQ LLNHGDEILD EANKAFLGCK VNLAIKVSGI 310 320 330 340 350 360 HWWYKAQNHA AELTAGYYNL DDRDGYRPIA KMVSRHHASL NFTCLEMRDS EQSSDAQSGP 370 380 390 400 410 420 QELVQQVLSG GWRENIEVAG ENALSRYDAT AYNQIILNAR PQGVNKDGPP KHRMYGVTYL 430 440 450 460 470 480 RLSDELLQQS NFDIFKKFVV KMHADQDYCE DPQEYNHGIP PLKRSEPKIP VDVLNEATKP 490 IPPFPWDSET DMKVDG

References

[1]	Bhullar S.S., Willmitzer L., Kossmann J. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. C-88.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ225087 mRNA. Translation: CAA12395.1.
PIR	T11575.
3D structure databases
SMR	O64407. Positions 7-496.
ModBase	Search...
Protein family/group databases
CAZy	GH14. Glycoside Hydrolase Family 14.
Enzyme and pathway databases
BRENDA	3.2.1.2. 2456.
Family and domain databases
InterPro	IPR001554. Glyco_hydro_14. IPR018238. Glyco_hydro_14_CS. IPR001371. Glyco_hydro_14B_pln. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF01373. Glyco_hydro_14. 1 hit. [Graphical view]
PRINTS	PR00750. BETAAMYLASE. PR00842. GLHYDLASE14B.
PROSITE	PS00506. BETA_AMYLASE_1. 1 hit. PS00679. BETA_AMYLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMYB_VIGUN

Accession

Primary (citable) accession number: O64407

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)