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Protein

Wheatwin-1

Gene

PR4A

Organism
Triticum aestivum (Wheat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Shows antifungal activity towards B.cinerea and towards the wheat-specific pathogenic fungi F.culmorum and F.graminearum (groups 1 and 2). Has ribonuclease activity.2 Publications

Enzyme regulationi

Inhibited by 5'-ADP.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Fungicide, Hydrolase, Nuclease, Pathogenesis-related protein

Keywords - Biological processi

Hypersensitive response, Plant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Wheatwin-1
Alternative name(s):
Pathogenesis-related protein 4a
Protein 0.14
RNase (EC:3.1.-.-)
Gene namesi
Name:PR4A
OrganismiTriticum aestivum (Wheat)
Taxonomic identifieri4565 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeTriticinaeTriticum
Proteomesi
  • UP000019116 Componenti: Unassembled WGS sequence

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi32 – 321H → G: Loss of antifungal activity. Reduces ribonuclease activity 60%. 1 Publication
Mutagenesisi32 – 321H → L: Loss of antifungal activity. Reduces ribonuclease activity 62%. Reduces ribonuclease activity 92%; when asociated with L-134. 1 Publication
Mutagenesisi134 – 1341H → L: Loss of antifungal activity. Reduces ribonuclease activity 92%; when asociated with L-32. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21211 PublicationAdd
BLAST
Chaini22 – 146125Wheatwin-1PRO_0000002793Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei22 – 221Pyrrolidone carboxylic acid
Disulfide bondi52 ↔ 84By similarity
Disulfide bondi73 ↔ 107By similarity
Disulfide bondi87 ↔ 144By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Expressioni

Inductioni

Up-regulated by fungal infection, salicylic acid (SA), benzo (1,2,3) thiodiazole-7-carbothioic acid S-methyl ester (BTH), methyl jasmonate (MeJA) and wounding.1 Publication

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1C2Zmodel-A22-146[»]
ProteinModelPortaliO64392.
SMRiO64392. Positions 22-146.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 146125BarwinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 barwin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.40.40.10. 1 hit.
InterProiIPR018226. Barwin_CS.
IPR001153. Barwin_dom.
IPR009009. RlpA-like_DPBB.
[Graphical view]
PfamiPF00967. Barwin. 1 hit.
[Graphical view]
PRINTSiPR00602. BARWIN.
ProDomiPD004535. Barwin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50685. SSF50685. 1 hit.
PROSITEiPS00771. BARWIN_1. 1 hit.
PS00772. BARWIN_2. 1 hit.
PS51174. BARWIN_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O64392-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAARPMLVVA LLCAAAAAAT AQQATNVRAT YHYYRPAQNN WDLGAPAVSA
60 70 80 90 100
YCATWDASKP LSWRSKYGWT AFCGPAGAHG QASCGKCLQV TNPATGAQIT
110 120 130 140
ARIVDQCANG GLDLDWDTVF TKIDTNGIGY QQGHLNVNYQ FVDCRD
Length:146
Mass (Da):15,635
Last modified:August 1, 1998 - v1
Checksum:iF23B79E93CC2D7AA
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti109 – 1091N → D.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ006098 Genomic DNA. Translation: CAA06856.1.
PIRiA53882.
T06485.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ006098 Genomic DNA. Translation: CAA06856.1.
PIRiA53882.
T06485.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1C2Zmodel-A22-146[»]
ProteinModelPortaliO64392.
SMRiO64392. Positions 22-146.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.40.40.10. 1 hit.
InterProiIPR018226. Barwin_CS.
IPR001153. Barwin_dom.
IPR009009. RlpA-like_DPBB.
[Graphical view]
PfamiPF00967. Barwin. 1 hit.
[Graphical view]
PRINTSiPR00602. BARWIN.
ProDomiPD004535. Barwin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50685. SSF50685. 1 hit.
PROSITEiPS00771. BARWIN_1. 1 hit.
PS00772. BARWIN_2. 1 hit.
PS51174. BARWIN_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWHW1_WHEAT
AccessioniPrimary (citable) accession number: O64392
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: August 1, 1998
Last modified: February 17, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.