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O64174

- BNRDF_BPSPB

UniProt

O64174 - BNRDF_BPSPB

Protein

Ribonucleoside-diphosphate reductase subunit beta

Gene

bnrdF

Organism
Bacillus phage SPbeta (Bacillus phage SPBc2) (Bacteriophage SP-beta)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi66 – 661Iron 1PROSITE-ProRule annotation
    Metal bindingi97 – 971Iron 1PROSITE-ProRule annotation
    Metal bindingi97 – 971Iron 2By similarity
    Metal bindingi101 – 1011Iron 1PROSITE-ProRule annotation
    Active sitei105 – 1051PROSITE-ProRule annotation
    Metal bindingi164 – 1641Iron 2By similarity
    Metal bindingi198 – 1981Iron 2By similarity
    Metal bindingi201 – 2011Iron 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
    2. deoxyribonucleotide biosynthetic process Source: InterPro
    3. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase subunit beta (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase small subunit
    Gene namesi
    Name:bnrdF
    Synonyms:yosP
    OrganismiBacillus phage SPbeta (Bacillus phage SPBc2) (Bacteriophage SP-beta)
    Taxonomic identifieri66797 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeSpbetalikevirus
    Virus hostiBacillus pumilus (Bacillus mesentericus) [TaxID: 1408]
    Bacillus subtilis [TaxID: 1423]
    ProteomesiUP000009091: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. ribonucleoside-diphosphate reductase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 329329Ribonucleoside-diphosphate reductase subunit betaPRO_0000389011Add
    BLAST

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO64174.
    SMRiO64174. Positions 5-286.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di1.10.620.20. 1 hit.
    InterProiIPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR026023. RNR_bsu_prok.
    IPR026494. RNR_NrdF.
    IPR000358. RNR_small.
    [Graphical view]
    PANTHERiPTHR23409. PTHR23409. 1 hit.
    PfamiPF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000355. NrdB. 1 hit.
    SUPFAMiSSF47240. SSF47240. 1 hit.
    TIGRFAMsiTIGR04171. RNR_1b_NrdF. 1 hit.
    PROSITEiPS00368. RIBORED_SMALL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O64174-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKIYDAANW SKHEDDFTQM FYNQNVKQFW LPEEIALNGD LLTWKYLGKN    50
    EQDTYMKVLA GLTLLDTEQG NTGMPIVAEH VDGHQRKAVL NFMAMMENAV 100
    HAKSYSNIFL TLAPTEQINE VFEWVKNNRF LQKKARTIVS VYKTIKKNDE 150
    ISLFKGMVAS VFLESFLFYS GFYYPLYFYG QGKLMQSGEI INLIIRDEAI 200
    HGVYVGLLAQ EIYKKQTPQK QKELYAWALN LLQELYENEL EYTEDVYDQV 250
    GLAPDVKKFI RYNANKALNN LGFDHWFEEE DVNPIVINGL NTKTKSHDFF 300
    STKGNGYKKA TVEPLKDSDF IFTEKGCIQ 329
    Length:329
    Mass (Da):38,181
    Last modified:August 1, 1998 - v1
    Checksum:iEC36E9DFC12BD269
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF020713 Genomic DNA. Translation: AAC13135.1.
    PIRiT12926.
    RefSeqiNP_046714.1. NC_001884.1.

    Genome annotation databases

    GeneIDi1261446.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF020713 Genomic DNA. Translation: AAC13135.1 .
    PIRi T12926.
    RefSeqi NP_046714.1. NC_001884.1.

    3D structure databases

    ProteinModelPortali O64174.
    SMRi O64174. Positions 5-286.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1261446.

    Enzyme and pathway databases

    UniPathwayi UPA00326 .

    Family and domain databases

    Gene3Di 1.10.620.20. 1 hit.
    InterProi IPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR026023. RNR_bsu_prok.
    IPR026494. RNR_NrdF.
    IPR000358. RNR_small.
    [Graphical view ]
    PANTHERi PTHR23409. PTHR23409. 1 hit.
    Pfami PF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000355. NrdB. 1 hit.
    SUPFAMi SSF47240. SSF47240. 1 hit.
    TIGRFAMsi TIGR04171. RNR_1b_NrdF. 1 hit.
    PROSITEi PS00368. RIBORED_SMALL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Introns and intein coding sequence in the ribonucleotide reductase genes of Bacillus subtilis temperate bacteriophage SPbeta."
      Lazarevic V., Soldo B., Duesterhoeft A., Hilbert H., Maueel C., Karamata D.
      Proc. Natl. Acad. Sci. U.S.A. 95:1692-1697(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], SPLICING.

    Entry informationi

    Entry nameiBNRDF_BPSPB
    AccessioniPrimary (citable) accession number: O64174
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This gene has an intron within which is encoded yosQ, a putative homing endonuclease.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3