SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O64174

- BNRDF_BPSPB

UniProt

O64174 - BNRDF_BPSPB

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Ribonucleoside-diphosphate reductase subunit beta
Gene
bnrdF, yosP
Organism
Bacillus phage SPbeta (Bacillus phage SPBc2) (Bacteriophage SP-beta)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

Binds 2 iron ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi66 – 661Iron 1 By similarity
Metal bindingi97 – 971Iron 1 By similarity
Metal bindingi97 – 971Iron 2 By similarity
Metal bindingi101 – 1011Iron 1 By similarity
Active sitei105 – 1051 By similarity
Metal bindingi164 – 1641Iron 2 By similarity
Metal bindingi198 – 1981Iron 2 By similarity
Metal bindingi201 – 2011Iron 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
  2. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  3. deoxyribonucleotide biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit beta (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Gene namesi
Name:bnrdF
Synonyms:yosP
OrganismiBacillus phage SPbeta (Bacillus phage SPBc2) (Bacteriophage SP-beta)
Taxonomic identifieri66797 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeSpbetalikevirus
Virus hostiBacillus pumilus (Bacillus mesentericus) [TaxID: 1408]
Bacillus subtilis [TaxID: 1423]
ProteomesiUP000009091: Genome

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329Ribonucleoside-diphosphate reductase subunit beta
PRO_0000389011Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits By similarity.

Structurei

3D structure databases

ProteinModelPortaliO64174.
SMRiO64174. Positions 5-286.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR026023. RNR_bsu_prok.
IPR026494. RNR_NrdF.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
PIRSFiPIRSF000355. NrdB. 1 hit.
SUPFAMiSSF47240. SSF47240. 1 hit.
TIGRFAMsiTIGR04171. RNR_1b_NrdF. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O64174-1 [UniParc]FASTAAdd to Basket

« Hide

MTKIYDAANW SKHEDDFTQM FYNQNVKQFW LPEEIALNGD LLTWKYLGKN    50
EQDTYMKVLA GLTLLDTEQG NTGMPIVAEH VDGHQRKAVL NFMAMMENAV 100
HAKSYSNIFL TLAPTEQINE VFEWVKNNRF LQKKARTIVS VYKTIKKNDE 150
ISLFKGMVAS VFLESFLFYS GFYYPLYFYG QGKLMQSGEI INLIIRDEAI 200
HGVYVGLLAQ EIYKKQTPQK QKELYAWALN LLQELYENEL EYTEDVYDQV 250
GLAPDVKKFI RYNANKALNN LGFDHWFEEE DVNPIVINGL NTKTKSHDFF 300
STKGNGYKKA TVEPLKDSDF IFTEKGCIQ 329
Length:329
Mass (Da):38,181
Last modified:August 1, 1998 - v1
Checksum:iEC36E9DFC12BD269
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF020713 Genomic DNA. Translation: AAC13135.1.
PIRiT12926.
RefSeqiNP_046714.1. NC_001884.1.

Genome annotation databases

GeneIDi1261446.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF020713 Genomic DNA. Translation: AAC13135.1 .
PIRi T12926.
RefSeqi NP_046714.1. NC_001884.1.

3D structure databases

ProteinModelPortali O64174.
SMRi O64174. Positions 5-286.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1261446.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

Gene3Di 1.10.620.20. 1 hit.
InterProi IPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR026023. RNR_bsu_prok.
IPR026494. RNR_NrdF.
IPR000358. RNR_small.
[Graphical view ]
PANTHERi PTHR23409. PTHR23409. 1 hit.
Pfami PF00268. Ribonuc_red_sm. 1 hit.
[Graphical view ]
PIRSFi PIRSF000355. NrdB. 1 hit.
SUPFAMi SSF47240. SSF47240. 1 hit.
TIGRFAMsi TIGR04171. RNR_1b_NrdF. 1 hit.
PROSITEi PS00368. RIBORED_SMALL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Introns and intein coding sequence in the ribonucleotide reductase genes of Bacillus subtilis temperate bacteriophage SPbeta."
    Lazarevic V., Soldo B., Duesterhoeft A., Hilbert H., Maueel C., Karamata D.
    Proc. Natl. Acad. Sci. U.S.A. 95:1692-1697(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], SPLICING.

Entry informationi

Entry nameiBNRDF_BPSPB
AccessioniPrimary (citable) accession number: O64174
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: August 1, 1998
Last modified: September 3, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

This gene has an intron within which is encoded yosQ, a putative homing endonuclease.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi