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O64174 (BNRDF_BPSPB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonucleoside-diphosphate reductase subunit beta

EC=1.17.4.1
Alternative name(s):
Ribonucleotide reductase small subunit
Gene names
Name:bnrdF
Synonyms:yosP
OrganismBacillus phage SPbeta (Bacillus phage SPBc2) (Bacteriophage SP-beta) [Reference proteome]
Taxonomic identifier66797 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeSpbetalikevirus
Virus hostBacillus pumilus (Bacillus mesentericus) [TaxID: 1408]
Bacillus subtilis [TaxID: 1423]

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactor

Binds 2 iron ions per subunit By similarity.

Pathway

Genetic information processing; DNA replication.

Subunit structure

Tetramer of two alpha and two beta subunits By similarity.

Miscellaneous

This gene has an intron within which is encoded yosQ, a putative homing endonuclease.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase small chain family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329Ribonucleoside-diphosphate reductase subunit beta
PRO_0000389011

Sites

Active site1051 By similarity
Metal binding661Iron 1 By similarity
Metal binding971Iron 1 By similarity
Metal binding971Iron 2 By similarity
Metal binding1011Iron 1 By similarity
Metal binding1641Iron 2 By similarity
Metal binding1981Iron 2 By similarity
Metal binding2011Iron 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
O64174 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: EC36E9DFC12BD269

FASTA32938,181
        10         20         30         40         50         60 
MTKIYDAANW SKHEDDFTQM FYNQNVKQFW LPEEIALNGD LLTWKYLGKN EQDTYMKVLA 

        70         80         90        100        110        120 
GLTLLDTEQG NTGMPIVAEH VDGHQRKAVL NFMAMMENAV HAKSYSNIFL TLAPTEQINE 

       130        140        150        160        170        180 
VFEWVKNNRF LQKKARTIVS VYKTIKKNDE ISLFKGMVAS VFLESFLFYS GFYYPLYFYG 

       190        200        210        220        230        240 
QGKLMQSGEI INLIIRDEAI HGVYVGLLAQ EIYKKQTPQK QKELYAWALN LLQELYENEL 

       250        260        270        280        290        300 
EYTEDVYDQV GLAPDVKKFI RYNANKALNN LGFDHWFEEE DVNPIVINGL NTKTKSHDFF 

       310        320 
STKGNGYKKA TVEPLKDSDF IFTEKGCIQ 

« Hide

References

[1]"Introns and intein coding sequence in the ribonucleotide reductase genes of Bacillus subtilis temperate bacteriophage SPbeta."
Lazarevic V., Soldo B., Duesterhoeft A., Hilbert H., Maueel C., Karamata D.
Proc. Natl. Acad. Sci. U.S.A. 95:1692-1697(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], SPLICING.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF020713 Genomic DNA. Translation: AAC13135.1.
PIRT12926.
RefSeqNP_046714.1. NC_001884.1.

3D structure databases

ProteinModelPortalO64174.
SMRO64174. Positions 5-286.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1261446.

Enzyme and pathway databases

UniPathwayUPA00326.

Family and domain databases

Gene3D1.10.620.20. 1 hit.
InterProIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR026494. RNR_1b_NrdF.
IPR026023. RNR_bsu_prok.
IPR000358. RNR_small.
[Graphical view]
PANTHERPTHR23409. PTHR23409. 1 hit.
PfamPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
PIRSFPIRSF000355. NrdB. 1 hit.
SUPFAMSSF47240. SSF47240. 1 hit.
TIGRFAMsTIGR04171. RNR_1b_NrdF. 1 hit.
PROSITEPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBNRDF_BPSPB
AccessionPrimary (citable) accession number: O64174
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: August 1, 1998
Last modified: May 14, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways