O63066 (SECY_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Preprotein translocase subunit SECY, chloroplastic

Alternative name(s):
CpSecY

Gene names

Name:	SECY
Synonyms:	CSY1

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	553 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The central subunit of the protein translocation channel SecYE. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug By similarity.
Subunit structure	Component of the plastid Sec protein translocase complex, which is composed of at least SECY and SECE By similarity.
Subcellular location	Plastid › chloroplast thylakoid membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the SecY/SEC61-alpha family.
Sequence caution	The sequence AAB96657.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Chloroplast Membrane Plastid Thylakoid
Domain	Transit peptide Transmembrane Transmembrane helix
Gene Ontology (GO)
Cellular component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	P-P-bond-hydrolysis-driven protein transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 553		Preprotein translocase subunit SECY, chloroplastic		PRO_0000031996
Regions
	Transmembrane	144 – 164	21	Helical; Potential
	Transmembrane	194 – 214	21	Helical; Potential
	Transmembrane	243 – 263	21	Helical; Potential
	Transmembrane	270 – 290	21	Helical; Potential
	Transmembrane	297 – 317	21	Helical; Potential
	Transmembrane	330 – 350	21	Helical; Potential
	Transmembrane	384 – 404	21	Helical; Potential
	Transmembrane	417 – 437	21	Helical; Potential
	Transmembrane	482 – 502	21	Helical; Potential
	Transmembrane	505 – 525	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	O63066 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 641A3E5D3F340417	FASTA	553	59,354
	10 20 30 40 50 60 MVTATTTPPQ CWRGLPALAR APPPLSSPLR PRAVAAAAPI YPLRRRIAIT TTRGRRGTLD 70 80 90 100 110 120 CSPRCTLETA GPGFDPLGLY EEGSNSLSRS PLSTFFGIMA PVFGSSSGGG ASREKASGRG 130 140 150 160 170 180 AAAAIEDSSI DIGDFFKGPL PGKFLKLLGF LALSRLGVYI PLGGVNREAF AGNLDQNSLL 190 200 210 220 230 240 GTLDSFSGGG IGRLGICSLG IVPFINAQIV FQLLAQLYPK LQDLQRKEGE AGRKKVLQYT 250 260 270 280 290 300 RYASVGFAIV QAIGQVLYLR PYVNDFSTEW VLTSVTLLTL GSVFTTFIGE RISDLKLGNG 310 320 330 340 350 360 TSLLIFTSII SYLPASFGRT VAQAFQDGNY VGLLTIILSF LLLVLGIVYV QEAERKIPLN 370 380 390 400 410 420 YASRYSSRTG ELQRSAYLPF KVNSSGVMPI IFSTSSLALP GTLARFSGLD FLKKAAIALN 430 440 450 460 470 480 PGGALYLPTN VLLIAFFNYY YTFLQLDPDD LSEQLKRQGA SIPLVRPGKS TAAYIKTVLN 490 500 510 520 530 540 RISVLGSAFL AVLAAGPSLV EQTSHLTAFR GFAGTSVLIL VGCATDTARK VQAEIISQKY 550 KNIEFYDVNR FGQ

References

[1]	"A SecY homologue is required for the elaboration of the chloroplast thylakoid membrane and for normal chloroplast gene expression." Roy L.M., Barkan A. J. Cell Biol. 141:385-395(1998) [PubMed: 9548717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. B73.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF039304 mRNA. Translation: AAC05019.1. AF039305 Genomic DNA. Translation: AAB96657.1. Sequence problems.
PIR	T01416. T01432.
RefSeq	NP_001105505.1. NM_001112035.1.
UniGene	Zm.94586.
3D structure databases
ProteinModelPortal	O63066.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	542482.
KEGG	zma:542482.
Organism-specific databases
Gramene	O63066.
Phylogenomic databases
GeneTree	EPGT00050000018925.
Family and domain databases
InterPro	IPR002208. SecY. IPR023201. SecY_su_dom. [Graphical view]
Gene3D	G3DSA:1.10.3370.10. G3DSA:1.10.3370.10. 1 hit.
PANTHER	PTHR10906. SecY. 1 hit.
Pfam	PF00344. SecY. 1 hit. [Graphical view]
PRINTS	PR00303. SECYTRNLCASE.
SUPFAM	SSF103491. SecY. 1 hit.
TIGRFAMs	TIGR00967. 3a0501s007. 1 hit.
PROSITE	PS00755. SECY_1. 1 hit. PS00756. SECY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SECY_MAIZE

Accession

Primary (citable) accession number: O63066
Secondary accession number(s): O47005

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program