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O62830

- PPM1B_BOVIN

UniProt

O62830 - PPM1B_BOVIN

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Protein

Protein phosphatase 1B

Gene

PPM1B

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Enzyme with a broad specificity. Dephosphorylates PRKAA1 and PRKAA2. Inhibits TBK1-mediated antiviral signaling by dephosphorylating it at 'Ser-172'. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601Manganese 1By similarity
Metal bindingi60 – 601Manganese 2By similarity
Metal bindingi61 – 611Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi243 – 2431Manganese 2By similarity
Metal bindingi286 – 2861Manganese 2By similarity

GO - Molecular functioni

  1. magnesium ion binding Source: InterPro
  2. manganese ion binding Source: InterPro
  3. protein serine/threonine phosphatase activity Source: InterPro

GO - Biological processi

  1. negative regulation of defense response to virus Source: UniProtKB
  2. negative regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  3. negative regulation of interferon-beta production Source: UniProtKB
  4. negative regulation of NF-kappaB import into nucleus Source: UniProtKB
  5. N-terminal protein myristoylation Source: UniProtKB
  6. peptidyl-threonine dephosphorylation Source: Ensembl
  7. protein dephosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_215264. ISG15 antiviral mechanism.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1B (EC:3.1.3.16)
Alternative name(s):
Protein phosphatase 2C isoform beta
Short name:
PP2C-beta
Gene namesi
Name:PPM1B
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 11

Subcellular locationi

Cytoplasmcytosol By similarity. Membrane By similarity
Note: Weakly associates at the membrane and N-myristoylation mediates the membrane localization.By similarity

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 484483Protein phosphatase 1BPRO_0000057745Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Cross-linki12 – 12Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)By similarity
Cross-linki142 – 142Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)By similarity

Post-translational modificationi

Isgylation negatively regulates its activity.By similarity
N-myristoylation is essential for the recognition of its substrates for dephosphorylation.By similarity

Keywords - PTMi

Isopeptide bond, Lipoprotein, Myristate, Ubl conjugation

Proteomic databases

PRIDEiO62830.

Interactioni

Subunit structurei

Monomer. Interacts with PAK6. Interacts with the phosphorylated form of IKBKB/IKKB.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000043518.

Structurei

3D structure databases

ProteinModelPortaliO62830.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00740000115384.
HOGENOMiHOG000233895.
HOVERGENiHBG053647.
InParanoidiO62830.
KOiK04461.
OMAiVMISPEH.
OrthoDBiEOG7WMCJH.
TreeFamiTF313590.

Family and domain databases

Gene3Di1.10.10.430. 1 hit.
3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR012911. PP2C_C.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
PF07830. PP2C_C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81601. SSF81601. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Beta-1 (identifier: O62830-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAFLDKPKT EKHNAHGAGN GLRYGLSSMQ GWRVEMEDAH TAVVGIPHGL
60 70 80 90 100
EDWSFFAVYD GHAGSRVANY CSTHLLEHIT NNEDFRAAGK SGSALEPSVE
110 120 130 140 150
NVKNGIRTGF LKIDEYMRNF SDLRNGMDRS GSTAVGVMIS PKHIYFINCG
160 170 180 190 200
DSRAVLYRSG QVCFSTQDHK PCNPREKERI QNAGGSVMIQ RVNGSLAVSR
210 220 230 240 250
ALGDYDYKCV DGKGPTEQLV SPEPEVYEIL RAEEDEFIIL ACDGIWDVMS
260 270 280 290 300
NEELCEFVKS RLEVSDDLEN VCNWVVDTCL HKGSRDNMSI VLVCFSNAPK
310 320 330 340 350
VSDEAMRKDS ELDKYLESRV EEIMEKSGEE GMPDLAHVMR ILSAENIPNL
360 370 380 390 400
PPGGGLAGNI IFFRRHVIEA VYSRLNPHRE SDGASDEAEE SGSQGKLVEA
410 420 430 440 450
LRQMRINHRG NYRQLLEEML TSYRLAKVEG EENPAEQAAT AASSNSDAGN
460 470 480
TVAMQESHTE SKSDLAELDS CTEDAGTKMS GEKL
Length:484
Mass (Da):53,431
Last modified:July 25, 2006 - v2
Checksum:i453DD1E6E7D47D27
GO
Isoform Beta-2 (identifier: O62830-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     359-364: NIIFFR → K
     384-392: ASDEAEESG → GAGDLEDPW
     393-484: Missing.

Show »
Length:387
Mass (Da):42,834
Checksum:iD70B95141250FFB1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei359 – 3646NIIFFR → K in isoform Beta-2. 1 PublicationVSP_020009
Alternative sequencei384 – 3929ASDEAEESG → GAGDLEDPW in isoform Beta-2. 1 PublicationVSP_020010
Alternative sequencei393 – 48492Missing in isoform Beta-2. 1 PublicationVSP_020011Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005458 mRNA. Translation: CAA06555.1.
BC111235 mRNA. Translation: AAI11236.1.
RefSeqiNP_776855.1. NM_174430.2. [O62830-2]
UniGeneiBt.5004.

Genome annotation databases

EnsembliENSBTAT00000046197; ENSBTAP00000043518; ENSBTAG00000000223. [O62830-1]
ENSBTAT00000050064; ENSBTAP00000046832; ENSBTAG00000000223. [O62830-2]
GeneIDi281995.
KEGGibta:281995.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005458 mRNA. Translation: CAA06555.1 .
BC111235 mRNA. Translation: AAI11236.1 .
RefSeqi NP_776855.1. NM_174430.2. [O62830-2 ]
UniGenei Bt.5004.

3D structure databases

ProteinModelPortali O62830.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000043518.

Proteomic databases

PRIDEi O62830.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000046197 ; ENSBTAP00000043518 ; ENSBTAG00000000223 . [O62830-1 ]
ENSBTAT00000050064 ; ENSBTAP00000046832 ; ENSBTAG00000000223 . [O62830-2 ]
GeneIDi 281995.
KEGGi bta:281995.

Organism-specific databases

CTDi 5495.

Phylogenomic databases

eggNOGi COG0631.
GeneTreei ENSGT00740000115384.
HOGENOMi HOG000233895.
HOVERGENi HBG053647.
InParanoidi O62830.
KOi K04461.
OMAi VMISPEH.
OrthoDBi EOG7WMCJH.
TreeFami TF313590.

Enzyme and pathway databases

Reactomei REACT_215264. ISG15 antiviral mechanism.

Miscellaneous databases

NextBioi 20805863.

Family and domain databases

Gene3Di 1.10.10.430. 1 hit.
3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR012911. PP2C_C.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
PF07830. PP2C_C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81601. SSF81601. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Protein phosphatase type-2C isozymes present in vertebrate retinae: purification, characterization, and localization in photoreceptors."
    Klumpp S., Selke D., Fischer D., Baumann A., Mueller F., Thanos S.
    J. Neurosci. Res. 51:328-338(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA-2).
    Tissue: Retina.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BETA-1).
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiPPM1B_BOVIN
AccessioniPrimary (citable) accession number: O62830
Secondary accession number(s): Q2T9W5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 25, 2006
Last modified: October 1, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3