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Protein

Protein phosphatase 1B

Gene

PPM1B

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Enzyme with a broad specificity. Dephosphorylates PRKAA1 and PRKAA2. Inhibits TBK1-mediated antiviral signaling by dephosphorylating it at 'Ser-172'. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60Manganese 1By similarity1
Metal bindingi60Manganese 2By similarity1
Metal bindingi61Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi243Manganese 2By similarity1
Metal bindingi286Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-1169408 ISG15 antiviral mechanism

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1B (EC:3.1.3.16)
Alternative name(s):
Protein phosphatase 2C isoform beta
Short name:
PP2C-beta
Gene namesi
Name:PPM1B
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 11

Organism-specific databases

VGNCiVGNC:33209 PPM1B

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000577452 – 484Protein phosphatase 1BAdd BLAST483

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Cross-linki12Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)By similarity
Cross-linki142Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)By similarity
Modified residuei391PhosphoserineBy similarity1

Post-translational modificationi

Isgylation negatively regulates its activity.By similarity
N-myristoylation is essential for the recognition of its substrates for dephosphorylation.By similarity

Keywords - PTMi

Isopeptide bond, Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO62830
PRIDEiO62830

Expressioni

Gene expression databases

BgeeiENSBTAG00000000223

Interactioni

Subunit structurei

Monomer. Interacts with PAK6. Interacts with the phosphorylated form of IKBKB/IKKB.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000043518

Structurei

3D structure databases

ProteinModelPortaliO62830
SMRiO62830
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 295PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST273

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiKOG0697 Eukaryota
COG0631 LUCA
GeneTreeiENSGT00880000137909
HOGENOMiHOG000233895
HOVERGENiHBG053647
InParanoidiO62830
KOiK04461
OMAiXIMEKSG
OrthoDBiEOG091G0AJQ
TreeFamiTF313590

Family and domain databases

CDDicd00143 PP2Cc, 1 hit
Gene3Di1.10.10.430, 1 hit
3.60.40.10, 1 hit
InterProiView protein in InterPro
IPR015655 PP2C
IPR000222 PP2C_BS
IPR012911 PP2C_C
IPR036580 PP2C_C_sf
IPR036457 PPM-type_dom_sf
IPR001932 PPM-type_phosphatase_dom
PANTHERiPTHR13832 PTHR13832, 1 hit
PfamiView protein in Pfam
PF00481 PP2C, 1 hit
PF07830 PP2C_C, 1 hit
SMARTiView protein in SMART
SM00332 PP2Cc, 1 hit
SUPFAMiSSF81601 SSF81601, 1 hit
SSF81606 SSF81606, 1 hit
PROSITEiView protein in PROSITE
PS01032 PPM_1, 1 hit
PS51746 PPM_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Beta-1 (identifier: O62830-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAFLDKPKT EKHNAHGAGN GLRYGLSSMQ GWRVEMEDAH TAVVGIPHGL
60 70 80 90 100
EDWSFFAVYD GHAGSRVANY CSTHLLEHIT NNEDFRAAGK SGSALEPSVE
110 120 130 140 150
NVKNGIRTGF LKIDEYMRNF SDLRNGMDRS GSTAVGVMIS PKHIYFINCG
160 170 180 190 200
DSRAVLYRSG QVCFSTQDHK PCNPREKERI QNAGGSVMIQ RVNGSLAVSR
210 220 230 240 250
ALGDYDYKCV DGKGPTEQLV SPEPEVYEIL RAEEDEFIIL ACDGIWDVMS
260 270 280 290 300
NEELCEFVKS RLEVSDDLEN VCNWVVDTCL HKGSRDNMSI VLVCFSNAPK
310 320 330 340 350
VSDEAMRKDS ELDKYLESRV EEIMEKSGEE GMPDLAHVMR ILSAENIPNL
360 370 380 390 400
PPGGGLAGNI IFFRRHVIEA VYSRLNPHRE SDGASDEAEE SGSQGKLVEA
410 420 430 440 450
LRQMRINHRG NYRQLLEEML TSYRLAKVEG EENPAEQAAT AASSNSDAGN
460 470 480
TVAMQESHTE SKSDLAELDS CTEDAGTKMS GEKL
Length:484
Mass (Da):53,431
Last modified:July 25, 2006 - v2
Checksum:i453DD1E6E7D47D27
GO
Isoform Beta-2 (identifier: O62830-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     359-364: NIIFFR → K
     384-392: ASDEAEESG → GAGDLEDPW
     393-484: Missing.

Show »
Length:387
Mass (Da):42,834
Checksum:iD70B95141250FFB1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020009359 – 364NIIFFR → K in isoform Beta-2. 1 Publication6
Alternative sequenceiVSP_020010384 – 392ASDEAEESG → GAGDLEDPW in isoform Beta-2. 1 Publication9
Alternative sequenceiVSP_020011393 – 484Missing in isoform Beta-2. 1 PublicationAdd BLAST92

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005458 mRNA Translation: CAA06555.1
BC111235 mRNA Translation: AAI11236.1
RefSeqiNP_776855.1, NM_174430.2 [O62830-2]
UniGeneiBt.5004

Genome annotation databases

EnsembliENSBTAT00000046197; ENSBTAP00000043518; ENSBTAG00000000223 [O62830-1]
ENSBTAT00000050064; ENSBTAP00000046832; ENSBTAG00000000223 [O62830-2]
GeneIDi281995
KEGGibta:281995

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPPM1B_BOVIN
AccessioniPrimary (citable) accession number: O62830
Secondary accession number(s): Q2T9W5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 25, 2006
Last modified: March 28, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health