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O62830 (PPM1B_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 1B

EC=3.1.3.16
Alternative name(s):
Protein phosphatase 2C isoform beta
Short name=PP2C-beta
Gene names
Name:PPM1B
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length484 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Enzyme with a broad specificity. Dephosphorylates PRKAA1 and PRKAA2. Inhibits TBK1-mediated antiviral signaling by dephosphorylating it at 'Ser-172'. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB By similarity.

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Subunit structure

Monomer By similarity. Interacts with PAK6 By similarity. Interacts with the phosphorylated form of IKBKB/IKKB By similarity.

Subcellular location

Cytoplasmcytosol By similarity. Membrane By similarity. Note: Weakly associates at the membrane and N-myristoylation mediates the membrane localization By similarity.

Post-translational modification

Isgylation negatively regulates its activity By similarity.

N-myristoylation is essential for the recognition of its substrates for dephosphorylation By similarity.

Sequence similarities

Belongs to the PP2C family.

Ontologies

Keywords
   Cellular componentCytoplasm
Membrane
   Coding sequence diversityAlternative splicing
   LigandMagnesium
Manganese
Metal-binding
   Molecular functionHydrolase
Protein phosphatase
   PTMIsopeptide bond
Lipoprotein
Myristate
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processN-terminal protein myristoylation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of I-kappaB kinase/NF-kappaB signaling

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of NF-kappaB import into nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of defense response to virus

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of interferon-beta production

Inferred from sequence or structural similarity. Source: UniProtKB

peptidyl-threonine dephosphorylation

Inferred from electronic annotation. Source: Ensembl

protein dephosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytosol

Inferred from sequence or structural similarity. Source: UniProtKB

membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionmagnesium ion binding

Inferred from electronic annotation. Source: InterPro

manganese ion binding

Inferred from electronic annotation. Source: InterPro

protein serine/threonine phosphatase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Beta-1 (identifier: O62830-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Beta-2 (identifier: O62830-2)

The sequence of this isoform differs from the canonical sequence as follows:
     359-364: NIIFFR → K
     384-392: ASDEAEESG → GAGDLEDPW
     393-484: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 484483Protein phosphatase 1B
PRO_0000057745

Sites

Metal binding601Manganese 1 By similarity
Metal binding601Manganese 2 By similarity
Metal binding611Manganese 1; via carbonyl oxygen By similarity
Metal binding2431Manganese 2 By similarity
Metal binding2861Manganese 2 By similarity

Amino acid modifications

Lipidation21N-myristoyl glycine By similarity
Cross-link12Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15) By similarity
Cross-link142Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15) By similarity

Natural variations

Alternative sequence359 – 3646NIIFFR → K in isoform Beta-2.
VSP_020009
Alternative sequence384 – 3929ASDEAEESG → GAGDLEDPW in isoform Beta-2.
VSP_020010
Alternative sequence393 – 48492Missing in isoform Beta-2.
VSP_020011

Sequences

Sequence LengthMass (Da)Tools
Isoform Beta-1 [UniParc].

Last modified July 25, 2006. Version 2.
Checksum: 453DD1E6E7D47D27

FASTA48453,431
        10         20         30         40         50         60 
MGAFLDKPKT EKHNAHGAGN GLRYGLSSMQ GWRVEMEDAH TAVVGIPHGL EDWSFFAVYD 

        70         80         90        100        110        120 
GHAGSRVANY CSTHLLEHIT NNEDFRAAGK SGSALEPSVE NVKNGIRTGF LKIDEYMRNF 

       130        140        150        160        170        180 
SDLRNGMDRS GSTAVGVMIS PKHIYFINCG DSRAVLYRSG QVCFSTQDHK PCNPREKERI 

       190        200        210        220        230        240 
QNAGGSVMIQ RVNGSLAVSR ALGDYDYKCV DGKGPTEQLV SPEPEVYEIL RAEEDEFIIL 

       250        260        270        280        290        300 
ACDGIWDVMS NEELCEFVKS RLEVSDDLEN VCNWVVDTCL HKGSRDNMSI VLVCFSNAPK 

       310        320        330        340        350        360 
VSDEAMRKDS ELDKYLESRV EEIMEKSGEE GMPDLAHVMR ILSAENIPNL PPGGGLAGNI 

       370        380        390        400        410        420 
IFFRRHVIEA VYSRLNPHRE SDGASDEAEE SGSQGKLVEA LRQMRINHRG NYRQLLEEML 

       430        440        450        460        470        480 
TSYRLAKVEG EENPAEQAAT AASSNSDAGN TVAMQESHTE SKSDLAELDS CTEDAGTKMS 


GEKL 

« Hide

Isoform Beta-2 [UniParc].

Checksum: D70B95141250FFB1
Show »

FASTA38742,834

References

« Hide 'large scale' references
[1]"Protein phosphatase type-2C isozymes present in vertebrate retinae: purification, characterization, and localization in photoreceptors."
Klumpp S., Selke D., Fischer D., Baumann A., Mueller F., Thanos S.
J. Neurosci. Res. 51:328-338(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA-2).
Tissue: Retina.
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BETA-1).
Strain: Crossbred X Angus.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ005458 mRNA. Translation: CAA06555.1.
BC111235 mRNA. Translation: AAI11236.1.
RefSeqNP_776855.1. NM_174430.2. [O62830-2]
UniGeneBt.5004.

3D structure databases

ProteinModelPortalO62830.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000043518.

Proteomic databases

PRIDEO62830.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000046197; ENSBTAP00000043518; ENSBTAG00000000223. [O62830-1]
ENSBTAT00000050064; ENSBTAP00000046832; ENSBTAG00000000223. [O62830-2]
GeneID281995.
KEGGbta:281995.

Organism-specific databases

CTD5495.

Phylogenomic databases

eggNOGCOG0631.
GeneTreeENSGT00740000115384.
HOGENOMHOG000233895.
HOVERGENHBG053647.
InParanoidO62830.
KOK04461.
OMAVMISPEH.
OrthoDBEOG7WMCJH.
TreeFamTF313590.

Family and domain databases

Gene3D1.10.10.430. 1 hit.
3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like_dom.
IPR012911. PP2C_C.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
PF07830. PP2C_C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81601. SSF81601. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805863.

Entry information

Entry namePPM1B_BOVIN
AccessionPrimary (citable) accession number: O62830
Secondary accession number(s): Q2T9W5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 25, 2006
Last modified: May 14, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families