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O62829 (PPM1A_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Protein phosphatase 1A
EC=3.1.3.16
Alternative name(s):
Protein phosphatase 2C isoform alpha
Short name=PP2C-alpha
Gene names
Name:PPM1A
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling By similarity.

Catalytic activity

A phosphoprotein + H2O = a protein + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit.

Subunit structure

Monomer. Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling. Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the PP2C family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 382382Protein phosphatase 1A
PRO_0000057740

Sites

Metal binding601Manganese 1 By similarity
Metal binding601Manganese 2 By similarity
Metal binding611Manganese 1; via carbonyl oxygen By similarity
Metal binding2391Manganese 2 By similarity
Metal binding2821Manganese 2 By similarity

Amino acid modifications

Modified residue3751Phosphoserine By similarity
Modified residue3771Phosphoserine By similarity
Lipidation21N-myristoyl glycine By similarity

Sequences

Sequence LengthMass (Da)Tools
O62829 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: A716B3FA0E7E21C2

FASTA38242,530
        10         20         30         40         50         60 
MGAFLDKPKM EKHNAQGQGN GLRYGLSSMQ GWRVEMEDAH TAVIGLPSGL ETWSFFAVYD 

        70         80         90        100        110        120 
GHAGSQVAKY CCEHLLDHIT NNQDFKGSAG APSVENVKNG IRTGFLEIDE HMRVMSEKKH 

       130        140        150        160        170        180 
GADRSGSTAV GVLISPQHTY FINCGDSRGL LCRNRKVYFF TQDHKPSNPL EKERIQNAGG 

       190        200        210        220        230        240 
SVMIQRVNGS LAVSRALGDF DYKCVHGKGP TEQLVSPEPE VHDIERSEED DQFIILACDG 

       250        260        270        280        290        300 
IWDVMGNEEL CDFVRSRLEV TDDLEKVCNE VVDTCLYKGS RDNMSVILIC FPNAPKVSPE 

       310        320        330        340        350        360 
AVKKEEELDK YLESRVEEII KKQGEGVPDL VHVMRTLASE NIPSLPPGGE LASKRNVIEA 

       370        380 
VYNRLNPYKN DDTDSTSTDD MW

« Hide

References

[1]"Protein phosphatase type-2C isozymes present in vertebrate retinae: purification, characterization, and localization in photoreceptors."
Klumpp S., Selke D., Fischer D., Baumann A., Mueller F., Thanos S.
J. Neurosci. Res. 51:328-338(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Retina.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ005457 mRNA. Translation: CAA06554.1.
IPIIPI00694034.
RefSeqNP_776854.1. NM_174429.2.
UniGeneBt.4671.

3D structure databases

ProteinModelPortalO62829.
SMRO62829. Positions 2-368.
ModBaseSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000024128.

Proteomic databases

PRIDEO62829.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000024128; ENSBTAP00000024128; ENSBTAG00000018127.
GeneID281994.
KEGGbta:281994.

Organism-specific databases

CTD5494.

Phylogenomic databases

eggNOGCOG0631.
GeneTreeENSGT00650000093052.
HOGENOMHOG000233895.
HOVERGENHBG053647.
InParanoidO62829.
KOK04457.
OMAEVYAIER.
OrthoDBEOG4GMTX1.

Family and domain databases

Gene3D1.10.10.430. 1 hit.
3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like.
IPR012911. PP2C_C.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
PF07830. PP2C_C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. PP2C-related. 1 hit.
SSF81601. PP2C_C. 1 hit.
PROSITEPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805862.

Entry information

Entry namePPM1A_BOVIN
AccessionPrimary (citable) accession number: O62829
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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