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Protein

Peroxisome proliferator-activated receptor gamma

Gene

PPARG

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels.By similarity

Enzyme regulationi

PDPK1 activates its transcriptional activity independently of its kinase activity.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi135 – 20975Nuclear receptorPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri138 – 15821NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri175 – 19723NR C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Receptor

Keywords - Biological processi

Biological rhythms, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-SSC-383280. Nuclear Receptor transcription pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisome proliferator-activated receptor gamma
Short name:
PPAR-gamma
Alternative name(s):
Nuclear receptor subfamily 1 group C member 3
Gene namesi
Name:PPARG
Synonyms:NR1C3
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 13

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation
  • Cytoplasm By similarity

  • Note: Redistributed from the nucleus to the cytosol through a MAP2K1/MEK1-dependent manner. NOCT enhances its nuclear translocation (By similarity).By similarity

GO - Cellular componenti

  • Golgi apparatus Source: Ensembl
  • nucleus Source: AgBase
  • RNA polymerase II transcription factor complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504Peroxisome proliferator-activated receptor gammaPRO_0000053495Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei111 – 1111Phosphoserine; by MAPKBy similarity

Post-translational modificationi

Phosphorylated at basal conditions and dephosphorylated when treated with the ligand. May be dephosphorylated by PPP5C. The phosphorylated form may be inactive and dephosphorylation induces adipogenic activity (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO62807.

Expressioni

Tissue specificityi

Highest expression in adipose tissue and lower in spleen. Very low levels in kidney, intestine, lung and muscle.

Gene expression databases

GenevisibleiO62807. SS.

Interactioni

Subunit structurei

Heterodimer with other nuclear receptors, such as RXRA. The heterodimer with the retinoic acid receptor RXRA is called adipocyte-specific transcription factor ARF6. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Interacts with coactivator PPARBP, leading to a mild increase in transcription of target genes. Interacts with NOCA7 in a ligand-inducible manner. Interacts with NCOA1 and NCOA2 LXXLL motifs. Interacts with ASXL1, ASXL2, DNTTIP2, FAM120B, MAP2K1/MEK1, NR0B2, PDPK1, PRDM16, PRMT2 and TGFB1I1. Interacts (when activated by agonist) with PPP5C. Interacts with HELZ2 and THRAP3; the interaction stimulates the transcriptional activity of PPARG. Interacts with PER2, the interaction is ligand dependent and blocks PPARG recruitment to target promoters. Interacts with NOCT. Interacts with FOXO1 (acetylated form). Interacts with ACTN4.By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000012339.

Structurei

3D structure databases

ProteinModelPortaliO62807.
SMRiO62807. Positions 130-215, 233-504.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni204 – 27976Interaction with FAM120BBy similarityAdd
BLAST
Regioni316 – 504189Ligand-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri138 – 15821NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri175 – 19723NR C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3575. Eukaryota.
ENOG410XRZC. LUCA.
GeneTreeiENSGT00840000129707.
HOGENOMiHOG000261626.
HOVERGENiHBG106004.
InParanoidiO62807.
KOiK08530.
OMAiSTPHYED.
OrthoDBiEOG7X9G7F.
TreeFamiTF316304.

Family and domain databases

Gene3Di1.10.565.10. 2 hits.
3.30.50.10. 1 hit.
InterProiIPR003074. 1Cnucl_rcpt.
IPR003077. 1Cnucl_rcpt_G.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR022590. PPARgamma_N.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF12577. PPARgamma_N. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR01288. PROXISOMEPAR.
PR01291. PROXISOMPAGR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: O62807-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGETLGDSLI DPESDAFDTL SANISQEVTM VDTEMPFWPT NFGISSVDLS
60 70 80 90 100
VMDDHSHSFD IKPFTTVDFS SISTPHYEDI PFPRADPMVA DYKYDLKLQD
110 120 130 140 150
YQSAIKVEPV SPPYYSEKTQ LYNKPHEEPS NSLMAIECRV CGDKASGFHY
160 170 180 190 200
GVHACEGCKG FFRRTIRLKL IYDRCDLNCR IHKKSRNKCQ YCRFQKCLAV
210 220 230 240 250
GMSHNAIRFG RMPQAEKEKL LAEISSDIDQ LNPESADLRA LAKHLYDSYI
260 270 280 290 300
KSFPLTKAKA RAILTGKTTD KSPFVIYDMN SLMMGEDKIK FKHITPLQEQ
310 320 330 340 350
SKEVAIRIFQ GCQFRSVEAV QEITEYAKNI PGFVNLDLND QVTLLKYGVH
360 370 380 390 400
EIIYTMLASL MNKDGVLISE GQGFMTREFL KSLRKPFGDF MEPKFEFAVK
410 420 430 440 450
FNALELDDSD LAIFIAVIIL SGDRPGLLNV KPIEDIQDNL LQALELQLKL
460 470 480 490 500
NHPESSQLFA KLLQKMTDLR QIVTEHVQLL QVIKKTETDM SLHPLLQEIY

KDLY
Length:504
Mass (Da):57,512
Last modified:August 1, 1998 - v1
Checksum:i57C0BDE901493D31
GO
Isoform 1 (identifier: O62807-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.

Show »
Length:475
Mass (Da):54,474
Checksum:i93B9D33CC64048D4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti381 – 3811K → R in CAA07224 (PubMed:9731203).Curated
Sequence conflicti381 – 3811K → R in CAA07225 (PubMed:9731203).Curated
Sequence conflicti426 – 4261G → R in CAA07224 (PubMed:9731203).Curated
Sequence conflicti426 – 4261G → R in CAA07225 (PubMed:9731203).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2929Missing in isoform 1. 2 PublicationsVSP_003648Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059245 mRNA. Translation: AAC14348.1.
AF103946 mRNA. Translation: AAD19577.1.
AJ006756 mRNA. Translation: CAA07224.1.
AJ006757 mRNA. Translation: CAA07225.1.
AB097930 mRNA. Translation: BAD20646.1.
PIRiJE0280.
RefSeqiNP_999544.1. NM_214379.1. [O62807-1]
UniGeneiSsc.14475.

Genome annotation databases

EnsembliENSSSCT00000012672; ENSSSCP00000012339; ENSSSCG00000011579. [O62807-1]
GeneIDi397671.
KEGGissc:397671.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059245 mRNA. Translation: AAC14348.1.
AF103946 mRNA. Translation: AAD19577.1.
AJ006756 mRNA. Translation: CAA07224.1.
AJ006757 mRNA. Translation: CAA07225.1.
AB097930 mRNA. Translation: BAD20646.1.
PIRiJE0280.
RefSeqiNP_999544.1. NM_214379.1. [O62807-1]
UniGeneiSsc.14475.

3D structure databases

ProteinModelPortaliO62807.
SMRiO62807. Positions 130-215, 233-504.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000012339.

Proteomic databases

PaxDbiO62807.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000012672; ENSSSCP00000012339; ENSSSCG00000011579. [O62807-1]
GeneIDi397671.
KEGGissc:397671.

Organism-specific databases

CTDi5468.

Phylogenomic databases

eggNOGiKOG3575. Eukaryota.
ENOG410XRZC. LUCA.
GeneTreeiENSGT00840000129707.
HOGENOMiHOG000261626.
HOVERGENiHBG106004.
InParanoidiO62807.
KOiK08530.
OMAiSTPHYED.
OrthoDBiEOG7X9G7F.
TreeFamiTF316304.

Enzyme and pathway databases

ReactomeiR-SSC-383280. Nuclear Receptor transcription pathway.

Gene expression databases

GenevisibleiO62807. SS.

Family and domain databases

Gene3Di1.10.565.10. 2 hits.
3.30.50.10. 1 hit.
InterProiIPR003074. 1Cnucl_rcpt.
IPR003077. 1Cnucl_rcpt_G.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR022590. PPARgamma_N.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF12577. PPARgamma_N. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR01288. PROXISOMEPAR.
PR01291. PROXISOMPAGR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression and cDNA cloning of porcine peroxisome proliferator-activated receptor gamma (PPARgamma)."
    Houseknecht K.L., Bidwell C.A., Portocarrero C.P., Spurlock M.E.
    Gene 225:89-96(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Tissue: Adipose tissue.
  2. "Expression of porcine adipocyte transcripts: tissue distribution and differentiation in vitro and in vivo."
    Ding S.T., McNeel R.L., Mersmann H.J.
    Comp. Biochem. Physiol. 123B:307-318(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  3. "Characterisation of porcine peroxisome proliferator-activated receptors gamma 1 and gamma 2: detection of breed and age differences in gene expression."
    Grindflek E., Sundvold H., Klungland H., Lien S.
    Biochem. Biophys. Res. Commun. 249:713-718(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Strain: Duroc and Norwegian Landrace.
    Tissue: Adipocyte.
  4. "Identification and characterization of novel peroxisome proliferator-activated receptor-gamma (PPAR-gamma) transcriptional variants in pig and human."
    Omi T., Brenig B., Kramer S.S., Iwamoto S., Stranzinger G., Neuenshwander S.
    J. Anim. Breed. Genet. 122:45-53(2005)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).

Entry informationi

Entry nameiPPARG_PIG
AccessioniPrimary (citable) accession number: O62807
Secondary accession number(s): O77815, Q6L9M2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: August 1, 1998
Last modified: May 11, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.