Reviewed, UniProtKB/Swiss-Prot O62768 (TRXR1_BOVIN)

Names and origin

Protein names

Recommended name:
    Thioredoxin reductase 1, cytoplasmic
      Short name=TR
    EC=1.8.1.9
Alternative name(s):
    Thioredoxin reductase TR1

Gene names

Name:

TXNRD1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The active site is a redox-active disulfide bond. The selenocysteine residue is also essential for catalytic activity By similarity.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Selenocysteine
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP Selenium
Molecular function	Oxidoreductase
PTM	Acetylation Disulfide bond Phosphoprotein
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro selenium binding Inferred from electronic annotation. Source: UniProtKB-KW thioredoxin-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Thioredoxin reductase 1, cytoplasmic		PRO_0000067981
Regions
	Nucleotide binding	42 – 59	18	FAD By similarity
Sites
	Active site	472	1	Proton acceptor By similarity
Amino acid modifications
	Non-standard residue	498	1	Selenocysteine
	Modified residue	11	1	Phosphotyrosine By similarity
	Modified residue	13	1	Phosphotyrosine By similarity
	Modified residue	131	1	Phosphotyrosine By similarity
	Modified residue	255	1	N6-acetyllysine By similarity
	Modified residue	422	1	Phosphotyrosine By similarity
	Disulfide bond	59 ↔ 64		Redox-active By similarity
	Cross-link	497 ↔ 498		Cysteinyl-selenocysteine (Cys-Sec) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O62768-1 [UniParc]. Last modified February 26, 2008. Version 3. Checksum: B0F15494D1795AB3	FASTA	499	54,770
	10 20 30 40 50 60 MNGSKDLPEP YDYDLIIIGG GSGGLAAAKE AAKYDKKVMV LDFVTPTPLG TRWGLGGTCV 70 80 90 100 110 120 NVGCIPKKLM HQAALLGQAL RDSRNYGWNV EETVKHDWER MTEAVQNHIG SLNWGYRVAL 130 140 150 160 170 180 REKKVTYENA YGEFVGPHRI KATNNKGKEK IYSAERFLIA TGERPRYLGI PGDKEYCISS 190 200 210 220 230 240 DDLFSLPYCP GKTLVVGASY VALECAGFLA GIGLDVTVMV RSILLRGFDQ DMANKIGEHM 250 260 270 280 290 300 QEHGIKFIRQ FVPIKVEQIE AGTPGRLRVI AKSTDSDQTI EGEYNTVLLA IGRDACTRKI 310 320 330 340 350 360 GLENVGVKIN EKTGKIPVTE EEQTNVPYIY AIGDILEGKL ELTPVAIQAG RLLAQRLYGG 370 380 390 400 410 420 STVKCDYENV PTTVFTPLEY GSCGLSEEKA VEKFGEENVE VYHSYFWPLE WTIPSRDNNK 430 440 450 460 470 480 CYAKVVCNIK DNERVVGFHV LGPNAGEVTQ GFAAALKCGL TKDQLDSTIG IHPVCAEVFT 490 TLSVTKRSGG NILQTGCUG

References

[1]	Terashima H. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Small intestine.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF053984 mRNA. Translation: AAC13914.1.
IPI	IPI00685161.
RefSeq	NP_777050.1.
UniGene	Bt.5534
3D structure databases
HSSP	HSSP built from PDB template 1ONF based on UniProtKB Q94655.
SMR	O62768. Positions 11-493.
ModBase	Search...
Protein-protein interaction databases
STRING	O62768.
Genome annotation databases
Ensembl	ENSBTAT00000018473; ENSBTAP00000018473; ENSBTAG00000013912; Bos taurus. [Genome view]
GeneID	282388.
KEGG	bta:282388.
Organism-specific databases
CTD	282388.
Phylogenomic databases
HOVERGEN	O62768.
Enzyme and pathway databases
BRENDA	1.8.1.9. 251.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR000815. Hg_reductase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR006338. Thioredoxin/glutathione_Rdtase. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
PANTHER	PTHR22912:SF23. Reduct_Se. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01438. TGR. 1 hit.
PROSITE	PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRXR1_BOVIN

Accession

Primary (citable) accession number: O62768

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 26, 2008
Last modified:	October 13, 2009
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)