Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O62763

- ACES_FELCA

UniProt

O62763 - ACES_FELCA

Protein

Acetylcholinesterase

Gene

ACHE

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.

    Catalytic activityi

    Acetylcholine + H2O = choline + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei231 – 2311Acyl-ester intermediatePROSITE-ProRule annotation
    Active sitei362 – 3621Charge relay systemBy similarity
    Active sitei475 – 4751Charge relay systemBy similarity

    GO - Molecular functioni

    1. acetylcholinesterase activity Source: UniProtKB-EC

    GO - Biological processi

    1. neurotransmitter catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Serine esterase

    Keywords - Biological processi

    Neurotransmitter degradation

    Protein family/group databases

    MEROPSiS09.979.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetylcholinesterase (EC:3.1.1.7)
    Short name:
    AChE
    Gene namesi
    Name:ACHE
    OrganismiFelis catus (Cat) (Felis silvestris catus)
    Taxonomic identifieri9685 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
    ProteomesiUP000011712: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. cell junction Source: UniProtKB-KW
    3. extracellular region Source: UniProtKB-SubCell
    4. plasma membrane Source: UniProtKB-SubCell
    5. synapse Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Secreted, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131By similarityAdd
    BLAST
    Chaini32 – 611580AcetylcholinesterasePRO_0000008586Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi97 ↔ 124By similarity
    Disulfide bondi285 ↔ 300By similarity
    Glycosylationi293 – 2931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi378 – 3781N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi437 ↔ 557By similarity
    Glycosylationi492 – 4921N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi608 – 608InterchainBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Interactioni

    Subunit structurei

    Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers By similarity. Isoform H generates GPI-anchored dimers; disulfide linked. Isoform T generates multiple structures, ranging from monomers and dimers to collagen-tailed and hydrophobic-tailed forms, in which catalytic tetramers are associated with anchoring proteins that attach them to the basal lamina or to cell membranes. In the collagen-tailed forms, isoform T subunits are associated with a specific collagen, COLQ, which triggers the formation of isoform T tetramers, from monomers and dimers.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO62763.
    SMRiO62763. Positions 33-605.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2272.
    HOVERGENiHBG008839.
    KOiK01049.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR014788. AChE_tetra.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    IPR000997. Cholinesterase.
    [Graphical view]
    PfamiPF08674. AChE_tetra. 1 hit.
    PF00135. COesterase. 1 hit.
    [Graphical view]
    PRINTSiPR00878. CHOLNESTRASE.
    ProDomiPD415333. AChE_tetra. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform T (identifier: O62763-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRPPWCPLYT PSLAAPILLL LLFLLGGGAE AEDPELLVTV RGGQLRGVRL    50
    MAPGGPVSAF LGIPFAEPPV GPRRFLPPEP KRPWPGVLDA TAFQSVCYQY 100
    VDTLYPGFEG TEMWNPNREL SEDCLYLNVW TPYPRPASPT PVLVWIYGGG 150
    FYSGASSLDV YDGRFLAQAE GTVLVSMNYR VGAFGFLALP GSREAPGNVG 200
    LLDQRLALQW VQDNVATFGG DPMSVTLFGE SAGAASVGMH LLSPPSRGLF 250
    HRAVLQSGAP NGPWATVGVG EARRRATLLA RLVGCPPGGA GGNDTELVAC 300
    LRTRPAQDLV DHEWHVLPQE SVFRFSFVPV VDGDFLSDTP EALINAGDFH 350
    GLQVLVGVVK DEGSYFLVYG APGFSKDNES LISRAQFLAG VRVGVPQASD 400
    LAAEAVVLHY TDWLNPEDPA RLREAMSDVV GDHNVVCPVA QLAGRLAAQG 450
    ARVYAYIFEH RASTLSWPLW MGVPHGYEIE FIFGLPLEPS LNYTAEERIF 500
    AQRLMRYWAN FARTGDPNDP RDPKVPQWPP YTAGAQQYVS LDLRPLEVRR 550
    GLRAQACAFW NRFLPKLLSA TDTLDEAERQ WKAEFHRWSS YMVHWKNQFD 600
    HYSKQDRCSD L 611
    Length:611
    Mass (Da):67,299
    Last modified:August 1, 1998 - v1
    Checksum:iDFA5C0885A225527
    GO
    Isoform H (identifier: O62763-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         572-611: DTLDEAERQW...YSKQDRCSDL → ASKAPSTCSG...FLLLSRLLRL

    Show »
    Length:613
    Mass (Da):66,628
    Checksum:i197901D5EB1B7402
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei572 – 61140DTLDE…RCSDL → ASKAPSTCSGPAHGEAAPRP RPGLSLPLLLLLFLLLSRLL RL in isoform H. CuratedVSP_001456Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF053485 Genomic DNA. Translation: AAC08995.1.
    AF053485 Genomic DNA. Translation: AAC08996.1.
    RefSeqiNP_001009203.1. NM_001009203.1. [O62763-1]

    Genome annotation databases

    GeneIDi493674.
    KEGGifca:493674.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF053485 Genomic DNA. Translation: AAC08995.1 .
    AF053485 Genomic DNA. Translation: AAC08996.1 .
    RefSeqi NP_001009203.1. NM_001009203.1. [O62763-1 ]

    3D structure databases

    ProteinModelPortali O62763.
    SMRi O62763. Positions 33-605.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S09.979.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 493674.
    KEGGi fca:493674.

    Organism-specific databases

    CTDi 43.

    Phylogenomic databases

    eggNOGi COG2272.
    HOVERGENi HBG008839.
    KOi K01049.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR014788. AChE_tetra.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    IPR000997. Cholinesterase.
    [Graphical view ]
    Pfami PF08674. AChE_tetra. 1 hit.
    PF00135. COesterase. 1 hit.
    [Graphical view ]
    PRINTSi PR00878. CHOLNESTRASE.
    ProDomi PD415333. AChE_tetra. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Determination of the DNA sequences of acetylcholinesterase and butyrylcholinesterase from cat and demonstration of the existence of both in cat plasma."
      Bartels C.F., Xie W., Miller-Lindholm A.K., Schopfer L.M., Lockridge O.
      Biochem. Pharmacol. 60:479-487(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS H AND T).

    Entry informationi

    Entry nameiACES_FELCA
    AccessioniPrimary (citable) accession number: O62763
    Secondary accession number(s): O62762
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2001
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3