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O62763 (ACES_FELCA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acetylcholinesterase

Short name=AChE
EC=3.1.1.7
Gene names
Name:ACHE
OrganismFelis catus (Cat) (Felis silvestris catus) [Reference proteome]
Taxonomic identifier9685 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis

Protein attributes

Sequence length611 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.

Catalytic activity

Acetylcholine + H2O = choline + acetate.

Subunit structure

Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers By similarity. Isoform H generates GPI-anchored dimers; disulfide linked. Isoform T generates multiple structures, ranging from monomers and dimers to collagen-tailed and hydrophobic-tailed forms, in which catalytic tetramers are associated with anchoring proteins that attach them to the basal lamina or to cell membranes. In the collagen-tailed forms, isoform T subunits are associated with a specific collagen, COLQ, which triggers the formation of isoform T tetramers, from monomers and dimers.

Subcellular location

Cell junctionsynapse. Secreted. Cell membrane; Peripheral membrane protein By similarity.

Isoform H: Cell membrane; Lipid-anchorGPI-anchor; Extracellular side.

Sequence similarities

Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform T (identifier: O62763-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform H (identifier: O62763-2)

The sequence of this isoform differs from the canonical sequence as follows:
     572-611: DTLDEAERQW...YSKQDRCSDL → ASKAPSTCSG...FLLLSRLLRL

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 By similarity
Chain32 – 611580Acetylcholinesterase
PRO_0000008586

Sites

Active site2311Acyl-ester intermediate By similarity
Active site3621Charge relay system By similarity
Active site4751Charge relay system By similarity

Amino acid modifications

Glycosylation2931N-linked (GlcNAc...) Potential
Glycosylation3781N-linked (GlcNAc...) Potential
Glycosylation4921N-linked (GlcNAc...) Potential
Disulfide bond97 ↔ 124 By similarity
Disulfide bond285 ↔ 300 By similarity
Disulfide bond437 ↔ 557 By similarity
Disulfide bond608Interchain By similarity

Natural variations

Alternative sequence572 – 61140DTLDE…RCSDL → ASKAPSTCSGPAHGEAAPRP RPGLSLPLLLLLFLLLSRLL RL in isoform H.
VSP_001456

Sequences

Sequence LengthMass (Da)Tools
Isoform T [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: DFA5C0885A225527

FASTA61167,299
        10         20         30         40         50         60 
MRPPWCPLYT PSLAAPILLL LLFLLGGGAE AEDPELLVTV RGGQLRGVRL MAPGGPVSAF 

        70         80         90        100        110        120 
LGIPFAEPPV GPRRFLPPEP KRPWPGVLDA TAFQSVCYQY VDTLYPGFEG TEMWNPNREL 

       130        140        150        160        170        180 
SEDCLYLNVW TPYPRPASPT PVLVWIYGGG FYSGASSLDV YDGRFLAQAE GTVLVSMNYR 

       190        200        210        220        230        240 
VGAFGFLALP GSREAPGNVG LLDQRLALQW VQDNVATFGG DPMSVTLFGE SAGAASVGMH 

       250        260        270        280        290        300 
LLSPPSRGLF HRAVLQSGAP NGPWATVGVG EARRRATLLA RLVGCPPGGA GGNDTELVAC 

       310        320        330        340        350        360 
LRTRPAQDLV DHEWHVLPQE SVFRFSFVPV VDGDFLSDTP EALINAGDFH GLQVLVGVVK 

       370        380        390        400        410        420 
DEGSYFLVYG APGFSKDNES LISRAQFLAG VRVGVPQASD LAAEAVVLHY TDWLNPEDPA 

       430        440        450        460        470        480 
RLREAMSDVV GDHNVVCPVA QLAGRLAAQG ARVYAYIFEH RASTLSWPLW MGVPHGYEIE 

       490        500        510        520        530        540 
FIFGLPLEPS LNYTAEERIF AQRLMRYWAN FARTGDPNDP RDPKVPQWPP YTAGAQQYVS 

       550        560        570        580        590        600 
LDLRPLEVRR GLRAQACAFW NRFLPKLLSA TDTLDEAERQ WKAEFHRWSS YMVHWKNQFD 

       610 
HYSKQDRCSD L 

« Hide

Isoform H [UniParc].

Checksum: 197901D5EB1B7402
Show »

FASTA61366,628

References

[1]"Determination of the DNA sequences of acetylcholinesterase and butyrylcholinesterase from cat and demonstration of the existence of both in cat plasma."
Bartels C.F., Xie W., Miller-Lindholm A.K., Schopfer L.M., Lockridge O.
Biochem. Pharmacol. 60:479-487(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS H AND T).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF053485 Genomic DNA. Translation: AAC08995.1.
AF053485 Genomic DNA. Translation: AAC08996.1.
RefSeqNP_001009203.1. NM_001009203.1.

3D structure databases

ProteinModelPortalO62763.
SMRO62763. Positions 33-605.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS09.979.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID493674.
KEGGfca:493674.

Organism-specific databases

CTD43.

Phylogenomic databases

eggNOGCOG2272.
HOVERGENHBG008839.
KOK01049.

Family and domain databases

InterProIPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamPF08674. AChE_tetra. 1 hit.
PF00135. COesterase. 1 hit.
[Graphical view]
PRINTSPR00878. CHOLNESTRASE.
ProDomPD415333. AChE_tetra. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACES_FELCA
AccessionPrimary (citable) accession number: O62763
Secondary accession number(s): O62762
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: August 1, 1998
Last modified: February 19, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families