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O62763

- ACES_FELCA

UniProt

O62763 - ACES_FELCA

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Protein

Acetylcholinesterase

Gene

ACHE

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.

Catalytic activityi

Acetylcholine + H2O = choline + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei231 – 2311Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei362 – 3621Charge relay systemBy similarity
Active sitei475 – 4751Charge relay systemBy similarity

GO - Molecular functioni

  1. acetylcholinesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. neurotransmitter catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Neurotransmitter degradation

Protein family/group databases

MEROPSiS09.979.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylcholinesterase (EC:3.1.1.7)
Short name:
AChE
Gene namesi
Name:ACHE
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
ProteomesiUP000011712: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. cell junction Source: UniProtKB-KW
  3. extracellular region Source: UniProtKB-KW
  4. plasma membrane Source: UniProtKB-KW
  5. synapse Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Secreted, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131By similarityAdd
BLAST
Chaini32 – 611580AcetylcholinesterasePRO_0000008586Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi97 ↔ 124By similarity
Disulfide bondi285 ↔ 300By similarity
Glycosylationi293 – 2931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi378 – 3781N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi437 ↔ 557By similarity
Glycosylationi492 – 4921N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi608 – 608InterchainBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Interactioni

Subunit structurei

Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers (By similarity). Isoform H generates GPI-anchored dimers; disulfide linked. Isoform T generates multiple structures, ranging from monomers and dimers to collagen-tailed and hydrophobic-tailed forms, in which catalytic tetramers are associated with anchoring proteins that attach them to the basal lamina or to cell membranes. In the collagen-tailed forms, isoform T subunits are associated with a specific collagen, COLQ, which triggers the formation of isoform T tetramers, from monomers and dimers.By similarity

Structurei

3D structure databases

ProteinModelPortaliO62763.
SMRiO62763. Positions 33-605.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2272.
HOVERGENiHBG008839.
InParanoidiO62763.
KOiK01049.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF08674. AChE_tetra. 1 hit.
PF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00878. CHOLNESTRASE.
ProDomiPD415333. AChE_tetra. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform T (identifier: O62763-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRPPWCPLYT PSLAAPILLL LLFLLGGGAE AEDPELLVTV RGGQLRGVRL
60 70 80 90 100
MAPGGPVSAF LGIPFAEPPV GPRRFLPPEP KRPWPGVLDA TAFQSVCYQY
110 120 130 140 150
VDTLYPGFEG TEMWNPNREL SEDCLYLNVW TPYPRPASPT PVLVWIYGGG
160 170 180 190 200
FYSGASSLDV YDGRFLAQAE GTVLVSMNYR VGAFGFLALP GSREAPGNVG
210 220 230 240 250
LLDQRLALQW VQDNVATFGG DPMSVTLFGE SAGAASVGMH LLSPPSRGLF
260 270 280 290 300
HRAVLQSGAP NGPWATVGVG EARRRATLLA RLVGCPPGGA GGNDTELVAC
310 320 330 340 350
LRTRPAQDLV DHEWHVLPQE SVFRFSFVPV VDGDFLSDTP EALINAGDFH
360 370 380 390 400
GLQVLVGVVK DEGSYFLVYG APGFSKDNES LISRAQFLAG VRVGVPQASD
410 420 430 440 450
LAAEAVVLHY TDWLNPEDPA RLREAMSDVV GDHNVVCPVA QLAGRLAAQG
460 470 480 490 500
ARVYAYIFEH RASTLSWPLW MGVPHGYEIE FIFGLPLEPS LNYTAEERIF
510 520 530 540 550
AQRLMRYWAN FARTGDPNDP RDPKVPQWPP YTAGAQQYVS LDLRPLEVRR
560 570 580 590 600
GLRAQACAFW NRFLPKLLSA TDTLDEAERQ WKAEFHRWSS YMVHWKNQFD
610
HYSKQDRCSD L
Length:611
Mass (Da):67,299
Last modified:August 1, 1998 - v1
Checksum:iDFA5C0885A225527
GO
Isoform H (identifier: O62763-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     572-611: DTLDEAERQW...YSKQDRCSDL → ASKAPSTCSG...FLLLSRLLRL

Show »
Length:613
Mass (Da):66,628
Checksum:i197901D5EB1B7402
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei572 – 61140DTLDE…RCSDL → ASKAPSTCSGPAHGEAAPRP RPGLSLPLLLLLFLLLSRLL RL in isoform H. CuratedVSP_001456Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053485 Genomic DNA. Translation: AAC08995.1.
AF053485 Genomic DNA. Translation: AAC08996.1.
RefSeqiNP_001009203.1. NM_001009203.1. [O62763-1]

Genome annotation databases

GeneIDi493674.
KEGGifca:493674.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053485 Genomic DNA. Translation: AAC08995.1 .
AF053485 Genomic DNA. Translation: AAC08996.1 .
RefSeqi NP_001009203.1. NM_001009203.1. [O62763-1 ]

3D structure databases

ProteinModelPortali O62763.
SMRi O62763. Positions 33-605.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S09.979.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 493674.
KEGGi fca:493674.

Organism-specific databases

CTDi 43.

Phylogenomic databases

eggNOGi COG2272.
HOVERGENi HBG008839.
InParanoidi O62763.
KOi K01049.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
InterProi IPR029058. AB_hydrolase.
IPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view ]
Pfami PF08674. AChE_tetra. 1 hit.
PF00135. COesterase. 1 hit.
[Graphical view ]
PRINTSi PR00878. CHOLNESTRASE.
ProDomi PD415333. AChE_tetra. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF53474. SSF53474. 1 hit.
PROSITEi PS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Determination of the DNA sequences of acetylcholinesterase and butyrylcholinesterase from cat and demonstration of the existence of both in cat plasma."
    Bartels C.F., Xie W., Miller-Lindholm A.K., Schopfer L.M., Lockridge O.
    Biochem. Pharmacol. 60:479-487(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS H AND T).

Entry informationi

Entry nameiACES_FELCA
AccessioniPrimary (citable) accession number: O62763
Secondary accession number(s): O62762
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3