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Protein

Cholinesterase

Gene

BCHE

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters (By similarity).By similarity

Catalytic activityi

An acylcholine + H2O = choline + a carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei226 – 2261Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei353 – 3531Charge relay systemBy similarity
Active sitei466 – 4661Charge relay systemBy similarity

GO - Molecular functioni

  1. acetylcholinesterase activity Source: UniProtKB
  2. cholinesterase activity Source: UniProtKB

GO - Biological processi

  1. metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Protein family/group databases

MEROPSiS09.980.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholinesterase (EC:3.1.1.8)
Alternative name(s):
Acylcholine acylhydrolase
Butyrylcholine esterase
Choline esterase II
Pseudocholinesterase
Gene namesi
Name:BCHE
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
ProteomesiUP000011712: Chromosome C2

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. blood microparticle Source: Ensembl
  2. endoplasmic reticulum Source: Ensembl
  3. nuclear envelope lumen Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 602574CholinesterasePRO_0000008612Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi85 – 851N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi93 ↔ 120By similarity
Glycosylationi134 – 1341N-linked (GlcNAc...)Sequence Analysis
Modified residuei226 – 2261PhosphoserineBy similarity
Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi280 ↔ 291By similarity
Glycosylationi284 – 2841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi369 – 3691N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi428 ↔ 547By similarity
Glycosylationi483 – 4831N-linked (GlcNAc...)Sequence Analysis
Glycosylationi509 – 5091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi513 – 5131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi514 – 5141N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi599 – 599InterchainBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Interactioni

Subunit structurei

Homotetramer; disulfide-linked. Dimer of dimers (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliO62760.
SMRiO62760. Positions 32-562, 564-593.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni144 – 1452Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00760000118946.
HOVERGENiHBG008839.
InParanoidiO62760.
KOiK01050.
OMAiSEMGNNA.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF08674. AChE_tetra. 1 hit.
PF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00878. CHOLNESTRASE.
ProDomiPD415333. AChE_tetra. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O62760-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSKGTIISI QFLLRFLLLW VLIGKSHTEE DIIITTKNGK VRGMNLPVLD
60 70 80 90 100
GTVTAFLGIP YAQPPLGRLR FKKPQFLTKW SDIWNATKYA NSCYQNADQS
110 120 130 140 150
FPGFPGSEMW NPNTDLSEDC LYLNVWIPTP KPKNATVMIW IYGGGFQTGT
160 170 180 190 200
SSLPVYDGKF LARVERVIVV SMNYRVGALG FLALPGNPEV PGNMGLFDQQ
210 220 230 240 250
LALQWVQKNI AAFGGNPKSV TLFGESAGAG SVSLHLLSPR SQPLFTRAIL
260 270 280 290 300
QSGSSNAPWA VMSLDEAKNR TLTLAKFIGC SKENDTEIIK CLRNKDPQEI
310 320 330 340 350
LLNELLVVPS DTLLSVNFGP VVDGDFLTDM PDTLLQLGQF KKTQILVGVN
360 370 380 390 400
KDEGTAFLVY GAPGFSKDND SIITRKEFQE GLKIYFPGVS EFGREAILFY
410 420 430 440 450
YVDLLDDQRA EKYREALDDV LGDYNIICPA LEFTTKFSEL GNNAFFYYFE
460 470 480 490 500
HRSSQLPWPE WMGVMHGYEI EFVFGLPLER RVNYTRAEEI LSRSIMNYWA
510 520 530 540 550
NFAKYGNPNG TQNNSTRWPA FRSTDQKYLT LNAESPKVYT KLRAQQCRFW
560 570 580 590 600
TLFFPKVLEM TGNIDEAERE WRAGFYRWNN YMMDWKNQFN DYTSKKESCA

GL
Length:602
Mass (Da):68,328
Last modified:August 1, 1998 - v1
Checksum:iECB8879232B74B9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053483 mRNA. Translation: AAC06261.1.
RefSeqiNP_001009364.1. NM_001009364.1.

Genome annotation databases

EnsembliENSFCAT00000026436; ENSFCAP00000016618; ENSFCAG00000030105.
GeneIDi493960.
KEGGifca:493960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053483 mRNA. Translation: AAC06261.1.
RefSeqiNP_001009364.1. NM_001009364.1.

3D structure databases

ProteinModelPortaliO62760.
SMRiO62760. Positions 32-562, 564-593.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS09.980.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSFCAT00000026436; ENSFCAP00000016618; ENSFCAG00000030105.
GeneIDi493960.
KEGGifca:493960.

Organism-specific databases

CTDi590.

Phylogenomic databases

GeneTreeiENSGT00760000118946.
HOVERGENiHBG008839.
InParanoidiO62760.
KOiK01050.
OMAiSEMGNNA.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF08674. AChE_tetra. 1 hit.
PF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00878. CHOLNESTRASE.
ProDomiPD415333. AChE_tetra. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Determination of the DNA sequences of acetylcholinesterase and butyrylcholinesterase from cat and demonstration of the existence of both in cat plasma."
    Bartels C.F., Xie W., Miller-Lindholm A.K., Schopfer L.M., Lockridge O.
    Biochem. Pharmacol. 60:479-487(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pituitary.

Entry informationi

Entry nameiCHLE_FELCA
AccessioniPrimary (citable) accession number: O62760
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: August 1, 1998
Last modified: March 4, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.