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O62760 (CHLE_FELCA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cholinesterase

EC=3.1.1.8
Alternative name(s):
Acylcholine acylhydrolase
Butyrylcholine esterase
Choline esterase II
Pseudocholinesterase
Gene names
Name:BCHE
OrganismFelis catus (Cat) (Felis silvestris catus) [Reference proteome]
Taxonomic identifier9685 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis

Protein attributes

Sequence length602 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters By similarity.

Catalytic activity

An acylcholine + H2O = choline + a carboxylate.

Subunit structure

Homotetramer; disulfide-linked. Dimer of dimers By similarity.

Subcellular location

Secreted By similarity.

Sequence similarities

Belongs to the type-B carboxylesterase/lipase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 602574Cholinesterase
PRO_0000008612

Regions

Region144 – 1452Substrate binding By similarity

Sites

Active site2261Acyl-ester intermediate By similarity
Active site3531Charge relay system By similarity
Active site4661Charge relay system By similarity

Amino acid modifications

Modified residue2261Phosphoserine By similarity
Glycosylation851N-linked (GlcNAc...) Potential
Glycosylation1341N-linked (GlcNAc...) Potential
Glycosylation2691N-linked (GlcNAc...) Potential
Glycosylation2841N-linked (GlcNAc...) Potential
Glycosylation3691N-linked (GlcNAc...) Potential
Glycosylation4831N-linked (GlcNAc...) Potential
Glycosylation5091N-linked (GlcNAc...) Potential
Glycosylation5131N-linked (GlcNAc...) Potential
Glycosylation5141N-linked (GlcNAc...) Potential
Disulfide bond93 ↔ 120 By similarity
Disulfide bond280 ↔ 291 By similarity
Disulfide bond428 ↔ 547 By similarity
Disulfide bond599Interchain By similarity

Sequences

Sequence LengthMass (Da)Tools
O62760 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: ECB8879232B74B9C

FASTA60268,328
        10         20         30         40         50         60 
MQSKGTIISI QFLLRFLLLW VLIGKSHTEE DIIITTKNGK VRGMNLPVLD GTVTAFLGIP 

        70         80         90        100        110        120 
YAQPPLGRLR FKKPQFLTKW SDIWNATKYA NSCYQNADQS FPGFPGSEMW NPNTDLSEDC 

       130        140        150        160        170        180 
LYLNVWIPTP KPKNATVMIW IYGGGFQTGT SSLPVYDGKF LARVERVIVV SMNYRVGALG 

       190        200        210        220        230        240 
FLALPGNPEV PGNMGLFDQQ LALQWVQKNI AAFGGNPKSV TLFGESAGAG SVSLHLLSPR 

       250        260        270        280        290        300 
SQPLFTRAIL QSGSSNAPWA VMSLDEAKNR TLTLAKFIGC SKENDTEIIK CLRNKDPQEI 

       310        320        330        340        350        360 
LLNELLVVPS DTLLSVNFGP VVDGDFLTDM PDTLLQLGQF KKTQILVGVN KDEGTAFLVY 

       370        380        390        400        410        420 
GAPGFSKDND SIITRKEFQE GLKIYFPGVS EFGREAILFY YVDLLDDQRA EKYREALDDV 

       430        440        450        460        470        480 
LGDYNIICPA LEFTTKFSEL GNNAFFYYFE HRSSQLPWPE WMGVMHGYEI EFVFGLPLER 

       490        500        510        520        530        540 
RVNYTRAEEI LSRSIMNYWA NFAKYGNPNG TQNNSTRWPA FRSTDQKYLT LNAESPKVYT 

       550        560        570        580        590        600 
KLRAQQCRFW TLFFPKVLEM TGNIDEAERE WRAGFYRWNN YMMDWKNQFN DYTSKKESCA 


GL 

« Hide

References

[1]"Determination of the DNA sequences of acetylcholinesterase and butyrylcholinesterase from cat and demonstration of the existence of both in cat plasma."
Bartels C.F., Xie W., Miller-Lindholm A.K., Schopfer L.M., Lockridge O.
Biochem. Pharmacol. 60:479-487(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pituitary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF053483 mRNA. Translation: AAC06261.1.
RefSeqNP_001009364.1. NM_001009364.1.

3D structure databases

ProteinModelPortalO62760.
SMRO62760. Positions 32-562, 564-593.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS09.980.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSFCAT00000026436; ENSFCAP00000016618; ENSFCAG00000030105.
GeneID493960.
KEGGfca:493960.

Organism-specific databases

CTD590.

Phylogenomic databases

GeneTreeENSGT00740000115241.
HOVERGENHBG008839.
KOK01050.
OMAWIPTPKP.

Family and domain databases

Gene3D3.40.50.1820. 1 hit.
InterProIPR029058. AB_hydrolase.
IPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamPF08674. AChE_tetra. 1 hit.
PF00135. COesterase. 1 hit.
[Graphical view]
PRINTSPR00878. CHOLNESTRASE.
ProDomPD415333. AChE_tetra. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF53474. SSF53474. 1 hit.
PROSITEPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHLE_FELCA
AccessionPrimary (citable) accession number: O62760
Entry history
Integrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: August 1, 1998
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families