O62760 (CHLE_FELCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cholinesterase
EC=3.1.1.8

Alternative name(s):
Acylcholine acylhydrolase
Butyrylcholine esterase
Choline esterase II
Pseudocholinesterase

Gene names

Name:

BCHE

Organism

Felis catus (Cat) (Felis silvestris catus) [Complete proteome]

Taxonomic identifier

9685 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis

Protein attributes

Sequence length	602 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters By similarity.
Catalytic activity	An acylcholine + H2O = choline + a carboxylate.
Subunit structure	Homotetramer; disulfide-linked. Dimer of dimers By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Serine esterase
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum Inferred from electronic annotation. Source: Compara extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro nuclear envelope lumen Inferred from electronic annotation. Source: Compara
Molecular_function	acetylcholinesterase activity Inferred from sequence or structural similarity. Source: UniProtKB carboxylesterase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 602	574	Cholinesterase		PRO_0000008612
Regions
	Region	144 – 145	2	Substrate binding By similarity
Sites
	Active site	226	1	Acyl-ester intermediate By similarity
	Active site	353	1	Charge relay system By similarity
	Active site	466	1	Charge relay system By similarity
Amino acid modifications
	Modified residue	226	1	Phosphoserine By similarity
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	134	1	N-linked (GlcNAc...) Potential
	Glycosylation	269	1	N-linked (GlcNAc...) Potential
	Glycosylation	284	1	N-linked (GlcNAc...) Potential
	Glycosylation	369	1	N-linked (GlcNAc...) Potential
	Glycosylation	483	1	N-linked (GlcNAc...) Potential
	Glycosylation	509	1	N-linked (GlcNAc...) Potential
	Glycosylation	513	1	N-linked (GlcNAc...) Potential
	Glycosylation	514	1	N-linked (GlcNAc...) Potential
	Disulfide bond	93 ↔ 120		By similarity
	Disulfide bond	280 ↔ 291		By similarity
	Disulfide bond	428 ↔ 547		By similarity
	Disulfide bond	599		Interchain By similarity

Sequences

	Sequence		Length	Mass (Da)
	O62760 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: ECB8879232B74B9C	FASTA	602	68,328
	10 20 30 40 50 60 MQSKGTIISI QFLLRFLLLW VLIGKSHTEE DIIITTKNGK VRGMNLPVLD GTVTAFLGIP 70 80 90 100 110 120 YAQPPLGRLR FKKPQFLTKW SDIWNATKYA NSCYQNADQS FPGFPGSEMW NPNTDLSEDC 130 140 150 160 170 180 LYLNVWIPTP KPKNATVMIW IYGGGFQTGT SSLPVYDGKF LARVERVIVV SMNYRVGALG 190 200 210 220 230 240 FLALPGNPEV PGNMGLFDQQ LALQWVQKNI AAFGGNPKSV TLFGESAGAG SVSLHLLSPR 250 260 270 280 290 300 SQPLFTRAIL QSGSSNAPWA VMSLDEAKNR TLTLAKFIGC SKENDTEIIK CLRNKDPQEI 310 320 330 340 350 360 LLNELLVVPS DTLLSVNFGP VVDGDFLTDM PDTLLQLGQF KKTQILVGVN KDEGTAFLVY 370 380 390 400 410 420 GAPGFSKDND SIITRKEFQE GLKIYFPGVS EFGREAILFY YVDLLDDQRA EKYREALDDV 430 440 450 460 470 480 LGDYNIICPA LEFTTKFSEL GNNAFFYYFE HRSSQLPWPE WMGVMHGYEI EFVFGLPLER 490 500 510 520 530 540 RVNYTRAEEI LSRSIMNYWA NFAKYGNPNG TQNNSTRWPA FRSTDQKYLT LNAESPKVYT 550 560 570 580 590 600 KLRAQQCRFW TLFFPKVLEM TGNIDEAERE WRAGFYRWNN YMMDWKNQFN DYTSKKESCA GL

References

[1]

"Determination of the DNA sequences of acetylcholinesterase and butyrylcholinesterase from cat and demonstration of the existence of both in cat plasma." Bartels C.F., Xie W., Miller-Lindholm A.K., Schopfer L.M., Lockridge O. Biochem. Pharmacol. 60:479-487(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pituitary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF053483 mRNA. Translation: AAC06261.1.
RefSeq	NP_001009364.1. NM_001009364.1.
3D structure databases
ProteinModelPortal	O62760.
SMR	O62760. Positions 32-562, 564-593.
ModBase	Search...
Protein family/group databases
MEROPS	S09.980.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	493960.
KEGG	fca:493960.
Organism-specific databases
CTD	590.
Phylogenomic databases
GeneTree	ENSGT00700000104419.
HOVERGEN	HBG008839.
KO	K01050.
Family and domain databases
InterPro	IPR014788. AChE_tetra. IPR002018. CarbesteraseB. IPR019826. Carboxylesterase_B_AS. IPR019819. Carboxylesterase_B_CS. IPR000997. Cholinesterase. [Graphical view]
Pfam	PF08674. AChE_tetra. 1 hit. PF00135. COesterase. 1 hit. [Graphical view]
PRINTS	PR00878. CHOLNESTRASE.
ProDom	PD415333. AChE_tetra. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00122. CARBOXYLESTERASE_B_1. 1 hit. PS00941. CARBOXYLESTERASE_B_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHLE_FELCA

Accession

Primary (citable) accession number: O62760

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
Last sequence update:	August 1, 1998
Last modified:	May 29, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program