Reviewed, UniProtKB/Swiss-Prot O62699 (NOS2_CANFA)

Names and origin

Protein names

Recommended name:
    Nitric oxide synthase, inducible
    EC=1.14.13.39
Alternative name(s):
    Inducible NO synthase
      Short name=Inducible NOS
      Short name=iNOS
    NOS type II

Gene names

Name:

NOS2

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	1154 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body.
Catalytic activity	L-arginine + n NADPH + m O(2) = citrulline + nitric oxide + n NADP(+).
Cofactor	Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity. Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.
Enzyme regulation	Regulated by calcium/calmodulin By similarity.
Subunit structure	Homodimer. Binds SLC9A3R1 By similarity.
Sequence similarities	Belongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Ligand	Calcium Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	defense response to bacterium Inferred from sequence or structural similarity. Source: UniProtKB nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW superoxide metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro FMN binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro nitric-oxide synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1154	1154	Nitric oxide synthase, inducible		PRO_0000170927
Regions
	Domain	536 – 674	139	Flavodoxin-like
	Domain	727 – 967	241	FAD-binding FR-type
	Nucleotide binding	620 – 651	32	FMN By similarity
	Nucleotide binding	764 – 775	12	FAD By similarity
	Nucleotide binding	900 – 910	11	FAD By similarity
	Nucleotide binding	975 – 993	19	NADP By similarity
	Nucleotide binding	1073 – 1088	16	NADP By similarity
	Region	506 – 526	21	Calmodulin-binding Potential
Sites
	Metal binding	197	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	42 – 52	11	DDLKNHKHHND → KCHSLSKHRDE Ref.2
	Sequence conflict	56	1	P → S in AAC15587. Ref.2
	Sequence conflict	59 – 64	6	ETVQKL → GTVKTS in AAC15587. Ref.2
	Sequence conflict	68 – 80	13	LDKLH…LSRPQ → TIKPAAPPLACPR Ref.2
	Sequence conflict	91 – 92	2	MT → RS in AAC78630. Ref.1
	Sequence conflict	102 – 104	3	KGD → MGV in AAC78630. Ref.1
	Sequence conflict	108	1	K → T in AAC78630. Ref.1
	Sequence conflict	111 – 115	5	SCLGA → LCMGS in AAC78630. Ref.1
	Sequence conflict	119	1	P → T in AAC78630. Ref.1
	Sequence conflict	125 – 127	3	EPR → GPS in AAC78630. Ref.1
	Sequence conflict	133 – 134	2	PD → TE in AAC78630. Ref.1
	Sequence conflict	149	1	S → G in AAC78630. Ref.1
	Sequence conflict	169	1	E → D in AAC78630. Ref.1
	Sequence conflict	394 – 395	2	RR → SK in AAC78630. Ref.1
	Sequence conflict	497 – 499	3	VWQ → LWL in AAC78630. Ref.1
	Sequence conflict	506 – 508	3	QRR → HRK in AAC78630. Ref.1
	Sequence conflict	615	1	K → N in AAC78630. Ref.1
	Sequence conflict	624 – 626	3	GSS → RSN in AAC78630. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O62699-1 [UniParc]. Last modified September 13, 2005. Version 2. Checksum: D6AD3A88AE89E995	FASTA	1,154	131,847
	10 20 30 40 50 60 MACPWKFLFR AKFHQYGMKE EKDINNNVEK PPGATPSPST QDDLKNHKHH NDSPQPLTET 70 80 90 100 110 120 VQKLPESLDK LHATPLSRPQ HVRIKNWGNG MTFQDTLHHK AKGDLACKSK SCLGAIMNPK 130 140 150 160 170 180 SLTREPRDKP TPPDELLPQA IEFVNQYYSS FKEAKIEEHL ARVEAVTKEI ETTGTYQLTG 190 200 210 220 230 240 DELIFATKQA WRNAPRCIGR IQWSNLQVFD ARSCSTAKEM FEHICRHLRY ASNNGNIRSA 250 260 270 280 290 300 ITVFPQRTDG KHDFRVWNAQ LIRYAGYQMP DGTILGDPAS VEFTQLCIDL GWKPKYGRFD 310 320 330 340 350 360 VVPLVLQADG QDPEFFEIPP DLVLEVPMEH PKYEWFRELE LKWYALPAVA NMLLEVGGLE 370 380 390 400 410 420 FPGCPFNGWY MGTEIGVRDF CDVQRYNILE EVGRRMGLET HKLASLWKDR AVIEINVAVL 430 440 450 460 470 480 HSFQKQNVTI MDHHSAAESF MKYMQSEYRS RGGCPADWIW LVPPISGSIT PVFHQEMLNY 490 500 510 520 530 540 VLSPFYYYQV EAWKTHVWQD EKRRPQRRKI QLKVLVKAVL FASMLMRKTM ASRVRVTILF 550 560 570 580 590 600 ATETGKSETL ARDLGALFSC AFHPKVLCMD EYKLSHLEEE QLLLVVTSTF GNGDSPGNGE 610 620 630 640 650 660 KLKKSLFMLK ELTNKFRYAV FGLGSSMYPQ FCAFAHDIDH KLSHLGASQL TPGGEGDELN 670 680 690 700 710 720 GKEEAFRCWA VQTFKAACDT SDVRGKHCIQ IPRLYTSNVT WDPHHYRLLQ DSQPLDLNKA 730 740 750 760 770 780 LSKMHAKNVF TLRLKSQRNL QSPISNRTTL QVELSCEDSQ ELSYLPGEHL GVFPGNQLAL 790 800 810 820 830 840 VQGILERVVY SPAPLQPVHL ETLSERGSYW VRNNRLPPCS LSQALTYFLD ITTPPTHLLL 850 860 870 880 890 900 RKLAQLAHQY AERHRLEILC HPSEYNKWKL TNSPTFLEVL EEFPSLRVSA GFLLSQLPIL 910 920 930 940 950 960 KPRYYSISSS RDCTPMEVHL TVAVLVYPTR DGQGPLHHGV CSTWLSNLKP QDPVPCFVRS 970 980 990 1000 1010 1020 AGNFKLPEDP SRPCILIGPG TGIAPFRSFW QQRLHDIKHK GLRGSRMTLV FGCRRPDEDH 1030 1040 1050 1060 1070 1080 LYREEMLEMA QSGVLHEVHT AYSRLPGQPK VYVQDILRQQ LASQVLRMLH EEQGHLYVCG 1090 1100 1110 1120 1130 1140 DVRMARDVAH TLKHLVAAKL SLSEEQVEDY FFQLKSQKRY HEDIFGAVFP YEVKKDGAAK 1150 QPSDPRVPAA HGRS

References

[1]	"Induction and cDNA sequence of inducible nitric oxide synthase from canine aortic smooth muscle cells." Wang X., McGregor C.G.A., Miller V.M. Am. J. Physiol. 275:H1122-H1129(1998) [PubMed: 9746458] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Aorta.
[2]	"The canine inducible NO synthase." Haerter L., Straubinger R.K., Appel M.J.G. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 42-632. Tissue: Alveolar macrophage.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF077821 mRNA. Translation: AAC78630.1. AF032909 mRNA. Translation: AAC15587.1.
RefSeq	NP_001003186.1.
UniGene	Cfa.45041
3D structure databases
HSSP	HSSP built from PDB template 1NOS based on UniProtKB P29477.
SMR	O62699. Positions 80-499.
ModBase	Search...
Genome annotation databases
Ensembl	ENSCAFG00000018642. Canis familiaris. [Contig view]
GeneID	403822.
KEGG	cfa:403822.
Phylogenomic databases
HOVERGEN	O62699.
Family and domain databases
InterPro	IPR003097. FAD-binding_1. IPR001094. Flavdoxin_like. IPR008254. Flavodoxin/NO_synth. IPR001709. FPN_cyt_redctse. IPR004030. NO_synthase_oxygenase_reg. IPR012144. NOS. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Gene3D	G3DSA:3.90.340.10. NO_synthase_oxygenase_reg. 1 hit.
Pfam	PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. PF02898. NO_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000333. NOS. 1 hit.
PRINTS	PR00369. FLAVODOXIN. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. PS60001. NOS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NOS2_CANFA

Accession

Primary (citable) accession number: O62699
Secondary accession number(s): O97604

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	September 13, 2005
Last modified:	November 25, 2008
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)